##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_30749-1.3420000003587d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5141247 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.154218227601202 33.0 22.0 33.0 15.0 33.0 2 26.34444503444398 33.0 22.0 33.0 6.0 33.0 3 25.843982014480144 33.0 15.0 33.0 6.0 33.0 4 28.33429963586655 37.0 15.0 37.0 6.0 37.0 5 28.53807121112835 37.0 15.0 37.0 6.0 37.0 6 30.449301502145296 37.0 27.0 37.0 15.0 37.0 7 28.941795443790195 37.0 15.0 37.0 6.0 37.0 8 29.52575357690459 37.0 22.0 37.0 6.0 37.0 9 27.983515088849067 37.0 15.0 37.0 6.0 37.0 10-11 28.258928719044235 37.0 15.0 37.0 6.0 37.0 12-13 28.231145965171486 37.0 15.0 37.0 6.0 37.0 14-15 29.405474586223924 37.0 15.0 40.0 6.0 40.0 16-17 29.19270208181011 37.0 15.0 40.0 6.0 40.0 18-19 29.39363436535922 37.0 15.0 40.0 6.0 40.0 20-21 29.303757434723522 37.0 15.0 40.0 6.0 40.0 22-23 32.006664822756036 37.0 24.5 40.0 15.0 40.0 24-25 30.28973690624084 37.0 18.5 40.0 6.0 40.0 26-27 29.669774278497027 37.0 18.5 40.0 6.0 40.0 28-29 28.92678147927925 37.0 15.0 40.0 6.0 40.0 30-31 29.025978716836597 37.0 15.0 40.0 6.0 40.0 32-33 28.730210394482118 37.0 15.0 40.0 6.0 40.0 34-35 28.814127875980283 37.0 15.0 40.0 6.0 40.0 36-37 28.53674060009177 37.0 15.0 40.0 6.0 40.0 38-39 28.536709770995245 37.0 10.5 40.0 6.0 40.0 40-41 28.686416738974028 37.0 15.0 40.0 6.0 40.0 42-43 28.02961120132917 37.0 6.0 40.0 6.0 40.0 44-45 27.83964026626225 37.0 6.0 40.0 6.0 40.0 46-47 27.842743890733125 37.0 6.0 40.0 6.0 40.0 48-49 27.809021235509597 37.0 10.5 40.0 6.0 40.0 50-51 27.333831169753175 35.0 15.0 38.5 6.0 38.5 52-53 27.704847676059913 37.0 15.0 37.0 6.0 40.0 54-55 28.13099166408461 37.0 15.0 40.0 6.0 40.0 56-57 28.820173199225792 37.0 18.5 40.0 6.0 40.0 58-59 27.807071319467823 37.0 10.5 40.0 6.0 40.0 60-61 27.428424563145867 37.0 6.0 38.5 6.0 40.0 62-63 27.03286965205134 37.0 6.0 37.0 6.0 40.0 64-65 26.868659587839293 37.0 6.0 37.0 6.0 40.0 66-67 26.933612895859703 35.0 10.5 37.0 6.0 40.0 68-69 27.45581995963236 33.0 15.0 37.0 6.0 40.0 70-71 27.04818043171238 33.0 15.0 37.0 6.0 38.5 72-73 27.871463187821945 33.0 15.0 37.0 6.0 37.0 74-75 28.727079733768868 33.0 22.0 37.0 10.5 37.0 76-77 28.573402328267832 33.0 22.0 37.0 10.5 37.0 78-79 27.95894906430288 33.0 22.0 37.0 6.0 37.0 80-81 27.13676594413768 33.0 15.0 37.0 6.0 37.0 82-83 26.606018053596724 33.0 15.0 37.0 6.0 37.0 84-85 26.21833000826453 33.0 15.0 37.0 6.0 37.0 86-87 25.977183648247205 33.0 15.0 37.0 6.0 37.0 88-89 25.787630121641694 33.0 15.0 37.0 6.0 37.0 90-91 25.623143373582323 33.0 15.0 37.0 6.0 37.0 92-93 25.457023558681385 33.0 15.0 37.0 6.0 37.0 94-95 25.324343490985747 33.0 15.0 37.0 6.0 37.0 96-97 25.202860025982027 33.0 15.0 37.0 6.0 37.0 98-99 25.070604855203417 33.0 15.0 37.0 6.0 37.0 100-101 24.07145990068168 30.0 15.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.5370062078746685 2306 2 -0.21275208430302683 2306 3 -0.47404665595301765 2306 4 -0.15159489656840108 2306 5 -0.4901041220306297 2306 6 -1.2140775377076736 2306 7 0.07704801234464398 2306 8 -1.2391849485891413 2306 9 -0.21628011191231167 2306 10-11 -0.3069568436498855 2306 12-13 -0.3180556226005642 2306 14-15 0.33907110226097714 2306 16-17 0.15229302873848383 2306 18-19 0.15573375904240194 2306 20-21 0.1775640158442009 2306 22-23 -0.28180550198354126 2306 24-25 0.41656580684642464 2306 26-27 -0.8413056687571476 2306 28-29 -0.2941682558420631 2306 30-31 -0.2665921681567802 2306 32-33 -0.12616148417930972 2306 34-35 -0.4759687819978353 2306 36-37 -0.2776304156037561 2306 38-39 -0.21532807235703189 2306 40-41 -0.44464853372126356 2306 42-43 0.052250465927787815 2306 44-45 0.3437241818109449 2306 46-47 0.20779437129570155 2306 48-49 0.01583879943593658 2306 50-51 -0.10660802976644845 2306 52-53 -0.15703216627447603 2306 54-55 0.12322582667370341 2306 56-57 -0.07358431038356983 2306 58-59 0.45638208559346083 2306 60-61 0.21529298768604477 2306 62-63 0.27566902901840606 2306 64-65 0.19936117102154327 2306 66-67 -0.07154410818537116 2306 68-69 -0.46997029629756426 2306 70-71 -0.13203047222948427 2306 72-73 -0.7494177061419336 2306 74-75 -1.0643121279186794 2306 76-77 -0.8555715915153854 2306 78-79 -0.705257919245998 2306 80-81 -0.49277658194132457 2306 82-83 -0.31167571455580045 2306 84-85 -0.10671528576794742 2306 86-87 -0.08761086488604519 2306 88-89 -0.11912749677339818 2306 90-91 -0.17431896671039127 2306 92-93 0.05788077355597565 2306 94-95 -0.04257071673157853 2306 96-97 -0.0686673383771037 2306 98-99 -0.011141784469753446 2306 100-101 -0.0890385476198432 2307 1 -0.5539294654149707 2307 2 -0.24884852404930413 2307 3 -0.5412854471854587 2307 4 -0.24056100834599548 2307 5 -0.30121346947451144 2307 6 -1.0932071399235035 2307 7 0.32700136599572716 2307 8 -1.0548419838917802 2307 9 -0.21858786669656283 2307 10-11 -0.3902859570498265 2307 12-13 -0.46089011136301394 2307 14-15 0.23316167406687072 2307 16-17 0.04212108935019998 2307 18-19 0.049944797646350736 2307 20-21 -0.12118811768221249 2307 22-23 -0.529647072286231 2307 24-25 0.2300970554342605 2307 26-27 -0.9806583150792676 2307 28-29 -0.5093441733218143 2307 30-31 -0.20740591816065646 2307 32-33 -0.13495881527392228 2307 34-35 -0.5126557474902889 2307 36-37 -0.30325252651056545 2307 38-39 -0.1436587295335059 2307 40-41 -0.4890299327393173 2307 42-43 -0.10066179147618826 2307 44-45 0.09693854102938815 2307 46-47 0.07558771854611379 2307 48-49 0.012800447394482717 2307 50-51 -0.25022356575210836 2307 52-53 -0.2067816233412536 2307 54-55 -0.0047615739298336734 2307 56-57 -0.19644159662653848 2307 58-59 0.31718722314852954 2307 60-61 0.20027248486527682 2307 62-63 0.2996580446264083 2307 64-65 0.30303346681442633 2307 66-67 0.17912871572365674 2307 68-69 -0.28796911078012855 2307 70-71 -0.0693113242760468 2307 72-73 -0.5606241688242122 2307 74-75 -0.8271447182788059 2307 76-77 -0.7419904379574191 2307 78-79 -0.48399235403114815 2307 80-81 -0.2257115687013318 2307 82-83 -0.10180196360877858 2307 84-85 -0.014073252460978125 2307 86-87 -0.1408058313343581 2307 88-89 -0.040524443842716806 2307 90-91 0.15340317985580398 2307 92-93 0.05555091616096064 2307 94-95 0.1520098820659932 2307 96-97 -0.016276695017790388 2307 98-99 -0.029788629333420857 2307 100-101 -0.004592533284483835 2201 1 -0.4142142345793616 2201 2 -0.5061807707214037 2201 3 -0.6052527195059589 2201 4 -0.3954888572361632 2201 5 -0.5502225303115615 2201 6 -0.9510424468254008 2201 7 0.2601525237693494 2201 8 -1.1300696234398586 2201 9 -0.45822607242710234 2201 10-11 -0.5789754116417711 2201 12-13 -0.5571082106646728 2201 14-15 0.11341831788169188 2201 16-17 -0.2422341849518581 2201 18-19 -0.22022123779798264 2201 20-21 -0.35692520958498974 2201 22-23 -0.5517229148481952 2201 24-25 -0.07660380910063225 2201 26-27 -1.0070398929752997 2201 28-29 -0.5296634599606769 2201 30-31 -0.23826581876311437 2201 32-33 -0.2827866114290565 2201 34-35 -0.5725432460920068 2201 36-37 -0.44752054830771115 2201 38-39 -0.3614425798299372 2201 40-41 -0.5387855937649348 2201 42-43 -0.211629399383245 2201 44-45 -0.07372576055106705 2201 46-47 -0.15302982464067938 2201 48-49 -0.2305308084982549 2201 50-51 -0.36479249682902193 2201 52-53 -0.2766859012708416 2201 54-55 -0.12202766210919691 2201 56-57 -0.054886073031607197 2201 58-59 0.2247117129770615 2201 60-61 -0.012551715719606449 2201 62-63 -0.0019847314734349197 2201 64-65 0.05674617276069682 2201 66-67 -0.16637362539209022 2201 68-69 -0.42953454216866405 2201 70-71 -0.2193418466724566 2201 72-73 -0.5442401938813433 2201 74-75 -0.5828255594824405 2201 76-77 -0.5449442388746917 2201 78-79 -0.5081424411238515 2201 80-81 -0.2648946551388427 2201 82-83 -0.16872974857769307 2201 84-85 -0.05182578134539284 2201 86-87 -0.07508264230324357 2201 88-89 0.03866087983850619 2201 90-91 -0.041225837195188575 2201 92-93 0.09327723733327886 2201 94-95 -0.04202147130665068 2201 96-97 -0.05403082193343067 2201 98-99 -0.10756353065499269 2201 100-101 -0.1885281645867174 1101 1 -0.3235769796774015 1101 2 -0.5709585484991848 1101 3 -0.6126661181987707 1101 4 -0.4820542167132871 1101 5 -0.5932944257363957 1101 6 -0.5167630350606949 1101 7 0.2603012165798084 1101 8 -0.7581039371653482 1101 9 -0.3858110397473631 1101 10-11 -0.44401626131497096 1101 12-13 -0.4344333740633637 1101 14-15 -0.02845178015752481 1101 16-17 -0.30188532874270635 1101 18-19 -0.22113218551040248 1101 20-21 -0.38177406579413997 1101 22-23 -0.2625644181161668 1101 24-25 -0.21695021432939043 1101 26-27 -0.6666273112759562 1101 28-29 -0.3438807802221113 1101 30-31 -0.18220536124677622 1101 32-33 -0.35698187286696736 1101 34-35 -0.40626465132076817 1101 36-37 -0.26363574438614634 1101 38-39 -0.38638947525477363 1101 40-41 -0.5871369009544765 1101 42-43 -0.20748887650742986 1101 44-45 -0.14209585859028095 1101 46-47 -0.23334355013087915 1101 48-49 -0.26113048170087083 1101 50-51 -0.2618774641492827 1101 52-53 -0.2952251169571163 1101 54-55 0.05109406991694598 1101 56-57 -0.0340535566917346 1101 58-59 0.1490148175522279 1101 60-61 -0.10109256539280764 1101 62-63 0.05332736002983296 1101 64-65 0.02174944073455265 1101 66-67 -0.11134666460777254 1101 68-69 -0.336661993149054 1101 70-71 -0.08469151987507217 1101 72-73 -0.4498415010708854 1101 74-75 -0.4315976183059753 1101 76-77 -0.34302021926684745 1101 78-79 -0.34465959798659895 1101 80-81 -0.3056373021976633 1101 82-83 -0.22598791851233457 1101 84-85 -0.13424980095323846 1101 86-87 -0.08242251158428715 1101 88-89 -0.013303172449074196 1101 90-91 -0.2053328633389775 1101 92-93 -0.05399645547717924 1101 94-95 -0.07812849745044304 1101 96-97 -0.1686623578811428 1101 98-99 -0.1417400012432246 1101 100-101 -0.18528551752789468 2304 1 -0.12770441181768533 2304 2 -0.568003708239754 2304 3 -0.468496829224474 2304 4 -0.4762196022525629 2304 5 -0.38660453863574773 2304 6 -0.197068643996559 2304 7 0.518146460394366 2304 8 -0.2979449772806504 2304 9 -0.24592021582230927 2304 10-11 -0.2461196122638043 2304 12-13 -0.25542346534369287 2304 14-15 -0.015260001392210398 2304 16-17 -0.19095502007107612 2304 18-19 -0.06043001760264488 2304 20-21 -0.19691924355056756 2304 22-23 -0.12621893409022533 2304 24-25 0.03559914911043549 2304 26-27 -0.30564829974581187 2304 28-29 -0.09424163777676142 2304 30-31 0.16743918595476615 2304 32-33 -0.005144105631796236 2304 34-35 -0.1944166751016958 2304 36-37 -0.0725207131586636 2304 38-39 -0.0848339563981817 2304 40-41 -0.28455336744498894 2304 42-43 0.055074539298477276 2304 44-45 -0.03338654439378885 2304 46-47 -0.19069449896585056 2304 48-49 0.008972629036577473 2304 50-51 0.023770866381585876 2304 52-53 -0.0115014801567348 2304 54-55 0.10043495990013085 2304 56-57 0.19280901236903603 2304 58-59 0.19193287544317172 2304 60-61 0.026242109120769186 2304 62-63 0.03434339413984944 2304 64-65 0.1505266446740201 2304 66-67 0.13152617348975326 2304 68-69 -0.050153345539154515 2304 70-71 0.06961631101611587 2304 72-73 -0.12043794594237056 2304 74-75 0.21330590679611916 2304 76-77 0.16698983958494296 2304 78-79 0.18923624035114273 2304 80-81 -0.030729813607702994 2304 82-83 0.014032619561167081 2304 84-85 0.0715079758903876 2304 86-87 0.1545683603830419 2304 88-89 0.06323189841328514 2304 90-91 0.09216464446788919 2304 92-93 0.1294461640279856 2304 94-95 0.12737669712077704 2304 96-97 -0.002168273086521566 2304 98-99 0.027642415283317234 2304 100-101 0.1378162420205058 2202 1 -0.0314870024564442 2202 2 -0.5774437421210514 2202 3 -0.35340017286847214 2202 4 -0.3942558112463175 2202 5 -0.43167711366350403 2202 6 0.051348012775296326 2202 7 0.2918883464203539 2202 8 -0.11171954080999313 2202 9 -0.34692379601160184 2202 10-11 -0.3075270813605293 2202 12-13 -0.26696811210436877 2202 14-15 -0.18700331774294554 2202 16-17 -0.2630985975354214 2202 18-19 -0.20304420145573232 2202 20-21 -0.2598876762724984 2202 22-23 -0.019554737022776436 2202 24-25 -0.11333062435216945 2202 26-27 -0.029786479840876012 2202 28-29 -0.18002941347951662 2202 30-31 0.07798427428853572 2202 32-33 -0.16504992525876716 2202 34-35 -0.0346231365143872 2202 36-37 -0.13446860315607623 2202 38-39 -0.14269215179236028 2202 40-41 -0.11292676428021764 2202 42-43 -0.006168830949345505 2202 44-45 -0.048498193442217286 2202 46-47 -0.034154484071653 2202 48-49 0.024929313287739063 2202 50-51 -0.027909354809874998 2202 52-53 -0.03201441080677725 2202 54-55 0.16601348905134472 2202 56-57 0.29477417679345663 2202 58-59 0.022755990672955306 2202 60-61 0.007780441440907282 2202 62-63 0.14751130080432162 2202 64-65 0.10790775508534978 2202 66-67 0.14672879097309277 2202 68-69 0.12730497723819312 2202 70-71 0.13267863957823067 2202 72-73 0.2433726219359471 2202 74-75 0.4034681561814999 2202 76-77 0.34509908597233263 2202 78-79 0.19718294187672925 2202 80-81 -0.008738953678300021 2202 82-83 0.19346880130543198 2202 84-85 0.20359786419482973 2202 86-87 0.06354331985079043 2202 88-89 0.17114215784705422 2202 90-91 0.05919475625099935 2202 92-93 0.07475325978478153 2202 94-95 0.055437846513111566 2202 96-97 0.10209703847420926 2202 98-99 0.027774805134907155 2202 100-101 0.07091977859966647 1102 1 -0.06922344759703947 1102 2 -0.5893895271122744 1102 3 -0.3777027891860243 1102 4 -0.43340904496993105 1102 5 -0.4327089861166087 1102 6 0.24244159876950633 1102 7 -0.02311112949925942 1102 8 0.3170181734416957 1102 9 -0.22342467120124354 1102 10-11 -0.22185029674378853 1102 12-13 -0.17938050087261104 1102 14-15 -0.33620083502898623 1102 16-17 -0.303121853910131 1102 18-19 -0.265996762092815 1102 20-21 -0.3191224401730537 1102 22-23 0.04105272381513103 1102 24-25 -0.25719022297056426 1102 26-27 0.15191435539071207 1102 28-29 -0.12596368149668535 