FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34139-1.342000000356e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34139-1.342000000356e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2829985
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT65880.23279275331848048Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC28780.10169665210239631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC20500.084.868819
AAGCGGC7050.081.3639769
AGAGCGG22100.079.372218
CGGGAGA32150.077.808474
GAGAGCG26400.077.399837
CGGAAAG15300.077.251185
TCGGAGA7950.075.231664
TCGGGAG34200.074.394973
GTTCGGA6000.074.3869862
ATCGGGA39500.073.685792
TTCGGAG5950.073.3965763
ATTCGGA5700.073.3040852
ATCCGGA7200.069.9026642
GGATCGG12900.069.210691
GATCGGG43200.069.1467741
GATCCGG7850.068.3618551
TCGGAAA11400.068.286714
TCGGGAA10400.066.638393
CCGGAAA8800.064.189134
ACCGGAA4100.063.6771743
Produced by FastQC (version 0.11.2)