##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34098-1.34200000035704.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2385241 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.998923379230863 33.0 33.0 33.0 27.0 33.0 2 30.5844906237986 33.0 33.0 33.0 27.0 33.0 3 30.425143203558886 33.0 33.0 33.0 27.0 33.0 4 33.902170891746366 37.0 37.0 37.0 27.0 37.0 5 33.98347085263082 37.0 37.0 37.0 27.0 37.0 6 34.38289673873626 37.0 37.0 37.0 27.0 37.0 7 33.994946841849526 37.0 37.0 37.0 27.0 37.0 8 34.071543294786565 37.0 37.0 37.0 27.0 37.0 9 33.66024062138794 37.0 37.0 37.0 22.0 37.0 10-11 33.67690497522054 37.0 37.0 37.0 22.0 37.0 12-13 33.65618820068916 37.0 37.0 37.0 22.0 37.0 14-15 35.20188169665036 40.0 37.0 40.0 22.0 40.0 16-17 35.13012982755202 40.0 37.0 40.0 22.0 40.0 18-19 35.15557840905804 40.0 37.0 40.0 22.0 40.0 20-21 35.16189097873129 40.0 37.0 40.0 22.0 40.0 22-23 35.710525267677355 40.0 37.0 40.0 27.0 40.0 24-25 35.15205738120383 37.0 37.0 40.0 22.0 40.0 26-27 34.93780754229866 37.0 37.0 40.0 18.5 40.0 28-29 34.766987277176604 37.0 37.0 40.0 15.0 40.0 30-31 34.810260472631484 37.0 37.0 40.0 18.5 40.0 32-33 34.60931138614505 37.0 37.0 40.0 15.0 40.0 34-35 34.607624135255094 37.0 37.0 40.0 15.0 40.0 36-37 34.46615981362051 37.0 37.0 40.0 15.0 40.0 38-39 34.57071109376369 37.0 37.0 40.0 18.5 40.0 40-41 34.30626381149746 37.0 37.0 40.0 15.0 40.0 42-43 34.13250233414569 37.0 33.0 40.0 15.0 40.0 44-45 34.02700460875862 37.0 33.0 40.0 15.0 40.0 46-47 33.94529253018877 37.0 33.0 40.0 15.0 40.0 48-49 33.83788682149938 37.0 33.0 40.0 15.0 40.0 50-51 32.82664120732454 37.0 33.0 38.5 15.0 38.5 52-53 33.27209556602456 37.0 33.0 38.5 15.0 40.0 54-55 33.94796102364499 37.0 33.0 40.0 15.0 40.0 56-57 34.17579397637388 37.0 33.0 40.0 18.5 40.0 58-59 33.71735392775824 37.0 33.0 40.0 15.0 40.0 60-61 33.4760349163879 37.0 33.0 40.0 15.0 40.0 62-63 33.14190955966294 37.0 33.0 40.0 15.0 40.0 64-65 32.90917437692879 37.0 33.0 37.0 15.0 40.0 66-67 32.70357313998879 37.0 33.0 37.0 15.0 40.0 68-69 32.70106144410565 37.0 33.0 37.0 15.0 40.0 70-71 32.344939567951414 37.0 33.0 37.0 15.0 38.5 72-73 32.44222680223927 37.0 33.0 37.0 18.5 37.0 74-75 32.46900942085097 37.0 33.0 37.0 22.0 37.0 76-77 32.20523754203454 37.0 33.0 37.0 22.0 37.0 78-79 31.82331869190577 35.0 33.0 37.0 22.0 37.0 80-81 31.37599533967427 33.0 33.0 37.0 15.0 37.0 82-83 31.088198425232505 33.0 33.0 37.0 15.0 37.0 84-85 30.82141217596042 33.0 33.0 37.0 15.0 37.0 86-87 30.67339715357903 33.0 27.0 37.0 15.0 37.0 88-89 30.570616554050513 33.0 27.0 37.0 15.0 37.0 90-91 30.43835989738563 33.0 27.0 37.0 15.0 37.0 92-93 30.258091111128813 33.0 27.0 37.0 15.0 37.0 94-95 30.113670274827577 33.0 27.0 37.0 15.0 37.0 96-97 29.963148377878795 33.0 27.0 37.0 15.0 37.0 98-99 29.801607887840262 33.0 27.0 37.0 15.0 37.0 100-101 28.548931743165575 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.17835808718573176 2306 2 0.2729808942116705 2306 3 0.22968818427310111 2306 4 0.313781711125273 2306 5 0.2168723337536207 2306 6 0.030975986479880646 2306 7 0.44496608029534457 2306 8 0.13100919305276904 2306 9 0.3349536594220055 2306 10-11 0.2789546289263001 2306 12-13 0.28617043702616485 2306 14-15 0.5012257302366336 2306 16-17 0.5024959523903689 2306 18-19 0.36770662310902225 2306 20-21 0.4325096043600425 2306 