##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_30423-1.34200000035748.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2724480 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.78286608820766 33.0 33.0 33.0 22.0 33.0 2 30.181065377613344 33.0 33.0 33.0 22.0 33.0 3 30.015720064012214 33.0 33.0 33.0 15.0 33.0 4 33.34361125792812 37.0 37.0 37.0 15.0 37.0 5 33.44143653100775 37.0 37.0 37.0 15.0 37.0 6 34.07163752349072 37.0 37.0 37.0 27.0 37.0 7 33.58941632898755 37.0 37.0 37.0 22.0 37.0 8 33.675959816185106 37.0 37.0 37.0 22.0 37.0 9 33.1183407475922 37.0 37.0 37.0 15.0 37.0 10-11 33.16516527924595 37.0 37.0 37.0 15.0 37.0 12-13 33.13338343463707 37.0 37.0 37.0 15.0 37.0 14-15 34.63515202901104 40.0 37.0 40.0 15.0 40.0 16-17 34.507895635130375 40.0 37.0 40.0 15.0 40.0 18-19 34.62802773373268 40.0 37.0 40.0 15.0 40.0 20-21 34.580239715468636 40.0 37.0 40.0 15.0 40.0 22-23 35.4188680041696 40.0 37.0 40.0 24.5 40.0 24-25 34.71214084889594 37.0 37.0 40.0 18.5 40.0 26-27 34.41206266883955 37.0 37.0 40.0 18.5 40.0 28-29 34.20503986081748 37.0 37.0 40.0 15.0 40.0 30-31 34.263558734143764 37.0 37.0 40.0 15.0 40.0 32-33 34.0351804748062 37.0 37.0 40.0 10.5 40.0 34-35 34.24052846781771 37.0 37.0 40.0 15.0 40.0 36-37 33.89440755667137 37.0 33.0 40.0 15.0 40.0 38-39 33.75379889006342 37.0 33.0 40.0 10.5 40.0 40-41 34.27682291666667 37.0 33.0 40.0 18.5 40.0 42-43 34.04056920953723 37.0 33.0 40.0 15.0 40.0 44-45 33.52067954251821 37.0 33.0 40.0 10.5 40.0 46-47 33.29223062749589 37.0 33.0 40.0 6.0 40.0 48-49 33.146789846135775 37.0 33.0 40.0 6.0 40.0 50-51 32.187630116572706 37.0 33.0 38.5 15.0 38.5 52-53 32.63938714910735 37.0 33.0 38.5 15.0 40.0 54-55 33.32805067389006 37.0 33.0 40.0 15.0 40.0 56-57 33.66601167929293 37.0 33.0 40.0 18.5 40.0 58-59 33.13481361580925 37.0 33.0 40.0 15.0 40.0 60-61 32.86940315216115 37.0 33.0 40.0 10.5 40.0 62-63 32.501644166960304 37.0 33.0 38.5 6.0 40.0 64-65 32.264609393352124 37.0 33.0 37.0 6.0 40.0 66-67 32.03434600364106 37.0 33.0 37.0 10.5 40.0 68-69 32.10932911968523 37.0 33.0 37.0 15.0 38.5 70-71 31.75299433286352 37.0 33.0 37.0 15.0 37.0 72-73 31.96970504463237 37.0 33.0 37.0 15.0 37.0 74-75 32.14296122562838 37.0 33.0 37.0 22.0 37.0 76-77 31.870810026133427 35.0 33.0 37.0 22.0 37.0 78-79 31.51700214352831 33.0 33.0 37.0 18.5 37.0 80-81 31.043442418369743 33.0 33.0 37.0 15.0 37.0 82-83 30.577862748120744 33.0 27.0 37.0 15.0 37.0 84-85 30.294565568475452 33.0 27.0 37.0 15.0 37.0 86-87 30.155267060136246 33.0 27.0 37.0 15.0 37.0 88-89 30.101624163143057 33.0 27.0 37.0 6.0 37.0 90-91 30.01196209918957 33.0 27.0 37.0 6.0 37.0 92-93 29.803151977625088 33.0 27.0 37.0 6.0 37.0 94-95 29.59749493481325 33.0 27.0 37.0 6.0 37.0 96-97 29.45975966055908 33.0 27.0 37.0 6.0 37.0 98-99 29.328131973807846 33.0 27.0 37.0 6.0 37.0 100-101 28.051706931230914 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.0881951661588154 2306 2 -0.02765144587446855 2306 3 -0.1423953685555439 2306 4 0.06757361107969473 2306 5 -0.02794976802261573 2306 6 -0.20405045866922933 2306 7 0.19082045912701773 2306 8 -0.2689101819141726 2306 9 0.07544860303070067 2306 10-11 -0.015606187320749143 2306 12-13 0.004070195065651205 2306 14-15 0.29594266335279684 2306 16-17 0.259616623203776 2306 18-19 0.18086770794823082 2306 20-21 0.21717162903305365 2306 22-23 -0.014932250259832358 