##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_30484-1.34200000035803.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8634207 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.570585347328365 33.0 33.0 33.0 27.0 33.0 2 31.320995431311758 33.0 33.0 33.0 27.0 33.0 3 31.228663385068252 33.0 33.0 33.0 27.0 33.0 4 34.81063217502198 37.0 37.0 37.0 33.0 37.0 5 34.85195965304052 37.0 37.0 37.0 33.0 37.0 6 35.100270239061906 37.0 37.0 37.0 33.0 37.0 7 34.87061695416846 37.0 37.0 37.0 33.0 37.0 8 34.86795834290283 37.0 37.0 37.0 33.0 37.0 9 34.597310210422336 37.0 37.0 37.0 33.0 37.0 10-11 34.58400754116736 37.0 37.0 37.0 33.0 37.0 12-13 34.56748291997169 37.0 37.0 37.0 33.0 37.0 14-15 36.21997926387449 40.0 37.0 40.0 33.0 40.0 16-17 36.17326153982641 40.0 37.0 40.0 33.0 40.0 18-19 36.151396764057196 40.0 37.0 40.0 27.0 40.0 20-21 36.11745369319962 40.0 37.0 40.0 27.0 40.0 22-23 36.44564660078221 40.0 37.0 40.0 27.0 40.0 24-25 36.011161882035026 40.0 37.0 40.0 27.0 40.0 26-27 35.86914281763224 40.0 37.0 40.0 27.0 40.0 28-29 35.74566749442074 40.0 37.0 40.0 27.0 40.0 30-31 35.73885899423074 40.0 37.0 40.0 27.0 40.0 32-33 35.56177811118033 40.0 37.0 40.0 27.0 40.0 34-35 35.6222107021525 40.0 37.0 40.0 27.0 40.0 36-37 35.43755321131402 37.0 37.0 40.0 27.0 40.0 38-39 35.33860243332132 37.0 37.0 40.0 27.0 40.0 40-41 35.508819918262326 37.0 37.0 40.0 27.0 40.0 42-43 35.333877679791556 37.0 37.0 40.0 27.0 40.0 44-45 35.042328322682096 37.0 37.0 40.0 22.0 40.0 46-47 34.872759652391935 37.0 37.0 40.0 22.0 40.0 48-49 34.728203180674264 37.0 37.0 40.0 22.0 40.0 50-51 33.66346550412794 37.0 33.0 38.5 22.0 38.5 52-53 34.08422481647707 37.0 33.0 38.5 22.0 40.0 54-55 34.77710755602686 37.0 37.0 40.0 22.0 40.0 56-57 34.942504447715926 37.0 37.0 40.0 22.0 40.0 58-59 34.61078255362652 37.0 33.0 40.0 22.0 40.0 60-61 34.32868675722044 37.0 33.0 40.0 22.0 40.0 62-63 34.01431752794437 37.0 33.0 40.0 22.0 40.0 64-65 33.78074205309184 37.0 33.0 38.5 22.0 40.0 66-67 33.52685104723572 37.0 33.0 37.0 22.0 40.0 68-69 33.40923978310921 37.0 33.0 37.0 22.0 40.0 70-71 33.084315560189836 37.0 33.0 37.0 22.0 40.0 72-73 33.02585547230915 37.0 33.0 37.0 22.0 37.0 74-75 32.93560584081433 37.0 33.0 37.0 22.0 37.0 76-77 32.69563151543622 37.0 33.0 37.0 22.0 37.0 78-79 32.380171566421794 37.0 33.0 37.0 22.0 37.0 80-81 32.00783928390876 37.0 33.0 37.0 22.0 37.0 82-83 31.724580265448814 33.0 33.0 37.0 15.0 37.0 84-85 31.437229614717367 33.0 33.0 37.0 15.0 37.0 86-87 31.27558882940842 33.0 33.0 37.0 15.0 37.0 88-89 31.14524501207812 33.0 33.0 37.0 15.0 37.0 90-91 30.99671006266123 33.0 33.0 37.0 15.0 37.0 92-93 30.830872713614582 33.0 33.0 37.0 15.0 37.0 94-95 30.68006940301524 33.0 27.0 37.0 15.0 37.0 96-97 30.528786604259082 33.0 27.0 37.0 15.0 37.0 98-99 30.375277312670406 33.0 27.0 37.0 15.0 37.0 100-101 29.050330505163934 33.0 27.0 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13708250026536817 2306 2 0.2663096875896862 2306 3 0.1953856365318849 2306 4 0.36596955434220035 2306 5 0.2667378316322271 2306 6 0.18095337457726401 2306 7 0.36755379865300597 2306 8 0.20510540618729323 2306 9 0.3676670436277334 2306 10-11 0.2903023264290283 2306 12-13 0.32429617148593337 2306 14-15 0.49720144213890904 2306 16-17 0.39696987211833346 2306 18-19 0.4137271012042518 2306 20-21 0.42121449448230663 2306 22-23 