##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34042-1.34200000035847.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5509837 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86269503072414 33.0 33.0 33.0 22.0 33.0 2 30.296358857802872 33.0 33.0 33.0 22.0 33.0 3 30.134501256570747 33.0 33.0 33.0 15.0 33.0 4 33.47335138952386 37.0 37.0 37.0 15.0 37.0 5 33.56108683432922 37.0 37.0 37.0 15.0 37.0 6 34.163826806491734 37.0 37.0 37.0 27.0 37.0 7 33.65825304813917 37.0 37.0 37.0 22.0 37.0 8 33.834672786145944 37.0 37.0 37.0 22.0 37.0 9 33.24719351951791 37.0 37.0 37.0 15.0 37.0 10-11 33.31984612248965 37.0 37.0 37.0 15.0 37.0 12-13 33.29231890162994 37.0 37.0 37.0 15.0 37.0 14-15 34.877128216315654 40.0 37.0 40.0 15.0 40.0 16-17 34.80243371990859 40.0 37.0 40.0 15.0 40.0 18-19 34.866111737969746 40.0 37.0 40.0 15.0 40.0 20-21 34.83221690587217 40.0 37.0 40.0 15.0 40.0 22-23 35.66366364377022 40.0 37.0 40.0 24.5 40.0 24-25 34.97853511818953 40.0 37.0 40.0 18.5 40.0 26-27 34.75604586850754 40.0 37.0 40.0 18.5 40.0 28-29 34.515456264858656 40.0 37.0 40.0 15.0 40.0 30-31 34.52209684242928 40.0 37.0 40.0 15.0 40.0 32-33 34.288228762484266 38.5 37.0 40.0 15.0 40.0 34-35 34.48617563459682 37.0 37.0 40.0 15.0 40.0 36-37 34.31933013626356 37.0 37.0 40.0 15.0 40.0 38-39 34.18778994732512 37.0 37.0 40.0 15.0 40.0 40-41 34.01214591284642 37.0 37.0 40.0 15.0 40.0 42-43 33.918770646028186 37.0 37.0 40.0 15.0 40.0 44-45 33.78524192276468 37.0 33.0 40.0 15.0 40.0 46-47 33.69488843317869 37.0 33.0 40.0 15.0 40.0 48-49 33.592181402099555 37.0 33.0 40.0 10.5 40.0 50-51 32.650618611766554 37.0 33.0 38.5 15.0 38.5 52-53 33.050829180609156 37.0 33.0 38.5 15.0 40.0 54-55 33.69036915248128 37.0 33.0 40.0 15.0 40.0 56-57 34.03302193876153 37.0 33.0 40.0 18.5 40.0 58-59 33.46607930506837 37.0 33.0 40.0 15.0 40.0 60-61 33.2033129110716 37.0 33.0 40.0 15.0 40.0 62-63 32.81276587674009 37.0 33.0 40.0 6.0 40.0 64-65 32.5969476048021 37.0 33.0 37.0 6.0 40.0 66-67 32.40388499696089 37.0 33.0 37.0 10.5 40.0 68-69 32.488568627347775 37.0 33.0 37.0 15.0 40.0 70-71 32.11147670974658 37.0 33.0 37.0 15.0 38.5 72-73 32.35993297079388 37.0 33.0 37.0 18.5 37.0 74-75 32.525587689799174 37.0 33.0 37.0 22.0 37.0 76-77 32.29397140060586 37.0 33.0 37.0 22.0 37.0 78-79 31.892147444652174 37.0 33.0 37.0 22.0 37.0 80-81 31.369877094367766 35.0 33.0 37.0 15.0 37.0 82-83 31.021455262651145 33.0 33.0 37.0 15.0 37.0 84-85 30.729940831280487 33.0 33.0 37.0 15.0 37.0 86-87 30.573648185962668 33.0 33.0 37.0 15.0 37.0 88-89 30.469995028891056 33.0 27.0 37.0 15.0 37.0 90-91 30.33715734240414 33.0 27.0 37.0 15.0 37.0 92-93 30.167391521745564 33.0 27.0 37.0 15.0 37.0 94-95 30.034187581229716 33.0 27.0 37.0 10.5 37.0 96-97 29.898182741159133 33.0 27.0 37.0 6.0 37.0 98-99 29.75736224138754 33.0 27.0 37.0 6.0 37.0 100-101 28.555138382496615 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.18677499128862607 2306 2 0.002303360476961558 2306 3 -0.15029728094408767 2306 4 0.03387201432802556 2306 5 -0.043121532924864425 2306 6 -0.31868964174820746 2306 7 0.13237761816454707 2306 8 -0.27568043786632757 2306 9 0.1251775922920757 2306 10-11 0.01753879036886019 2306 12-13 0.016500228526368232 2306 14-15 0.31282593536727177 2306 16-17 0.20225315336205796 2306 18-19 0.27066532277907385 2306 20-21 0.27626995008441924 2306 22-23 0.05994880349822296 2306 24-25 0.3534521500698773 2306 26-27 -0.024515614707794953 