2306 24-25 0.40344738776116884 2306 26-27 -0.11644112575106647 2306 28-29 0.030906878524767478 2306 30-31 0.009406149238294859 2306 32-33 0.1520541347663169 2306 34-35 0.005157590137073953 2306 36-37 0.11494830717651183 2306 38-39 0.0530297827592463 2306 40-41 -0.2966543052539379 2306 42-43 0.16378745610406042 2306 44-45 0.40015374496466194 2306 46-47 0.4945113969549979 2306 48-49 0.35279955064118695 2306 50-51 0.09969006165223249 2306 52-53 0.02488721628211721 2306 54-55 0.2792937516214167 2306 56-57 0.2440086692464618 2306 58-59 0.39821078419751643 2306 60-61 0.3682107745087322 2306 62-63 0.2741947299986123 2306 64-65 0.19005571046783842 2306 66-67 0.19879995935512085 2306 68-69 -0.07710295871714123 2306 70-71 0.183668584438216 2306 72-73 -0.10948547980741807 2306 74-75 -0.0835280393616955 2306 76-77 0.01500915127430602 2306 78-79 0.171856014311782 2306 80-81 0.39656905127937137 2306 82-83 0.45334027551069767 2306 84-85 0.41958968565814914 2306 86-87 0.33984605724247174 2306 88-89 0.3364497411003313 2306 90-91 0.27373450215338835 2306 92-93 0.27286454897149426 2306 94-95 0.19442650816212748 2306 96-97 0.1230734328229417 2306 98-99 0.29428819412194684 2306 100-101 0.3036385074362613 2307 1 0.17781646757465452 2307 2 0.26714986564380183 2307 3 0.12942768540841953 2307 4 0.30102713061085495 2307 5 0.23172158989986968 2307 6 -0.140417858072297 2307 7 0.40248088046860175 2307 8 -0.07266609137148095 2307 9 0.3031293725128421 2307 10-11 0.2791926732705221 2307 12-13 0.2583239150281784 2307 14-15 0.5524163785224019 2307 16-17 0.5043572061976178 2307 18-19 0.6008460308605876 2307 20-21 0.4002782062317962 2307 22-23 0.2588055223377097 2307 24-25 0.6619602741906618 2307 26-27 0.298611650876893 2307 28-29 0.3938297995940303 2307 30-31 0.36444525514013293 2307 32-33 0.5298868617221544 2307 34-35 0.22531891559840034 2307 36-37 0.5280343510535488 2307 38-39 0.23756770132311544 2307 40-41 0.06899214035727397 2307 42-43 0.3810980811449127 2307 44-45 0.44966982304574543 2307 46-47 0.4819275207867193 2307 48-49 0.5318778931609529 2307 50-51 0.2650748027254082 2307 52-53 0.24346960010817753 2307 54-55 0.2853734121952627 2307 56-57 0.07301702418426714 2307 58-59 0.49561988203851826 2307 60-61 0.3143410331087608 2307 62-63 0.44613624543400476 2307 64-65 0.5708954525213912 2307 66-67 0.599694003057877 2307 68-69 0.3424426394179676 2307 70-71 0.45633666356503255 2307 72-73 0.10034518836974371 2307 74-75 0.23073499412340936 2307 76-77 0.2939121163513221 2307 78-79 0.3580725455420364 2307 80-81 0.6543202440638431 2307 82-83 0.7640546390321887 2307 84-85 0.5709058334544963 2307 86-87 0.5361326305197416 2307 88-89 0.45619549827337735 2307 90-91 0.3935507915588481 2307 92-93 0.30259756132416626 2307 94-95 0.2989481961427778 2307 96-97 0.3722414792707305 2307 98-99 0.31664500552039243 2307 100-101 0.2089801666593729 2201 1 0.10000322260324168 2201 2 0.15119788205772977 2201 3 0.03708566238760014 2201 4 0.2352503956945995 2201 5 0.11168941624924855 2201 6 -0.02819794706884693 2201 7 0.3716341271008474 2201 8 0.05786353329963845 2201 9 0.33939409978115265 2201 10-11 0.2930688892765332 2201 12-13 0.2268616899523721 2201 14-15 0.6209491911901281 2201 16-17 0.5191339704823292 2201 18-19 0.5478301435382349 2201 20-21 0.512427987386161 2201 22-23 0.28284600928698467 2201 24-25 0.5421238058589637 