22-23 0.3466299067120602 2306 24-25 0.5594506234137953 2306 26-27 0.2284695350185899 2306 28-29 0.34140995168671395 2306 30-31 0.23237523280975125 2306 32-33 0.41747715455854006 2306 34-35 0.32889565958128486 2306 36-37 0.358780885634566 2306 38-39 0.18543826564314259 2306 40-41 0.4465312970966835 2306 42-43 0.3713098196286424 2306 44-45 0.6021192837352984 2306 46-47 0.48911028228332043 2306 48-49 0.5618488969357003 2306 50-51 0.4752385039705658 2306 52-53 0.4072523304331028 2306 54-55 0.41240490928321094 2306 56-57 0.23140434104483631 2306 58-59 0.4328919410381431 2306 60-61 0.5153102523240349 2306 62-63 0.4717818633215032 2306 64-65 0.3486444164192335 2306 66-67 0.4718000522444541 2306 68-69 0.25294112204029773 2306 70-71 0.38636304058607607 2306 72-73 0.16896316469902217 2306 74-75 0.26546473511840674 2306 76-77 0.4422870978846376 2306 78-79 0.4135107452272102 2306 80-81 0.5894446652100065 2306 82-83 0.516496907396661 2306 84-85 0.46627113761607575 2306 86-87 0.4969541793188128 2306 88-89 0.3474619953014013 2306 90-91 0.33702953173101236 2306 92-93 0.3346386718184924 2306 94-95 0.2682965386352727 2306 96-97 0.3841414024405765 2306 98-99 0.42989333315967215 2306 100-101 0.38317321694241 2307 1 0.0863132031079843 2307 2 0.17830575226966516 2307 3 0.012017847972479956 2307 4 0.0888928960847224 2307 5 0.05943189754562184 2307 6 -0.08411871223068346 2307 7 0.4041723363495322 2307 8 -0.1328868646243535 2307 9 0.2412557313392938 2307 10-11 0.17547498749925694 2307 12-13 0.2321081498802542 2307 14-15 0.4489823512508835 2307 16-17 0.41158103628286824 2307 18-19 0.3571340352982446 2307 20-21 0.22795549906016532 2307 22-23 0.2521341808810931 2307 24-25 0.4152622323726973 2307 26-27 -0.07569424449226858 2307 28-29 0.20378925089740108 2307 30-31 0.1525355053101194 2307 32-33 0.20028349357426833 2307 34-35 -0.014498527925930205 2307 36-37 0.20034276032964726 2307 38-39 -0.04174819512807204 2307 40-41 0.16727674894246292 2307 42-43 0.24030556667136693 2307 44-45 0.39633828452012665 2307 46-47 0.2719771631887369 2307 48-49 0.03137688231372948 2307 50-51 -0.091856640186883 2307 52-53 -0.08903900008613874 2307 54-55 0.179702507876641 2307 56-57 0.027750409681367216 2307 58-59 0.20515132311609108 2307 60-61 0.19273997070261117 2307 62-63 0.2443408614666538 2307 64-65 0.14245258671665084 2307 66-67 0.18913571415733088 2307 68-69 0.04087289913773162 2307 70-71 0.176064968263411 2307 72-73 -0.0912500016742186 2307 74-75 -0.18091238517346397 2307 76-77 0.020669842827402363 2307 78-79 0.33009477442401547 2307 80-81 0.46898604345570405 2307 82-83 0.3819367390695838 2307 84-85 0.36404505210440874 2307 86-87 0.2513494321276042 2307 88-89 0.2226900216888268 2307 90-91 0.006298468527045742 2307 92-93 0.19934566674888288 2307 94-95 0.2479204365077301 2307 96-97 0.1799294840257062 2307 98-99 0.03639785951292396 2307 100-101 0.03770911548421907 2201 1 0.1755101034171993 2201 2 0.20911451834748362 2201 3 0.1503374543356557 2201 4 0.23244339665246372 2201 5 0.22410048030697283 2201 6 0.08519586521399702 2201 7 0.4368503461196198 2201 8 0.07612036665940991 2201 9 0.3243626489796867 2201 10-11 0.2597100639844143 2201 12-13 0.2618638754766138 2201 14-15 0.5802698827646253 2201 16-17 0.5371887618261013 2201 18-19 0.5842236261895266 2201 20-21 0.4804054920878613 2201 22-23 