0.23673296605613103 2306 24-25 0.41593729826727355 2306 26-27 0.20336071424153346 2306 28-29 0.19950053588721772 2306 30-31 0.0886029194196638 2306 32-33 0.26556980338950353 2306 34-35 0.18686641253667347 2306 36-37 0.28772191601169084 2306 38-39 0.1747734594245074 2306 40-41 0.024853905746311966 2306 42-43 0.22620806507012503 2306 44-45 0.4040845617282187 2306 46-47 0.33417858109890375 2306 48-49 0.2988543671544761 2306 50-51 0.3060587885266983 2306 52-53 0.25466978566414866 2306 54-55 0.3150281766418388 2306 56-57 0.19193381407323074 2306 58-59 0.30533964265089963 2306 60-61 0.2007570829851275 2306 62-63 0.24747530923118433 2306 64-65 0.17193890597841488 2306 66-67 0.20942724989073724 2306 68-69 -0.01589604163083891 2306 70-71 0.05337695100511297 2306 72-73 -0.08559345953333519 2306 74-75 -0.031005700764715982 2306 76-77 -0.012528839391102053 2306 78-79 0.09159325884044733 2306 80-81 0.17470620735810627 2306 82-83 0.16864755804371256 2306 84-85 0.2810539850609004 2306 86-87 0.24455361181667357 2306 88-89 0.30641199615137893 2306 90-91 0.13726238209710928 2306 92-93 0.05309011503698713 2306 94-95 -0.03595244223406979 2306 96-97 -0.029775573504757347 2306 98-99 0.09881786329851394 2306 100-101 -0.044554031368349456 2307 1 0.14334986128631044 2307 2 0.15622363773536918 2307 3 0.12694956317607975 2307 4 0.22794527206812631 2307 5 0.24176227988827037 2307 6 0.12697109504546233 2307 7 0.3250940649685816 2307 8 0.08322121983218267 2307 9 0.27267347732237823 2307 10-11 0.2194069960188827 2307 12-13 0.2171705435893614 2307 14-15 0.4052113486869544 2307 16-17 0.3767760941264626 2307 18-19 0.43037193269469043 2307 20-21 0.2887211094153983 2307 22-23 0.22888452944609838 2307 24-25 0.35167534940529066 2307 26-27 0.08874915369512593 2307 28-29 0.12620501322364674 2307 30-31 0.09245027368447012 2307 32-33 0.24878727124916367 2307 34-35 0.036290285244305664 2307 36-37 0.2099746088364185 2307 38-39 0.048970532493797236 2307 40-41 -0.027390567508746244 2307 42-43 0.028423783627040677 2307 44-45 0.22092362674121802 2307 46-47 0.12433812828626145 2307 48-49 0.025405979321931227 2307 50-51 -0.13312718896208509 2307 52-53 -0.14188739241740222 2307 54-55 -0.035410225655155614 2307 56-57 -0.14599304847379102 2307 58-59 0.06202063310661288 2307 60-61 0.016071580936205976 2307 62-63 0.11614780138869918 2307 64-65 0.105983040301318 2307 66-67 0.068570697476801 2307 68-69 -0.06401224101607994 2307 70-71 0.005713753080165418 2307 72-73 -0.1426014339171502 2307 74-75 -0.14317373413163637 2307 76-77 -0.13671567384020733 2307 78-79 -0.03159619604622321 2307 80-81 0.036997672227933265 2307 82-83 0.15023220267784154 2307 84-85 0.1611871179607327 2307 86-87 0.1872197733961123 2307 88-89 0.0675860231551546 2307 90-91 0.11780962593088873 2307 92-93 0.15725556443045008 2307 94-95 0.1424937907544006 2307 96-97 0.09828484068408017 2307 98-99 0.012924631467370773 2307 100-101 0.004551716455061694 2201 1 0.21694286328622425 2201 2 0.24506874825247849 2201 3 0.23332836150377645 2201 4 0.3586442545695405 2201 5 0.32317791362704895 2201 6 0.23672938459197468 2201 7 0.4220083201311411 2201 8 0.18506368132436535 2201 9 0.37549645304710566 2201 10-11 0.33792811238812703 2201 12-13 0.377684238713762 2201 14-15 0.6068093045778014 2201 16-17 0.5057175597455554 2201 18-19 0.4754395943321512 2201 20-21 0.41871186172053143 2201 22-23 