FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_30749-1.3420000003587d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_30749-1.3420000003587d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5141247
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT178320.34684192375896356Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC149050.088.537149
ATCGGGA232600.086.504892
TCGGGAG214900.084.994093
GAGAGCG183500.082.858137
TCCGGAG40600.082.562283
GATCGGG255750.080.5471951
GGATCGG76150.080.213541
CGGAGAG110200.079.919475
AGAGCGG171500.079.8052448
CGGGAGA235100.077.083214
CCGGAGA53200.076.839014
TCCGGAA28150.076.4051743
ATTGGGA104850.075.909742
TCGGAGA63400.075.6349264
CGGAAAG97050.075.631775
GGAGAGC204500.075.600686
GAGCGTC220300.075.249089
ATCCGGA50050.074.672822
ACCGGAA26550.073.6776353
GAGGGGC231800.071.986349
Produced by FastQC (version 0.11.2)