2306 28-29 0.04975874839384886 2306 30-31 0.0488887304384491 2306 32-33 0.16357363030064676 2306 34-35 -0.06362291585305258 2306 36-37 0.2505530346924729 2306 38-39 0.029544885231111095 2306 40-41 0.09193821883690845 2306 42-43 0.17899409761385954 2306 44-45 0.2321956601121613 2306 46-47 0.1371655034698236 2306 48-49 0.15526610936831275 2306 50-51 0.09610883304791429 2306 52-53 0.07389194324818504 2306 54-55 0.1515717176822875 2306 56-57 0.048763606909624 2306 58-59 0.31636121815615326 2306 60-61 0.22799298644383015 2306 62-63 0.15755890718312315 2306 64-65 0.16038891201076666 2306 66-67 0.12814377677302247 2306 68-69 -0.1058456724772796 2306 70-71 0.1102619257492492 2306 72-73 -0.17708932716774228 2306 74-75 -0.13694174656922797 2306 76-77 -0.018678131848538726 2306 78-79 0.1189026980484087 2306 80-81 0.3202908255433492 2306 82-83 0.33129674625864425 2306 84-85 0.29407440418361475 2306 86-87 0.34621372391382366 2306 88-89 0.358211069963442 2306 90-91 0.2145445277957343 2306 92-93 0.1924909231427634 2306 94-95 0.1356827599054249 2306 96-97 0.19096776776150293 2306 98-99 0.2010779383435235 2306 100-101 0.20092414691816884 2307 1 0.0667558069833376 2307 2 0.17492529502176524 2307 3 0.10576937333717851 2307 4 0.31911793303705593 2307 5 0.20977574661637277 2307 6 -3.515488039909087E-4 2307 7 0.33626638740096837 2307 8 -0.05830500841899777 2307 9 0.37709458928176076 2307 10-11 0.248073365640046 2307 12-13 0.284547976986552 2307 14-15 0.5369626427906695 2307 16-17 0.5129838837883298 2307 18-19 0.44324225064069367 2307 20-21 0.42112221307800013 2307 22-23 0.2375392155539302 2307 24-25 0.49451659032374806 2307 26-27 0.1332874755045026 2307 28-29 0.2984843810768396 2307 30-31 0.2992244652242704 2307 32-33 0.4703443206332878 2307 34-35 0.20577455357939556 2307 36-37 0.49010093468201177 2307 38-39 0.2880340910255015 2307 40-41 0.3218174228173325 2307 42-43 0.30522791096367996 2307 44-45 0.40525286477105027 2307 46-47 0.382681207445593 2307 48-49 0.29864178323550306 2307 50-51 0.11764709982615784 2307 52-53 0.17779963310240987 2307 54-55 0.2522918794158926 2307 56-57 0.009347137395522509 2307 58-59 0.38979602683185277 2307 60-61 0.19899642037840692 2307 62-63 0.3236388428886485 2307 64-65 0.39365355021184456 2307 66-67 0.3299260003251234 2307 68-69 0.1350105039741294 2307 70-71 0.29138580770651146 2307 72-73 -0.06835920888715918 2307 74-75 0.026897308435650302 2307 76-77 0.14687926050945066 2307 78-79 0.18187280210221957 2307 80-81 0.47290814570432005 2307 82-83 0.5004119957011639 2307 84-85 0.41952556706862154 2307 86-87 0.4777105105599482 2307 88-89 0.30613265174352833 2307 90-91 0.29932944654257554 2307 92-93 0.3782349432136911 2307 94-95 0.31871151376421736 2307 96-97 0.3737008612784045 2307 98-99 0.4124445303141684 2307 100-101 0.26598360232223683 2201 1 -0.25685615356728775 2201 2 -0.2789756544198525 2201 3 -0.34859020965457077 2201 4 -0.2702121455927653 2201 5 -0.35617920242873424 2201 6 -0.4683540446890504 2201 7 -0.002232282370371763 2201 8 -0.4937918527165408 2201 9 -0.21076014367613993 2201 10-11 -0.31625005355408575 2201 12-13 -0.3031746516483551 2201 14-15 0.02923788874966249 2201 16-17 -0.11704564864018607 2201 18-19 -0.1540471612903147 2201 20-21 -0.21551310958952996 2201 22-23 -0.16051410509330566 2201 24-25 -0.05572913902607013 2201 26-27 -0.47865996674912736 2201 28-29 -0.27642007770877797 2201 30-31 -0.25256562808692706 2201 32-33 -0.1959673993866815 