1102 30-31 0.04813720734255256 1102 32-33 -0.06643005281943815 1102 34-35 -0.07840077683383484 1102 36-37 -0.18765321576224991 1102 38-39 -0.30462465023857277 1102 40-41 -0.14107711482356322 1102 42-43 -0.1933768652739012 1102 44-45 -0.25137256522238616 1102 46-47 -0.17229710381418073 1102 48-49 -0.11613528835457743 1102 50-51 -0.024335446434871955 1102 52-53 -0.012054993820370896 1102 54-55 0.016393459633945895 1102 56-57 0.2298121000375808 1102 58-59 -0.05176912606410511 1102 60-61 -0.08379989959025735 1102 62-63 -0.010819485941219398 1102 64-65 -0.08552891312157129 1102 66-67 0.014862316813697873 1102 68-69 0.1843785123596966 1102 70-71 0.09931809343234121 1102 72-73 0.30850392990772946 1102 74-75 0.41002208897421966 1102 76-77 0.2785401879485363 1102 78-79 0.22997479929143338 1102 80-81 0.14889869369777742 1102 82-83 0.01788905275575914 1102 84-85 0.08704883266093688 1102 86-87 0.1337837964736046 1102 88-89 0.12234956032257926 1102 90-91 -0.07552537252022873 1102 92-93 0.0017947251233820793 1102 94-95 0.0482631368118156 1102 96-97 -0.005112536105382759 1102 98-99 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0.5003772523748502 1213 62-63 0.5708313463624073 1213 64-65 0.6565837231946894 1213 66-67 0.8907777659161447 1213 68-69 1.0972793508144996 1213 70-71 0.8749907124711704 1213 72-73 1.2932587267195501 1213 74-75 1.6056036914890193 1213 76-77 1.3633080836944274 1213 78-79 1.3828179077081657 1213 80-81 1.2673003401713565 1213 82-83 1.2238361133783222 1213 84-85 1.0027009393656705 1213 86-87 0.8864932059555741 1213 88-89 0.7459855519245018 1213 90-91 0.7776884920140965 1213 92-93 0.7470624852973096 1213 94-95 0.788880329428828 1213 96-97 0.7312826876769414 1213 98-99 0.729098837025564 1213 100-101 0.8161788902169818 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2502.0 3 1587.0 4 1680.0 5 917.0 6 2139.0 7 41596.0 8 282614.0 9 428527.0 10 331829.0 11 193854.0 12 106020.0 13 63078.0 14 40483.0 15 28354.0 16 22145.0 17 18755.0 18 16677.0 19 15766.0 20 15384.0 21 15382.0 22 16007.0 23 17773.0 24 20349.0 25 24029.0 26 29257.0 27 34651.0 28 41109.0 29 49251.0 30 61583.0 31 79394.0 32 108373.0 33 155370.0 34 235867.0 35 399198.0 36 757951.0 37 1127898.0 38 353219.0 39 679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.884336094829685 19.22815302262224 15.192183409290902 12.695327473257167 2 33.283777985802324 33.80306576175989 21.437144083001794 11.476012169435993 3 28.743118557726127 24.959465877638813 32.896803516934646 13.400612047700413 4 22.33656543688957 19.288248416130198 31.817833577630434 26.557352569349803 5 39.60751843991514 16.491524676948128 22.962056489676495 20.938900393460237 6 46.18531437667445 15.623557434775348 23.774644056012598 14.416484132537608 7 44.83552509832262 20.292971222979542 19.641382964424643 15.230120714273193 8 25.4405705313143 41.15552381781629 18.785560081819476 14.61834556904994 9 39.09260021663831 27.16354945620358 18.8545239897126 14.889326337445514 10-11 32.642048519199754 33.160874798998506 20.97526761579224 13.221809066009493 12-13 38.999090950256424 20.986360358614526 20.65521695711301 19.35933173401604 14-15 31.943712035447785 20.27546044382768 23.583186648650624 24.197640872073915 16-17 38.72943442508412 19.97770049229328 24.313523288135443 16.97934179448715 18-19 39.82389117032425 20.021632661287615 25.306815651422255 14.847660516965885 20-21 35.448649632886514 