##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34139-1.342000000356e9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2829985 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.13863006341023 33.0 33.0 33.0 27.0 33.0 2 30.681258027869404 33.0 33.0 33.0 27.0 33.0 3 30.53224275040327 33.0 33.0 33.0 27.0 33.0 4 33.98353383498499 37.0 37.0 37.0 27.0 37.0 5 34.05752362645032 37.0 37.0 37.0 27.0 37.0 6 34.51626775406937 37.0 37.0 37.0 27.0 37.0 7 34.16913517209455 37.0 37.0 37.0 27.0 37.0 8 34.19968904428822 37.0 37.0 37.0 27.0 37.0 9 33.77761507569828 37.0 37.0 37.0 22.0 37.0 10-11 33.80573306925655 37.0 37.0 37.0 24.5 37.0 12-13 33.80035971922113 37.0 37.0 37.0 24.5 37.0 14-15 35.43585831727023 40.0 37.0 40.0 22.0 40.0 16-17 35.36429910405886 40.0 37.0 40.0 24.5 40.0 18-19 35.391108433436926 40.0 37.0 40.0 22.0 40.0 20-21 35.35469004252673 40.0 37.0 40.0 22.0 40.0 22-23 36.0113638411511 40.0 37.0 40.0 27.0 40.0 24-25 35.445676390510904 40.0 37.0 40.0 22.0 40.0 26-27 35.190011431155995 40.0 37.0 40.0 18.5 40.0 28-29 35.005429887437565 40.0 37.0 40.0 18.5 40.0 30-31 35.02839909045454 40.0 37.0 40.0 18.5 40.0 32-33 34.81727535658317 40.0 37.0 40.0 15.0 40.0 34-35 34.96308902697364 38.5 37.0 40.0 18.5 40.0 36-37 34.728710222845706 37.0 37.0 40.0 15.0 40.0 38-39 34.613018443560655 37.0 37.0 40.0 15.0 40.0 40-41 34.98604356560194 37.0 37.0 40.0 22.0 40.0 42-43 34.811766493461974 37.0 37.0 40.0 22.0 40.0 44-45 34.414749901501246 37.0 37.0 40.0 15.0 40.0 46-47 34.22932754060534 37.0 37.0 40.0 15.0 40.0 48-49 34.088544108891035 37.0 33.0 40.0 15.0 40.0 50-51 33.095791497128076 37.0 33.0 38.5 15.0 38.5 52-53 33.52311284335429 37.0 33.0 38.5 15.0 40.0 54-55 34.21771581828172 37.0 33.0 40.0 15.0 40.0 56-57 34.499174377249346 37.0 33.0 40.0 22.0 40.0 58-59 34.062859697136204 37.0 33.0 40.0 15.0 40.0 60-61 33.78171898437624 37.0 33.0 40.0 15.0 40.0 62-63 33.42870085883847 37.0 33.0 40.0 15.0 40.0 64-65 33.205291900840464 37.0 33.0 40.0 15.0 40.0 66-67 32.99896130191503 37.0 33.0 37.0 15.0 40.0 68-69 32.99781429936908 37.0 33.0 37.0 15.0 40.0 70-71 32.65374675130787 37.0 33.0 37.0 15.0 40.0 72-73 32.731860592900674 37.0 33.0 37.0 18.5 38.5 74-75 32.802589236338704 37.0 33.0 37.0 22.0 37.0 76-77 32.58359849963869 37.0 33.0 37.0 22.0 37.0 78-79 32.21634231276845 37.0 33.0 37.0 22.0 37.0 80-81 31.76019289854893 37.0 33.0 37.0 15.0 37.0 82-83 31.435442767364492 37.0 33.0 37.0 15.0 37.0 84-85 31.12108562412875 33.0 33.0 37.0 15.0 37.0 86-87 30.95349339307452 33.0 33.0 37.0 15.0 37.0 88-89 30.83061323646592 33.0 33.0 37.0 15.0 37.0 90-91 30.691750662989378 33.0 33.0 37.0 15.0 37.0 92-93 30.522369023157367 33.0 27.0 37.0 15.0 37.0 94-95 30.375955349586658 33.0 27.0 37.0 15.0 37.0 96-97 30.240676364008998 33.0 27.0 37.0 15.0 37.0 98-99 30.092289711782925 33.0 27.0 37.0 15.0 37.0 100-101 28.839346498302994 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.07774763272356822 2306 2 0.23161664090386225 2306 3 0.2666545238613871 2306 4 0.32780336174150193 2306 5 0.3146319969562086 2306 6 0.06057761560415997 2306 7 0.2790992655245006 2306 8 0.05371024048918116 2306 9 0.3339814234440226 2306 10-11 0.30315084885067733 2306 12-13 0.30256741465847625 2306 14-15 0.5937707057041877 2306 16-17 0.5354125665656326 2306 18-19 0.5278562829206876 2306 20-21 0.47144503126466475 2306 22-23 0.20516833482695773 2306 