##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n02_30230-1.34200000035711.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2276208 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.785531902181173 33.0 33.0 33.0 27.0 33.0 2 31.613891612717293 33.0 33.0 33.0 27.0 33.0 3 31.547559801213247 33.0 33.0 33.0 27.0 33.0 4 35.22117486626881 37.0 37.0 37.0 33.0 37.0 5 35.258790057850604 37.0 37.0 37.0 33.0 37.0 6 35.42135560546312 37.0 37.0 37.0 33.0 37.0 7 35.28573662863851 37.0 37.0 37.0 33.0 37.0 8 35.224565153975384 37.0 37.0 37.0 33.0 37.0 9 35.043091843979106 37.0 37.0 37.0 33.0 37.0 10-11 35.01737692688893 37.0 37.0 37.0 33.0 37.0 12-13 34.97198344790986 37.0 37.0 37.0 33.0 37.0 14-15 36.6528416998798 40.0 37.0 40.0 33.0 40.0 16-17 36.555906797621304 40.0 37.0 40.0 33.0 40.0 18-19 36.62473201043139 40.0 37.0 40.0 33.0 40.0 20-21 36.59779642282252 40.0 37.0 40.0 33.0 40.0 22-23 36.762538177530345 40.0 37.0 40.0 33.0 40.0 24-25 36.40911946535642 40.0 37.0 40.0 33.0 40.0 26-27 36.24332925637728 40.0 37.0 40.0 33.0 40.0 28-29 36.19950110007521 40.0 37.0 40.0 33.0 40.0 30-31 36.22525555660994 40.0 37.0 40.0 33.0 40.0 32-33 36.17819461138876 40.0 37.0 40.0 33.0 40.0 34-35 36.070310138616506 40.0 37.0 40.0 33.0 40.0 36-37 35.90607624610756 38.5 37.0 40.0 27.0 40.0 38-39 35.862717510877744 37.0 37.0 40.0 27.0 40.0 40-41 35.71322568060564 37.0 37.0 40.0 27.0 40.0 42-43 35.59123463233588 37.0 37.0 40.0 27.0 40.0 44-45 35.45299111504748 37.0 37.0 40.0 27.0 40.0 46-47 35.30733636820537 37.0 37.0 40.0 27.0 40.0 48-49 35.18291518174086 37.0 37.0 40.0 27.0 40.0 50-51 34.02047396371509 37.0 35.0 38.5 24.5 38.5 52-53 34.46045440486985 37.0 33.0 38.5 27.0 40.0 54-55 35.17766610081328 37.0 37.0 40.0 27.0 40.0 56-57 35.2266908384471 37.0 37.0 40.0 27.0 40.0 58-59 34.979485398522456 37.0 35.0 40.0 27.0 40.0 60-61 34.76456127910981 37.0 33.0 40.0 27.0 40.0 62-63 34.5127690439538 37.0 33.0 40.0 27.0 40.0 64-65 34.26239341923058 37.0 33.0 37.0 27.0 40.0 66-67 33.92717097910209 37.0 33.0 37.0 27.0 40.0 68-69 33.75481612400976 37.0 33.0 37.0 27.0 40.0 70-71 33.45765347455065 37.0 33.0 37.0 27.0 37.0 72-73 33.2932891018747 37.0 33.0 37.0 27.0 37.0 74-75 33.14654174838152 37.0 33.0 37.0 27.0 37.0 76-77 32.87134919128656 37.0 33.0 37.0 22.0 37.0 78-79 32.68741828514793 37.0 33.0 37.0 22.0 37.0 80-81 32.49039652790958 37.0 33.0 37.0 22.0 37.0 82-83 32.14831992506836 35.0 33.0 37.0 22.0 37.0 84-85 31.928435582336938 33.0 33.0 37.0 22.0 37.0 86-87 31.829438258718007 33.0 33.0 37.0 22.0 37.0 88-89 31.7992312213998 33.0 33.0 37.0 22.0 37.0 90-91 31.7136364515018 33.0 33.0 37.0 18.5 37.0 92-93 31.521989422759255 33.0 33.0 37.0 15.0 37.0 94-95 31.29664314509043 33.0 33.0 37.0 15.0 37.0 96-97 31.143348498906953 33.0 33.0 37.0 15.0 37.0 98-99 31.007002215966203 33.0 33.0 37.0 15.0 37.0 100-101 29.59830494401215 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 -0.09200116176867823 2306 2 0.1043936037499762 2306 3 0.007124731690357322 2306 4 0.051214423702923284 2306 5 -0.041416872023525286 2306 6 -0.039808179193293824 2306 7 -0.03402612302512864 2306 8 -0.06035858176193187 2306 9 0.07854585416988868 2306 10-11 0.14473749659164525 2306 12-13 