##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_34012-1.34200000035798.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2911263 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.583296665399175 33.0 33.0 33.0 22.0 33.0 2 29.925246877386208 33.0 33.0 33.0 15.0 33.0 3 29.73462926571732 33.0 33.0 33.0 15.0 33.0 4 33.01383557583083 37.0 37.0 37.0 15.0 37.0 5 33.11501606003992 37.0 37.0 37.0 15.0 37.0 6 33.78897028540534 37.0 37.0 37.0 22.0 37.0 7 33.21656786075322 37.0 37.0 37.0 15.0 37.0 8 33.437665370665584 37.0 37.0 37.0 22.0 37.0 9 32.78824379659275 37.0 37.0 37.0 15.0 37.0 10-11 32.87412507904645 37.0 37.0 37.0 15.0 37.0 12-13 32.83481516441489 37.0 37.0 37.0 15.0 37.0 14-15 34.39397900498856 40.0 37.0 40.0 15.0 40.0 16-17 34.31850385897805 40.0 37.0 40.0 15.0 40.0 18-19 34.382488287729416 40.0 37.0 40.0 15.0 40.0 20-21 34.332536256600655 40.0 37.0 40.0 15.0 40.0 22-23 35.2429799368865 40.0 37.0 40.0 22.0 40.0 24-25 34.47989137360658 40.0 37.0 40.0 15.0 40.0 26-27 34.27304506669442 37.0 37.0 40.0 15.0 40.0 28-29 33.979834181934095 37.0 37.0 40.0 15.0 40.0 30-31 34.007027534097745 37.0 37.0 40.0 10.5 40.0 32-33 33.905370280871225 37.0 37.0 40.0 10.5 40.0 34-35 33.935181740708416 37.0 37.0 40.0 15.0 40.0 36-37 33.79588429489194 37.0 33.0 40.0 10.5 40.0 38-39 33.617840950817566 37.0 33.0 40.0 6.0 40.0 40-41 33.65941654876251 37.0 33.0 40.0 10.5 40.0 42-43 33.518080468854926 37.0 33.0 40.0 10.5 40.0 44-45 33.32793825222936 37.0 33.0 40.0 6.0 40.0 46-47 33.22524519426791 37.0 33.0 40.0 6.0 40.0 48-49 33.136948980562735 37.0 33.0 40.0 6.0 40.0 50-51 32.26687695340476 37.0 33.0 38.5 15.0 38.5 52-53 32.63337029323699 37.0 33.0 38.5 10.5 40.0 54-55 33.24733388910586 37.0 33.0 40.0 10.5 40.0 56-57 33.625267967888846 37.0 33.0 40.0 15.0 40.0 58-59 33.035603619460005 37.0 33.0 40.0 6.0 40.0 60-61 32.81619781517506 37.0 33.0 40.0 6.0 40.0 62-63 32.444827554226464 37.0 33.0 40.0 6.0 40.0 64-65 32.28738798246671 37.0 33.0 40.0 6.0 40.0 66-67 32.163426320466414 37.0 33.0 37.0 6.0 40.0 68-69 32.30054687604658 37.0 33.0 37.0 15.0 40.0 70-71 31.908327416657308 37.0 33.0 37.0 15.0 40.0 72-73 32.24011313989839 37.0 33.0 37.0 15.0 40.0 74-75 32.454508404084415 37.0 33.0 37.0 22.0 37.0 76-77 32.18668993491828 37.0 33.0 37.0 22.0 37.0 78-79 31.703024941408593 37.0 33.0 37.0 18.5 37.0 80-81 31.089924716523377 37.0 33.0 37.0 15.0 37.0 82-83 30.717084646766715 35.0 27.0 37.0 15.0 37.0 84-85 30.401669309849368 33.0 27.0 37.0 15.0 37.0 86-87 30.214720724304193 33.0 27.0 37.0 6.0 37.0 88-89 30.078576377331764 33.0 27.0 37.0 6.0 37.0 90-91 29.932125507039384 33.0 27.0 37.0 6.0 37.0 92-93 29.754715393284634 33.0 27.0 37.0 6.0 37.0 94-95 29.620846690937917 33.0 27.0 37.0 6.0 37.0 96-97 29.487181336760024 33.0 27.0 37.0 6.0 37.0 98-99 29.343579573539046 33.0 27.0 37.0 6.0 37.0 100-101 28.174232111629898 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.3551236580184174 2306 2 -0.21702417546395125 2306 3 -0.2868654643830517 2306 4 -0.08575710435266615 2306 5 -0.2173187775763452 2306 6 -0.46909610928068446 2306 7 -0.010175160712734055 2306 8 -0.36851010398653017 2306 9 0.008312462269607579 2306 10-11 -0.11234549860482446 2306 12-13 -0.13178244879586032 2306 14-15 0.13075526789479142 2306 16-17 0.1255552828427824 2306 18-19 0.09254626232334573 2306 20-21 0.14076078250167257 2306 