2201 34-35 -0.43966245083802846 2201 36-37 -0.1529479539959553 2201 38-39 -0.35480053477417783 2201 40-41 -0.3156789380944858 2201 42-43 -0.2774028008738654 2201 44-45 -0.16446090196652818 2201 46-47 -0.34147345246263683 2201 48-49 -0.2655070171347731 2201 50-51 -0.27293486341120143 2201 52-53 -0.32736777643579273 2201 54-55 -0.1551346926510746 2201 56-57 -0.16701994779499074 2201 58-59 0.05560950114111307 2201 60-61 -0.23957446344607547 2201 62-63 -0.040128079668754424 2201 64-65 -0.14883785949884043 2201 66-67 -0.17986493294248618 2201 68-69 -0.2757139558780253 2201 70-71 -0.23293848282757068 2201 72-73 -0.4368522576865388 2201 74-75 -0.19435647832105474 2201 76-77 -0.09948404334631533 2201 78-79 0.07359366279728619 2201 80-81 0.14257334448847203 2201 82-83 0.05636496987914086 2201 84-85 0.1317291552031925 2201 86-87 -0.019362208181462393 2201 88-89 -0.0018074368091021142 2201 90-91 -0.15822896193904867 2201 92-93 -0.001163250471787336 2201 94-95 -0.07846644830047111 2201 96-97 -0.10230740535213201 2201 98-99 -0.1260309334925651 2201 100-101 -0.15478598477489314 1101 1 -0.011796377419429405 1101 2 -0.14420455332750493 1101 3 -0.13419234499763988 1101 4 -0.15039068898374808 1101 5 -0.15452161994213753 1101 6 -0.0890502741935677 1101 7 0.20692815995069225 1101 8 -0.11129179664135336 1101 9 0.029151930432824713 1101 10-11 -0.01170011299378615 1101 12-13 -0.029208117319136306 1101 14-15 0.20974391122459224 1101 16-17 0.0599837123270035 1101 18-19 0.10951742610847504 1101 20-21 0.03584390990221209 1101 22-23 0.1408788025172285 1101 24-25 0.16407437352355458 1101 26-27 -0.0724569297170774 1101 28-29 -0.014616251138107828 1101 30-31 0.10406694486689361 1101 32-33 0.016950080370172316 1101 34-35 -0.14457800160850098 1101 36-37 0.12634011544755452 1101 38-39 -0.09488083443996942 1101 40-41 -0.07490330612714047 1101 42-43 0.020513445228914406 1101 44-45 0.1406497904498636 1101 46-47 0.07217076169970937 1101 48-49 0.0013099310293256394 1101 50-51 -0.0991354331350891 1101 52-53 -0.0785397478150145 1101 54-55 0.031848013761688776 1101 56-57 0.0770192599747972 1101 58-59 0.1961395237954875 1101 60-61 0.13043204127545494 1101 62-63 0.15683600978238843 1101 64-65 0.14666715362275085 1101 66-67 0.17556763103930706 1101 68-69 0.14636957371845938 1101 70-71 0.19055814144625316 1101 72-73 -0.1961607384976105 1101 74-75 0.0452079296371366 1101 76-77 0.18970690387572375 1101 78-79 0.2699241026510393 1101 80-81 0.3326850462582023 1101 82-83 0.15591524689055802 1101 84-85 0.2653507149905572 1101 86-87 0.3596836164602202 1101 88-89 0.2213830169512967 1101 90-91 0.15061073032033434 1101 92-93 0.254775111684193 1101 94-95 0.22913958987551553 1101 96-97 0.14063272877971045 1101 98-99 0.10305862979389246 1101 100-101 0.04952956150956567 2304 1 0.10255524557398488 2304 2 -0.09089309130888878 2304 3 -0.009156239109916697 2304 4 -0.019073297284727175 2304 5 -0.08165074984929532 2304 6 0.07061719044808257 2304 7 0.24953263082082344 2304 8 0.03302371463866649 2304 9 0.059944175433635394 2304 10-11 -0.02140855854621293 2304 12-13 0.012565587129593325 2304 14-15 0.15700156379269004 2304 16-17 0.0935354692895416 2304 18-19 0.15978264748748217 2304 20-21 0.14119728896313433 2304 22-23 0.1116498484498436 2304 24-25 0.1514234636234093 2304 26-27 0.10740411968022556 2304 28-29 0.08159377195164552 2304 30-31 0.15604912235459523 2304 32-33 0.06824141333652278 2304 34-35 0.033203761748502814 