##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_30521-1.342000000356dc.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3162340 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76896823238488 33.0 33.0 33.0 27.0 33.0 2 31.620055402012433 33.0 33.0 33.0 27.0 33.0 3 31.562628623108203 33.0 33.0 33.0 27.0 33.0 4 35.22238532226136 37.0 37.0 37.0 33.0 37.0 5 35.2551322754669 37.0 37.0 37.0 33.0 37.0 6 35.37061827633967 37.0 37.0 37.0 33.0 37.0 7 35.227338300119534 37.0 37.0 37.0 33.0 37.0 8 35.19269401772105 37.0 37.0 37.0 33.0 37.0 9 35.01446460532391 37.0 37.0 37.0 33.0 37.0 10-11 34.98218075855221 37.0 37.0 37.0 33.0 37.0 12-13 34.98323077214974 37.0 37.0 37.0 33.0 37.0 14-15 36.69280975480183 40.0 37.0 40.0 33.0 40.0 16-17 36.6490235079087 40.0 37.0 40.0 33.0 40.0 18-19 36.6141066425495 40.0 37.0 40.0 33.0 40.0 20-21 36.58864385233719 40.0 37.0 40.0 33.0 40.0 22-23 36.738545665551456 40.0 37.0 40.0 33.0 40.0 24-25 36.397686207049205 40.0 37.0 40.0 33.0 40.0 26-27 36.304730199788764 40.0 37.0 40.0 33.0 40.0 28-29 36.22036846132927 40.0 37.0 40.0 33.0 40.0 30-31 36.21481576933536 40.0 37.0 40.0 33.0 40.0 32-33 36.110838967346965 40.0 37.0 40.0 33.0 40.0 34-35 36.04271662756061 40.0 37.0 40.0 30.0 40.0 36-37 35.939931664526895 37.0 37.0 40.0 27.0 40.0 38-39 35.832476425684774 37.0 37.0 40.0 27.0 40.0 40-41 35.76283527387947 37.0 37.0 40.0 27.0 40.0 42-43 35.642308069341055 37.0 37.0 40.0 27.0 40.0 44-45 35.49766454587426 37.0 37.0 40.0 27.0 40.0 46-47 35.34908311566751 37.0 37.0 40.0 27.0 40.0 48-49 35.219283821473965 37.0 37.0 40.0 27.0 40.0 50-51 34.0581242054934 37.0 35.0 38.5 24.5 38.5 52-53 34.4853913557682 37.0 33.0 38.5 27.0 40.0 54-55 35.16580664950638 37.0 37.0 40.0 27.0 40.0 56-57 35.23660469778709 37.0 37.0 40.0 27.0 40.0 58-59 34.941758634428936 37.0 35.0 40.0 27.0 40.0 60-61 34.672863132996454 37.0 33.0 40.0 27.0 40.0 62-63 34.378043948468544 37.0 33.0 38.5 27.0 40.0 64-65 34.14979429789334 37.0 33.0 37.0 27.0 40.0 66-67 33.88266663293637 37.0 33.0 37.0 27.0 40.0 68-69 33.74113710100748 37.0 33.0 37.0 27.0 40.0 70-71 33.43258520589184 37.0 33.0 37.0 27.0 38.5 72-73 33.300320490522836 37.0 33.0 37.0 27.0 37.0 74-75 33.16639750943921 37.0 33.0 37.0 27.0 37.0 76-77 32.93759336440737 37.0 33.0 37.0 24.5 37.0 78-79 32.71609962875592 37.0 33.0 37.0 22.0 37.0 80-81 32.44978259769664 37.0 33.0 37.0 22.0 37.0 82-83 32.23670098724362 35.0 33.0 37.0 22.0 37.0 84-85 32.00480735784261 33.0 33.0 37.0 22.0 37.0 86-87 31.880532453815846 33.0 33.0 37.0 22.0 37.0 88-89 31.787109703573936 33.0 33.0 37.0 22.0 37.0 90-91 31.658405010213954 33.0 33.0 37.0 22.0 37.0 92-93 31.50227141926548 33.0 33.0 37.0 15.0 37.0 94-95 31.354253495829038 33.0 33.0 37.0 15.0 37.0 96-97 31.19188449059873 33.0 33.0 37.0 15.0 37.0 98-99 31.033028074147627 33.0 33.0 37.0 15.0 37.0 100-101 29.629590587982317 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.3554877846038629 2306 2 0.45043219669685897 2306 3 0.38990773264124456 2306 4 0.5918103759478228 2306 5 0.4841048586059955 2306 6 0.3821014002280023 2306 7 0.5457883755245803 2306 8 0.5292617828476835 2306 9 0.6213139946675668 2306 10-11 0.5374635287477219 2306 12-13 0.642709906248804 2306 14-15 0.7919000593150258 2306 16-17 0.8516886988325894 2306 18-19 