22-23 -0.14697154452215955 2306 24-25 0.06424596035188301 2306 26-27 -0.35154187455853503 2306 28-29 -0.13936625390702773 2306 30-31 -0.1300900231982567 2306 32-33 0.02185047161475495 2306 34-35 -0.08316138108924775 2306 36-37 -0.27887926652788053 2306 38-39 -0.06324487721543903 2306 40-41 -0.057767020487673904 2306 42-43 -0.025341765689077533 2306 44-45 0.07081693596978056 2306 46-47 0.004042118471652145 2306 48-49 -0.10070320511896824 2306 50-51 0.009229934597662748 2306 52-53 -0.10068544545210756 2306 54-55 -0.015435167473441425 2306 56-57 -0.12857112699371953 2306 58-59 0.0055466351995292484 2306 60-61 -0.2540994731051711 2306 62-63 0.018535050080586757 2306 64-65 -0.13888692974310146 2306 66-67 -0.05057865706257303 2306 68-69 -0.4083050755138089 2306 70-71 -0.19717328543532986 2306 72-73 -0.35191213400613464 2306 74-75 -0.4151159692938009 2306 76-77 -0.13363345198243337 2306 78-79 -0.025152732595163485 2306 80-81 0.03441206669360142 2306 82-83 0.1395644796408817 2306 84-85 0.17590210536689455 2306 86-87 0.1408266753813905 2306 88-89 0.14928134081185718 2306 90-91 -0.10488322491295676 2306 92-93 -0.20309573317985397 2306 94-95 -0.14110816419931282 2306 96-97 -0.15907383193911429 2306 98-99 0.07309626600430263 2306 100-101 0.017141551507439345 2307 1 -0.0707539965812991 2307 2 0.08867831920452929 2307 3 -0.08011613741987134 2307 4 0.10315374939722943 2307 5 -0.004949452273336874 2307 6 -0.28757159762308504 2307 7 0.13155025256313024 2307 8 -0.3237358108673405 2307 9 0.2180939648505671 2307 10-11 0.11249450463353838 2307 12-13 0.022082324861884217 2307 14-15 0.34286152726529906 2307 16-17 0.3285433920986591 2307 18-19 0.284689434957933 2307 20-21 0.14973043922471163 2307 22-23 0.034267355622439766 2307 24-25 0.28543388698856376 2307 26-27 -0.09861141025280062 2307 28-29 0.12227889710224815 2307 30-31 0.11366818159964254 2307 32-33 0.15380975429637544 2307 34-35 0.1279614810516989 2307 36-37 0.039155865635336795 2307 38-39 0.10618013874579901 2307 40-41 0.05048773637361137 2307 42-43 0.14797738442452157 2307 44-45 0.21129363152553537 2307 46-47 0.17643187826899265 2307 48-49 0.09264841063055229 2307 50-51 -0.06800241093180404 2307 52-53 0.01974126040287416 2307 54-55 0.08220769208501366 2307 56-57 -0.11849878642427569 2307 58-59 0.2314538642349575 2307 60-61 0.11370615529651928 2307 62-63 0.2592402758387067 2307 64-65 0.2697667814131677 2307 66-67 0.24731599563813234 2307 68-69 0.1652552098312583 2307 70-71 0.11559202224522735 2307 72-73 -0.09093269250115554 2307 74-75 -0.025737192268564968 2307 76-77 0.14820239364593135 2307 78-79 0.28142862979327177 2307 80-81 0.28374979026633795 2307 82-83 0.34125396345981684 2307 84-85 0.4128933566568591 2307 86-87 0.3695003496243956 2307 88-89 0.24311041046847492 2307 90-91 0.2643948348553806 2307 92-93 0.32489419509445483 2307 94-95 0.36480410684775677 2307 96-97 0.4092508572136957 2307 98-99 0.24336940571209098 2307 100-101 0.26981325403695067 2201 1 -0.06153726215015354 2201 2 -0.16326819993929576 2201 3 -0.17602568785969552 2201 4 -0.01793013795974474 2201 5 -0.07626006609422831 2201 6 -0.1240247750933321 2201 7 0.25372774480871385 2201 8 -0.273934800282305 2201 9 -0.002980836231749606 2201 10-11 -0.047338135751793686 2201 12-13 -0.06608629344093231 2201 14-15 0.23557462192611212 2201 16-17 0.17088887521588703 2201 18-19 