24-25 0.655828300559584 2306 26-27 0.17723043853801101 2306 28-29 0.2557085138973747 2306 30-31 0.3464863427773892 2306 32-33 0.4605936583195387 2306 34-35 0.1984578082829742 2306 36-37 0.3848361340584674 2306 38-39 0.3283984797028623 2306 40-41 -0.006144830055959005 2306 42-43 0.3094861101252704 2306 44-45 0.6192116686896512 2306 46-47 0.6648605722016896 2306 48-49 0.5116265907713142 2306 50-51 0.22862984660840624 2306 52-53 0.17008434625121538 2306 54-55 0.2928200132793748 2306 56-57 0.10674294687379415 2306 58-59 0.32403629331359696 2306 60-61 0.4252872730242174 2306 62-63 0.34224781096286705 2306 64-65 0.3506707498838111 2306 66-67 0.30213819040397993 2306 68-69 0.12492777913018216 2306 70-71 0.31661603301525076 2306 72-73 0.10022777297606922 2306 74-75 -0.12645337119556643 2306 76-77 0.07443333130993324 2306 78-79 0.20807172288427012 2306 80-81 0.4033551756738625 2306 82-83 0.393497269220628 2306 84-85 0.4969435606461836 2306 86-87 0.4672174244022038 2306 88-89 0.3009806143495588 2306 90-91 0.13335902858183957 2306 92-93 0.33606424463531326 2306 94-95 0.07620976332595575 2306 96-97 0.09962582416745036 2306 98-99 0.25266765125032364 2306 100-101 0.045980596119196804 2307 1 0.14419390302181512 2307 2 0.224559428421923 2307 3 0.10367119376283185 2307 4 0.2114828165731879 2307 5 0.2556544032014898 2307 6 0.012236410971958378 2307 7 0.32017838825206724 2307 8 -0.07658261495927121 2307 9 0.26380803921680496 2307 10-11 0.22830288352751893 2307 12-13 0.20330257240794225 2307 14-15 0.5737432100711359 2307 16-17 0.4115559535963129 2307 18-19 0.45706678086121855 2307 20-21 0.31082503169596265 2307 22-23 0.16764898005781248 2307 24-25 0.3577607583456981 2307 26-27 0.06033377898786085 2307 28-29 0.10017154035787712 2307 30-31 0.15267631064524068 2307 32-33 0.391520099414187 2307 34-35 0.07214761441180428 2307 36-37 0.28566089522384885 2307 38-39 0.10039811309441404 2307 40-41 -0.0254161418672183 2307 42-43 0.2864491904987787 2307 44-45 0.3738716932739834 2307 46-47 0.4535854217844033 2307 48-49 0.20939060276156596 2307 50-51 -0.010596933059915159 2307 52-53 0.07236950292241318 2307 54-55 0.2411789013775376 2307 56-57 0.0834456274284463 2307 58-59 0.32499335699563403 2307 60-61 0.34675176591849066 2307 62-63 0.5267726217282203 2307 64-65 0.566468985850257 2307 66-67 0.39211069477092764 2307 68-69 0.21376994341388666 2307 70-71 0.21585683006512824 2307 72-73 -0.188996095389939 2307 74-75 -0.05634435909748703 2307 76-77 -0.03488197351420297 2307 78-79 0.05535698738147943 2307 80-81 0.24670360464886798 2307 82-83 0.4785482709242821 2307 84-85 0.33907150699196364 2307 86-87 0.429238515170141 2307 88-89 0.31611927860092237 2307 90-91 0.3669130817616306 2307 92-93 0.30786418425274675 2307 94-95 0.22096510610484899 2307 96-97 0.21350594320736604 2307 98-99 0.20080528037197354 2307 100-101 0.13628455764687786 2201 1 0.08850117572184146 2201 2 0.11312362090195549 2201 3 0.0056598343838523135 2201 4 0.028363033570293794 2201 5 0.016948593133903955 2201 6 -0.07713822374458346 2201 7 0.24295379324839672 2201 8 -0.17350539216258198 2201 9 0.12923746270584502 2201 10-11 0.12244336217997898 2201 12-13 0.06972856410569506 2201 14-15 0.3082466493554108 2201 16-17 0.18133401111253988 2201 18-19 0.1782362873726271 2201 20-21 0.13970962460160052 2201 22-23 0.06644021025757496 2201 24-25 0.31653520423878945 2201 26-27 