2304 36-37 0.18905441388751143 2304 38-39 0.10659849276130728 2304 40-41 0.10074470121446666 2304 42-43 0.10318472695271197 2304 44-45 0.09496182115938012 2304 46-47 0.0626259908755813 2304 48-49 0.07468678667600415 2304 50-51 0.2304670202540251 2304 52-53 0.16455013871267 2304 54-55 0.28892010722316286 2304 56-57 0.38835655638498423 2304 58-59 0.21834953036153593 2304 60-61 0.15575610452692956 2304 62-63 0.23791982405973755 2304 64-65 0.19026707189394898 2304 66-67 0.3368590000188547 2304 68-69 0.1850870809351406 2304 70-71 0.21884617104826276 2304 72-73 0.24816228789127592 2304 74-75 0.5520750655841269 2304 76-77 0.41460355391664194 2304 78-79 0.4854850173483527 2304 80-81 0.45230526898717827 2304 82-83 0.16950761086205546 2304 84-85 0.20520969256926236 2304 86-87 0.2736359906891259 2304 88-89 0.24714967192073445 2304 90-91 0.2009650843858708 2304 92-93 0.3195372375692038 2304 94-95 0.28494035888458313 2304 96-97 0.16613680510398154 2304 98-99 0.2241975241524301 2304 100-101 0.2274060978567931 2202 1 -0.02313584827747306 2202 2 -0.2587118604812133 2202 3 -0.15431618557129312 2202 4 -0.23071647587475752 2202 5 -0.23201277913150165 2202 6 0.04818044021709511 2202 7 0.056556614986376985 2202 8 0.03215424033194125 2202 9 -0.10688826964998555 2202 10-11 -0.1089824044001233 2202 12-13 -0.09666307549473885 2202 14-15 -0.057888924273221676 2202 16-17 -0.14371626532906845 2202 18-19 -0.06475045634039844 2202 20-21 -0.023318881777839806 2202 22-23 0.14129329209880126 2202 24-25 0.010640921308898044 2202 26-27 -0.06661084799527828 2202 28-29 -0.17610650565583796 2202 30-31 -0.042088281506671876 2202 32-33 -0.09803932419884376 2202 34-35 -0.11411069302785393 2202 36-37 -0.059097157165531655 2202 38-39 -0.1433959228612096 2202 40-41 -0.12347478493885689 2202 42-43 -0.1409157702281547 2202 44-45 -0.043860906190751336 2202 46-47 -0.05749353458308093 2202 48-49 -0.017179093510897303 2202 50-51 -0.049596626693286794 2202 52-53 -0.13623656619439828 2202 54-55 0.1205456261541542 2202 56-57 0.35459676315988986 2202 58-59 0.02774440239293341 2202 60-61 -0.06323363020524653 2202 62-63 0.012619935752113065 2202 64-65 3.586805221544864E-5 2202 66-67 0.17185953336912263 2202 68-69 0.07003045100539396 2202 70-71 0.16230496415597884 2202 72-73 0.21136809108951127 2202 74-75 0.41088046134233025 2202 76-77 0.2497306343264185 2202 78-79 0.2647864631062973 2202 80-81 0.14196114927394987 2202 82-83 0.11195224242268154 2202 84-85 0.10267497378283608 2202 86-87 0.14928066063360035 2202 88-89 0.10475738153619218 2202 90-91 0.07967353921057096 2202 92-93 -0.0031555449111628775 2202 94-95 -0.00941490411016943 2202 96-97 0.016016484082602034 2202 98-99 0.05823013894323026 2202 100-101 -0.0239978484141119 1102 1 -0.010533513189564303 1102 2 -0.17044568224111956 1102 3 -0.07265285190646864 1102 4 -0.15854065486482938 1102 5 -0.046653813546924994 1102 6 0.04377454734137132 1102 7 -0.0385504970360131 1102 8 0.16787877813870722 1102 9 -0.024917438607914733 1102 10-11 -0.051763041316867486 1102 12-13 -0.0755940771902246 1102 14-15 -0.10550925076977791 1102 16-17 -0.08928351820556912 1102 18-19 -0.09860941414272162 1102 20-21 -0.07260402340255467 1102 22-23 0.01139638110679897 1102 24-25 -0.07365695815378359 1102 26-27 0.12913560395890755 1102 28-29 -0.10101464773558178 1102 30-31 -0.03125299908408863 1102 32-33 -0.19702679763724973 1102 34-35 -0.10274695807306955 1102 36-37 -0.07928985552192813 