0.4006613076023129 2201 24-25 0.6160966410652904 2201 26-27 0.2776745720075411 2201 28-29 0.36344302579575327 2201 30-31 0.2272910040296523 2201 32-33 0.5095027716854119 2201 34-35 0.24232216412585927 2201 36-37 0.33850811811993253 2201 38-39 0.21787387235062994 2201 40-41 0.40597938978870474 2201 42-43 0.4082426974431641 2201 44-45 0.41353679288801004 2201 46-47 0.5292608776382366 2201 48-49 0.34171767897232996 2201 50-51 0.3381358120085096 2201 52-53 0.4223071929207478 2201 54-55 0.5337410260278901 2201 56-57 0.4527472832080548 2201 58-59 0.6560889330960222 2201 60-61 0.502489227542668 2201 62-63 0.3534506300080196 2201 64-65 0.41872602973293027 2201 66-67 0.29992907474539265 2201 68-69 0.33761879377392034 2201 70-71 0.3717069411400118 2201 72-73 0.17685615233198604 2201 74-75 0.3182306014551912 2201 76-77 0.19446025733689964 2201 78-79 0.5506227831980617 2201 80-81 0.575668506651855 2201 82-83 0.49427521383756456 2201 84-85 0.5468949072352558 2201 86-87 0.4895869754832134 2201 88-89 0.33723321350266744 2201 90-91 0.312215028764502 2201 92-93 0.4410892870832406 2201 94-95 0.5462956940959351 2201 96-97 0.5038228508113818 2201 98-99 0.32540268922249993 2201 100-101 0.2225351507619031 1101 1 0.03825757209804337 1101 2 -0.10636638333092208 1101 3 -0.01584111872497118 1101 4 0.1355104872185322 1101 5 -0.07422241874709101 1101 6 0.12799542912858186 1101 7 0.23769979872797364 1101 8 0.0728408476590161 1101 9 0.19474109017016872 1101 10-11 0.23044768810228788 1101 12-13 0.14713123892246927 1101 14-15 0.31704232843348024 1101 16-17 0.15651217660992245 1101 18-19 0.1830092458159811 1101 20-21 0.21133817956215495 1101 22-23 0.3004943156124469 1101 24-25 0.31492730544272973 1101 26-27 0.16715616567778113 1101 28-29 0.13955716527780737 1101 30-31 0.17770378368248174 1101 32-33 0.12796589700774064 1101 34-35 -0.059468919624741545 1101 36-37 0.1724621985868353 1101 38-39 0.1282379514466001 1101 40-41 0.10022345400032151 1101 42-43 -4.0706982561999894E-4 1101 44-45 0.11912051410669733 1101 46-47 0.18387195864698214 1101 48-49 0.10746356129455137 1101 50-51 0.06614738002281939 1101 52-53 0.08050044143067936 1101 54-55 0.3257741110710697 1101 56-57 0.3892602972241548 1101 58-59 0.32339166646373485 1101 60-61 0.30197219214010573 1101 62-63 0.230170980443134 1101 64-65 0.22090697906738654 1101 66-67 0.38490580162405763 1101 68-69 0.2845577744568999 1101 70-71 0.33929417454363886 1101 72-73 0.09512447542688562 1101 74-75 0.2103486839850035 1101 76-77 0.1301026672963559 1101 78-79 0.24084598320328254 1101 80-81 0.34919302992824086 1101 82-83 0.32676710251827146 1101 84-85 0.3178211970492697 1101 86-87 0.31016211208631717 1101 88-89 0.24443528164427875 1101 90-91 -0.051658558690860445 1101 92-93 0.12525781973416272 1101 94-95 0.2398057247133032 1101 96-97 0.04301974859896518 1101 98-99 0.13902213288718102 1101 100-101 0.029495935715651456 2304 1 0.23178548175352276 2304 2 0.2825601965097668 2304 3 0.20701682512052244 2304 4 0.2378857485192114 2304 5 0.21621108137175327 2304 6 0.2050939707203412 2304 7 0.47035912358185783 2304 8 0.14737922153077676 2304 9 0.3046981241103097 2304 10-11 0.177681222350202 2304 12-13 0.2689871289293606 2304 14-15 0.1770752386069816 2304 16-17 0.19296360667885182 2304 18-19 0.24947292578282543 2304 20-21 0.26933321865822535 2304 22-23 -0.00802795665220657 