2201 26-27 0.271323670879859 2201 28-29 0.3249301343944353 2201 30-31 0.3824521698926162 2201 32-33 0.4397621571789543 2201 34-35 0.24757691445201147 2201 36-37 0.4822212043145626 2201 38-39 0.2740319315556121 2201 40-41 0.08451881299215103 2201 42-43 0.51840700895319 2201 44-45 0.6500673858689225 2201 46-47 0.5129207366732302 2201 48-49 0.4427160182786807 2201 50-51 0.3459400886020205 2201 52-53 0.5121266717287298 2201 54-55 0.5390992819572986 2201 56-57 0.5259992643696307 2201 58-59 0.5918028434726708 2201 60-61 0.5674016820098728 2201 62-63 0.6061359770670691 2201 64-65 0.6106955343479896 2201 66-67 0.3976935807597215 2201 68-69 0.2751439348707265 2201 70-71 0.3389027122113504 2201 72-73 0.18211241499498243 2201 74-75 0.2686910660011961 2201 76-77 0.31652911926993 2201 78-79 0.3922895824875532 2201 80-81 0.5889044120352125 2201 82-83 0.6849061419882787 2201 84-85 0.5726057860598814 2201 86-87 0.527208164164854 2201 88-89 0.69930910153996 2201 90-91 0.5611863219227935 2201 92-93 0.5988262970424891 2201 94-95 0.5902792353608071 2201 96-97 0.5023443752030943 2201 98-99 0.5350799908462918 2201 100-101 0.5814930887803023 1101 1 0.16048064731828404 1101 2 0.1471749410603742 1101 3 0.1228505035616898 1101 4 0.18266022064445053 1101 5 0.15943753824546292 1101 6 0.10742432382201628 1101 7 0.5598976367469675 1101 8 0.14095171550498264 1101 9 0.274879084645157 1101 10-11 0.2691198023018586 1101 12-13 0.23642075276596586 1101 14-15 0.5124631986550554 1101 16-17 0.4347097608448962 1101 18-19 0.48561069307996974 1101 20-21 0.4202735908325437 1101 22-23 0.34500393216613645 1101 24-25 0.547578309458018 1101 26-27 0.27148868617280897 1101 28-29 0.41493954141716216 1101 30-31 0.5044926462500143 1101 32-33 0.4463566998501065 1101 34-35 0.30927421759777474 1101 36-37 0.48758216088006634 1101 38-39 0.33529592567909816 1101 40-41 0.28803791845648874 1101 42-43 0.5715496765504469 1101 44-45 0.6500327504459946 1101 46-47 0.5341970363125412 1101 48-49 0.4783085717673927 1101 50-51 0.3487446161110199 1101 52-53 0.31454246631869154 1101 54-55 0.5211409721240798 1101 56-57 0.49730365951062083 1101 58-59 0.6825428381779588 1101 60-61 0.6082779686739812 1101 62-63 0.5810632793024695 1101 64-65 0.6371552186110492 1101 66-67 0.4919809333891365 1101 68-69 0.4686824926768267 1101 70-71 0.4140342692671908 1101 72-73 0.1258910663899755 1101 74-75 0.18109316338009762 1101 76-77 0.3317919351612133 1101 78-79 0.561417282559443 1101 80-81 0.569481473758465 1101 82-83 0.6147283921606714 1101 84-85 0.6295562574050848 1101 86-87 0.6596373839051637 1101 88-89 0.5788304630155352 1101 90-91 0.6020815481988357 1101 92-93 0.45024870375443626 1101 94-95 0.6243909414012876 1101 96-97 0.5112902550003895 1101 98-99 0.6779122894768861 1101 100-101 0.6142325623846574 2304 1 0.029950678581474932 2304 2 -0.010607649696734 2304 3 0.034011586653555526 2304 4 -0.007712317295876403 2304 5 -0.055618341969157825 2304 6 0.18221014571235372 2304 7 0.3139200688726831 2304 8 0.05360306470082321 2304 9 0.10959771116102957 2304 10-11 0.04533424054109503 2304 12-13 0.18861370220649576 2304 14-15 0.17805291620008035 2304 16-17 0.2643046924155712 2304 18-19 0.2564823748795746 2304 20-21 0.2564808635979361 2304 22-23 0.016328125073655997 2304 24-25 0.24443674465955212 2304 26-27 0.12581126780155927 2304 28-29 0.14705029753663723 2304 30-31 