FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n02_30245-1.3420000003577e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n02_30245-1.3420000003577e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2289338
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG28400.12405332895361017No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC27930.1220003337209272No Hit
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC26720.1167149630155093No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG24240.10588213710688418No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA23540.10282448463267548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA5900.065.995952
AAGCGGC2350.060.551389
AGAGCGG6100.059.1032528
GAGCGGC6050.055.6638959
CGGGAGA6750.054.8582154
CGGAAAG5000.054.119745
GTTCGGA1950.053.5726972
GAGAGCG8350.049.9957777
TTGTGTT295400.049.9841421
GGTCCGG1800.047.492171
GGTTCGG2850.046.658971
GATCCGG2250.044.3260271
TCGGAGA3000.044.3085564
TCGGGAG8050.043.0523453
TTCGGAG2450.042.6310923
AAAGCGG3600.042.1672368
ATTCGGA2050.041.694052
AGCGGCG5650.040.71633510-11
GATCGGG10000.040.3683471
ATCGGAG2500.039.8794483
Produced by FastQC (version 0.11.2)