2304 24-25 0.2623719523592243 2304 26-27 0.01982682625809673 2304 28-29 0.21125916975114478 2304 30-31 0.3608169642069683 2304 32-33 0.3582981069259006 2304 34-35 0.14279079040006337 2304 36-37 0.25155290268170916 2304 38-39 0.1573930741588896 2304 40-41 0.15714586521902874 2304 42-43 0.2364873068008464 2304 44-45 0.1832078357670781 2304 46-47 0.05993078253489159 2304 48-49 0.28025135771466125 2304 50-51 0.26137650186968386 2304 52-53 0.17659449139319605 2304 54-55 0.34922833850475143 2304 56-57 0.3971465499133373 2304 58-59 0.26937144499045473 2304 60-61 0.28827912216942764 2304 62-63 0.16612290721189993 2304 64-65 0.16812978703651993 2304 66-67 0.28504926560265176 2304 68-69 0.2376511479325245 2304 70-71 0.24244599404961775 2304 72-73 0.15838709251701744 2304 74-75 0.21142301831580568 2304 76-77 0.2400834696951364 2304 78-79 0.30526801351721033 2304 80-81 0.15967199484233774 2304 82-83 0.09301089377026273 2304 84-85 0.24211451201791334 2304 86-87 0.30780440287817257 2304 88-89 0.31786665479079446 2304 90-91 0.2505108311910895 2304 92-93 0.24038760161284145 2304 94-95 0.14119965339574136 2304 96-97 0.053334893982707854 2304 98-99 0.28581540214725365 2304 100-101 0.20517787717210823 2202 1 0.02438656997847133 2202 2 -0.1247109855262778 2202 3 -0.17108617206951493 2202 4 -0.11162240641209564 2202 5 -0.11886487413542568 2202 6 0.047976483230961264 2202 7 0.020688514077704667 2202 8 -0.050762662068521536 2202 9 -0.10900593473184728 2202 10-11 -0.09310987782350821 2202 12-13 -0.1345254614126432 2202 14-15 0.009454614848081633 2202 16-17 -0.014766789891361043 2202 18-19 0.06890840627588801 2202 20-21 0.06461996426570948 2202 22-23 0.075953220776384 2202 24-25 0.08964957042478972 2202 26-27 0.11431451785833247 2202 28-29 0.05830010497064109 2202 30-31 0.2527883889565459 2202 32-33 0.011548684659757669 2202 34-35 -0.06677954945742925 2202 36-37 -0.16548311881615518 2202 38-39 0.029611765847853633 2202 40-41 0.09164451473376545 2202 42-43 -0.1267468408170842 2202 44-45 0.04644322194911865 2202 46-47 0.1555084105540061 2202 48-49 0.21410978218003152 2202 50-51 -0.03929056624188121 2202 52-53 -0.009808731936125525 2202 54-55 0.15449644710025012 2202 56-57 0.4708460688550389 2202 58-59 0.2650193558931093 2202 60-61 0.008268150743319325 2202 62-63 0.18486778687525884 2202 64-65 0.17682248036398818 2202 66-67 0.15201667064869184 2202 68-69 0.2863012706854846 2202 70-71 0.26291461716716924 2202 72-73 0.22236737367557424 2202 74-75 0.40174854899547796 2202 76-77 0.30763531936678135 2202 78-79 0.17485588243479455 2202 80-81 0.22259938390865486 2202 82-83 0.20711203626683883 2202 84-85 0.21270154186460388 2202 86-87 0.01566980293662823 2202 88-89 -0.006778676745792467 2202 90-91 0.08658338042176084 2202 92-93 0.12759325685154366 2202 94-95 0.20720386091062792 2202 96-97 0.25569194904656456 2202 98-99 0.10428198771424135 2202 100-101 0.2178742852911455 1102 1 -0.06989597770933287 1102 2 -0.14833712264481136 1102 3 -0.10936868247474152 1102 4 -0.21146048141108054 1102 5 -0.11293779207308319 1102 6 -0.06450801060724842 1102 7 0.011381004011411733 1102 8 0.09608153744070336 1102 9 -0.03183820720645514 1102 10-11 -0.10260083510446094 1102 12-13 -0.09424121567301569 1102 14-15 -0.0838897253181301 1102 16-17 -0.062353971803212005 1102 18-19 -0.17367278500405803 