26.129337092507924 23.41975183021202 15.002261444393541 22-23 44.51587195993678 20.367929245754397 20.38197427986084 14.734224514447977 24-25 33.422461331224326 31.060424323073327 20.553390298188752 14.96372404751359 26-27 24.26296502051597 40.0409832071187 20.64472773700574 15.05132403535959 28-29 30.424402733301946 34.13339813726377 20.410314894094682 15.031884235339604 30-31 40.62739663577808 22.773081269794268 21.636624198634248 14.962897895793411 32-33 30.754528422297156 21.486640231011318 32.61469952654465 15.144131820146873 34-35 20.226668151078666 29.09479391928383 27.508967699110336 23.169570230527167 36-37 28.8091060697668 33.4903507970016 21.108183409640354 16.592359723591247 38-39 34.81009984258696 24.590633879974433 20.675778403258207 19.923487874180406 40-41 27.289032024640374 22.270915308376427 20.621383755315144 29.818668911668055 42-43 37.30478956409423 21.369475988554047 23.418967828830286 17.906766618521434 44-45 31.311348300826776 26.020377648687965 25.714778288259165 16.953495762226098 46-47 32.82955352094506 29.304275629153885 21.58160356635199 16.284567283549066 48-49 22.97919346119242 30.126915788261606 27.15101085702799 19.742879893517987 50-51 18.680889509287336 24.515744725035518 32.84932690736903 23.95403885830811 52-53 34.34927394411541 22.45133030642141 22.887755154809927 20.311640594653248 54-55 38.88448597912003 21.731346836710152 22.91071741303386 16.473449771135957 56-57 41.253372516368735 21.285333789718514 21.615103955612508 15.846189738300243 58-59 37.41956765887897 21.671302849039847 21.90303228864994 19.006097203431246 60-61 28.19897419009848 21.607937804495247 20.97497591155194 29.21811209385433 62-63 33.894619886588565 21.495875739568216 21.566976110957935 23.042528262885284 64-65 28.016463403690494 24.318528000973853 27.423217472526527 20.241791122809126 66-67 21.13283892044482 26.16007344879086 25.5348202271383 27.172267403626023 68-69 18.474514223241492 23.94995521264265 38.08662634780292 19.48890421631294 70-71 17.363043223843334 32.08486775376453 32.58176640399469 17.970322618397443 72-73 16.53041353608569 43.410756475014416 22.508517202242178 17.550312786657713 74-75 16.128080065741095 45.68799328858864 21.14003888725767 17.043887758412595 76-77 16.10833076536006 45.8898027406451 20.81522099522942 17.186645498765422 78-79 16.15680848965968 45.07869087578288 21.14858552647299 17.615915108084444 80-81 16.390809476732255 43.753555179110805 21.444099124440825 18.411536219716115 82-83 16.75029415876447 42.563048898400915 21.55218660860334 19.13447033423128 84-85 16.995858503957717 41.78335028066498 21.558156921105553 19.66263429427175 86-87 17.38962606349023 40.71404852839954 21.785658821564642 20.11066658654559 88-89 18.057088671870616 39.81235144082583 21.812596305854957 20.317963581448595 90-91 18.458498679184178 39.16346705374179 21.857024125861845 20.521010141212184 92-93 18.797212332177413 38.455878587908046 21.841897237056614 20.905011842857927 94-95 19.09989956578636 38.01694410423468 21.9381657619005 20.944990568078463 96-97 19.281997287485915 37.84763841157173 21.930931946798516 20.939432354143843 98-99 19.65381553379779 37.25933330478314 21.997227379196328 21.08962378222274 100-101 20.027147107118108 37.757677047368716 21.589422734736434 20.625753110776742 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1264.0 1 1031.5 2 579.5 3 351.5 4 376.0 5 453.5 6 600.0 7 788.0 8 977.5 9 1197.0 