FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_30521-1.342000000356dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_30521-1.342000000356dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3162340
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA48620.15374690893452317No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC44370.14030749381786903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC11350.061.4063459
GGATCGG8000.054.013661
AGAGCGG13800.052.225648
GATCGGG22950.043.6568031
TTGTGTT269500.043.6434861
ATCGGGA22200.041.4856342
TCGGGAA7850.040.507013
CGGGAGA19000.037.9671634
TCGGGAG19250.037.4746783
GAGAGCG20850.036.387637
TCGGAAA9600.036.088584
GGCGCCG9650.034.7012356-57
AAGGGGC17400.034.605599
TCCGGAA4950.034.516153
CGCCGGA5900.032.60229558-59
CGGAGAG11500.031.7768655
GAATCGG4200.031.6563571
TGTGTTT376750.031.3380172
GAGGGGC23950.030.4864589
ACCGGAA3000.030.0578163
Produced by FastQC (version 0.11.2)