1102 20-21 -0.25141234786400446 1102 22-23 -0.04932131454716426 1102 24-25 -0.11962747808323115 1102 26-27 -0.06507736945339104 1102 28-29 -0.14736943075962472 1102 30-31 -0.0492784214175046 1102 32-33 -0.03593808849950619 1102 34-35 -0.005470685355795979 1102 36-37 -0.056281609888678474 1102 38-39 -0.13556719898451064 1102 40-41 -0.1579860488431919 1102 42-43 -0.17295075447063368 1102 44-45 -0.2190682356639826 1102 46-47 -0.12213301750418282 1102 48-49 -0.1191739822754343 1102 50-51 -0.1959970659504009 1102 52-53 -0.04486875688156289 1102 54-55 0.08119318555282007 1102 56-57 0.21600653644630796 1102 58-59 0.07212355052170238 1102 60-61 0.04137952565689318 1102 62-63 0.10355258473592244 1102 64-65 0.09521410161369914 1102 66-67 0.22566013569120003 1102 68-69 0.155949950204473 1102 70-71 0.08633565775771501 1102 72-73 0.15228015787047156 1102 74-75 0.23130596246601698 1102 76-77 0.3453436781200594 1102 78-79 0.3152832424217742 1102 80-81 0.06258151369268461 1102 82-83 0.1630633096362324 1102 84-85 0.07000990095680848 1102 86-87 0.15421629939139692 1102 88-89 0.016993413179775985 1102 90-91 0.023920692822624545 1102 92-93 0.024097252865697527 1102 94-95 0.048265067035817566 1102 96-97 0.006487804918911877 1102 98-99 0.1061704772276677 1102 100-101 0.2126543011531865 2305 1 0.03269671944858388 2305 2 -0.23488191842873718 2305 3 -0.26849605872168425 2305 4 -0.12010790283024164 2305 5 -0.16740560686698558 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0.11701757037956995 1213 100-101 0.14881634894608453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1113.0 3 350.0 4 370.0 5 302.0 6 828.0 7 4226.0 8 24397.0 9 54528.0 10 53425.0 11 29621.0 12 15355.0 13 9878.0 14 7580.0 15 6352.0 16 6067.0 17 6101.0 18 6483.0 19 7042.0 20 7814.0 21 8784.0 22 10070.0 23 11062.0 24 12778.0 25 14861.0 26 17793.0 27 21859.0 28 26923.0 29 32956.0 30 40737.0 31 52734.0 32 71950.0 33 102377.0 34 156537.0 35 263696.0 36 486866.0 37 675417.0 38 135917.0 39 92.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.187711265577256 23.580423567640935 22.19350802089119 14.038357145890615 2 33.26503510408473 24.23588027995828 29.896043481057333 12.603041134899653 3 36.80705126777327 20.330348516297843 27.10390937206213 15.758690843866766 4 30.232086136308535 17.91211982033505 32.69065028744459 19.165143755911828 5 33.58404679088035 16.086046068913003 32.36565334893253 17.964253791274114 6 31.113151447516472 16.476239157854415 36.16189201346902 16.248717381160088 7 36.63851722283267 17.822070266822802 27.88361052411037 17.655801986234163 8 30.06925536314456 29.337613072658304 23.20325472916094 17.3898768350362 9 31.127569708606146 27.17344856217211 23.464093550951738 18.234888178270005 10-11 26.636489739215108 29.09704253519767 27.646493453621336 16.619974271965887 12-13 28.60664630804626 24.618307351919164 27.495451004871924 19.279595335162654 14-15 26.493812410108035 24.650465612046215 28.821977593863373 20.033744383982373 16-17 27.118358403025784 25.48582870611842 29.568454270593165 17.827358620262636 18-19 26.707731581439237 25.649510091971084 29.632426270352674 18.01033205623701 20-21 25.562568931339925 27.33724333405741 28.986033762845757 18.114153971756906 22-23 27.69064690656774 25.504900101170016 28.422224316955436 18.382228675306813 24-25 25.23182243730459 27.584672345465233 