0.008029083877794108 2201 28-29 0.11671936813890227 2201 30-31 0.06099143600302881 2201 32-33 0.2104929597897396 2201 34-35 0.09834491993114369 2201 36-37 0.1326167914608689 2201 38-39 0.07451761136353952 2201 40-41 -0.03926139655408889 2201 42-43 0.171954443087607 2201 44-45 0.2753738887212407 2201 46-47 0.21560733129900456 2201 48-49 0.10484295481239769 2201 50-51 -0.11956844469654015 2201 52-53 0.017561848079679976 2201 54-55 0.17716775180239352 2201 56-57 0.043615568533510896 2201 58-59 0.22305686761320942 2201 60-61 0.0891423307403798 2201 62-63 0.13857708455547169 2201 64-65 0.1330559270602194 2201 66-67 0.08951721967014947 2201 68-69 -0.016172060812159827 2201 70-71 0.017139930366347755 2201 72-73 -0.13194593728776027 2201 74-75 -0.14211066759903446 2201 76-77 0.1676377705251184 2201 78-79 0.26011563804311066 2201 80-81 0.18465651867425592 2201 82-83 0.29248074406189417 2201 84-85 0.31355696309050884 2201 86-87 0.2546799274496614 2201 88-89 0.2895659060043023 2201 90-91 0.22571150523568306 2201 92-93 0.20671565812467563 2201 94-95 0.18826867726581042 2201 96-97 0.11437153388008525 2201 98-99 0.23722632669366206 2201 100-101 0.12151376833488214 1101 1 0.10051769028072854 1101 2 0.020327010732462014 1101 3 -0.022273672786941034 1101 4 0.18449080410483987 1101 5 0.07128366484185733 1101 6 -0.07501957967678408 1101 7 0.3149007728224973 1101 8 0.040119118919555774 1101 9 0.2014234687901606 1101 10-11 0.15248464857720023 1101 12-13 0.11825094567893046 1101 14-15 0.3633150978560664 1101 16-17 0.22433162084743685 1101 18-19 0.27282559636871184 1101 20-21 0.2619608197341492 1101 22-23 0.18055907162219142 1101 24-25 0.24182855495586608 1101 26-27 -0.04324210256288552 1101 28-29 0.05986908565302684 1101 30-31 0.12445840688526033 1101 32-33 0.2299464582686639 1101 34-35 0.12601767090897908 1101 36-37 0.18608267585896243 1101 38-39 0.08277489080725786 1101 40-41 -0.04233071424204837 1101 42-43 0.21710329141442486 1101 44-45 0.4089082216199813 1101 46-47 0.15616578414559257 1101 48-49 0.07444856107053965 1101 50-51 0.16137462353466958 1101 52-53 0.10460552474200568 1101 54-55 0.2310410677519812 1101 56-57 0.19605472542182412 1101 58-59 0.2838619523589685 1101 60-61 0.18482355629448222 1101 62-63 0.19200494200875795 1101 64-65 0.24448574018494895 1101 66-67 0.21384359950500453 1101 68-69 0.11747635292099545 1101 70-71 0.16415753140937284 1101 72-73 -0.14626036579711155 1101 74-75 0.00878568181425976 1101 76-77 0.031001525414296793 1101 78-79 0.2505654424759669 1101 80-81 0.26082448912189093 1101 82-83 0.12722107435307706 1101 84-85 0.407499814024888 1101 86-87 0.3428752772975514 1101 88-89 0.4752786395983897 1101 90-91 0.2478540519545014 1101 92-93 0.30537928263575864 1101 94-95 0.15791231046876675 1101 96-97 0.2796529332716524 1101 98-99 0.21979368871017613 1101 100-101 0.18540446803066502 2304 1 0.05835340328793137 2304 2 -0.0193923162581342 2304 3 -0.03906483058892363 2304 4 0.06971227211349174 2304 5 0.0051637265252040265 2304 6 0.18637910404459035 2304 7 0.28783238416231427 2304 8 0.08266479626606582 2304 9 0.13244413546600242 2304 10-11 0.03075813437488506 2304 12-13 0.07425199209289701 2304 14-15 0.23295859425557097 2304 16-17 0.16378159950610183 2304 18-19 0.15123711242979 2304 20-21 0.20243982949609318 2304 22-23 0.023995749808598532 2304 24-25 0.12123760643667936 2304 26-27 0.023474016342781567 