1102 38-39 -0.1053153177405548 1102 40-41 -0.1267205106577336 1102 42-43 -0.06746067206072581 1102 44-45 -0.014626681911416028 1102 46-47 -0.0036973850710779743 1102 48-49 -0.05264237408196237 1102 50-51 0.013387124165213038 1102 52-53 0.01000906434983051 1102 54-55 0.15302806124388724 1102 56-57 0.2659819309390983 1102 58-59 0.08408936204529738 1102 60-61 0.15170130334100662 1102 62-63 0.19967233861544287 1102 64-65 0.07341571590444573 1102 66-67 0.17557555175897477 1102 68-69 0.16126088565095387 1102 70-71 0.1504597972164632 1102 72-73 0.2992973868350717 1102 74-75 0.2965525615380855 1102 76-77 0.2503788508307352 1102 78-79 0.2137662480203204 1102 80-81 0.21415406073785448 1102 82-83 0.05736768672587189 1102 84-85 0.12473312570920925 1102 86-87 0.16301626285258308 1102 88-89 0.09075785823778304 1102 90-91 0.019349664644213505 1102 92-93 0.10696012476367045 1102 94-95 0.1126402569867615 1102 96-97 0.12422798507745014 1102 98-99 -0.024871474138397076 1102 100-101 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40-41 0.08973985437187082 1213 42-43 0.17581167174815704 1213 44-45 0.07504005249216306 1213 46-47 0.11873239378434874 1213 48-49 0.14048772221624262 1213 50-51 0.19709085673200377 1213 52-53 0.15731612759966396 1213 54-55 -0.027588791830808645 1213 56-57 -0.012449568302315583 1213 58-59 -0.18512983525259585 1213 60-61 0.04531210152512699 1213 62-63 0.09780506876145267 1213 64-65 0.13802094453624392 1213 66-67 0.22096281616770597 1213 68-69 0.2368533041151082 1213 70-71 0.19569366711819924 1213 72-73 0.5209206361154486 1213 74-75 0.3403740675466125 1213 76-77 0.1455023851980073 1213 78-79 0.1529867573098116 1213 80-81 0.13882231428960168 1213 82-83 0.107391805669252 1213 84-85 0.1989570777435823 1213 86-87 0.21921364755022665 1213 88-89 0.10047695393030764 1213 90-91 0.21557255919362817 1213 92-93 0.17923137952087131 1213 94-95 0.2740097986577865 1213 96-97 0.27965139337515055 1213 98-99 0.16948849098038465 1213 100-101 0.2997388119243354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2810.0 3 881.0 4 851.0 5 680.0 6 1796.0 7 9277.0 8 49821.0 9 144327.0 10 167827.0 11 95055.0 12 48053.0 13 28874.0 14 20088.0 15 15954.0 16 14201.0 17 13574.0 18 13957.0 19 14717.0 20 15864.0 21 17446.0 22 19323.0 23 21370.0 24 24215.0 25 28216.0 26 34115.0 27 41452.0 28 51564.0 29 64940.0 30 79078.0 31 100593.0 32 139751.0 33 202845.0 34 310798.0 35 530652.0 36 1067150.0 37 1740744.0 38 376900.0 39 78.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.427698786037105 23.945562355517993 22.463710997935497 14.163027860509406 2 31.854237755156454 25.602466704242822 29.751162000495395 12.792133540105329 3 34.897791980740124 20.7358256887995 28.239747039450762 16.126635291009613 4 27.565605278621184 18.187029392984034 33.88519690224836 20.36216842614642 5 33.248570780370386 16.72949720469147 31.40109643700727 18.62083557793088 6 30.91425418833297 17.007163062223647 35.70933445638046 16.36924829306293 7 36.07307222623546 18.772833480148666 27.72802941590281 17.426064877713067 8 27.790038366827734 31.038885426113183 23.93310608072503 17.237970126334055 9 31.60263422224492 27.361904967871858 23.194760487829434 17.840700322053788 10-11 26.737237141471482 29.832602043903535 26.588835084739266 16.841325729885725 12-13 29.350671231203634 24.146866448763095 26.28099528037152 20.221467039661746 14-15 26.40668737326087 24.305614499293068 27.968778711406188 