0.3786677689070075 2201 24-25 0.468084283762785 2201 26-27 0.3082417168145568 2201 28-29 0.29855618544419116 2201 30-31 0.2716221554513183 2201 32-33 0.32612344022253836 2201 34-35 0.16613561916065578 2201 36-37 0.28209819911545253 2201 38-39 0.16210288710760778 2201 40-41 0.07722814300380065 2201 42-43 0.22968622042194653 2201 44-45 0.338755078015744 2201 46-47 0.23450920087672245 2201 48-49 0.21872082220963307 2201 50-51 0.08083778202853154 2201 52-53 0.17372040960237456 2201 54-55 0.3097358766264904 2201 56-57 0.3003821624137686 2201 58-59 0.33394047448599196 2201 60-61 0.3246762731407884 2201 62-63 0.3310780711391317 2201 64-65 0.34639829984576664 2201 66-67 0.31740553958925943 2201 68-69 0.23379335903432974 2201 70-71 0.1928338467669377 2201 72-73 0.11399344760771157 2201 74-75 0.20169874920631514 2201 76-77 0.17234435776298085 2201 78-79 0.30468785129325227 2201 80-81 0.39720263045484216 2201 82-83 0.32785546821591893 2201 84-85 0.3422356694588551 2201 86-87 0.3089058316945348 2201 88-89 0.2935524794527673 2201 90-91 0.2572916319101388 2201 92-93 0.2729468500780712 2201 94-95 0.289204122737722 2201 96-97 0.22458798535766533 2201 98-99 0.2254298688160148 2201 100-101 0.08713041301831481 1101 1 0.19843806098517902 1101 2 0.21110540484555074 1101 3 0.17961494836930214 1101 4 0.28471177839359285 1101 5 0.25524724141703103 1101 6 0.23075507310462484 1101 7 0.38454188105086473 1101 8 0.28026918895271535 1101 9 0.32967000381014344 1101 10-11 0.28876483955648524 1101 12-13 0.3250318190953152 1101 14-15 0.48332166953957767 1101 16-17 0.30749597860921085 1101 18-19 0.3288755360802469 1101 20-21 0.34713546393908956 1101 22-23 0.3369164547901775 1101 24-25 0.3943634388777042 1101 26-27 0.31482106547788646 1101 28-29 0.3177802166534889 1101 30-31 0.3115238066820041 1101 32-33 0.2278017918680959 1101 34-35 0.20963922415412384 1101 36-37 0.2088958610253826 1101 38-39 0.17529475103618353 1101 40-41 0.056015871955523266 1101 42-43 0.20461872030762862 1101 44-45 0.332381889167209 1101 46-47 0.18371005802060125 1101 48-49 0.2208708421129728 1101 50-51 0.10815993558259862 1101 52-53 0.1814279631237099 1101 54-55 0.36688237080520736 1101 56-57 0.39692267643125234 1101 58-59 0.46063051043493886 1101 60-61 0.4300356582657727 1101 62-63 0.3731479805267597 1101 64-65 0.3428093155077647 1101 66-67 0.32327977710360756 1101 68-69 0.30309048173366904 1101 70-71 0.32085561297427034 1101 72-73 0.0028487533419792044 1101 74-75 0.12249947068460187 1101 76-77 0.22661373366142357 1101 78-79 0.33318488612474084 1101 80-81 0.4339354526712569 1101 82-83 0.2998206042405336 1101 84-85 0.28741451883985647 1101 86-87 0.33131176266652673 1101 88-89 0.3057278309393787 1101 90-91 0.23590065975110264 1101 92-93 0.310262688467958 1101 94-95 0.33850475663862056 1101 96-97 0.1855504220531934 1101 98-99 0.14327396673814263 1101 100-101 0.09120612230568526 2304 1 0.19016431947433077 2304 2 0.12343391682375326 2304 3 0.17155230096120277 2304 4 0.20653622951078887 2304 5 0.16335472572518484 2304 6 0.16891463156338204 2304 7 0.3270232846002372 2304 8 0.08828633426107757 2304 9 0.2984280155758796 2304 10-11 0.24763154556147526 2304 12-13 0.2720788420612408 2304 14-15 0.39311070542539284 2304 16-17 0.3675649140462056 2304 18-19 0.4105363651619456 2304 20-21 0.3830409902044991 2304 22-23 0.22739456065786356 2304 24-25 0.3097419524517804 