0.7635165083957176 2306 20-21 0.7345399607896397 2306 22-23 0.6417218689684177 2306 24-25 0.7285360174305353 2306 26-27 0.6506268752448321 2306 28-29 0.6751370850483482 2306 30-31 0.5338519888246509 2306 32-33 0.6451878436995813 2306 34-35 0.6853232883882967 2306 36-37 0.6779178943691875 2306 38-39 0.7301995505565202 2306 40-41 0.6114112610190716 2306 42-43 0.6836653784286355 2306 44-45 0.7454604716090572 2306 46-47 0.7017295882736079 2306 48-49 0.5443043564787118 2306 50-51 0.5997948865342195 2306 52-53 0.612896124336217 2306 54-55 0.6912876357370621 2306 56-57 0.5482604221959804 2306 58-59 0.5663061061571142 2306 60-61 0.5643180154753935 2306 62-63 0.6156863775359014 2306 64-65 0.6039642968871703 2306 66-67 0.6295285585191834 2306 68-69 0.4269571746149552 2306 70-71 0.6347063589078346 2306 72-73 0.6015441982961818 2306 74-75 0.5864320715161213 2306 76-77 0.5932331963682884 2306 78-79 0.7067712696709947 2306 80-81 0.6272982483600629 2306 82-83 0.7511846595955589 2306 84-85 0.7657189791740748 2306 86-87 0.6859165344616152 2306 88-89 0.7741798352922586 2306 90-91 0.5442767949025189 2306 92-93 0.4101348411011898 2306 94-95 0.40391758773016306 2306 96-97 0.3994379202589471 2306 98-99 0.40649827406213745 2306 100-101 0.2204169108712044 2307 1 0.10766518356463806 2307 2 0.11385994207157779 2307 3 0.06627511224998273 2307 4 0.19091422874436859 2307 5 0.1529390186746653 2307 6 0.09386613978364267 2307 7 0.1606570513865222 2307 8 0.026791510467830904 2307 9 0.29134035748444376 2307 10-11 0.22331061228743465 2307 12-13 0.2513255367828009 2307 14-15 0.389065677676129 2307 16-17 0.40208531965083694 2307 18-19 0.40600199436691753 2307 20-21 0.3403990118157836 2307 22-23 0.3378389684995824 2307 24-25 0.30167717618897427 2307 26-27 0.19719770549004778 2307 28-29 0.19238698428103618 2307 30-31 0.14340390512094103 2307 32-33 0.11068602483694434 2307 34-35 0.01876241196690387 2307 36-37 0.11049580678024995 2307 38-39 0.09370252205579277 2307 40-41 0.104661113782619 2307 42-43 -0.02006659836508362 2307 44-45 0.1024809143252412 2307 46-47 0.06876126776587199 2307 48-49 -0.0026665966066516944 2307 50-51 -0.11685045932916438 2307 52-53 -0.012936573975032672 2307 54-55 0.04032313175974167 2307 56-57 -0.1038260757248608 2307 58-59 0.07121323017886283 2307 60-61 0.12705017174773303 2307 62-63 0.07215717117825449 2307 64-65 0.1464831362497634 2307 66-67 0.14682989369871535 2307 68-69 0.05585346863394136 2307 70-71 0.08003483004291922 2307 72-73 0.03524460975008026 2307 74-75 0.07904533077719833 2307 76-77 0.13803247495177118 2307 78-79 0.11562912899949396 2307 80-81 0.08715194466000753 2307 82-83 0.1678457140476226 2307 84-85 0.1486940343393428 2307 86-87 0.18299445074510956 2307 88-89 0.03556990901318713 2307 90-91 0.15882591126494106 2307 92-93 0.06342036061099066 2307 94-95 0.3484640809434829 2307 96-97 0.11030551924915599 2307 98-99 -0.03342369819579005 2307 100-101 0.0775313020598496 2201 1 0.14794185282358896 2201 2 0.10880467020454176 2201 3 0.04576742518223753 2201 4 0.12605229818134944 2201 5 0.16691849724870877 2201 6 0.046815559587194855 2201 7 0.21880258263738028 2201 8 0.005747687248344846 2201 9 0.1334441071785193 2201 10-11 0.02624159641997892 2201 12-13 0.05434965779748069 2201 14-15 0.2968532949119762 