0.15681303124805623 2201 20-21 0.12115477494630511 2201 22-23 0.0839847724806404 2201 24-25 0.17051575432321897 2201 26-27 -0.14359028705534627 2201 28-29 0.040980363244393914 2201 30-31 0.06589063655803074 2201 32-33 0.1801604290239993 2201 34-35 0.07505956403903724 2201 36-37 -0.03500343875773382 2201 38-39 -0.05255880110593125 2201 40-41 -0.04137191798449891 2201 42-43 -0.02551068338394913 2201 44-45 0.06008424836881687 2201 46-47 -0.04133054529869895 2201 48-49 0.01833167457343876 2201 50-51 0.026184668768209463 2201 52-53 -0.02545626086139663 2201 54-55 0.1648535770068662 2201 56-57 0.10594577917049008 2201 58-59 0.1452251177487085 2201 60-61 0.0751595001455101 2201 62-63 -0.02553141477268639 2201 64-65 0.01973775315676818 2201 66-67 0.08468583475368519 2201 68-69 -0.019796433684540204 2201 70-71 0.09422961665879726 2201 72-73 -0.23247341861883797 2201 74-75 -0.10631532801367882 2201 76-77 0.02421577629382199 2201 78-79 0.20149456745377847 2201 80-81 0.40906338629821803 2201 82-83 0.27309504355962844 2201 84-85 0.2697568894195719 2201 86-87 0.2294359040258378 2201 88-89 0.09225864395321537 2201 90-91 0.2754115124733971 2201 92-93 0.2285727016552208 2201 94-95 0.17318930368037755 2201 96-97 0.03764144842411099 2201 98-99 0.11977493284513585 2201 100-101 0.08839583344860102 1101 1 0.20812301615104545 1101 2 0.20372237145961947 1101 3 0.1354195324782168 1101 4 0.32793625001865934 1101 5 0.2142926832253238 1101 6 0.09072265486953057 1101 7 0.5424348881308347 1101 8 0.09850022083923449 1101 9 0.3280008889260202 1101 10-11 0.3383058118393265 1101 12-13 0.26748376657205597 1101 14-15 0.49902608108085644 1101 16-17 0.4308937663356929 1101 18-19 0.4762766970895669 1101 20-21 0.49198469945064005 1101 22-23 0.43521054340380516 1101 24-25 0.48500165012828944 1101 26-27 0.2371095829253136 1101 28-29 0.44322236107330326 1101 30-31 0.5007425042444353 1101 32-33 0.4537075641121504 1101 34-35 0.4287555264020497 1101 36-37 0.3711579646769607 1101 38-39 0.4060075201913804 1101 40-41 0.48827600935950244 1101 42-43 0.4675592332382834 1101 44-45 0.5425085899589916 1101 46-47 0.4740041709195779 1101 48-49 0.5150556799431172 1101 50-51 0.35792627689099277 1101 52-53 0.2996664129976736 1101 54-55 0.5263759468423785 1101 56-57 0.6046307189199922 1101 58-59 0.8274242567005032 1101 60-61 0.6622962069439922 1101 62-63 0.5736325204322981 1101 64-65 0.7086418608176999 1101 66-67 0.6677954609348973 1101 68-69 0.6434168912740716 1101 70-71 0.5888430510518141 1101 72-73 0.1932002099762613 1101 74-75 0.513070220662641 1101 76-77 0.535673048491681 1101 78-79 0.7265939293209875 1101 80-81 0.6980053209648212 1101 82-83 0.599106036263418 1101 84-85 0.6436318878801011 1101 86-87 0.6246360968555962 1101 88-89 0.6134738760966698 1101 90-91 0.6307024549288442 1101 92-93 0.5011833104588881 1101 94-95 0.6406886043206228 1101 96-97 0.5050113455170617 1101 98-99 0.5149840106227401 1101 100-101 0.5283765152847764 2304 1 0.21486485450261128 2304 2 -0.08649309573338115 2304 3 -0.018006430089020142 2304 4 0.10588257782647759 2304 5 0.0147139768753064 2304 6 0.08010563147934135 2304 7 0.32619334209550743 2304 8 0.03561966011318418 2304 9 0.10258973270538263 2304 10-11 0.021211988912938295 2304 12-13 0.02195254084691811 2304 14-15 0.24516408691415137 2304 16-17 0.15973520065573155 2304 18-19 0.15110883917316897 