10 1417.5 11 1529.0 12 1578.5 13 1588.0 14 1560.0 15 1579.0 16 1562.0 17 1541.0 18 1632.5 19 1884.0 20 2286.0 21 2914.5 22 3866.0 23 5156.0 24 6871.5 25 9309.0 26 12180.5 27 15603.5 28 19945.5 29 24809.5 30 30500.5 31 37471.5 32 46517.0 33 58323.0 34 73358.0 35 90757.0 36 112189.0 37 136110.0 38 156530.0 39 170489.5 40 180658.0 41 188531.5 42 195114.5 43 201481.5 44 205632.0 45 210703.0 46 217540.5 47 223872.5 48 226002.5 49 222193.0 50 214645.5 51 208634.5 52 202752.5 53 192281.0 54 177699.0 55 160017.0 56 141190.5 57 120196.0 58 99967.5 59 84023.0 60 70800.0 61 59408.5 62 49941.5 63 41901.5 64 34917.5 65 29218.0 66 24651.0 67 20852.0 68 17687.5 69 15183.5 70 12860.5 71 10613.5 72 8713.0 73 7185.0 74 5854.0 75 4627.0 76 3657.0 77 2908.5 78 2241.0 79 1677.0 80 1278.5 81 984.0 82 741.0 83 567.0 84 409.0 85 298.5 86 232.0 87 175.5 88 138.0 89 110.0 90 77.0 91 53.5 92 34.5 93 25.0 94 18.5 95 12.0 96 6.5 97 2.0 98 2.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29020196851075236 2 0.2654414386237424 3 0.23797728449926644 4 0.22492597612991555 5 0.2311695975703949 6 0.18147348298963267 7 0.14854372878797692 8 0.1459957088231707 9 0.12489187934366895 10-11 0.11112090121326595 12-13 0.1315731378010043 14-15 0.13487972859502764 16-17 0.1284610523478059 18-19 0.11585710626235231 20-21 0.12165336541893436 22-23 0.1509361444801232 24-25 0.5555558797311235 26-27 0.2158425767133927 28-29 0.19199622192825982 30-31 0.2902116937777936 32-33 0.3204767248101482 34-35 0.3283930921817217 36-37 0.31987375825359105 38-39 0.3273622138753497 40-41 0.330153365516187 42-43 0.34064692865369045 44-45 0.3221689212753248 46-47 0.34194038917017605 48-49 0.3379919307514306 50-51 0.34345753082861025 52-53 0.32837364164763916 54-55 0.3205350764123957 56-57 0.3291127619427738 58-59 0.32133254830977775 60-61 0.2984878960298931 62-63 0.2857575214729034 64-65 0.29586207392875696 66-67 0.31205464355243 68-69 0.29873102770592425 70-71 0.2810018658897345 72-73 0.2775591213571338 74-75 0.26959412765035407 76-77 0.2720546202117891 78-79 0.2809726900886108 80-81 0.29026032011299985 82-83 0.3119573908820175 84-85 0.3027086619257935 86-87 0.30480931960670243 88-89 0.31071255670073816 90-91 0.30395349610707284 92-93 0.2866911471088629 94-95 0.28218834846876645 96-97 0.29905196151828534 98-99 0.3067640982819927 100-101 0.30807700933256077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5141247.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.52553695885275 #Duplication Level Percentage of deduplicated Percentage of total 1 94.45499468841763 76.06039165730412 2 2.9814122974285064 4.801596522923146 3 0.8869235267232483 2.1425997969248685 4 0.415590890912186 1.3386271858364711 5 0.2988441958251742 1.2032294667929346 6 0.13617847453930443 0.6579506870708967 7 0.1388567563722013 0.7827060407065276 8 0.11580474156454915 0.7460191197493196 9 0.07211279715877643 0.5226229541533771 >10 0.44220090738658513 6.529340980856102 >50 0.03857861525077881 2.1424933558439068 >100 0.01806881921468932 2.437769299373835 >500 3.59737320784372E-4 0.1728146327775315 >1k 4.9436207566072503E-5 0.11555371258819762 >5k 0.0 0.0 >10k+ 2.4115678027210293E-5 0.3462845870988147 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT 17832 0.34684192375896356 Illumina Single End PCR Primer 1 (96% over 32bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007002192269696438 0.0 0.0 2 0.007391202951346239 