2201 16-17 0.21695475867605296 2201 18-19 0.22693379772061206 2201 20-21 0.08184208073834753 2201 22-23 0.09419128727246573 2201 24-25 0.24418344335858677 2201 26-27 0.13463927527624975 2201 28-29 0.06619705277738319 2201 30-31 0.09668162045245055 2201 32-33 0.18303357270099951 2201 34-35 0.12840560984331972 2201 36-37 0.009703488288536732 2201 38-39 -0.02461135211260057 2201 40-41 0.12779851991885494 2201 42-43 0.15323653710482432 2201 44-45 0.0815415705584499 2201 46-47 0.1517757843391223 2201 48-49 0.18053444933810425 2201 50-51 0.046940572819195836 2201 52-53 0.14916633221519504 2201 54-55 0.2594339573007787 2201 56-57 0.25318628480145833 2201 58-59 0.28976507467254464 2201 60-61 0.2839580208704575 2201 62-63 0.34891221830263675 2201 64-65 0.27167316424513643 2201 66-67 0.39525758976382974 2201 68-69 0.3408531267592849 2201 70-71 0.40642480615003507 2201 72-73 0.3211880975489976 2201 74-75 0.29761602630362205 2201 76-77 0.3190092587866644 2201 78-79 0.3239748965592284 2201 80-81 0.4313820269463662 2201 82-83 0.35572405267735263 2201 84-85 0.35783058674744694 2201 86-87 0.26064504972868363 2201 88-89 0.28248584810454247 2201 90-91 0.2884833145128134 2201 92-93 0.39191829754118146 2201 94-95 0.34472614480566577 2201 96-97 0.22168157434784774 2201 98-99 0.27758846806904103 2201 100-101 0.10285670603108699 1101 1 0.10717015595118795 1101 2 0.1096067581718927 1101 3 0.07814349140919674 1101 4 0.19473840918500684 1101 5 0.09692136228033377 1101 6 0.10499474132448938 1101 7 0.18862304065559954 1101 8 0.14654857843033398 1101 9 0.23438116593946745 1101 10-11 0.16194300330766964 1101 12-13 0.16042624480740386 1101 14-15 0.2442441517135876 1101 16-17 0.16298759653218298 1101 18-19 0.21133146411003878 1101 20-21 0.26140280006679717 1101 22-23 0.24829931347118617 1101 24-25 0.15112567489182283 1101 26-27 0.09999233904677851 1101 28-29 0.23599747370734292 1101 30-31 0.20743428360210459 1101 32-33 0.13748092986043758 1101 34-35 0.18047030636756745 1101 36-37 0.12101268685336208 1101 38-39 0.09548379232367665 1101 40-41 0.09708737555027369 1101 42-43 0.09640645886649679 1101 44-45 0.2533299849508168 1101 46-47 0.20511312307531426 1101 48-49 0.158753862405554 1101 50-51 0.1699302151858788 1101 52-53 0.1060891613190833 1101 54-55 0.2664268406589372 1101 56-57 0.18389014570356466 1101 58-59 0.30997714745643634 1101 60-61 0.36029585161841027 1101 62-63 0.31973362407815387 1101 64-65 0.2845695842853715 1101 66-67 0.32860314279955816 1101 68-69 0.2809889256316467 1101 70-71 0.26559203091358086 1101 72-73 0.06058974717858945 1101 74-75 0.294455122742761 1101 76-77 0.30570711037560727 1101 78-79 0.32934501754335344 1101 80-81 0.46608729135130744 1101 82-83 0.327904232944654 1101 84-85 0.3059426880607745 1101 86-87 0.3917077044645687 1101 88-89 0.4201976126255431 1101 90-91 0.32107274701895605 1101 92-93 0.3826466864988447 1101 94-95 0.4176271464787469 1101 96-97 0.25909018346565915 1101 98-99 0.34708240919203703 1101 100-101 0.10833003464175661 2304 1 -0.038206265633913716 2304 2 -0.10810192521479678 2304 3 -0.13726755229514964 2304 4 -0.1285978609117393 2304 5 -0.1332944511404932 2304 6 -0.043430737106326944 2304 7 -0.01946009664785464 2304 8 -0.05134496629101193 2304 9 0.036779455173451936 2304 10-11 -0.0769270963138382 2304 12-13 0.06086692513426328 2304 14-15 0.11701656876199706 2304 16-17 0.06894355171355926 2304 18-19 0.15331564759400607 2304 20-21 0.1770357039030941 2304 22-23 0.0170238056358869 2304 24-25 -0.03657316462602722 2304 26-27 -0.033287896719684795 2304 28-29 -0.04886825503741221 2304 30-31 -0.05046913815102272 2304 32-33 -0.03314009611278834 2304 34-35 -0.00765564430365373 2304 36-37 0.06518479592851634 2304 38-39 -0.03855794766995757 2304 40-41 -0.018006575085571797 2304 42-43 -0.008378236211235901 2304 44-45 -0.11524289726322934 2304 46-47 -0.10001972770024992 2304 48-49 -0.08231563166049227 2304 50-51 0.013718352577768655 2304 52-53 0.04032026175418224 2304 54-55 0.10061057334787193 2304 56-57 0.07817453899846072 2304 58-59 0.11657054979818327 2304 60-61 -0.014889999077567495 2304 62-63 0.017547301362860424 2304 64-65 0.21846712072039765 2304 66-67 0.23893978542677274 2304 68-69 0.07044993374108799 2304 70-71 0.05134486855662601 2304 72-73 0.18973387225155136 2304 74-75 0.3224548773369378 2304 76-77 0.19132174142674074 2304 78-79 0.22628590126962678 2304 80-81 0.1784946009075412 2304 82-83 0.13997090373826637 2304 84-85 0.23769057447545805 2304 86-87 0.4289553620549107 2304 88-89 0.07584611962955279 2304 90-91 0.21392823172862308 2304 92-93 0.05827365969433629 2304 94-95 0.05152904936878855 2304 96-97 0.14607195710863152 2304 98-99 0.03957177493957431 2304 100-101 0.13248330163444777 2202 1 0.227137113302323 2202 2 0.2127926486201801 2202 3 0.2233352750309443 2202 4 0.30981922741637646 2202 5 0.27917754500277425 2202 6 0.3125225891239225 2202 7 0.3740791654682454 2202 8 0.3538890710042537 2202 9 0.33472479245534004 2202 10-11 0.2707697763693915 2202 12-13 0.3060885455295761 2202 14-15 0.3702565086423206 2202 16-17 0.37736881331920813 2202 18-19 0.41927873213021627 2202 20-21 0.411533948724653 2202 22-23 0.23524512523027852 2202 24-25 0.36398782402658725 2202 26-27 0.38846828944874545 2202 28-29 0.30863275269631174 2202 30-31 0.4038836891750677 2202 32-33 0.2678343925256499 2202 34-35 0.2846291665665035 2202 36-37 0.438151857721337 2202 38-39 0.2698018618835505 2202 40-41 0.22049272709680423 2202 42-43 0.3975480483200968 2202 44-45 0.2632608764210431 2202 46-47 0.4221002807262195 2202 48-49 0.42829203177468145 2202 50-51 0.20402886622824923 2202 52-53 0.3343974713749489 2202 54-55 0.5544226932955354 2202 56-57 0.46784934484514196 2202 58-59 0.4700061875163897 2202 60-61 0.3501179918963757 2202 62-63 0.5482388700244698 2202 64-65 0.5259225019172007 2202 66-67 0.5946933373083425 2202 68-69 0.47532632684433906 2202 70-71 0.47208623141773387 2202 72-73 0.3826267668679222 2202 74-75 0.2974975161206075 2202 76-77 0.3892611538420141 2202 78-79 0.48398759486995857 2202 80-81 0.43569103504935924 2202 82-83 0.4803347411882939 2202 84-85 0.5292019876928791 2202 86-87 0.5784806384474059 2202 88-89 0.3989061815722579 2202 90-91 0.5621818899539583 2202 92-93 0.4211300885290967 2202 94-95 0.4437578778454174 2202 96-97 0.3466021060709181 2202 98-99 0.4446433848017932 2202 100-101 0.46162344144104495 1102 1 0.04031947495707655 1102 2 -0.012477387343622581 1102 3 0.07046320761347147 1102 4 0.01929195848997267 1102 5 0.04527470592424976 1102 6 0.14313301843829862 1102 7 0.12632643034611846 