2304 20-21 0.04349000592669938 2304 22-23 0.07228902583470642 2304 24-25 0.17634395976853767 2304 26-27 0.022001230497785684 2304 28-29 0.0799973720249838 2304 30-31 0.10930191446589532 2304 32-33 0.09986617050467572 2304 34-35 -0.015113975162130089 2304 36-37 0.025830871765997188 2304 38-39 0.10400063488194178 2304 40-41 0.05428883218980474 2304 42-43 0.1569715228968036 2304 44-45 0.03848993383198973 2304 46-47 -0.04423538508543601 2304 48-49 0.13560966399355578 2304 50-51 0.17709912779668002 2304 52-53 0.1909561220005287 2304 54-55 0.27796332985482053 2304 56-57 0.3363159260899593 2304 58-59 0.28456792809470954 2304 60-61 0.2512556100000438 2304 62-63 0.18665630797131882 2304 64-65 0.33555311230575313 2304 66-67 0.4304822298389581 2304 68-69 0.19330590881467202 2304 70-71 0.19998722076784858 2304 72-73 0.14580052167168134 2304 74-75 0.4614629699171857 2304 76-77 0.42620240958120803 2304 78-79 0.3579559601290079 2304 80-81 0.23632993366616617 2304 82-83 0.11348416807768302 2304 84-85 0.32582412936751126 2304 86-87 0.33122778725768853 2304 88-89 0.14819721791450036 2304 90-91 0.2553169476258219 2304 92-93 0.2989268571097874 2304 94-95 0.1835059302866071 2304 96-97 0.2717407095378981 2304 98-99 0.270021783743001 2304 100-101 0.1941080956977963 2202 1 -0.033100877442965526 2202 2 -0.14152124151247492 2202 3 -0.16592037398114456 2202 4 -0.1657434364937913 2202 5 -0.11519930402168654 2202 6 0.10636191946593954 2202 7 0.06722456214311023 2202 8 0.008871153015249433 2202 9 -0.036776706264923575 2202 10-11 -0.059474353612436914 2202 12-13 -0.09083376597756398 2202 14-15 0.0339458130995709 2202 16-17 -0.0873031184520201 2202 18-19 0.026844823046381805 2202 20-21 0.026495015606762706 2202 22-23 0.11851297742675371 2202 24-25 -0.049729876999897726 2202 26-27 -0.005679641642444722 2202 28-29 -0.03577593946462798 2202 30-31 0.20477486424949376 2202 32-33 0.08332595762891515 2202 34-35 0.029767478817113613 2202 36-37 0.18959502137719397 2202 38-39 0.11006445919333174 2202 40-41 -0.021624410590220577 2202 42-43 0.02158808195807893 2202 44-45 0.023803271064060993 2202 46-47 0.05161340700436057 2202 48-49 0.15524725566749709 2202 50-51 0.0703506086934027 2202 52-53 0.09234257417971747 2202 54-55 0.26719393585908335 2202 56-57 0.4160768066459397 2202 58-59 0.22183850409162176 2202 60-61 0.1666853135325681 2202 62-63 0.19384488296547886 2202 64-65 0.17796376140619685 2202 66-67 0.17238541304096344 2202 68-69 0.2796772320091989 2202 70-71 0.4480152304363578 2202 72-73 0.30418912835995116 2202 74-75 0.3746272650031415 2202 76-77 0.3190007637685781 2202 78-79 0.3490563234557449 2202 80-81 0.17122615271807362 2202 82-83 0.11669687321337463 2202 84-85 0.29380745214364623 2202 86-87 0.2725679498310605 2202 88-89 0.18249521066597651 2202 90-91 0.20842264762818274 2202 92-93 0.270250291736712 2202 94-95 0.21066922202592764 2202 96-97 0.2747187852282913 2202 98-99 0.13888673397559614 2202 100-101 0.37697972600402707 1102 1 5.640428139663811E-4 1102 2 -0.30059212482520437 1102 3 -0.12079361390367538 1102 4 -0.21026923352798832 1102 5 -0.17478339864442916 1102 6 0.07246982961417814 1102 7 -0.08055391954044921 1102 8 0.20003429750985902 1102 9 -0.0863396030292094 1102 10-11 -0.11143226894954239 1102 12-13 -0.07805439815173543 1102 14-15 -0.13406133502196838 1102 16-17 -0.19886070893520724 1102 