2304 26-27 0.21367254024084303 2304 28-29 0.3033562463230979 2304 30-31 0.34937046497203994 2304 32-33 0.3610095245154312 2304 34-35 0.2550686401509097 2304 36-37 0.34514618042803136 2304 38-39 0.25504313101557585 2304 40-41 0.26610427211182497 2304 42-43 0.42120065945258744 2304 44-45 0.2756498150629838 2304 46-47 0.17806470966132082 2304 48-49 0.2500641983914278 2304 50-51 0.32753766629597436 2304 52-53 0.328497541851533 2304 54-55 0.4326285719809988 2304 56-57 0.4723143951345392 2304 58-59 0.3432216975643385 2304 60-61 0.40729956013063173 2304 62-63 0.43761827987943747 2304 64-65 0.4751972658243062 2304 66-67 0.5666107688119837 2304 68-69 0.44918670244131675 2304 70-71 0.46170453715414084 2304 72-73 0.32837444265210536 2304 74-75 0.522625352097073 2304 76-77 0.5464513248525193 2304 78-79 0.6215174910949344 2304 80-81 0.5716613253445857 2304 82-83 0.5027779111753325 2304 84-85 0.5893117659933331 2304 86-87 0.49123968187247513 2304 88-89 0.44766743849719504 2304 90-91 0.36463336829715587 2304 92-93 0.39117046878387285 2304 94-95 0.41267314870512095 2304 96-97 0.3042967134886503 2304 98-99 0.3892035091533792 2304 100-101 0.5078406427397368 2202 1 0.10576808648515268 2202 2 0.059033141444992054 2202 3 0.08699503065542302 2202 4 0.19030189770928274 2202 5 0.13459842220272833 2202 6 0.20144961655697102 2202 7 0.26528441870852504 2202 8 0.20013395508792087 2202 9 0.23012761971961737 2202 10-11 0.2903695262724071 2202 12-13 0.24326214264424806 2202 14-15 0.3499353145331199 2202 16-17 0.32622316974726573 2202 18-19 0.33234962878494656 2202 20-21 0.36182278256105604 2202 22-23 0.2827683580627962 2202 24-25 0.2788500412654642 2202 26-27 0.3341662234964389 2202 28-29 0.276266672586722 2202 30-31 0.3391010006468136 2202 32-33 0.18885154239931268 2202 34-35 0.23985399052861567 2202 36-37 0.30921010791821857 2202 38-39 0.2885628984888058 2202 40-41 0.23708090721841302 2202 42-43 0.32386951943610853 2202 44-45 0.3049541165572336 2202 46-47 0.3735154303102135 2202 48-49 0.32790293148944727 2202 50-51 0.19151579738789337 2202 52-53 0.20835137421762795 2202 54-55 0.3859659806570477 2202 56-57 0.43958233219075993 2202 58-59 0.3274848774041388 2202 60-61 0.31373011639006876 2202 62-63 0.3939553881217961 2202 64-65 0.4354878208669888 2202 66-67 0.4519505707995677 2202 68-69 0.38485001259049767 2202 70-71 0.3850254070714314 2202 72-73 0.2733314241028353 2202 74-75 0.3307467026493853 2202 76-77 0.3787417248754039 2202 78-79 0.3817669522704392 2202 80-81 0.4257354682427419 2202 82-83 0.4152282561624894 2202 84-85 0.41463786793962143 2202 86-87 0.42376665640509614 2202 88-89 0.3675513274819586 2202 90-91 0.36914249597175797 2202 92-93 0.35921243756342847 2202 94-95 0.3548126855985281 2202 96-97 0.4829083244944812 2202 98-99 0.40744421023746114 2202 100-101 0.4583454802815865 1102 1 -0.058938717565123255 1102 2 -0.14917454217421167 1102 3 -0.08355216399170473 1102 4 -0.16555361421972492 1102 5 -0.14095734075278443 1102 6 -0.03895749638640211 1102 7 -0.03474740791993014 1102 8 0.07641126896736239 1102 9 -0.028104581856261746 1102 10-11 -0.0014447830989681165 1102 12-13 -0.005701089194488418 1102 14-15 -0.04195988584454824 1102 16-17 -0.013279369065962499 1102 18-19 0.024974520925525212 1102 20-21 -0.005286208605475906 1102 22-23 0.08921507205214851 1102 24-25 0.06180355305630769 1102 26-27 