1102 8 0.10311933016688357 1102 9 0.11952393621029955 1102 10-11 0.10517824413344101 1102 12-13 0.080739170686833 1102 14-15 0.09162167653332176 1102 16-17 0.09318286095943051 1102 18-19 0.15930112910554328 1102 20-21 0.15348779217899988 1102 22-23 0.15395121098342912 1102 24-25 0.1657084963094846 1102 26-27 0.24045640824469672 1102 28-29 0.18805474235931996 1102 30-31 0.2608003958856031 1102 32-33 0.13761121024912626 1102 34-35 0.18718848981887248 1102 36-37 0.2652759877131814 1102 38-39 0.12137306728588726 1102 40-41 0.08775919647649033 1102 42-43 0.17059297753704783 1102 44-45 0.20401282453932623 1102 46-47 0.15215772633120395 1102 48-49 0.16237370849014354 1102 50-51 0.20064567197641026 1102 52-53 0.2907027957586408 1102 54-55 0.4345402283465276 1102 56-57 0.3233108748297937 1102 58-59 0.3331115632634294 1102 60-61 0.3850875191454364 1102 62-63 0.5080977721541089 1102 64-65 0.4131481974980673 1102 66-67 0.5191912203436786 1102 68-69 0.31248032183476226 1102 70-71 0.3201317636574572 1102 72-73 0.327404305853932 1102 74-75 0.4134410599459386 1102 76-77 0.3704143273025835 1102 78-79 0.3489090150902783 1102 80-81 0.3990875525573543 1102 82-83 0.3622373418954581 1102 84-85 0.3512792308903059 1102 86-87 0.29040382942637066 1102 88-89 0.25487283181148257 1102 90-91 0.28084675732678477 1102 92-93 0.24367532469646136 1102 94-95 0.23306319797247355 1102 96-97 0.28702735773242694 1102 98-99 0.3233154979071102 1102 100-101 0.3357821064290256 2305 1 0.034735525303936754 2305 2 -0.04320230486738552 2305 3 -0.038317537482146946 2305 4 -0.04017760769809797 2305 5 -0.07774321719274013 2305 6 0.12726999938233519 2305 7 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1213 60-61 0.1338215860290788 1213 62-63 0.26448161158266004 1213 64-65 0.21668484470830407 1213 66-67 0.2015138645945811 1213 68-69 0.32428142082568456 1213 70-71 0.29488341472951163 1213 72-73 0.39520072729393974 1213 74-75 0.28406561475285486 1213 76-77 0.30939455781042113 1213 78-79 0.23976080812366973 1213 80-81 0.355760110647239 1213 82-83 0.35125969371601684 1213 84-85 0.32650243214343533 1213 86-87 0.25780560335919844 1213 88-89 0.22960509039797472 1213 90-91 0.2600798658463006 1213 92-93 0.2712128210056335 1213 94-95 0.4831074044986927 1213 96-97 0.40227022671929547 1213 98-99 0.2734919069692836 1213 100-101 0.33507388451559805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1423.0 3 332.0 4 339.0 5 359.0 6 996.0 7 3511.0 8 13690.0 9 28875.0 10 31694.0 11 20727.0 12 12513.0 13 8607.0 14 7032.0 15 6375.0 16 6651.0 17 7058.0 18 7638.0 19 8531.0 20 9951.0 21 11098.0 22 12586.0 23 14451.0 24 16329.0 25 19105.0 26 22889.0 27 27901.0 28 34140.0 29 42389.0 30 53234.0 31 69606.0 32 95990.0 33 139388.0 34 213406.0 35 365078.0 36 686137.0 37 946982.0 38 215276.