0.23943119183034156 2304 32-33 0.2604006474544889 2304 34-35 0.10426724745931182 2304 36-37 0.1610000633209978 2304 38-39 -0.020947517483541844 2304 40-41 0.14064574121010054 2304 42-43 0.2494860200815623 2304 44-45 0.16619441954402703 2304 46-47 0.09483457291410247 2304 48-49 0.08022686059124595 2304 50-51 0.12940098113855925 2304 52-53 0.005949519546156523 2304 54-55 0.35130647360720246 2304 56-57 0.31034863334044616 2304 58-59 0.21540520676726516 2304 60-61 0.1637407372720574 2304 62-63 0.2126727381090916 2304 64-65 0.35173629089753433 2304 66-67 0.4693477551489309 2304 68-69 0.26115636299509504 2304 70-71 0.2062138977869239 2304 72-73 0.02187687646490133 2304 74-75 0.43090788061544316 2304 76-77 0.3439150212317017 2304 78-79 0.4075189122283156 2304 80-81 0.42933852636657477 2304 82-83 0.24075888163304882 2304 84-85 0.3342278223845945 2304 86-87 0.36481339062088836 2304 88-89 0.1781214431894682 2304 90-91 0.40002671517248345 2304 92-93 0.41403638085455796 2304 94-95 0.37219913538743654 2304 96-97 0.35939326249840775 2304 98-99 0.515040497146039 2304 100-101 0.2805632204171822 2202 1 -0.01678142424275464 2202 2 -0.1863198149691705 2202 3 -0.07114148878433468 2202 4 -0.02068793999833929 2202 5 -0.2049379236909772 2202 6 -0.035559910259031824 2202 7 0.08316472329401847 2202 8 0.10480391398235156 2202 9 -0.14001442625313842 2202 10-11 -0.0482870962512294 2202 12-13 -0.020707918353423338 2202 14-15 -0.033604843732000234 2202 16-17 -0.11929610659804979 2202 18-19 -0.08662006882608608 2202 20-21 -0.010098361520554988 2202 22-23 0.014826503230665367 2202 24-25 0.006729752060010696 2202 26-27 0.1283316935320471 2202 28-29 -0.0346734522030161 2202 30-31 0.10873815384730534 2202 32-33 -0.08915085210215068 2202 34-35 -0.02955066144856744 2202 36-37 0.11862202231047547 2202 38-39 0.06360248370285149 2202 40-41 0.06059243262404124 2202 42-43 0.20898432529737931 2202 44-45 -0.03053667264924087 2202 46-47 0.0351324717683994 2202 48-49 0.19146542837778213 2202 50-51 0.07604863036700493 2202 52-53 0.019347058703708342 2202 54-55 0.257865995816033 2202 56-57 0.37078454044324616 2202 58-59 0.2507457410658773 2202 60-61 0.22168829519218747 2202 62-63 0.19013786474994276 2202 64-65 0.23007574513923856 2202 66-67 0.22328741432901467 2202 68-69 0.13997011073984567 2202 70-71 0.23954767052502035 2202 72-73 0.29438235364528964 2202 74-75 0.43932186669334783 2202 76-77 0.532967690923229 2202 78-79 0.4850534648219913 2202 80-81 0.3826750585665941 2202 82-83 0.26835745550094003 2202 84-85 0.30368963064065824 2202 86-87 0.35690770797482685 2202 88-89 0.37514676081320175 2202 90-91 0.25316366972836235 2202 92-93 0.2643446232970099 2202 94-95 0.2941332545522535 2202 96-97 0.18975982274124448 2202 98-99 0.0824765615557439 2202 100-101 0.26716242966436354 1102 1 -0.04011656054562707 1102 2 -0.2213334834274363 1102 3 -0.13854250007157276 1102 4 -0.19611362760381468 1102 5 -0.17335200894382297 1102 6 0.006258262699354589 1102 7 -0.0176303700299556 1102 8 0.16697842519081263 1102 9 -0.07240582982431931 1102 10-11 -0.13190386939055543 1102 12-13 -0.07328251012701514 1102 14-15 -0.1202034198733557 1102 16-17 -0.16640152833861777 1102 18-19 -0.06461799297466797 1102 20-21 -0.18634659306732004 1102 22-23 -0.06401459342961857 1102 24-25 -0.10577554720857307 1102 26-27 -0.08523857598753892 1102 28-29 -0.16364181252404109 