0.013206354282978339 1102 28-29 0.007754367881055657 1102 30-31 0.03861227738320139 1102 32-33 -0.05543261581265568 1102 34-35 0.0011481153577790337 1102 36-37 -0.011041121520932506 1102 38-39 -0.05194878120403956 1102 40-41 -0.04510351965463144 1102 42-43 0.04239755829727443 1102 44-45 -0.02953929470446326 1102 46-47 -0.046046165250025695 1102 48-49 0.06402311076740119 1102 50-51 0.05576507188710167 1102 52-53 0.08660146018870307 1102 54-55 0.1805581211073246 1102 56-57 0.2810522264740385 1102 58-59 0.20448700557550126 1102 60-61 0.16535166368728227 1102 62-63 0.26104012588380243 1102 64-65 0.19555990730977868 1102 66-67 0.27430134806551365 1102 68-69 0.20308409047739673 1102 70-71 0.20003774206665526 1102 72-73 0.30126039566772533 1102 74-75 0.32704102187415174 1102 76-77 0.3019144489324006 1102 78-79 0.2857902781350248 1102 80-81 0.18793366656222332 1102 82-83 0.2738085799053387 1102 84-85 0.28669007466120533 1102 86-87 0.23716686416895172 1102 88-89 0.13127010626770286 1102 90-91 0.09243086615536455 1102 92-93 0.10140068135473257 1102 94-95 0.0619556807581958 1102 96-97 0.1573777810611645 1102 98-99 0.19464964439315935 1102 100-101 0.06202401264805246 2305 1 0.12400580426264796 2305 2 0.035655080978830966 2305 3 0.041123985209637226 2305 4 0.03068048405162216 2305 5 0.047519309914271446 2305 6 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68-69 0.1084390575159162 1213 70-71 0.15796330445075313 1213 72-73 0.3116060664994862 1213 74-75 0.24527505812693562 1213 76-77 0.2117655154476168 1213 78-79 0.15243491788881158 1213 80-81 0.12290481919477259 1213 82-83 0.2250757038579998 1213 84-85 0.22170673744433955 1213 86-87 0.22848443764053528 1213 88-89 0.11912830149289633 1213 90-91 0.21241352375398037 1213 92-93 0.19117794860975224 1213 94-95 0.30744623163732854 1213 96-97 0.31425127012416354 1213 98-99 0.19919034381742762 1213 100-101 0.2814772961750762 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4184.0 3 1004.0 4 1054.0 5 1031.0 6 2837.0 7 10477.0 8 42307.0 9 99341.0 10 127310.0 11 86561.0 12 48833.0 13 33096.0 14 26392.0 15 23107.0 16 22294.0 17 22844.0 18 24518.0 19 27003.0 20 29689.0 21 33805.0 22 38199.0 23 43058.0 24 48855.0 25 57295.0 26 67843.0 27 82969.0 28 100646.0 29 122507.0 30 150541.0 31 192271.0 32 260067.0 33 368983.0 34 554450.0 35 904285.0 36 1644874.0 37 2590037.0 38 738513.0 39 1127.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.45696367721579 27.934909355090003 17.279128611897228 17.328998355796983 2 32.73242774174427 26.55721430476079 25.35277842040373 15.357579533091211 3 34.48254162937362 22.87343135546376 23.93158830705221 18.71243870811041 4 30.627021964331234 21.22608062921603 26.628054219639598 21.518843186813136 5 33.0180103515347 19.91882141756724 26.05116883738069 21.01199939351737 6 31.87384950424831 19.83043908905336 27.760447174433487 20.535264232264844 7 33.891711829462686 20.447709636686273 23.237606997026113 22.422971536824935 8 29.32046418391419 26.237171505994844 22.523134148483084 21.91923016160788 9 28.51850035005223 24.850959625531672 25.319207389447683 21.311332634968412 10-11 24.602028423625796 29.612728504351804 28.28997334719814 17.495269724824254 12-13 25.28569859579533 27.05956570867644 28.721597001134597 18.93313869439363 14-15 24.278787801467956 27.023045782478572 29.40534794834488 19.292818467708592 16-17 25.31880648468559 