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.534635909636826 25.0541119191472 18.98843931552426 16.422812855691717 2 34.03700657529695 22.844987485438782 28.57936425902193 14.538641680242339 3 39.72885189647407 19.798845027752236 22.846725439089465 17.625577636684238 4 33.51039200218809 18.565140211766817 29.105244158727817 18.81922362731728 5 35.701675649017645 16.876994405206087 28.113268903823553 19.308061041952715 6 31.965076569241784 17.3754205447599 31.554922670451308 19.10458021554701 7 36.5416880692805 17.737261829285362 24.597636138041633 21.123413963392505 8 31.790027573282003 25.458844810272574 22.49618163721389 20.254945979231525 9 31.20623041163003 23.78748009699716 24.077844684865298 20.92844480650751 10-11 25.27855003295495 28.48363237600424 28.596378817914577 17.641438773126236 12-13 26.169311206012846 25.989638208019507 28.11433592312168 19.72671466284597 14-15 25.69420784185081 26.030735890710076 28.10466756930996 20.170388698129145 16-17 25.71285096375025 26.1005864158344 28.609848404493626 19.576714215921722 18-19 25.259876247144803 26.111330082011197 28.553747330797492 20.075046340046505 20-21 24.435192966864854 26.989051409759814 28.17358809916434 20.40216752421099 22-23 25.180003414008013 26.5143009881938 27.723138518466207 20.58255707933198 24-25 24.288980967992398 26.652140860357477 28.289354952117467 20.76952321953266 26-27 23.11916646858411 27.44814198557959 28.769495285635056 20.663196260201254 28-29 23.390426405449478 27.938697335003564 27.825169187199617 20.84570707234734 30-31 23.664474781800042 27.959171439609086 27.82264382420686 20.553709954384015 32-33 23.58190416211251 27.614760690824696 28.211181562779096 20.59215358428369 34-35 23.004725522740923 27.47517220568922 28.106056303304122 21.414045968265732 36-37 21.45243771644439 27.963817787515005 28.77057604763803 21.813168448402575 38-39 21.401700268063426 28.08573968260415 28.845271227610546 21.66728882172188 40-41 21.96247870704463 27.43319560834828 29.328139732238263 21.276185952368827 42-43 22.91836377847459 27.135742816913115 28.75681449561705 21.189078908995246 44-45 22.148463687904155 27.583610132746323 28.78332102819943 21.484605151150088 46-47 22.63857553789489 28.02337144002286 28.443894247788105 20.894158774294144 48-49 21.663385244277293 28.317027765926504 28.70905315586944 21.310533833926762 50-51 21.557391602789263 27.80224765312131 27.975390599029847 22.66497014505958 52-53 22.98079670442555 27.07161284171111 27.689564781038833 22.258025672824505 54-55 23.89210511370757 26.924259359668973 27.922244385213872 21.26139114140958 56-57 24.470156822697177 27.055528488317027 26.97204751910646 21.50226716987933 58-59 23.790546335065326 27.55202726519636 26.982378959451523 21.67504744028679 60-61 22.598907422069285 27.300507145974805 27.082572043403673 23.01801338855223 62-63 23.15816231949053 27.09152959948595 27.500475343131136 22.24983273789239 64-65 22.779417593183076 27.68087350713667 28.10969083060796 21.430018069072293 66-67 22.527641869550838 27.641584031204125 27.791402527959114 22.039371571285926 68-69 22.719801861953318 26.92738052170156 29.060653924704432 21.29216369164069 70-71 22.182852470891053 28.084181743105475 28.5349209077641 21.198044878239376 72-73 22.092335521861337 29.484904674874677 27.135876161218498 21.28688364204549 74-75 22.452283631359144 29.087562192209997 26.9353708383938 21.524783338037057 76-77 22.145019177439277 28.697503841037342 26.72616773084674 22.43130925067664 78-79 21.873741342817148 28.77207070236163 27.14202441866292 22.212163536158307 80-81 21.345820175329393 28.891432254246176 27.609138417100255 22.153609153324176 82-83 20.833636696308204 28.84187992092418 27.777198810531623 22.547284572236 84-85 20.857870594256784 