21.318919416039876 16-17 27.649373731726897 25.31662812076627 28.976024543853317 18.05797360365352 18-19 27.35097080092212 25.47552399771615 29.256937420474937 17.9165677808868 20-21 25.808465594331032 27.822945314735424 28.423890875351866 17.944698215581678 22-23 28.84861576434332 25.728851360667665 27.40762332364346 18.014909551345553 24-25 25.528507846484306 28.818882158903904 27.330490531212902 18.32211946339889 26-27 22.687943506876966 31.68296759879944 26.99982419567703 18.629264698646562 28-29 24.109260688303458 29.933705381883495 26.89066866266104 19.06636526715201 30-31 26.909947912642068 26.94416070309241 27.20442001219792 18.9414713720676 32-33 23.780658291703887 28.66445288348249 28.948357694022487 18.60653113079114 34-35 27.525100172145123 26.833451186486545 27.847027760472614 17.794420880895714 36-37 24.857728510691143 27.13612085242168 29.2494212723759 18.756729364511273 38-39 21.11293498834714 29.35678523001795 30.831740222903132 18.69853955873178 40-41 21.696056832672962 30.709101777705538 28.466625965236243 19.128215424385257 42-43 24.80863536528273 27.46663611765534 29.523394444408524 18.201334072653406 44-45 22.862337185112562 28.929424370885215 29.900379745487033 18.307858698515187 46-47 24.22093960692306 29.94282323558362 27.483057648752517 18.353179508740794 48-49 21.542207376814883 29.751285397431694 29.147950170935573 19.558557054817854 50-51 20.062756074645417 27.28415783400476 31.399620196069023 21.25346589528081 52-53 25.00865231725036 26.67905716739204 27.944230363519335 20.368060151838264 54-55 27.398400964356064 26.68196234440859 27.144046589906033 18.77559010132931 56-57 28.11393078395476 26.177667615622457 26.66324488977927 19.04515671064351 58-59 26.494435191589634 26.0032756714521 27.34526485749354 20.15702427946472 60-61 22.47615585212819 26.581187797701972 26.614748604796144 24.327907745373693 62-63 24.52715117919941 26.729427093358932 26.718487386162654 22.024934341279 64-65 22.694605589617176 27.77281300762095 29.330225704782446 20.202355697979428 66-67 21.016609692704755 27.817607457747474 28.447765270311482 22.718017579236292 68-69 20.266537023066412 26.6341956902302 33.19912332186664 19.900143964836754 70-71 19.71889691976986 30.402852530091533 31.014557513828546 18.86369303631006 72-73 19.558616268764144 35.14487178005455 26.702351596143597 18.59416035503772 74-75 20.417604232714872 35.07151587595664 25.80789317170435 18.702986719624136 76-77 20.834098098580508 34.14675539490506 26.215640049135462 18.80350645737897 78-79 20.493253863957534 33.900343786470884 26.96252967724697 18.64387267232461 80-81 19.719108870114475 34.28728494813271 26.701995735937583 19.291610445815234 82-83 19.016437408639465 33.92184303917498 27.09754958720082 19.96416996498474 84-85 19.19427112168414 32.9742300970925 27.505364747288407 20.32613403393495 86-87 19.173593637036763 32.743072984114626 28.116881780980112 19.966451597868502 88-89 19.628276166316212 33.0647752868213 27.671589811077467 19.63535873578502 90-91 20.231197508642985 31.990881797904674 27.418963854077088 20.358956839375253 92-93 19.935095967666115 32.55659762858282 26.84685573482925 20.661450668921812 94-95 19.59456132749317 32.90073606333572 26.788568198048623 20.716134411122482 96-97 20.28016126922554 33.124545277710446 26.536429985488024 20.058863467575986 98-99 20.771994498758144 31.904963388776636 26.584674810262136 20.73836730220308 100-101 20.760121271000486 