26.877004907891006 29.46089740664416 18.34329120077924 18-19 25.1852053275311 26.64541309887849 29.873401619493574 18.29597995409684 20-21 24.558455060774378 28.01107739185891 29.14926885259342 18.28119869477329 22-23 25.921865733645177 27.23012967278032 28.218908185059572 18.629096408514933 24-25 24.268569492348284 28.293998378837433 28.538832597551345 18.898599531262942 26-27 22.97144385419051 29.74942505573001 28.727561154429587 18.55156993564989 28-29 23.492414200636833 29.334061117634374 28.593747352623677 18.579777329105113 30-31 24.60599857276964 28.141187537906813 28.87211744133353 18.380696447990022 32-33 22.91584536512851 29.170068319699123 29.50520873430037 18.408877580871994 34-35 24.69558349622242 28.376473010964894 28.771311624821617 18.15663186799107 36-37 23.262047216937525 28.59446269363653 29.8832347381157 18.26025535131025 38-39 22.789991018480592 29.520941664400997 29.055932895841856 18.633134421276548 40-41 25.121664804406297 27.484789829356366 28.246287648015993 19.147257718221343 42-43 25.02576217208875 27.393807315457302 28.494525611074305 19.08590490137964 44-45 23.753165982702264 27.942800204826174 29.161850619478326 19.14218319299324 46-47 23.78858947039797 28.496795868873686 28.607591758784938 19.1070229019434 48-49 22.031734663914246 29.069509789068803 29.33041564149675 19.5683399055202 50-51 21.529893541316703 28.29754151545768 29.860374861721684 20.312190081503935 52-53 23.863756339903855 27.902375402262532 28.178281463694933 20.05558679413868 54-55 24.80521908727445 27.81463764266058 28.016925324842084 19.363217945222893 56-57 25.028164493191362 27.47787689809625 28.0814117134076 19.412546895304786 58-59 24.457468556126717 27.69305345934898 28.160783834323478 19.688694150200824 60-61 22.468364209901644 27.89948368271477 27.954360256604183 21.6777918507794 62-63 23.192372160311113 27.94335238380445 28.020490151532357 20.84378530435208 64-65 22.244729173644423 28.254334979415553 29.154243212858795 20.346692634081233 66-67 21.640548943070385 27.974498509554692 28.92240069376919 21.46255185360573 68-69 21.139753112007767 27.870052433215946 30.923869163898075 20.066325290878208 70-71 20.768966870465285 29.76411332675229 29.88449798384158 19.582421818940848 72-73 20.748099015547623 31.562852269420883 27.96817531626934 19.72087339876216 74-75 20.932953413652893 31.632883629050205 27.659807001468224 19.774355955828675 76-77 21.069965113873096 31.347194054451116 27.731604991225478 19.85123584045031 78-79 20.95356043193245 31.141995399299628 28.02859501152498 19.875849157242946 80-81 20.602120529004846 31.42487930075201 27.792049895237447 20.18095027500569 82-83 20.501796849904864 31.21921045967253 27.75685030689396 20.522142383528646 84-85 20.754915149830147 30.786687708178107 27.949046329471916 20.50935081251983 86-87 20.797491091480936 30.521149817646943 28.534500687236914 20.14685840363521 88-89 21.108083982532833 30.769392544471824 28.15650387079664 19.966019602198703 90-91 21.34401156076013 30.336469077981626 27.77162874628364 20.547890614974605 92-93 21.171320534840106 30.461934452343336 27.603174108098866 20.76357090471769 94-95 20.803945689560894 30.45016491192143 27.604691584302664 21.141197814215012 96-97 20.790728267008678 30.805941170692453 27.654763223518387 20.748567338780486 98-99 21.22690083633185 30.433871039141252 27.506326138632335 