FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_30423-1.34200000035748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_30423-1.34200000035748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2724480
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA321961.1817300916138125No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC248460.9119538407329104No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT123910.45480238430819825No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC123530.45340762273901813No Hit
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT120020.44052443035001176No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT65900.24188101949729857Illumina Single End PCR Primer 1 (97% over 34bp)
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT62600.2297686163965234No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT52450.19251380079868452No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC52310.19199994127319708No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC37230.1366499295278365No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG34570.12688659854357529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGGC25600.086.878589
ATCGGGA39750.086.720642
CGGGAGA34850.083.498144
ATTCGGA6600.083.4520652
GTTCGGA7050.080.819312
TCGGAGA10650.080.67674
AGAGCGG29300.079.312248
AAGCGGC9150.078.2592549
ATCCGGA7650.077.58392
CGGAGAG18500.076.978365
TCGGGAG39050.075.8589253
TTGGGAG22550.071.787853
TCGGGAA12300.070.244023
CGGAAAG20050.068.659835
CCGGAGA9100.068.3359154
AAGGGGC26950.068.097639
GTCCGGA4000.066.4738852
TCGGAAA14650.066.425584
GATCCGG9600.065.297671
GGATCGG16750.064.925551
Produced by FastQC (version 0.11.2)