2304 28-29 0.08518008401456001 2304 30-31 0.18499775755785208 2304 32-33 0.11335236362327095 2304 34-35 -0.011648757101873741 2304 36-37 0.26888740950530376 2304 38-39 0.13889228193201575 2304 40-41 0.111437925006733 2304 42-43 0.30433101725396483 2304 44-45 0.16832784050500038 2304 46-47 0.07841412847086104 2304 48-49 0.06307483294541782 2304 50-51 0.11203413970589082 2304 52-53 0.1606872896017677 2304 54-55 0.26487517671672123 2304 56-57 0.2662214288047693 2304 58-59 0.1775344179030327 2304 60-61 0.22084618499651754 2304 62-63 0.1965700950468019 2304 64-65 0.24604021566995726 2304 66-67 0.2733731951210885 2304 68-69 0.20503813953516214 2304 70-71 0.26496660905053915 2304 72-73 0.2311155790733963 2304 74-75 0.2457411334933468 2304 76-77 0.3533844578517531 2304 78-79 0.4118340128817266 2304 80-81 0.2994817403576171 2304 82-83 0.26640600736881126 2304 84-85 0.42786409688710236 2304 86-87 0.3573675944160577 2304 88-89 0.33017578812032866 2304 90-91 0.2293758958460579 2304 92-93 0.25318973025310143 2304 94-95 0.29295685190538734 2304 96-97 0.3380447258063164 2304 98-99 0.3075274107771193 2304 100-101 0.2603144557766086 2202 1 0.10193595336280836 2202 2 -0.16362951920116586 2202 3 -0.15812570762761524 2202 4 -0.06999217269945746 2202 5 -0.0789771690187564 2202 6 0.058306910273536516 2202 7 0.08831456639016011 2202 8 0.017077560512959167 2202 9 0.02519940072429705 2202 10-11 -0.02112468624596886 2202 12-13 -0.0014418425172451066 2202 14-15 0.04116727405185827 2202 16-17 0.04095111304597765 2202 18-19 1.5963872140645208E-4 2202 20-21 0.08352622676959953 2202 22-23 0.09388700877975253 2202 24-25 0.033701981538705184 2202 26-27 0.01922306349572267 2202 28-29 -0.05241316014333819 2202 30-31 0.03337135232218458 2202 32-33 -0.1459573731283328 2202 34-35 -0.11383067523606627 2202 36-37 0.03368468714044326 2202 38-39 -0.07276623007156502 2202 40-41 -0.14127172876797545 2202 42-43 0.16143444608385238 2202 44-45 0.05728381564806284 2202 46-47 -0.1374654943778495 2202 48-49 -0.011058668234390723 2202 50-51 -0.056520262102310426 2202 52-53 -0.04101431747875495 2202 54-55 0.11556789830083858 2202 56-57 0.3518178075020302 2202 58-59 0.1422151988149949 2202 60-61 -0.013779215612196083 2202 62-63 0.10412328317271857 2202 64-65 0.15919273120553612 2202 66-67 0.08154429012575548 2202 68-69 0.1661190071663654 2202 70-71 0.24260575504664672 2202 72-73 0.14184073004167175 2202 74-75 0.20716800703874583 2202 76-77 0.3301210849248051 2202 78-79 0.3353815461902414 2202 80-81 0.3039746553824436 2202 82-83 0.39677200258505607 2202 84-85 0.14699359439941162 2202 86-87 0.018654581561090566 2202 88-89 0.009485570038204827 2202 90-91 0.09609990217191822 2202 92-93 0.1600860717204533 2202 94-95 0.2589253555273956 2202 96-97 0.16340432999490773 2202 98-99 0.18046145704932215 2202 100-101 0.24696782205121082 1102 1 -0.12111991488937335 1102 2 -0.21964709383758318 1102 3 -0.07422032242530463 1102 4 -0.14159747781199883 1102 5 -0.15324591755823747 1102 6 -0.052552005119125056 1102 7 -0.09215131966943346 1102 8 0.04956308916708707 1102 9 -0.16833182421005688 1102 10-11 -0.07309679527752877 1102 12-13 -0.05885422121342998 1102 14-15 -0.11623994677906069 1102 16-17 -0.1484506618010215 1102 18-19 -0.1691734538113039 1102 20-21 -0.06516360026597567 1102 22-23 -0.039477717857906214 1102 24-25 -0.09746832154828411 1102 26-27 