1102 30-31 -0.06232832699978985 1102 32-33 -0.14621175186516666 1102 34-35 -0.1025935055274374 1102 36-37 -0.17704679005237267 1102 38-39 -0.09994171585176304 1102 40-41 0.003615427795011783 1102 42-43 -0.1637962760498084 1102 44-45 -0.15523345702870728 1102 46-47 -0.16101006678022856 1102 48-49 0.0015942188775923682 1102 50-51 -0.05087823241726852 1102 52-53 -0.032697362567425614 1102 54-55 0.10540161258592207 1102 56-57 0.18908029566820517 1102 58-59 0.1270661726090765 1102 60-61 0.050608576325494425 1102 62-63 0.1395511687159825 1102 64-65 0.07283129982850767 1102 66-67 0.13546712328750488 1102 68-69 0.17867775516826612 1102 70-71 0.12076373287203168 1102 72-73 0.18597905372374157 1102 74-75 0.3732477435294044 1102 76-77 0.2830806607714038 1102 78-79 0.31956993600452677 1102 80-81 0.280938847628903 1102 82-83 0.23590197341774655 1102 84-85 0.15954424632389674 1102 86-87 0.1331747001505903 1102 88-89 0.06980753453078137 1102 90-91 -0.09230917121561433 1102 92-93 0.14260828241557277 1102 94-95 0.14200778086667398 1102 96-97 0.09105422492689996 1102 98-99 0.058799348329422685 1102 100-101 0.062303832829474715 2305 1 -0.08005621488702275 2305 2 -0.3430229647609053 2305 3 -0.3456553973315053 2305 4 -0.48833087320670643 2305 5 -0.39366740540223333 2305 6 0.029145818170455584 2305 7 -0.3622637056320812 2305 8 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62-63 0.15327900962856233 1213 64-65 0.21897181140862898 1213 66-67 0.39300786851064373 1213 68-69 0.3725035982929086 1213 70-71 0.26683210673238733 1213 72-73 0.6594157341013229 1213 74-75 0.6164140355323866 1213 76-77 0.2742663947108639 1213 78-79 0.29363571422055657 1213 80-81 0.24254647447963862 1213 82-83 0.22147629037534244 1213 84-85 0.2808954350747008 1213 86-87 0.30212058306811684 1213 88-89 0.23749533123683264 1213 90-91 0.2266955384251368 1213 92-93 0.2314668469471961 1213 94-95 0.4130600633085031 1213 96-97 0.393715198223461 1213 98-99 0.3624582085052843 1213 100-101 0.5649960256672806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1297.0 3 446.0 4 496.0 5 386.0 6 888.0 7 4987.0 8 25650.0 9 68830.0 10 85699.0 11 53380.0 12 25472.0 13 14866.0 14 10394.0 15 8602.0 16 7863.0 17 7584.0 18 7539.0 19 7922.0 20 8487.0 21 9462.0 22 10587.0 23 11425.0 24 12981.0 25 15423.0 26 18250.0 27 22711.0 28 27954.0 29 33856.0 30 41749.0 31 55557.0 32 77114.0 33 111552.0 34 171670.0 35 295495.0 36 553607.0 37 767087.0 38 147157.0 39 55.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.299552410130445 20.467057341423672 24.473645652252344 11.759744596193535 2 35.56218305592791 22.74246014482352 31.26090832334055 10.434448475908017 3 40.03772228348598 17.1606217921617 28.82097258879289 13.980683335559435 4 31.740962272994505 14.504143694175282 35.63941214948939 18.115481883340827 5 34.930626610345286 12.91668460282718 35.41495813240235 16.737730654425192 6 31.35972734700962 13.095316019907669 40.92243966765636 14.622516965426346 7 40.268342391304344 14.736260116694899 28.776776891586675 16.21862060041408 8 31.47782588461564 31.395556575038214 21.624807836096597 15.501809704249553 9 35.94612126390842 28.31316555950642 19.51299649935224 16.227716677232927 10-11 31.473352619263945 28.21738655123281 24.754168691566893 15.555092137936358 12-13 33.83328913580696 21.869983557785094 24.724665950317572 19.572061356090373 