FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_34012-1.34200000035798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_34012-1.34200000035798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2911263
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT75370.25889107236275116Illumina Single End PCR Primer 1 (96% over 32bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC35370.12149366099867996No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG34740.11932965176969584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC28750.086.065769
CGGAAAG22350.084.487415
CGGGAGA38050.083.668464
AAGCGGC12350.080.986629
GTTCGGA8250.079.389152
GAGAGCG35250.078.805897
ATTCGGA9250.078.502912
TCGGGAG40000.077.457253
ATCGGGA48600.077.441362
GATCGGG51550.074.861371
TCGGAGA13750.073.842264
ATCCGGA9400.073.715872
GATCCGG9700.072.424561
AGAGCGG36250.071.269098
TCGGGAA16050.071.244433
AAAGCGG16200.071.105638
GGTTCGG10850.070.8730551
TTCGGAG9250.070.272873
CGGAGAG22750.069.446335
GGATCGG21150.069.34961
Produced by FastQC (version 0.11.2)