FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_30484-1.34200000035803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_30484-1.34200000035803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8634207
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT113350.1312801511476387Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC40900.081.707819
CGGGAGA58850.077.239164
CGGAAAG24950.075.688695
AGAGCGG47050.073.451118
GATCGGG79300.069.681931
ATTCGGA9950.069.644512
TTCGGAG11500.068.492233
AAGCGGC15000.067.943039
GAGAGCG58000.067.26867
ATCGGGA79850.066.870532
GTTCGGA13050.065.10282
GATCCGG14100.063.2964171
TCGGGAA21200.062.669073
TCGGAAA20900.060.615474
GGACCGG10400.059.7968521
ACCGGAA7100.056.8056683
AAAGCGG19450.056.5456058
TCGGAGA22200.054.714884
GAACCGG7050.054.5426561
AAGGGGC50400.053.0452239
Produced by FastQC (version 0.11.2)