14-15 30.005825781564432 22.54671192520946 25.63990789016656 21.807554403059545 16-17 30.414803960321805 24.8604704540639 26.96998481534835 17.754740770265943 18-19 30.09491582851025 25.02441477575798 26.92124303225727 17.959426363474503 20-21 28.09948866855186 27.605246315416547 26.682361683529436 17.612903332502153 22-23 31.943740601514126 24.092382670225984 25.71570946172605 18.248167266533844 24-25 29.327312166599395 26.600475174591505 26.027833131145123 18.044379527663978 26-27 24.01078810836005 29.496257582917075 27.391001976005047 19.10195233271783 28-29 25.332542882392595 28.888192994579505 25.425458870378424 20.353805252649476 30-31 27.124033467677215 26.631011790553128 26.30002370774389 19.944931034025768 32-33 24.690861110937217 26.42010188077673 28.59341851508013 20.29561849320592 34-35 29.281463457211792 24.408944380832985 26.107358055500214 20.202234106455013 36-37 24.92380878029236 27.85162855509925 28.349891144878946 18.87467151972944 38-39 23.217598952306524 30.496192314803018 24.896678007349472 21.38953072554099 40-41 29.61109482427155 25.103785634418234 23.852557369369315 21.4325621719409 42-43 30.94343563149677 25.513065456754134 24.49986316290024 19.043635748848857 44-45 25.947238638439284 28.42452435525021 25.19926554204941 20.428971464261096 46-47 26.220656957078887 28.894188428276284 25.670804678311736 19.21434993633309 48-49 23.267101728239005 27.67942974963702 28.22627695285998 20.827191569264 50-51 20.388233110041927 26.468890593103527 28.90161401895804 24.241262277896507 52-53 25.950849898602158 26.96121912374514 25.558185309181013 21.52974566847169 54-55 28.070343738561014 26.55719364506053 25.779195240350372 19.59326737602808 56-57 29.37121180732986 25.900295091174424 23.916564236422815 20.811928865072897 58-59 26.831967802478264 24.70030714547309 25.04866067376091 23.41906437828774 60-61 22.32084343967636 24.736627596170415 26.726086072907044 26.216442891246178 62-63 25.018415382559557 24.817769620159037 26.702304711356135 23.461510285925275 64-65 23.87400795568321 27.695747488698707 27.44169504760653 20.988549508011552 66-67 21.539625125978603 27.493345273606927 26.807473030361166 24.159556570053308 68-69 20.213504657413203 24.173299952137256 33.54738411693236 22.065811273517173 70-71 19.71738545471545 30.15578475266994 30.179858670641195 19.946971121973416 72-73 21.471366848428232 34.19357201593377 25.48418217971058 18.85087895592742 74-75 22.485169096954614 33.44244417109993 25.15414275208855 18.918243979856914 76-77 21.5435402597025 32.36336118537319 25.173813770132426 20.919284784791877 78-79 21.727833006891174 33.54630294593036 25.083346738259397 19.642517308919068 80-81 21.064939783718735 34.07798472730526 24.75726752506473 20.099807963911278 82-83 20.639274080573802 33.036764528542136 25.710020699367238 20.61394069151683 84-85 21.20661911342731 31.77911178521257 25.9224861457484 21.09178295561172 86-87 19.570166797344807 32.15260310302745 27.04551483720032 21.231715262427418 88-89 19.41583289838383 32.18860146934688 27.087716197519544 21.307849434749752 90-91 20.920794175311347 30.90523835384431 25.996852017194243 22.177115453650096 92-93 21.13596121494123 31.27768717950606 24.969869428052906 22.616482177499805 94-95 21.465492362556944 32.41450024589415 24.781400831033903 21.338606560514993 96-97 20.78590948041393 33.435548998077245 