28.62596811836726 18.557537098862912 26-27 22.62770226164622 29.847431479256425 28.983796041945787 18.541070217151567 28-29 23.453556278654766 29.102635839451814 28.349913925428922 19.0938939564645 30-31 25.560413665389202 27.394966852441012 28.0159947589601 19.028624723209685 32-33 23.649198165974628 26.947242852308634 29.429457092399723 19.97410188931702 34-35 21.105160882282853 26.973024577663107 33.06674036621355 18.85507417384049 36-37 21.41290051487484 27.70326115439473 30.625610154655934 20.258228176074493 38-39 20.67437476430556 27.320153444046536 28.213610198362964 23.791861593284942 40-41 21.869892912324566 26.529071251983943 29.065678225449908 22.535357610241583 42-43 24.076888934699518 26.685374231941555 30.21643418940779 19.021302643951138 44-45 22.42599915157794 28.10365471367438 30.73784326233214 18.732502872415534 46-47 23.61006545706669 28.751570058750858 29.079139375359052 18.559225108823398 48-49 21.22541266628285 28.99476927225636 30.219121783802898 19.56069627765789 50-51 19.935924405102487 27.71658644946316 31.002364444145265 21.345124701289084 52-53 23.791361418255402 26.997445136262556 28.563384997794707 20.64780844768734 54-55 25.976923889045217 26.8972006408582 28.15828558233089 18.96758988776569 56-57 26.613247384615256 26.837941529884656 27.199622754451973 19.34918833104811 58-59 25.08747887951322 26.80014688123441 27.729237988400087 20.383136250852285 60-61 21.79855006306862 27.1015916757413 27.518586791944628 23.581271469245454 62-63 23.449968790083297 27.20001530536838 27.535471978246196 21.81454392630213 64-65 22.00891707290406 28.100848499120595 29.35983574587709 20.530398682098255 66-67 21.097383766778613 28.022862862778663 28.680043992925803 22.199709377516925 68-69 21.044971228145133 26.983031260572478 32.21015828252412 19.76183922875827 70-71 20.398969046157468 30.449381511785333 30.223618597532777 18.928030844524425 72-73 20.167546090941578 33.7504467195022 27.1732641005697 18.908743088986522 74-75 20.86411054318955 33.40691836643749 26.597437037921445 19.131534052451514 76-77 20.85316395507154 32.759125554453355 26.844318767298915 19.54339172317619 78-79 20.72074912971462 32.61604032818047 27.344714578559877 19.318495963545036 80-81 19.846318581983223 32.70096942329312 27.51560918884359 19.937102805880073 82-83 19.183718875241887 32.26647415156737 27.915726837729203 20.63408013546154 84-85 19.360814549418393 31.6971244455874 28.168565402130362 20.773495602863843 86-87 19.381772501970296 31.6658283616801 28.916832855723783 20.03556628062582 88-89 19.766218030096915 31.811818678290294 28.72896772933174 19.69299556228105 90-91 20.50785365195131 31.081780679793308 27.917294520043857 20.493071148211527 92-93 19.983849438588237 31.770122134153095 27.60665372855746 20.639374698701207 94-95 19.758217865868183 31.806413676432655 27.853571788216108 20.581796669483058 96-97 20.22658291061056 32.3821378820932 27.28513680447867 20.106142402817568 98-99 20.64476256585288 31.50642615572285 27.175576339500036 20.67323493892424 100-101 20.733317267677794 31.50144906325032 26.756403025894727 21.008830643177166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 648.0 1 491.5 2 254.5 3 175.0 4 177.5 5 206.5 6 290.5 7 430.0 8 541.0 9 645.5 10 776.5 11 843.0 12 807.5 13 719.0 14 618.0 15 547.0 16 466.0 17 424.5 18 435.0 19 