29.37007235838599 27.458675972070466 22.31338107528676 86-87 20.683016517501756 29.32623838438439 27.74359464830342 22.247150449810437 88-89 21.077312570000192 29.138844374455047 27.686060331699196 22.097782723845565 90-91 21.608163899740358 28.967394838560278 26.92656265117378 22.497878610525582 92-93 21.604709309913435 28.98959193793444 26.8129744209343 22.592724331217827 94-95 21.870767560130876 29.226445770933797 26.77413016992951 22.12865649900582 96-97 21.897102422253 29.937766874543925 26.567893645255484 21.59723705794759 98-99 22.1434846860982 29.460106388462705 26.750939111265986 21.645469814173104 100-101 22.260603642921666 29.575727390751606 26.43725052759473 21.726418438731994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 735.0 1 611.0 2 327.5 3 199.0 4 213.5 5 217.5 6 299.5 7 460.0 8 620.0 9 778.0 10 926.0 11 1037.5 12 1105.5 13 1059.0 14 937.5 15 788.0 16 652.0 17 584.0 18 536.0 19 566.0 20 663.5 21 772.0 22 977.0 23 1257.0 24 1591.0 25 2021.0 26 2664.5 27 3553.0 28 4489.0 29 5592.0 30 7183.0 31 9616.0 32 14408.0 33 22919.5 34 36902.5 35 56635.0 36 85521.5 37 118941.5 38 140124.5 39 144074.0 40 146017.0 41 149161.0 42 158080.0 43 173873.0 44 174277.5 45 166695.0 46 165281.0 47 162169.0 48 154053.0 49 139222.0 50 125367.5 51 121691.5 52 117563.0 53 106974.0 54 92994.5 55 77398.5 56 62839.0 57 48433.0 58 35109.0 59 25623.0 60 19511.0 61 15723.5 62 12447.5 63 9693.0 64 7464.0 65 5527.0 66 3994.0 67 2978.0 68 2415.5 69 1758.0 70 1213.0 71 999.0 72 788.0 73 557.5 74 446.0 75 374.0 76 290.5 77 227.5 78 171.5 79 131.0 80 110.5 81 93.5 82 81.0 83 67.5 84 55.0 85 45.5 86 27.0 87 23.5 88 23.5 89 20.0 90 17.5 91 11.5 92 8.0 93 6.0 94 4.5 95 5.5 96 7.0 97 5.0 98 3.5 99 2.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28943756838290635 2 0.266068797156536 3 0.2358696408355838 4 0.22378997830720287 5 0.2297033209585307 6 0.18087871639355668 7 0.14628408077562818 8 0.14460810665519835 9 0.12323153108141441 10-11 0.11010833749691684 12-13 0.12972988356723186 14-15 0.13306602073148366 16-17 0.1265202350158427 18-19 0.11463030540675576 20-21 0.1203381040621818 22-23 0.15055307145974184 24-25 0.5635542035328269 26-27 0.22420106629900644 28-29 0.19904564341595146 30-31 0.3003472112423079 32-33 0.33060961186969146 34-35 0.33933732615721274 36-37 0.3290126931323008 38-39 0.3371079643555089 40-41 0.3405547790560155 42-43 0.35268187481421986 44-45 0.33326587273980657 46-47 0.35238146435867107 48-49 0.34827058444063574 50-51 0.3537412169469443 52-53 0.33897367139523266 54-55 0.33010365741824094 56-57 0.3391001600080953 58-59 0.33149503215972986 60-61 0.3097706129005736 62-63 0.2958094322558612 64-65 0.30722502956671327 66-67 0.32230879665058154 68-69 0.3095966910578875 70-71 0.290481099439023 72-73 0.28709752904494773 74-75 0.27859116982993604 76-77 0.2812790528532669 78-79 0.29022812221329775 80-81 0.29960409064174 82-83 0.3217712200459154 84-85 0.31326486083090366 86-87 0.31484596849168656 88-89 0.31990551300619163 90-91 0.3138498706653933 92-93 0.2957145657962142 94-95 0.292141262482845 96-97 0.3085847821549865 98-99 0.3169962749103512 100-101 0.31819791673254616 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3162340.