23.748740175671283 22.02980134583754 98-99 21.183617097501443 31.03170222785647 25.325146570473677 22.45953410416841 100-101 22.599889170565028 31.646019621596643 24.856391194767085 20.897700013071248 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 797.0 1 644.0 2 350.5 3 256.0 4 302.5 5 321.0 6 402.5 7 522.5 8 650.0 9 808.0 10 939.0 11 1026.0 12 1047.0 13 959.5 14 827.0 15 703.0 16 626.0 17 590.5 18 589.5 19 594.0 20 582.5 21 601.0 22 662.0 23 728.0 24 851.5 25 1055.5 26 1288.0 27 1563.0 28 1919.5 29 2383.5 30 3139.0 31 4353.0 32 6769.5 33 10974.0 34 17746.5 35 29465.5 36 46546.5 37 68325.5 38 93760.5 39 112078.5 40 120453.5 41 122498.0 42 151998.0 43 189761.5 44 182130.0 45 162295.0 46 162351.5 47 155855.5 48 132992.5 49 116397.5 50 103292.5 51 97159.5 52 97511.0 53 100307.0 54 87899.5 55 59613.0 56 43547.0 57 37461.0 58 33262.0 59 28054.0 60 22750.5 61 18740.0 62 15275.5 63 12441.5 64 10388.0 65 8451.5 66 6830.5 67 5646.0 68 4666.5 69 3779.0 70 3061.0 71 2482.0 72 1905.0 73 1440.0 74 1085.0 75 801.5 76 618.5 77 474.0 78 352.0 79 269.0 80 202.5 81 158.5 82 109.5 83 83.0 84 67.5 85 44.0 86 35.0 87 37.5 88 28.5 89 16.0 90 16.0 91 16.0 92 8.0 93 4.0 94 2.5 95 1.0 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2992864693446089 2 0.27594256518675125 3 0.2467626849894292 4 0.2339529011040639 5 0.23883456659619448 6 0.18814599483204134 7 0.15503875968992248 8 0.15217582804792107 9 0.13048361522198731 10-11 0.11571015386422363 12-13 0.1372555496828753 14-15 0.14002672069532535 16-17 0.1336218287526427 18-19 0.12037159384543106 20-21 0.1265012038994597 22-23 0.15762640944326992 24-25 0.56416270260747 26-27 0.2245015562602772 28-29 0.19662467700258396 30-31 0.29612990368804326 32-33 0.328613166549213 34-35 0.33808286351891004 36-37 0.3282828282828283 38-39 0.33701844021611466 40-41 0.3401750058726803 42-43 0.3513698026779422 44-45 0.33063190039934226 46-47 0.3512229856706601 48-49 0.3472956307258633 50-51 0.3539023960535589 52-53 0.33714690509748646 54-55 0.3298244068592906 56-57 0.3383214411557435 58-59 0.3300262802443035 60-61 0.30699436222692034 62-63 0.29367071881606766 64-65 0.3049572762508809 66-67 0.3208318651632605 68-69 0.30758163025604884 70-71 0.2880182640357059 72-73 0.2838706835799859 74-75 0.27594256518675125 76-77 0.27761260864458537 78-79 0.28691713648108996 80-81 0.29596473455485084 82-83 0.3191985259572469 84-85 0.30965542048390887 86-87 0.31270187338501293 88-89 0.3174000176180409 90-91 0.3103344491425887 92-93 0.2925328870096312 94-95 0.2886422363166549 96-97 0.305654657035471 98-99 0.31385805731735966 100-101 0.31547304439746304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2724480.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.92316186563668 #Duplication Level Percentage of deduplicated Percentage of total 1 87.41937294479519 36.64896522157108 2 5.542925486947417 4.647539247969192 3 1.863312138057906 2.3434780911002147 4 1.1150291286448284 1.869821865803079 5 0.7595737244943873 1.592186610043136 6 0.48905038803024004 1.2301523146706508 7 0.29537707435140753 0.8668198629602583 8 0.29634920654505925 0.9939116603793213 9 0.21666821176312398 0.8175074861595143 >10 1.5649886614940256 13.18541789358289 >50 0.21977715622110797 6.493526960951139 >100 0.19399139956187134 15.705458724184613 >500 0.01568478205308363 4.478156718665425 >1k 