439.0 20 488.0 21 546.5 22 644.5 23 802.5 24 1010.0 25 1308.5 26 1800.0 27 2573.0 28 3396.0 29 4229.0 30 5391.0 31 6879.5 32 11010.5 33 19951.5 34 34592.5 35 55051.5 36 83861.0 37 119479.0 38 149123.5 39 165078.5 40 168551.5 41 155727.5 42 143964.5 43 146235.0 44 142813.0 45 132901.5 46 127562.5 47 115069.5 48 93827.0 49 78681.5 50 68644.5 51 58818.0 52 49182.5 53 42321.0 54 35912.5 55 28256.5 56 22512.0 57 18587.0 58 15544.0 59 12905.5 60 10446.5 61 8403.5 62 6693.0 63 5263.5 64 4294.5 65 3438.0 66 2643.5 67 2132.0 68 1749.0 69 1326.0 70 965.5 71 751.0 72 554.5 73 416.0 74 310.5 75 223.5 76 168.5 77 120.0 78 109.0 79 86.5 80 57.5 81 53.0 82 40.0 83 28.5 84 22.5 85 22.0 86 16.0 87 13.0 88 12.5 89 7.5 90 4.0 91 4.5 92 4.5 93 3.0 94 3.0 95 3.0 96 3.5 97 2.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29405833624359135 2 0.2707483227061752 3 0.24123348542138928 4 0.2296623276222403 5 0.2361186982782872 6 0.1832099984865261 7 0.1487480719977562 8 0.1457295090936304 9 0.12438994634085193 10-11 0.11009369703103375 12-13 0.1308672792392886 14-15 0.13478721856617423 16-17 0.12780679185038327 18-19 0.11541810659803349 20-21 0.12109887428565919 22-23 0.15120065435735844 24-25 0.5633183397400934 26-27 0.2246942761758665 28-29 0.19901552924840715 30-31 0.29711882363249664 32-33 0.32900239430732575 34-35 0.33629725465896315 36-37 0.3257532467369125 38-39 0.33361408763307354 40-41 0.3366536127795891 42-43 0.3482247705787382 44-45 0.33063744921372723 46-47 0.34962924081885227 48-49 0.3450175474931045 50-51 0.35053061724161205 52-53 0.33583608532638837 54-55 0.3271786792194164 56-57 0.3357941608416089 58-59 0.3274302261280935 60-61 0.30502158901343723 62-63 0.29307311085127247 64-65 0.30263189338100427 66-67 0.31803914153747986 68-69 0.30644702149594105 70-71 0.28764389007232394 72-73 0.28470917613775715 74-75 0.27615658124273396 76-77 0.2791961063892495 78-79 0.287371380921257 80-81 0.2965947675727526 82-83 0.318290688446157 84-85 0.30967520682396454 86-87 0.31137314845753533 88-89 0.31676044475170434 90-91 0.3114989219118739 92-93 0.2932827332751701 94-95 0.2893418317058947 96-97 0.3061954745872639 98-99 0.31432882463449185 100-101 0.31434978687688164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2385241.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.309294250424784 #Duplication Level Percentage of deduplicated Percentage of total 1 87.68727294351626 37.09986632984541 2 5.049639466365698 4.272933640820486 3 1.899442192002608 2.4109217583933056 4 1.1585313609192553 1.960665769899114 5 0.7695851015780181 1.6280301256703706 6 0.4369481970205858 1.1092181903961928 7 0.3531826039060948 1.0460034698955933 8 0.270931772642031 0.9170345672400702 9 0.18384510869802062 0.7000521120365295 >10 1.6919006154440308 14.327976670234849 >50 0.24062467042554447 7.053473017118204 >100 0.2388318418035954 20.110669421098 >500 0.01466735567109035 4.051225315201061 >1k 0.00459677000728854 3.3119296121506427 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT 3913 0.16405050894228299 Illumina Single End PCR Primer 1 (96% over 32bp) TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 3597 0.15080237175195296 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3434 0.14396868073289032 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3394 0.1422917013417093 