0.024342883863674558 1102 28-29 0.03476423643694915 1102 30-31 0.14615048230609773 1102 32-33 -0.13466721207962706 1102 34-35 -0.013370497414520344 1102 36-37 0.06795539356347291 1102 38-39 -0.14963579081693013 1102 40-41 -0.018479723669656778 1102 42-43 0.08866508747266977 1102 44-45 -0.06431864737545112 1102 46-47 -0.15702628485754389 1102 48-49 -0.13408465695806626 1102 50-51 0.028582409491448857 1102 52-53 -0.053621087545323576 1102 54-55 0.00393116780396241 1102 56-57 0.232999915032849 1102 58-59 0.0379927868528398 1102 60-61 -0.07146956264480053 1102 62-63 0.07848500862450436 1102 64-65 -0.009765025126078797 1102 66-67 0.16530057843569068 1102 68-69 0.1988465645126567 1102 70-71 0.13627124989043438 1102 72-73 0.1387084923010704 1102 74-75 0.36656754731205154 1102 76-77 0.28195038274235174 1102 78-79 0.1936245366814191 1102 80-81 0.09566659759390106 1102 82-83 0.12880135339478116 1102 84-85 0.04550071571210168 1102 86-87 -0.015386953239161727 1102 88-89 0.09929641021686209 1102 90-91 0.03085516221704765 1102 92-93 0.14344797130658904 1102 94-95 0.1727862988783997 1102 96-97 0.2639604471178494 1102 98-99 0.08762679956315367 1102 100-101 2.1532229105858391E-4 2305 1 0.04402236953254146 2305 2 -0.21813279220736703 2305 3 -0.01060984952296451 2305 4 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-0.15174875790209796 1213 58-59 -0.32728609585031165 1213 60-61 -0.1618492121288071 1213 62-63 -0.22568787658624956 1213 64-65 -0.01417258304307012 1213 66-67 0.13292389275959948 1213 68-69 0.19094528256026422 1213 70-71 0.041388760986613704 1213 72-73 0.32547812571905865 1213 74-75 0.3409634998075788 1213 76-77 0.09181482554667042 1213 78-79 0.03280238811093028 1213 80-81 -0.10755680201289763 1213 82-83 -0.06057236537144561 1213 84-85 -0.05884452406951368 1213 86-87 0.06332823437389123 1213 88-89 0.007705811931746354 1213 90-91 -0.012386716727984748 1213 92-93 0.02167097283006214 1213 94-95 0.08554887102195252 1213 96-97 0.10572914891745455 1213 98-99 0.09352215514048368 1213 100-101 0.270241757091938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1297.0 3 403.0 4 415.0 5 366.0 6 919.0 7 4595.0 8 23179.0 9 58266.0 10 69667.0 11 40945.0 12 19944.0 13 11953.0 14 8913.0 15 7484.0 16 7195.0 17 6943.0 18 7151.0 19 7591.0 20 8093.0 21 9297.0 22 10379.0 23 11850.0 24 13675.0 25 15787.0 26 19475.0 27 23770.0 28 29309.0 29 35064.0 30 43016.0 31 54307.0 32 74193.0 33 105915.0 34 160657.0 35 270468.0 36 517018.0 37 870397.0 38 279210.0 39 879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.23795662240027 23.908711490162766 19.951491594487024 14.901840292949945 2 33.71904744357961 25.028380262926657 27.83019603285733 13.422376260636398 3 37.28968745063398 20.899990011681375 25.33937260607984 16.47094993160481 4 30.804003531652953 19.069190577867246 30.26975779561391 19.857048094865892 5 34.700760918996345 16.90756768013918 29.053901852641538 19.33776954822294 6 33.15199229701793 17.11219591628671 32.01282886352695 17.722982923168416 7 37.19622801485977 18.2338211803307 25.089511749589334 19.480439055220202 8 30.376434835350015 29.49393014840403 21.526582007930827 18.603053008315126 9 32.10998138721007 26.3454648439034 22.30775245166282 19.236801317223712 10-11 27.296261722244488 29.990940917164043 26.452026655847177 16.260770704744292 12-13 29.31475069307207 25.476057502117115 26.311476700377217 