31.83815288215258 26.140429517239173 21.261296329607763 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1525.0 1 1209.0 2 630.0 3 378.5 4 394.5 5 489.5 6 663.5 7 905.0 8 1192.5 9 1452.0 10 1710.5 11 1860.5 12 1804.0 13 1625.0 14 1430.0 15 1250.5 16 1094.5 17 1010.0 18 997.0 19 1049.0 20 1127.0 21 1256.0 22 1497.0 23 1775.5 24 2090.0 25 2554.5 26 3202.0 27 3929.5 28 4902.5 29 6155.0 30 8014.0 31 11638.0 32 21349.5 33 40516.5 34 71679.0 35 114963.5 36 173698.5 37 253747.0 38 330041.5 39 357815.5 40 361025.5 41 342336.0 42 320778.5 43 346561.5 44 355451.5 45 340812.0 46 352136.0 47 331600.5 48 264630.0 49 214183.5 50 180265.5 51 151058.0 52 121692.0 53 91897.5 54 70618.5 55 54821.0 56 42872.0 57 32452.0 58 23545.0 59 17680.0 60 13602.5 61 10673.5 62 8228.0 63 6042.0 64 4644.0 65 3689.5 66 2884.5 67 2228.5 68 1728.0 69 1339.5 70 1019.0 71 798.0 72 595.5 73 440.5 74 351.5 75 286.0 76 222.0 77 171.5 78 138.0 79 99.0 80 87.0 81 68.5 82 43.0 83 35.5 84 31.5 85 28.5 86 24.5 87 16.5 88 9.5 89 8.5 90 7.5 91 4.0 92 4.5 93 4.0 94 2.0 95 2.5 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29957329046213166 2 0.2761969183480382 3 0.24762983006575331 4 0.23497972807544035 5 0.24149534732152694 6 0.19080419257411788 7 0.15599372540421794 8 0.15372505575028805 9 0.13321628207876204 10-11 0.11876939372253661 12-13 0.139913394897163 14-15 0.1431258311271277 16-17 0.13652853977349966 18-19 0.12441384382151413 20-21 0.13023071281419032 22-23 0.1584892620235408 24-25 0.566350692407053 26-27 0.22228425269204877 28-29 0.1964395679944797 30-31 0.29542616233474783 32-33 0.3272601349186918 34-35 0.3364890830708785 36-37 0.3275595993130105 38-39 0.3354545697086865 40-41 0.3381134505430923 42-43 0.34858562966563256 44-45 0.3290932199990671 46-47 0.34905751295364995 48-49 0.3453005960067421 50-51 0.3515984229660514 52-53 0.33637111224887417 54-55 0.3283127976381152 56-57 0.3364527843564156 58-59 0.32920211614245576 60-61 0.30531756202588206 62-63 0.29199593381800587 64-65 0.3028764734782535 66-67 0.3187390116985312 68-69 0.30543553284788644 70-71 0.28616091546809824 72-73 0.2821771315557974 74-75 0.27429123583873716 76-77 0.2764419346706627 78-79 0.2854712398933036 80-81 0.29491798033226757 82-83 0.31793136530173216 84-85 0.3091561510803314 86-87 0.31173335980719574 88-89 0.31725076440555317 90-91 0.3101271416922134 92-93 0.2909977191702767 94-95 0.2869867112221287 96-97 0.30466418516555027 98-99 0.3132397564574052 100-101 0.3148006011793089 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5509837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.92860415065641 #Duplication Level Percentage of deduplicated Percentage of total 1 87.39154024064841 28.776814347004713 2 4.824677481906357 3.177397899125603 3 1.9690818012607747 1.9451734552193263 4 1.2200316815845809 1.606957611766334 5 0.695092584915177 1.1444214288364194 6 0.5902009999026996 1.1660697057070557 7 0.34054244816739393 0.784951123053968 8 0.3453275500849026 0.9096923359249385 9 0.21645640953708123 0.6414846682967024 >10 1.821123158620326 12.50634296030316 >50 0.2622192041012532 6.014462497087006 >100 0.25723647535127464 18.24137910403995 >500 0.044107258172561246 9.94437449286857 >1k 0.02181182293885265 11.69871080379754 >5k 4.957968267226717E-4 1.1317168716830952 >10k+ 5.508598197601766E-5 0.3100506952856482 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT 17093 0.31022696315698634 Illumina Single End PCR