0.0071074459291961675 4.805072975997639 >5k 3.5211160573058234E-4 0.8608245605475697 >10k+ 4.4013950556917304E-4 3.4611598054144688 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 32196 1.1817300916138125 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 24846 0.9119538407329104 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 12391 0.45480238430819825 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 12353 0.45340762273901813 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12002 0.44052443035001176 No Hit GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT 6590 0.24188101949729857 Illumina Single End PCR Primer 1 (97% over 34bp) TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6260 0.2297686163965234 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5245 0.19251380079868452 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5231 0.19199994127319708 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 3723 0.1366499295278365 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 3457 0.12688659854357529 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004991778247592201 0.0 0.0 2 0.0052120037585153866 0.0 0.0 3 0.005652454780361757 0.0 0.0 4 0.006129610054028659 0.0 0.0 5 0.006900399342259807 0.0 0.0 6 0.00785470988959361 0.0 0.0 7 0.00866220342964529 0.0 0.0 8 0.009396288466055908 0.0 0.0 9 0.010974571294338736 0.0 0.0 10-11 0.016700434578341555 0.0 0.0 12-13 0.022059255344139064 0.0 0.0 14-15 0.028298978153629317 0.0 0.0 16-17 0.03437353182992718 0.0 0.0 18-19 0.040301268498942916 0.0 0.0 20-21 0.04518293399107352 0.0 0.0 22-23 0.049862726098191215 0.0 0.0 24-25 0.056102448907681464 0.0 0.0 26-27 0.06171819943622269 0.0 0.0 28-29 0.0686185987784825 0.0 0.0 30-31 0.12127084801503407 0.0 0.0 32-33 0.1307038407329105 0.0 0.0 34-35 0.1384667899929528 0.0 0.0 36-37 0.14685371153394408 0.0 0.0 38-39 0.15623164787408972 0.0 0.0 40-41 0.16663730326521026 0.0 0.0 42-43 0.17819914258867747 0.0 0.0 44-45 0.19044001057082452 0.0 0.0 46-47 0.20396552736669016 0.0 0.0 48-49 0.21877569297627436 0.0 0.0 50-51 0.2347970988959361 0.0 0.0 52-53 0.2520848015034062 0.0 0.0 54-55 0.27205191449377497 0.0 0.0 56-57 0.2939643528306319 0.0 0.0 58-59 0.3184277366690157 0.0 0.0 60-61 0.3458825170307729 0.0 0.0 62-63 0.37572307376086445 0.0 0.0 64-65 0.406316067653277 0.0 0.0 66-67 0.4420660089264741 0.0 0.0 68-69 0.48115603711533944 0.0 0.0 70-71 0.524687279774489 0.0 0.0 72-73 0.5732653570589618 0.0 0.0 74-75 0.6232381959126145 0.0 0.0 76-77 0.6791754756871036 0.0 0.0 78-79 0.7440135365280713 0.0 0.0 80-81 0.8173302795395818 0.0 0.0 82-83 0.9011811428235847 0.0 0.0 84-85 0.9922260394644116 0.0 0.0 86-87 1.0951631136950906 0.0 0.0 88-89 1.207000968992248 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGGC 2560 0.0 86.87858 9 ATCGGGA 3975 0.0 86.72064 2 CGGGAGA 3485 0.0 83.49814 4 ATTCGGA 660 0.0 83.452065 2 GTTCGGA 705 0.0 80.81931 2 TCGGAGA 1065 0.0 80.6767 4 AGAGCGG 2930 0.0 79.31224 8 AAGCGGC 915 0.0 78.259254 9 ATCCGGA 765 0.0 77.5839 2 CGGAGAG 1850 0.0 76.97836 5 TCGGGAG 3905 0.0 75.858925 3 TTGGGAG 2255 0.0 71.78785 3 TCGGGAA 1230 0.0 70.24402 3 CGGAAAG 2005 0.0 68.65983 5 CCGGAGA 910 0.0 68.335915 4 AAGGGGC 2695 0.0 68.09763 9 GTCCGGA 400 0.0 66.473885 2 TCGGAAA 1465 0.0 66.42558 4 GATCCGG 960 0.0 65.29767 1 GGATCGG 1675 0.0 64.92555 1 >>END_MODULE