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 2996 0.12560575639945815 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 2882 0.12082636513459227 No Hit TGTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGT 2781 0.11659199217186021 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 2713 0.11374112720685246 No Hit GTGTTTGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT 2627 0.11013562151581328 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004737466780086373 0.0 0.0 2 0.004989013688763525 0.0 0.0 3 0.0055759564756768815 0.0 0.0 4 0.006079050293031186 0.0 0.0 5 0.007085237927739797 0.0 0.0 6 0.008468745925464136 0.0 0.0 7 0.009642631499290847 0.0 0.0 8 0.01127768640569234 0.0 0.0 9 0.013122363735991457 0.0 0.0 10-11 0.020731657723475322 0.0 0.0 12-13 0.027481499772978916 0.0 0.0 14-15 0.0347973223670061 0.0 0.0 16-17 0.04131657975022231 0.0 0.0 18-19 0.04750044125520231 0.0 0.0 20-21 0.0540825853655878 0.0 0.0 22-23 0.06068569171836305 0.0 0.0 24-25 0.06774996740371309 0.0 0.0 26-27 0.07475135636189384 0.0 0.0 28-29 0.08315721556018868 0.0 0.0 30-31 0.14210304116020142 0.0 0.0 32-33 0.15396767035280712 0.0 0.0 34-35 0.16329586821625153 0.0 0.0 36-37 0.1753491575903651 0.0 0.0 38-39 0.18857633253830536 0.0 0.0 40-41 0.20331278893830854 0.0 0.0 42-43 0.2204599032131344 0.0 0.0 44-45 0.24052076918013737 4.192448477952542E-5 0.0 46-47 0.2630971042339118 4.192448477952542E-5 0.0 48-49 0.28674251364956416 4.192448477952542E-5 0.0 50-51 0.3130082033639368 4.192448477952542E-5 0.0 52-53 0.34216668252809673 4.192448477952542E-5 0.0 54-55 0.37335849920406367 4.192448477952542E-5 0.0 56-57 0.4087008398732036 4.192448477952542E-5 0.0 58-59 0.4448607079955443 4.192448477952542E-5 0.0 60-61 0.48263466878189665 4.192448477952542E-5 0.0 62-63 0.5245381913190323 4.192448477952542E-5 0.0 64-65 0.5700052950624277 4.192448477952542E-5 2.096224238976271E-5 66-67 0.6179669056502047 4.192448477952542E-5 4.192448477952542E-5 68-69 0.6674587599324345 4.192448477952542E-5 4.192448477952542E-5 70-71 0.7199901393611798 4.192448477952542E-5 4.192448477952542E-5 72-73 0.7773637967819604 4.192448477952542E-5 4.192448477952542E-5 74-75 0.8377979415916463 4.192448477952542E-5 4.192448477952542E-5 76-77 0.9017537431228123 4.192448477952542E-5 4.192448477952542E-5 78-79 0.9718724439165686 4.192448477952542E-5 4.192448477952542E-5 80-81 1.0472317053077655 4.192448477952542E-5 4.192448477952542E-5 82-83 1.1251483602705137 4.192448477952542E-5 4.192448477952542E-5 84-85 1.2104646867968478 4.192448477952542E-5 4.192448477952542E-5 86-87 1.3037885899160715 4.192448477952542E-5 6.288672716928813E-5 88-89 1.4065035776259087 4.192448477952542E-5 8.384896955905084E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1400 0.0 84.681305 9 CGGGAGA 2070 0.0 81.86404 4 AGAGCGG 1560 0.0 80.2535 8 CGGAAAG 1125 0.0 78.89968 5 ACCGGAA 465 0.0 76.56512 3 GAACCGG 330 0.0 76.272354 1 AAGCGGC 625 0.0 75.87444 9 TCGGAGA 470 0.0 75.74582 4 GTTCGGA 370 0.0 74.42866 2 TCGGGAG 2120 0.0 74.11648 3 ATTCGGA 275 0.0 72.51544 2 TCGGGAA 865 0.0 70.24517 3 GGATCGG 860 0.0 70.13108 1 GAGAGCG 1970 0.0 69.81128 7 AACCGGA 355 0.0 68.21121 2 TCGGAAA 850 0.0 64.220566 4 GGAAAGC 980 0.0 62.925926 6 CGGAGAG 1155 0.0 62.87755 5 CCGGAAA 800 0.0 61.707592 4 AATCGGA 390 0.0 60.872246 2 >>END_MODULE