18-19 -0.19397939498522732 1102 20-21 -0.17580180393544254 1102 22-23 0.04810813978554762 1102 24-25 -0.12742841668752902 1102 26-27 -0.0019829538113071976 1102 28-29 -0.11634108677986177 1102 30-31 -0.03970204889269979 1102 32-33 -0.1594771621483062 1102 34-35 -0.16277746334159104 1102 36-37 -0.0992696203222394 1102 38-39 -0.09756152533576312 1102 40-41 -0.09038021676962416 1102 42-43 -0.11431414580636101 1102 44-45 -0.024104334206718647 1102 46-47 -0.06506043783199544 1102 48-49 -0.03009405934536602 1102 50-51 0.04039308787740481 1102 52-53 -0.03869018359238652 1102 54-55 0.04517676445301788 1102 56-57 0.17109902141177002 1102 58-59 -0.01646615690540898 1102 60-61 0.02194072143483794 1102 62-63 0.03348415489875123 1102 64-65 -0.04484460845094418 1102 66-67 0.07927446663824611 1102 68-69 0.14432296568629965 1102 70-71 0.019529415250854498 1102 72-73 0.14262730887756447 1102 74-75 0.2718939543172141 1102 76-77 0.23648401679265874 1102 78-79 0.20552249104485654 1102 80-81 0.09139544623315388 1102 82-83 0.0023203208994004854 1102 84-85 0.036406842666959705 1102 86-87 -0.11940588267998464 1102 88-89 -0.022589757439860136 1102 90-91 -0.08162993942034191 1102 92-93 -0.11666508738054304 1102 94-95 -0.08909364797325026 1102 96-97 0.04827904053742671 1102 98-99 0.08942643217529778 1102 100-101 0.02665917064740242 2305 1 0.1649745309244075 2305 2 -0.018656197807143116 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1213 58-59 -0.04270423467503548 1213 60-61 0.1256641256901645 1213 62-63 0.150656752110784 1213 64-65 0.2708835646965255 1213 66-67 0.3241937623095552 1213 68-69 0.4204143083128642 1213 70-71 0.2661682633799849 1213 72-73 0.5793523639032472 1213 74-75 0.5553908361954285 1213 76-77 0.3811052567046218 1213 78-79 0.32544637218939343 1213 80-81 0.13660362671738113 1213 82-83 0.17223503109749672 1213 84-85 0.26274580084744414 1213 86-87 0.23756403661568726 1213 88-89 0.19269362480518737 1213 90-91 0.2537148881658666 1213 92-93 0.15389158906846845 1213 94-95 0.3219665134354095 1213 96-97 0.38282141030998673 1213 98-99 0.20088552975416363 1213 100-101 0.27461863506412953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1407.0 3 509.0 4 558.0 5 384.0 6 996.0 7 5818.0 8 32649.0 9 89386.0 10 100972.0 11 58192.0 12 28613.0 13 17175.0 14 11753.0 15 9430.0 16 8336.0 17 7838.0 18 7926.0 19 8434.0 20 9188.0 21 9594.0 22 10793.0 23 11710.0 24 13099.0 25 15341.0 26 18123.0 27 21889.0 28 27199.0 29 33685.0 30 40963.0 31 51851.0 32 71072.0 33 101568.0 34 154576.0 35 261001.0 36 507492.0 37 872825.0 38 288516.0 39 402.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.16043455403228 22.015438285100466 23.524367699353764 13.299759461513498 2 32.07970103501128 25.447844731086505 30.969810735874077 11.502643498028139 3 36.51110708626388 20.33564012172791 28.73786835673522 14.415384435272996 4 27.2230846535763 18.16690129619473 35.70569091079981 18.90432313942916 5 34.2292867090972 16.30313787398579 32.30280044966955 17.16477496724746 6 31.086189494447698 16.37159568892509 37.61622430969152 14.92599050693569 7 36.91199322424095 18.234140041756785 28.711487725645057 16.142379008357207 8 27.727304313365565 32.53136139375105 24.271376075967744 15.469958216915646 9 33.15642523428773 27.922749709719845 22.548508427754633 16.372316628237794 10-11 26.989611642773113 30.754183248984603 