FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34098-1.34200000035704.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34098-1.34200000035704.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385241
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT39130.16405050894228299Illumina Single End PCR Primer 1 (96% over 32bp)
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA35970.15080237175195296No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC34340.14396868073289032No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG33940.1422917013417093No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG29960.12560575639945815No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC28820.12082636513459227No Hit
TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT27810.11659199217186021No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC27130.11374112720685246No Hit
GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT26270.11013562151581328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC14000.084.6813059
CGGGAGA20700.081.864044
AGAGCGG15600.080.25358
CGGAAAG11250.078.899685
ACCGGAA4650.076.565123
GAACCGG3300.076.2723541
AAGCGGC6250.075.874449
TCGGAGA4700.075.745824
GTTCGGA3700.074.428662
TCGGGAG21200.074.116483
ATTCGGA2750.072.515442
TCGGGAA8650.070.245173
GGATCGG8600.070.131081
GAGAGCG19700.069.811287
AACCGGA3550.068.211212
TCGGAAA8500.064.2205664
GGAAAGC9800.062.9259266
CGGAGAG11550.062.877555
CCGGAAA8000.061.7075924
AATCGGA3900.060.8722462
Produced by FastQC (version 0.11.2)