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.180776099851634 #Duplication Level Percentage of deduplicated Percentage of total 1 87.24004143841448 44.6501302780127 2 6.089764104890101 6.233577063065873 3 1.9297184341274058 2.962934613384932 4 1.0769206151409634 2.2047053152337655 5 0.6240876971747468 1.597064634788636 6 0.513122290104666 1.5757198225014015 7 0.33195637496884756 1.1892849431539287 8 0.29373651781883015 1.2026930360668502 9 0.1845557383309341 0.8501135329312511 >10 1.375545955276826 13.963222046519249 >50 0.19914602183468644 7.135005747111986 >100 0.13348034915732823 13.024299825824077 >500 0.006310722012905678 2.152632510634149 >1k 0.0016137407474335274 1.258616630771211 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4862 0.15374690893452317 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 4437 0.14030749381786903 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0015494855075671812 0.0 0.0 2 0.001707596273645465 0.0 0.0 3 0.001992195652586376 0.0 0.0 4 0.002213550725095973 0.0 0.0 5 0.002624638716899511 0.0 0.0 6 0.002751127329762138 0.0 0.0 7 0.0032570817812126464 0.0 0.0 8 0.0035733033133692137 0.0 0.0 9 0.0040160134583884085 0.0 0.0 10-11 0.005691987578818216 0.0 0.0 12-13 0.007842293997482878 0.0 0.0 14-15 0.010182333335441478 0.0 0.0 16-17 0.012016418221949569 0.0 0.0 18-19 0.014514568325986453 0.0 0.0 20-21 0.01728150673235642 0.0 0.0 22-23 0.020080067291942044 0.0 0.0 24-25 0.023558504145664287 0.0 0.0 26-27 0.02711599638242567 0.0 0.0 28-29 0.03201743013085247 0.0 0.0 30-31 0.06566340115231126 0.0 0.0 32-33 0.07347407299657849 0.0 0.0 34-35 0.07968782610345504 0.0 0.0 36-37 0.08743525364129094 0.0 0.0 38-39 0.09643175623114529 0.0 0.0 40-41 0.10613975726835191 0.0 0.0 42-43 0.11793482041779188 0.0 0.0 44-45 0.13124774692158336 0.0 0.0 46-47 0.14713787891245092 0.0 0.0 48-49 0.16415059734247425 0.0 0.0 50-51 0.1825705015905943 0.0 0.0 52-53 0.20359923347900605 0.0 0.0 54-55 0.22771112530594434 0.0 0.0 56-57 0.25346736910009676 0.0 0.0 58-59 0.28238582821581487 0.0 0.0 60-61 0.3154151672495683 0.0 0.0 62-63 0.348523561666361 0.0 0.0 64-65 0.3858060803076203 0.0 0.0 66-67 0.42566580443595564 0.0 0.0 68-69 0.4691304540308759 0.0 0.0 70-71 0.516105162632734 0.0 0.0 72-73 0.5696414680268409 0.0 0.0 74-75 0.6316841326359595 1.581107660782838E-5 0.0 76-77 0.7033399318226377 3.162215321565676E-5 0.0 78-79 0.7838973671395233 3.162215321565676E-5 0.0 80-81 0.8742260478000468 3.162215321565676E-5 0.0 82-83 0.9770771011339705 3.162215321565676E-5 0.0 84-85 1.0934782471208029 3.162215321565676E-5 0.0 86-87 1.2254533035663464 3.162215321565676E-5 0.0 88-89 1.3725911824787973 3.162215321565676E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 1135 0.0 61.406345 9 GGATCGG 800 0.0 54.01366 1 AGAGCGG 1380 0.0 52.22564 8 GATCGGG 2295 0.0 43.656803 1 TTGTGTT 26950 0.0 43.643486 1 ATCGGGA 2220 0.0 41.485634 2 TCGGGAA 785 0.0 40.50701 3 CGGGAGA 1900 0.0 37.967163 4 TCGGGAG 1925 0.0 37.474678 3 GAGAGCG 2085 0.0 36.38763 7 TCGGAAA 960 0.0 36.08858 4 GGCGCCG 965 0.0 34.70123 56-57 AAGGGGC 1740 0.0 34.60559 9 TCCGGAA 495 0.0 34.51615 3 CGCCGGA 590 0.0 32.602295 58-59 CGGAGAG 1150 0.0 31.776865 5 GAATCGG 420 0.0 31.656357 1 TGTGTTT 37675 0.0 31.338017 2 GAGGGGC 2395 0.0 30.486458 9 ACCGGAA 300 0.0 30.057816 3 >>END_MODULE