26.66732462919971 15.588880479042574 12-13 30.000209814647675 23.938062716005902 26.60791971184538 19.453807757501046 14-15 26.532427551810127 24.274451801530656 28.55237767649841 20.640742970160805 16-17 27.908708877686916 25.181793230020798 29.670440224748777 17.239057667543502 18-19 27.897939739216245 25.28998182997763 30.171247345297314 16.640831085508808 20-21 26.04404706979665 28.21398594977128 29.131551555823894 16.610415424608174 22-23 29.559094778599597 25.84265256298382 27.938027875380772 16.66022478303581 24-25 25.46721412989913 29.827948434449418 27.878806826788892 16.826030608862556 26-27 22.477748816821975 33.036754172667045 27.759196633567175 16.726300376943804 28-29 24.479707857859495 30.88506540328729 27.58611016938944 17.04911656946378 30-31 27.910404747250233 27.258687963695554 27.728961750893045 17.101945538161168 32-33 27.718599964916063 26.742367090304597 28.615256410830803 16.92377653394853 34-35 23.912063066160357 26.691017001289897 28.26949771618677 21.127422216362977 36-37 19.77978785566107 26.947193783964433 29.10387566550348 24.169142694871017 38-39 19.505042614456396 28.601152917630202 30.92597886932319 20.96782559859021 40-41 21.03675953963848 26.873844171730294 33.979606239003964 18.109790049627268 42-43 22.90282199846502 26.35561768714119 33.192112475252166 17.549447839141624 44-45 21.40838895995211 28.37790729032813 32.808070545414566 17.405633204305197 46-47 24.00468872479233 30.084967194093625 28.491983114799645 17.4183609663144 48-49 20.6191920797021 29.6833307883243 30.728881394421364 18.968595737552235 50-51 19.379668327282403 27.25711943609847 32.82257219403971 20.54064004257942 52-53 25.035004562440815 26.60432910578745 28.57080572101644 19.789860610755298 54-55 27.622178553039788 26.72281702528317 27.78674213730703 17.868262284370008 56-57 28.69053043436901 26.437688833987394 27.128330940201106 17.74344979144249 58-59 26.6571283036763 26.367117495905728 27.806022575006477 19.169731625411497 60-61 21.956191190896302 26.5920601400341 27.456617922449595 23.99513074662 62-63 24.32871917482585 26.69647840256868 27.41058201787326 21.56422040473221 64-65 21.625258430016867 28.20647457958232 30.247281413556422 19.920985576844394 66-67 19.629678831339543 28.422458976255623 29.182619651044245 22.765242541360596 68-69 18.964100390729982 27.38507232294832 34.52552493573973 19.12530235058196 70-71 18.362306813323702 31.56305904554422 31.86824430115884 18.206389839973237 72-73 18.19876662204496 35.953566785466506 27.393708821663136 18.453957770825397 74-75 18.596762801617615 36.55170739041633 26.57777727258088 18.273752535385167 76-77 18.77983555856919 36.17451411000067 26.78872123097357 18.256929100456563 78-79 19.066561046200686 35.794779365701906 26.922815243589355 18.215844344508053 80-81 18.57215428335283 35.86020450507886 26.813630754967537 18.754010456600767 82-83 18.39897997863469 35.15841345325477 27.02312278162583 19.41948378648472 84-85 18.600929435726318 34.07814992190383 27.589414342474356 19.731506299895493 86-87 18.772007626151787 33.74048410833601 27.91598139152805 19.571526873984148 88-89 19.25880094406039 33.919166982835236 27.38569429411361 19.43633777899076 90-91 19.76462898173642 33.28166459147495 27.004334557773973 19.949371869014655 92-93 19.618937375814873 33.25159831155599 27.24969833834165 19.879765974287487 