20.832901985894562 100-101 21.48066036691789 30.372644282358984 27.07193672977705 21.074758620946074 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2339.0 1 1823.5 2 935.5 3 565.0 4 597.0 5 764.0 6 1042.0 7 1379.0 8 1825.0 9 2296.5 10 2655.0 11 2901.0 12 2917.5 13 2620.5 14 2246.0 15 1959.5 16 1682.5 17 1487.5 18 1491.5 19 1703.5 20 2140.0 21 2741.0 22 3735.5 23 5274.5 24 7352.0 25 10020.5 26 13816.5 27 18973.0 28 25059.0 29 31847.0 30 39604.5 31 49438.0 32 66444.5 33 95615.5 34 138438.5 35 199909.0 36 273635.5 37 356718.0 38 443995.0 39 494694.0 40 530525.5 41 536859.5 42 505119.5 43 499122.0 44 492891.0 45 487603.5 46 507913.5 47 487119.0 48 408876.0 49 334390.0 50 276950.5 51 231315.0 52 193273.5 53 159848.5 54 134918.0 55 113127.0 56 89171.0 57 67461.5 58 52260.5 59 41736.0 60 33521.5 61 26563.5 62 20785.0 63 16453.0 64 13392.5 65 10956.0 66 8904.5 67 7432.0 68 6231.5 69 5050.5 70 4122.5 71 3383.5 72 2760.5 73 2212.5 74 1754.5 75 1376.5 76 1090.0 77 892.5 78 673.5 79 518.0 80 428.0 81 339.5 82 254.5 83 195.0 84 156.0 85 132.0 86 115.0 87 90.0 88 72.5 89 66.5 90 61.5 91 53.0 92 39.0 93 30.0 94 30.5 95 27.0 96 16.0 97 14.0 98 10.0 99 5.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2985914050937162 2 0.2745243425366105 3 0.24458528733443619 4 0.2323201192651508 5 0.2386438036521478 6 0.18742890922119426 7 0.15256757221595452 8 0.15020487695048312 9 0.12980925752648736 10-11 0.11550568569875612 12-13 0.13604028719719136 14-15 0.13923687490929973 16-17 0.13273367200948508 18-19 0.12045113118089479 20-21 0.12598145955963297 22-23 0.1560015876385637 24-25 0.5676259556899667 26-27 0.22537101554317612 28-29 0.199033912436892 30-31 0.30027077182652673 32-33 0.33250882217672106 34-35 0.3408014192849442 36-37 0.3318312845638285 38-39 0.3395274169359155 40-41 0.34190169404092347 42-43 0.35332717874380354 44-45 0.33426926178628796 46-47 0.3538078250845735 48-49 0.3491403437513138 50-51 0.3548038632847232 52-53 0.33917996174981674 54-55 0.33171546616846226 56-57 0.34051766421629687 58-59 0.3325725222941725 60-61 0.30933935218370373 62-63 0.29630399178523287 64-65 0.3068724203624027 66-67 0.3229769682380791 68-69 0.310179035550109 70-71 0.2911269095123617 72-73 0.28783187616419204 74-75 0.2796029791734203 76-77 0.28187301972259876 78-79 0.2905072810971523 80-81 0.3003228901044415 82-83 0.32308120479390867 84-85 0.3137983604053042 86-87 0.31591783704050647 88-89 0.3216798022099771 90-91 0.3145685527344897 92-93 0.29667461065040485 94-95 0.2920650385148283 96-97 0.3093683067825453 98-99 0.31799098631756223 100-101 0.31928236142589583 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8634207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.38875796791105 #Duplication Level Percentage of deduplicated Percentage of total 1 90.04003232040675 46.2704542833627 2 4.598017703239394 4.725728377678789 3 1.620093999699345 2.497638553074438 4 0.938615781124817 1.9293719680433998 5 0.429942192412945 1.1047097633050937 6 0.34714404314945996 1.07035807280458 7 0.22651125112658604 0.8148092302806994 8 0.22662425077648563 0.9316751018249596 9 0.16879331437048456 0.7806670900907717 >10 1.1143887516050701 11.748794229420689 >50 0.15988075913309446 5.736334097281076 >100 0.11000789103512643 10.078215657303103 >500 0.01071624347663089 3.95140432761639 >1k 0.009118406564029811 7.9690944014171565 >5k 9.054536433286063E-5 0.2594132936674504 >10k+ 2.2546515561691203E-5 0.13133155282872908 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 11335 0.1312801511476387 Illumina Single End PCR Primer 1 (97% over 34bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0029417872423026224 0.0 0.0 2 0.003300824267937982 0.0 1.1581839536624498E-5 3 0.003787261528476211 0.0 1.1581839536624498E-5 4 0.0039609891215255785 0.0 1.1581839536624498E-5 5 0.0046327358146498 0.0 1.1581839536624498E-5 6 0.005014936519358408 0.0 1.1581839536624498E-5 7 0.005709846891555878 0.0 1.1581839536624498E-5 8 0.0063931754242167235 0.0 1.1581839536624498E-5 9 0.00715757683363394 0.0 1.1581839536624498E-5 10-11 0.010551055817864918 0.0 1.1581839536624498E-5 12-13 0.013915580203254334 0.0 1.1581839536624498E-5 14-15 0.018038715078292654 0.0 1.1581839536624498E-5 16-17 0.022040240638196422 0.0 1.1581839536624498E-5 18-19 0.02755319625762968 0.0 2.8954598841561247E-5 20-21 0.0338479260457851 0.0 4.053643837818574E-5 22-23 0.04132979438644452 0.0 4.632735814649799E-5 24-25 0.050045128637754455 0.0 4.632735814649799E-5 26-27 0.059559609817091484 0.0 4.632735814649799E-5 28-29 0.0702033203512494 0.0 4.632735814649799E-5 30-31 0.10422497399008386 0.0 5.211827791481024E-5 32-33 0.11877176444808424 0.0 5.790919768312249E-5 34-35 0.13272788108971675 0.0 5.790919768312249E-5 36-37 0.14737311718377843 0.0 5.790919768312249E-5 38-39 0.16406833887582264 0.0 5.790919768312249E-5 40-41 0.18094307908068452 0.0 5.790919768312249E-5 42-43 0.199311876585771 0.0 5.790919768312249E-5 44-45 0.2189430946003495 0.0 5.790919768312249E-5 46-47 0.23957035081507774 0.0 5.790919768312249E-5 48-49 0.2598791064425488 0.0 5.790919768312249E-5 50-51 0.28190776524120864 0.0 5.790919768312249E-5 52-53 0.30422017910851573 0.0 5.790919768312249E-5 54-55 0.32741860370037457 0.0 5.790919768312249E-5 56-57 0.3522442767471292 0.0 5.790919768312249E-5 58-59 0.37739424130090926 0.0 5.790919768312249E-5 60-61 0.40400351763630404 0.0 6.370011745143474E-5 62-63 0.43232111530335093 0.0 8.107287675637148E-5 64-65 0.4616637057693891 0.0 9.265471629299598E-5 66-67 0.49353692817417977 0.0 9.265471629299598E-5 68-69 0.527222708466452 0.0 9.265471629299598E-5 70-71 0.562848446881109 0.0 9.265471629299598E-5 72-73 0.6020298100335097 0.0 9.844563606130823E-5 74-75 0.6418539652801931 0.0 1.0423655582962049E-4 76-77 0.6863629746194411 0.0 1.0423655582962049E-4 78-79 0.7327656147229271 0.0 1.0423655582962049E-4 80-81 0.785063411150555 0.0 1.0423655582962049E-4 82-83 0.8426656900859569 0.0 1.0423655582962049E-4 84-85 0.9034066475357841 0.0 1.1002747559793274E-4 86-87 0.9720927469077357 0.0 1.1581839536624499E-4 88-89 1.046060165108388 0.0 1.1581839536624499E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 4090 0.0 81.70781 9 CGGGAGA 5885 0.0 77.23916 4 CGGAAAG 2495 0.0 75.68869 5 AGAGCGG 4705 0.0 73.45111 8 GATCGGG 7930 0.0 69.68193 1 ATTCGGA 995 0.0 69.64451 2 TTCGGAG 1150 0.0 68.49223 3 AAGCGGC 1500 0.0 67.94303 9 GAGAGCG 5800 0.0 67.2686 7 ATCGGGA 7985 0.0 66.87053 2 GTTCGGA 1305 0.0 65.1028 2 GATCCGG 1410 0.0 63.296417 1 TCGGGAA 2120 0.0 62.66907 3 TCGGAAA 2090 0.0 60.61547 4 GGACCGG 1040 0.0 59.796852 1 ACCGGAA 710 0.0 56.805668 3 AAAGCGG 1945 0.0 56.545605 8 TCGGAGA 2220 0.0 54.71488 4 GAACCGG 705 0.0 54.542656 1 AAGGGGC 5040 0.0 53.045223 9 >>END_MODULE