FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34042-1.34200000035847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34042-1.34200000035847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5509837
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT170930.31022696315698634Illumina Single End PCR Primer 1 (97% over 34bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC95890.1740341864922683No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG92140.16722817753047867No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG84980.15423323775276837No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC83280.15114784702342376No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA56880.10323354393242486No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA55130.10005740641692304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC48900.089.304079
CGGGAGA59950.089.224814
CGGAAAG34100.087.409015
ATTCGGA12700.086.7268752
CCGGAGA14600.086.472844
AGAGCGG52850.086.4023368
GTTCGGA12600.085.908032
GAGAGCG57050.085.7780467
CGGAGAG30600.085.4635855
GTCCGGA6000.085.455882
AAGCGGC18000.083.240589
TCGGAGA18350.083.027114
TTCGGAG12400.081.914883
TCGGGAG67200.079.460983
GGATCGG27200.079.2531
ACCGGAA9500.077.942033
AAGGGGC56250.077.80369
TCGGGAA26950.075.732163
AAGCGTC5000.074.916519
AAAGCGG22600.073.6449368
Produced by FastQC (version 0.11.2)