94-95 19.16413576997594 33.24351176384667 27.379898387483497 20.21245407869389 96-97 19.41732519497434 33.885997508876564 26.9322314505195 19.764445845629595 98-99 19.920711494593498 33.02526481189224 26.810162436338324 20.243861257175936 100-101 20.148717433602567 32.84388580549332 26.323154595938302 20.68424216496581 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 902.0 1 706.5 2 406.5 3 361.5 4 416.0 5 434.5 6 544.0 7 704.5 8 874.5 9 1028.0 10 1150.0 11 1202.5 12 1152.5 13 1055.5 14 1008.0 15 982.0 16 921.5 17 919.5 18 951.0 19 1041.0 20 1208.0 21 1501.0 22 1977.0 23 2715.0 24 3790.0 25 5439.5 26 8430.5 27 12561.5 28 17063.5 29 21534.0 30 26440.5 31 31323.5 32 37797.0 33 49068.0 34 65589.5 35 88710.5 36 114715.0 37 142832.5 38 174982.0 39 190663.5 40 185211.0 41 169737.5 42 154574.5 43 151571.0 44 145645.5 45 137648.0 46 140386.5 47 133300.0 48 110934.0 49 92295.0 50 80212.5 51 70196.5 52 61729.0 53 53469.0 54 45320.0 55 37284.5 56 29291.5 57 22846.5 58 17673.0 59 13703.0 60 10633.0 61 8092.5 62 6091.5 63 4575.5 64 3400.5 65 2578.0 66 1915.0 67 1410.5 68 1091.0 69 855.0 70 646.0 71 461.5 72 358.5 73 315.0 74 236.0 75 167.0 76 130.0 77 103.5 78 88.5 79 81.5 80 70.0 81 54.0 82 42.0 83 36.0 84 30.5 85 18.0 86 15.0 87 17.0 88 13.0 89 14.0 90 11.5 91 5.5 92 6.0 93 7.0 94 5.0 95 2.5 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29571357860832226 2 0.27180642903097385 3 0.24322776746724703 4 0.229591074389363 5 0.23728532942575098 6 0.18383773640512727 7 0.15216763308570885 8 0.14924793809422235 9 0.12802003803847334 10-11 0.11321546696399466 12-13 0.13516470342940504 14-15 0.13784395295100443 16-17 0.13186716555666733 18-19 0.11855679133077293 20-21 0.12446488001942799 22-23 0.15472665987236467 24-25 0.5694607460748136 26-27 0.22622483781094324 28-29 0.20018802835745172 30-31 0.2987878456875933 32-33 0.33147125491582174 34-35 0.33887354045306106 36-37 0.3299770580672375 38-39 0.33804915598487667 40-41 0.3412436457990913 42-43 0.352132390649694 44-45 0.33317154788145215 46-47 0.3524587095016836 48-49 0.3489894248647408 50-51 0.3548631642005549 52-53 0.33853004692465094 54-55 0.3314369055629807 56-57 0.3402303398902813 58-59 0.33136820685729873 60-61 0.30811369498392965 62-63 0.2955074824912761 64-65 0.30507377725749957 66-67 0.3220767069138034 68-69 0.30924722362768325 70-71 0.2897711405668261 72-73 0.28650795204692947 74-75 0.2783843301000287 76-77 0.2803765925648078 78-79 0.2891013281864263 80-81 0.2986504482762292 82-83 0.3216129906504496 84-85 0.31161732897371347 86-87 0.31409048237826676 88-89 0.3204794620066961 90-91 0.3133176219393438 92-93 0.2950781155807634 94-95 0.29097336791626177 96-97 0.30770150274983743 98-99 0.3161170941958868 100-101 0.3177143391029941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2911263.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.45712295552027 #Duplication Level Percentage of deduplicated Percentage of total 1 92.54265769926397 57.799481505535546 2 3.631790163572282 4.536623295897662 3 1.103870014420627 2.068336356527431 4 0.5822561610489357 1.4546417856897038 5 0.3582695199154022 1.1188241728285746 6 0.2149070760537252 0.8053486603859317 7 0.19784745882154625 0.8649888143438182 8 0.16634462033807765 0.8311525124355724 9 0.12722457403633994 0.7151472777196238 >10 0.9061209426607226 10.907529752329982 >50 