Primer 1 (97% over 34bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9589 0.1740341864922683 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 9214 0.16722817753047867 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 8498 0.15423323775276837 No Hit GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8328 0.15114784702342376 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 5688 0.10323354393242486 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5513 0.10005740641692304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005535553955588886 0.0 0.0 2 0.0061163333869949325 0.0 0.0 3 0.006551917960549468 0.0 0.0 4 0.007005651891335442 0.0 0.0 5 0.007949418467370269 0.0 0.0 6 0.008766139542785023 0.0 0.0 7 0.009963997120059994 0.0 0.0 8 0.011089257268409211 0.0 0.0 9 0.012232666773989865 0.0 0.0 10-11 0.017849892837120226 0.0 0.0 12-13 0.023095057077006088 0.0 0.0 14-15 0.028276698566581916 0.0 0.0 16-17 0.03275051512413162 0.0 9.074678615719486E-6 18-19 0.03834959183003055 0.0 1.814935723143897E-5 20-21 0.044447775859794036 0.0 2.7224035847158455E-5 22-23 0.051163038035426454 0.0 3.629871446287794E-5 24-25 0.05847722899969636 0.0 3.629871446287794E-5 26-27 0.06643572214568234 0.0 3.629871446287794E-5 28-29 0.07490239729414863 0.0 3.629871446287794E-5 30-31 0.11688186057046696 0.0 3.629871446287794E-5 32-33 0.12780777362379322 0.0 3.629871446287794E-5 34-35 0.13777177074385322 0.0 3.629871446287794E-5 36-37 0.14877935590472097 0.0 3.629871446287794E-5 38-39 0.16108462010763658 0.0 3.629871446287794E-5 40-41 0.1749779530683031 0.0 3.629871446287794E-5 42-43 0.18872609117111813 0.0 3.629871446287794E-5 44-45 0.2035722653864352 0.0 3.629871446287794E-5 46-47 0.2195346250714858 0.0 3.629871446287794E-5 48-49 0.2366857676551956 0.0 3.629871446287794E-5 50-51 0.25587871292744235 0.0 3.629871446287794E-5 52-53 0.27689566860144865 0.0 3.629871446287794E-5 54-55 0.29799429638299646 0.0 3.629871446287794E-5 56-57 0.32076266502983664 0.0 3.629871446287794E-5 58-59 0.3456091350796766 0.0 3.629871446287794E-5 60-61 0.37176235885018016 0.0 3.629871446287794E-5 62-63 0.39967607027213325 0.0 5.444807169431691E-5 64-65 0.42854262294873696 0.0 5.444807169431691E-5 66-67 0.46076680671315684 0.0 5.444807169431691E-5 68-69 0.4956770953478297 0.0 5.444807169431691E-5 70-71 0.5325747385993451 0.0 5.444807169431691E-5 72-73 0.5715686326110918 0.0 5.444807169431691E-5 74-75 0.6139836804609646 0.0 5.444807169431691E-5 76-77 0.6616529672293391 0.0 5.444807169431691E-5 78-79 0.7133332619458616 0.0 5.444807169431691E-5 80-81 0.7691334607539206 0.0 5.444807169431691E-5 82-83 0.828745024580582 0.0 5.444807169431691E-5 84-85 0.893383960360352 0.0 5.444807169431691E-5 86-87 0.9647926063874485 0.0 5.444807169431691E-5 88-89 1.041646059583977 0.0 5.444807169431691E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4890 0.0 89.30407 9 CGGGAGA 5995 0.0 89.22481 4 CGGAAAG 3410 0.0 87.40901 5 ATTCGGA 1270 0.0 86.726875 2 CCGGAGA 1460 0.0 86.47284 4 AGAGCGG 5285 0.0 86.402336 8 GTTCGGA 1260 0.0 85.90803 2 GAGAGCG 5705 0.0 85.778046 7 CGGAGAG 3060 0.0 85.463585 5 GTCCGGA 600 0.0 85.45588 2 AAGCGGC 1800 0.0 83.24058 9 TCGGAGA 1835 0.0 83.02711 4 TTCGGAG 1240 0.0 81.91488 3 TCGGGAG 6720 0.0 79.46098 3 GGATCGG 2720 0.0 79.253 1 ACCGGAA 950 0.0 77.94203 3 AAGGGGC 5625 0.0 77.8036 9 TCGGGAA 2695 0.0 75.73216 3 AAGCGTC 500 0.0 74.91651 9 AAAGCGG 2260 0.0 73.644936 8 >>END_MODULE