18.89771510443359 14-15 27.152439374975124 25.509860550280184 27.56027120383188 19.777428870912814 16-17 28.03042672598699 25.956013921996135 28.043358084531462 17.970201267485418 18-19 27.63411479705904 26.079848395659877 28.416403946184282 17.869632861096804 20-21 26.122618502427684 27.639602407292134 28.035263507851933 18.202515582428248 22-23 28.336068525181606 26.083626979401576 27.242493114836474 18.337811380580344 24-25 25.833591098459646 28.324819237208292 27.386595633053084 18.454994031278975 26-27 23.37778355431541 30.71940086759031 27.49933331751475 18.403482260579533 28-29 24.49477081128946 29.800471713060517 27.096911134506556 18.607846341143468 30-31 26.527309338076314 27.728271284282535 27.21152024678446 18.532899130856688 32-33 23.88402369045568 29.387754306179215 28.48363924878595 18.24458275457916 34-35 26.969663932210302 27.784221699262986 27.463603366439308 17.782511002087407 36-37 24.653874003702906 28.868911985521617 28.888233036693155 17.588980974082315 38-39 23.27931670088575 31.174777552329314 27.491933313739054 18.053972433045885 40-41 27.322770449713495 27.013460683108704 27.01207793655923 18.651690930618564 42-43 27.415691411889497 26.848117332329135 27.142870060055092 18.593321195726272 44-45 25.022734081268354 28.053625842224587 28.275489203748776 18.64815087275828 46-47 25.062696277689415 28.641024827282724 27.643947905487785 18.652330989540072 48-49 22.264737731528594 29.554923308978758 28.60677209835342 19.57356686113922 50-51 20.770952204835506 28.11397976736261 30.11151744071214 21.003550587089734 52-53 24.893252032275317 27.393513063341825 27.434763949030426 20.278470955352432 54-55 26.608891498112918 27.169325168163784 27.28888506013623 18.932898273587064 56-57 27.189833233056422 27.003608905156952 26.62963570940567 19.17692215238096 58-59 26.03936950509202 27.3612228579309 26.8586058995585 19.74080173741858 60-61 22.91325741988558 27.40625036551379 26.80967869876028 22.87081351584035 62-63 24.476972656585705 27.45086643029796 26.648607950855535 21.423552962260807 64-65 23.00055964588132 28.28531448802326 28.370342383481834 20.34378348261359 66-67 21.561897489942435 28.281899645198123 27.953063635696562 22.203139229162876 68-69 21.145822423097567 27.529858823099907 31.533430202935257 19.790888550867265 70-71 20.37293925097112 30.319568812357574 30.08789706013419 19.21959487653712 72-73 20.064852215016224 33.77756445045446 26.846375446521197 19.311207888008116 74-75 20.336560667040963 33.953352983519395 26.377176917757822 19.332909431681824 76-77 20.602475363564704 33.470365319609705 26.22653313208772 19.700626184737875 78-79 20.272004314873804 33.36932605257441 26.72578119379829 19.632888438753493 80-81 19.69400716330652 33.31897978524257 26.925696820458732 20.061316230992183 82-83 19.602698916406474 32.92661355044906 26.86957813302025 20.601109400124212 84-85 19.60794252323753 32.40039989444375 27.36921596125398 20.622441621064745 86-87 19.947334308925857 32.01407986660955 27.947517919290483 20.09106790517411 88-89 20.420112512096644 32.236895743753166 27.571135365451628 19.77185637869856 90-91 20.834225391253057 31.68109437429285 26.937283956568713 20.547396277885376 92-93 20.676969584743908 31.876435862518683 26.7662581943284 20.680336358409004 94-95 20.318366975198334 31.927561298054986 27.086159167894753 20.667912558851928 96-97 20.563999403831605 