0.08740716625163064 3.834651593580994 >100 0.07074134953985173 9.321050543500158 >500 0.008537841811987423 3.53848188901898 >1k 0.0019703044844411027 1.9443278402426445 >5k 5.5107780523350934E-5 0.25941399996331915 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT 7537 0.25889107236275116 Illumina Single End PCR Primer 1 (96% over 32bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3537 0.12149366099867996 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3474 0.11932965176969584 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.005186752278993688 0.0 0.0 2 0.005667643218767937 0.0 0.0 3 0.0067324731568394885 0.0 0.0 4 0.007213364096613738 0.0 0.0 5 0.0081064472704802 0.0 0.0 6 0.009068229150028698 0.0 0.0 7 0.0106139500278745 0.0 0.0 8 0.011472683848899944 0.0 0.0 9 0.012777959256858621 0.0 0.0 10-11 0.019149764208867423 0.0 0.0 12-13 0.024405215193543148 0.0 0.0 14-15 0.029901111648105994 0.0 0.0 16-17 0.034400876870279325 0.0 0.0 18-19 0.0391754369151808 0.0 0.0 20-21 0.044619809340482125 0.0 0.0 22-23 0.05054507270555769 0.0 0.0 24-25 0.05647033607063326 0.0 0.0 26-27 0.06303106246326766 0.0 3.4349352841017796E-5 28-29 0.0708283655581787 0.0 3.4349352841017796E-5 30-31 0.13701956848282 0.0 5.152402926152669E-5 32-33 0.14713545289449975 0.0 6.869870568203559E-5 34-35 0.1557056164283337 0.0 6.869870568203559E-5 36-37 0.16522038716529563 0.0 6.869870568203559E-5 38-39 0.17573128913464708 0.0 1.030480585230534E-4 40-41 0.18600174563411137 0.0 1.030480585230534E-4 42-43 0.19795532042278557 0.0 1.030480585230534E-4 44-45 0.21038978615123402 0.0 1.030480585230534E-4 46-47 0.223854732464913 0.0 1.030480585230534E-4 48-49 0.23817841259961742 0.0 1.030480585230534E-4 50-51 0.2546489272868855 0.0 1.3739741136407118E-4 52-53 0.2724934160877942 0.0 1.3739741136407118E-4 54-55 0.2921412459128564 0.0 1.3739741136407118E-4 56-57 0.3128710803524106 0.0 1.3739741136407118E-4 58-59 0.3356103519331644 0.0 1.3739741136407118E-4 60-61 0.3598438203625025 0.0 1.3739741136407118E-4 62-63 0.38550278693474277 0.0 1.7174676420508899E-4 64-65 0.41305096791323903 0.0 1.889214406255979E-4 66-67 0.44308947697270906 0.0 2.060961170461068E-4 68-69 0.4739695451767841 0.0 2.060961170461068E-4 70-71 0.5094867760143965 0.0 2.404454698871246E-4 72-73 0.5464123303184907 0.0 2.404454698871246E-4 74-75 0.5869102173180506 0.0 2.404454698871246E-4 76-77 0.6301388778684716 0.0 2.404454698871246E-4 78-79 0.6781249237873734 0.0 2.404454698871246E-4 80-81 0.7322423291883968 0.0 2.404454698871246E-4 82-83 0.7913403907513681 0.0 2.404454698871246E-4 84-85 0.8546290733609434 0.0 2.404454698871246E-4 86-87 0.9252513428020759 0.0 2.404454698871246E-4 88-89 1.0010431898457819 0.0 2.404454698871246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2875 0.0 86.06576 9 CGGAAAG 2235 0.0 84.48741 5 CGGGAGA 3805 0.0 83.66846 4 AAGCGGC 1235 0.0 80.98662 9 GTTCGGA 825 0.0 79.38915 2 GAGAGCG 3525 0.0 78.80589 7 ATTCGGA 925 0.0 78.50291 2 TCGGGAG 4000 0.0 77.45725 3 ATCGGGA 4860 0.0 77.44136 2 GATCGGG 5155 0.0 74.86137 1 TCGGAGA 1375 0.0 73.84226 4 ATCCGGA 940 0.0 73.71587 2 GATCCGG 970 0.0 72.42456 1 AGAGCGG 3625 0.0 71.26909 8 TCGGGAA 1605 0.0 71.24443 3 AAAGCGG 1620 0.0 71.10563 8 GGTTCGG 1085 0.0 70.873055 1 TTCGGAG 925 0.0 70.27287 3 CGGAGAG 2275 0.0 69.44633 5 GGATCGG 2115 0.0 69.3496 1 >>END_MODULE