0.0 0.0 3 0.008266476985058294 0.0 0.0 4 0.008713839268955567 0.0 1.9450534082490105E-5 5 0.009978123984317423 0.0 1.9450534082490105E-5 6 0.010717244279452047 0.0 3.890106816498021E-5 7 0.012195484869721294 0.0 3.890106816498021E-5 8 0.014101637209805326 0.0 3.890106816498021E-5 9 0.015268669254754732 0.0 3.890106816498021E-5 10-11 0.023875530586256605 0.0 3.890106816498021E-5 12-13 0.031120854531984165 0.0 3.890106816498021E-5 14-15 0.037315849637257266 0.0 3.890106816498021E-5 16-17 0.040097276011053354 0.0 3.890106816498021E-5 18-19 0.04354002054365409 0.0 3.890106816498021E-5 20-21 0.04714809461595601 0.0 3.890106816498021E-5 22-23 0.05148556371635131 0.0 3.890106816498021E-5 24-25 0.055823032816746596 0.0 3.890106816498021E-5 26-27 0.06015077665010064 0.0 3.890106816498021E-5 28-29 0.06528571764787802 0.0 3.890106816498021E-5 30-31 0.11162661509941071 0.0 3.890106816498021E-5 32-33 0.11970831201068535 0.0 3.890106816498021E-5 34-35 0.12609781245678334 0.0 3.890106816498021E-5 36-37 0.13309027945943855 0.0 3.890106816498021E-5 38-39 0.14030642760404236 0.0 3.890106816498021E-5 40-41 0.14832977291306954 0.0 3.890106816498021E-5 42-43 0.15776328194307723 0.0 3.890106816498021E-5 44-45 0.16799426287046704 0.0 3.890106816498021E-5 46-47 0.17890601249074398 9.725267041245053E-6 3.890106816498021E-5 48-49 0.1902262233267532 1.9450534082490105E-5 3.890106816498021E-5 50-51 0.2029760484178255 1.9450534082490105E-5 3.890106816498021E-5 52-53 0.21698043295721836 1.9450534082490105E-5 3.890106816498021E-5 54-55 0.2316266851213334 1.9450534082490105E-5 3.890106816498021E-5 56-57 0.24749832093264532 1.9450534082490105E-5 3.890106816498021E-5 58-59 0.2661124820495884 1.9450534082490105E-5 3.890106816498021E-5 60-61 0.28459048942795395 1.9450534082490105E-5 3.890106816498021E-5 62-63 0.3040604740445265 1.9450534082490105E-5 3.890106816498021E-5 64-65 0.3256116658079256 1.9450534082490105E-5 3.890106816498021E-5 66-67 0.3495844490645946 1.9450534082490105E-5 3.890106816498021E-5 68-69 0.3754925604624715 1.9450534082490105E-5 3.890106816498021E-5 70-71 0.40329709893339105 1.9450534082490105E-5 3.890106816498021E-5 72-73 0.4325118011252912 1.9450534082490105E-5 3.890106816498021E-5 74-75 0.4642258969467913 1.9450534082490105E-5 3.890106816498021E-5 76-77 0.49913960562486104 1.9450534082490105E-5 3.890106816498021E-5 78-79 0.5366694111370256 1.9450534082490105E-5 3.890106816498021E-5 80-81 0.5761831711256044 1.9450534082490105E-5 3.890106816498021E-5 82-83 0.6207637952426717 1.9450534082490105E-5 3.890106816498021E-5 84-85 0.6715880408002183 1.9450534082490105E-5 5.835160224747031E-5 86-87 0.7234820657323019 1.9450534082490105E-5 6.807686928871537E-5 88-89 0.7830104252917629 1.9450534082490105E-5 7.780213632996042E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 14905 0.0 88.53714 9 ATCGGGA 23260 0.0 86.50489 2 TCGGGAG 21490 0.0 84.99409 3 GAGAGCG 18350 0.0 82.85813 7 TCCGGAG 4060 0.0 82.56228 3 GATCGGG 25575 0.0 80.547195 1 GGATCGG 7615 0.0 80.21354 1 CGGAGAG 11020 0.0 79.91947 5 AGAGCGG 17150 0.0 79.805244 8 CGGGAGA 23510 0.0 77.08321 4 CCGGAGA 5320 0.0 76.83901 4 TCCGGAA 2815 0.0 76.405174 3 ATTGGGA 10485 0.0 75.90974 2 TCGGAGA 6340 0.0 75.634926 4 CGGAAAG 9705 0.0 75.63177 5 GGAGAGC 20450 0.0 75.60068 6 GAGCGTC 22030 0.0 75.24908 9 ATCCGGA 5005 0.0 74.67282 2 ACCGGAA 2655 0.0 73.677635 3 GAGGGGC 23180 0.0 71.98634 9 >>END_MODULE