32.32072725455416 26.7087694350631 20.406503906551134 98-99 20.959056823478743 31.71633101213886 26.636690233082426 20.687921931299968 100-101 21.107127956393494 31.664591196105558 26.27921477380561 20.949066073695334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 762.0 1 576.0 2 289.0 3 193.5 4 197.0 5 233.5 6 319.5 7 441.0 8 582.0 9 708.5 10 848.5 11 930.5 12 915.5 13 844.0 14 722.0 15 601.5 16 532.5 17 478.5 18 458.0 19 513.0 20 586.5 21 695.5 22 920.5 23 1245.5 24 1744.5 25 2498.5 26 3510.0 27 4761.0 28 6394.5 29 8499.0 30 10892.0 31 14333.0 32 20181.5 33 29395.0 34 42970.0 35 62181.5 36 89123.0 37 121800.5 38 153757.5 39 174504.5 40 180914.0 41 173865.0 42 162348.0 43 157923.0 44 151128.0 45 143760.0 46 144656.5 47 138320.0 48 118760.5 49 99407.0 50 84275.0 51 74326.5 52 67368.0 53 59409.0 54 51320.5 55 43891.5 56 36987.5 57 30648.5 58 25352.0 59 20977.0 60 17669.0 61 14870.5 62 12402.5 63 10427.5 64 8646.0 65 7120.5 66 5899.0 67 4950.5 68 4159.0 69 3441.0 70 2863.5 71 2391.0 72 1910.0 73 1491.5 74 1215.5 75 963.5 76 731.0 77 577.5 78 441.5 79 312.5 80 246.0 81 197.0 82 144.5 83 115.0 84 95.5 85 72.0 86 51.0 87 40.0 88 35.5 89 24.5 90 22.0 91 19.5 92 14.0 93 8.0 94 7.0 95 7.0 96 4.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28996620123428213 2 0.26858799604944905 3 0.23629100507599862 4 0.22537221928738138 5 0.2316973411519849 6 0.1803896487083854 7 0.14484175711178682 8 0.14314563504753558 9 0.12134339934663965 10-11 0.10558359849963869 12-13 0.12789820440744387 14-15 0.13065440276185208 16-17 0.12447062440260284 18-19 0.11141401809550226 20-21 0.11743878501122797 22-23 0.14708558525928583 24-25 0.5558510027438308 26-27 0.22268669268565028 28-29 0.19782790368146827 30-31 0.29729839557453486 32-33 0.3275989095348562 34-35 0.3356024855255416 36-37 0.3260617989141285 38-39 0.33335865737804266 40-41 0.3360971877942816 42-43 0.3471573170882531 44-45 0.3296837262388317 46-47 0.3477580269860088 48-49 0.3430053516184715 50-51 0.3494011452357521 52-53 0.3335530046979048 54-55 0.32590278747060497 56-57 0.3343480619155225 58-59 0.3268038523172384 60-61 0.30438323878041756 62-63 0.29199801412374976 64-65 0.3024044297054578 66-67 0.31710415426230176 68-69 0.30623837228819234 70-71 0.2863796097859176 72-73 0.2837647549368636 74-75 0.27524880873926894 76-77 0.2789590757548185 78-79 0.28685664411648826 80-81 0.29622065134620856 82-83 0.31805822292344305 84-85 0.30885322713724633 86-87 0.3107260285831904 88-89 0.316432772611869 90-91 0.3107083606450211 92-93 0.2930227545375682 94-95 0.2889944646349716 96-97 0.3051076242453582 98-99 0.3137825818864764 100-101 0.31583206271411335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2829985.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.97629923299988 #Duplication Level Percentage of deduplicated Percentage of total 1 92.05576266648924 55.211639678073766 2 3.9171435146203035 4.698715431429443 3 1.159559298201605 2.086382263420404 4 0.6097056079517832 1.4627154394621702 5 0.3915872882239164 1.1742978187178292 6 0.21356847049871353 0.7685427896018969 7 0.24723121995227135 1.0379609539317924 8 0.15580015368975766 0.747545331059542 9 0.11919671341056307 0.6434079975991853 >10 0.909672070257137 10.791726178584563 >50 0.11407501620620912 4.746774032118476 >100 0.09751357196444599 11.766377479987566 >500 0.007013272223322029 2.7189444313519484 >1k 0.00211185208908223 1.910724070053164 >5k 5.928422166034737E-5 0.23424610460819656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 6588 0.23279275331848048 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2878 0.10169665210239631 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0049823585637379705 0.0 0.0 2 0.005406389079800776 0.0 0.0 3 0.006148442482910687 0.0 0.0 4 0.006890495886020597 0.0 0.0 5 0.008197923310547582 0.0 0.0 6 0.009611358364090269 0.0 0.0 7 0.011448823933695762 0.0 0.0 8 0.013321625379639821 0.0 0.0 9 0.014947075691213911 0.0 0.0 10-11 0.024487762302627045 0.0 0.0 12-13 0.03416979241939445 0.0 0.0 14-15 0.043180440885729074 0.0 0.0 16-17 0.051449035948953795 0.0 0.0 18-19 0.060283005033595585 0.0 0.0 20-21 0.06805689782808036 0.0 0.0 22-23 0.07565411124087229 0.0 0.0 24-25 0.08291563382844785 0.0 0.0 26-27 0.09106055332448759 0.0 0.0 28-29 0.1001772094198379 0.0 1.766793816928358E-5 30-31 0.20314595307042263 0.0 3.533587633856716E-5 32-33 0.21664425783175528 0.0 3.533587633856716E-5 34-35 0.22662664289740053 0.0 3.533587633856716E-5 36-37 0.2370860622936164 0.0 3.533587633856716E-5 38-39 0.250955393756504 0.0 3.533587633856716E-5 40-41 0.266715194603505 0.0 3.533587633856716E-5 42-43 0.2840827778239107 0.0 3.533587633856716E-5 44-45 0.30503695249268103 0.0 3.533587633856716E-5 46-47 0.32795226829824187 0.0 7.067175267713433E-5 48-49 0.3535001068910259 0.0 7.067175267713433E-5 50-51 0.38063805991904553 0.0 7.067175267713433E-5 52-53 0.41277603944897234 0.0 7.067175267713433E-5 54-55 0.44793523640584665 0.0 7.067175267713433E-5 56-57 0.4850732424376808 3.533587633856716E-5 7.067175267713433E-5 58-59 0.5275646337348078 3.533587633856716E-5 7.067175267713433E-5 60-61 0.5726178760664808 3.533587633856716E-5 7.067175267713433E-5 62-63 0.6203566449998852 3.533587633856716E-5 8.833969084641791E-5 64-65 0.6738374938383065 3.533587633856716E-5 1.060076290157015E-4 66-67 0.7289261250501328 3.533587633856716E-5 1.2367556718498508E-4 68-69 0.7871419813179221 3.533587633856716E-5 1.4134350535426865E-4 70-71 0.8486440740851984 3.533587633856716E-5 1.4134350535426865E-4 72-73 0.9151991971688895 3.533587633856716E-5 1.4134350535426865E-4 74-75 0.9848815453085441 3.533587633856716E-5 1.4134350535426865E-4 76-77 1.0632388510893167 3.533587633856716E-5 1.4134350535426865E-4 78-79 1.1504124580165618 3.533587633856716E-5 1.4134350535426865E-4 80-81 1.2402185877310303 3.533587633856716E-5 1.4134350535426865E-4 82-83 1.3379752896216766 3.533587633856716E-5 1.5901144352355222E-4 84-85 1.4469334643116483 3.533587633856716E-5 1.7667938169283582E-4 86-87 1.561686722721145 3.533587633856716E-5 2.12015258031403E-4 88-89 1.6832421373258162 3.533587633856716E-5 2.12015258031403E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2050 0.0 84.86881 9 AAGCGGC 705 0.0 81.363976 9 AGAGCGG 2210 0.0 79.37221 8 CGGGAGA 3215 0.0 77.80847 4 GAGAGCG 2640 0.0 77.39983 7 CGGAAAG 1530 0.0 77.25118 5 TCGGAGA 795 0.0 75.23166 4 TCGGGAG 3420 0.0 74.39497 3 GTTCGGA 600 0.0 74.386986 2 ATCGGGA 3950 0.0 73.68579 2 TTCGGAG 595 0.0 73.396576 3 ATTCGGA 570 0.0 73.304085 2 ATCCGGA 720 0.0 69.902664 2 GGATCGG 1290 0.0 69.21069 1 GATCGGG 4320 0.0 69.146774 1 GATCCGG 785 0.0 68.361855 1 TCGGAAA 1140 0.0 68.28671 4 TCGGGAA 1040 0.0 66.63839 3 CCGGAAA 880 0.0 64.18913 4 ACCGGAA 410 0.0 63.677174 3 >>END_MODULE