0.13137256588922952 2306 14-15 0.30041263076822133 2306 16-17 0.2615871529798568 2306 18-19 0.22103899146686246 2306 20-21 0.3210613641409168 2306 22-23 0.2691159196071595 2306 24-25 0.28792281732054903 2306 26-27 0.039812390060497194 2306 28-29 -0.023710788907258973 2306 30-31 -0.0014262936546529659 2306 32-33 0.09841018772798549 2306 34-35 0.027954670174523244 2306 36-37 0.042591292704166506 2306 38-39 -0.1413824930887415 2306 40-41 -0.010193999942373466 2306 42-43 -0.003034724255570609 2306 44-45 0.10728440913984372 2306 46-47 0.12718033421156605 2306 48-49 -0.05990617812442878 2306 50-51 0.13342252780380903 2306 52-53 -0.027698221189652372 2306 54-55 0.12239308990044862 2306 56-57 0.10133733288554225 2306 58-59 0.17934654502659697 2306 60-61 0.09885022822465572 2306 62-63 0.21801296378664858 2306 64-65 0.11575797613790684 2306 66-67 0.21784860798218375 2306 68-69 -0.1839169411214172 2306 70-71 0.16804708960906822 2306 72-73 -0.009517185823881391 2306 74-75 0.050657664278169534 2306 76-77 0.04596540106394542 2306 78-79 0.03236810619198849 2306 80-81 0.09174202004992082 2306 82-83 0.07069492599269722 2306 84-85 0.0583996494558896 2306 86-87 0.20757074915032092 2306 88-89 0.13986607623291647 2306 90-91 -0.01518945084060519 2306 92-93 0.10338717793342411 2306 94-95 -0.08261930355462255 2306 96-97 -0.13368290363555602 2306 98-99 0.022986889505840935 2306 100-101 -0.019124656480581592 2307 1 -0.05752071359966138 2307 2 -0.024674301439006285 2307 3 -0.04867642926089033 2307 4 0.019240218261622033 2307 5 0.001824312983465859 2307 6 -0.013957076420346937 2307 7 0.19624456949275526 2307 8 0.13946296120502666 2307 9 0.2635325842879439 2307 10-11 0.2113523039495675 2307 12-13 0.1474505313483121 2307 14-15 0.32823329779223087 2307 16-17 0.34282367064906794 2307 18-19 0.35895261303335957 2307 20-21 0.38289296737798395 2307 22-23 0.2909476535819735 2307 24-25 0.31253893859289406 2307 26-27 0.23156528917017027 2307 28-29 0.192247061241126 2307 30-31 0.20988121461451925 2307 32-33 0.2158403083989313 2307 34-35 0.09683434568341198 2307 36-37 0.16296203228763773 2307 38-39 0.10184185943253965 2307 40-41 0.003684956508877235 2307 42-43 0.09889414786983508 2307 44-45 0.17569865824756192 2307 46-47 -0.030643339780773715 2307 48-49 -0.04761356088534541 2307 50-51 -0.05057958361090442 2307 52-53 -0.013397962328902224 2307 54-55 -0.041641117632657654 2307 56-57 -0.10108899639056546 2307 58-59 -0.009152781673975596 2307 60-61 0.08130473148656137 2307 62-63 0.023545622077378425 2307 64-65 0.0359911142361895 2307 66-67 0.05789815759062833 2307 68-69 0.08974773615243947 2307 70-71 0.10657583230409529 2307 72-73 -0.04958536556215165 2307 74-75 0.015853640170128358 2307 76-77 -0.014661126933546598 2307 78-79 -0.02765375969722328 2307 80-81 -0.003522495109116619 2307 82-83 -0.08667214639680054 2307 84-85 -0.10286144691091081 2307 86-87 -0.02053950199690746 2307 88-89 -0.20382746139994268 2307 90-91 -0.20061072056566331 2307 92-93 -0.016515357071611447 2307 94-95 -0.1386513800623561 2307 96-97 -0.1425614678735485 2307 98-99 -0.18915766722780702 2307 100-101 -0.2028316274175772 2201 1 0.13672885719529404 2201 2 0.11413067170659374 2201 3 0.10953399999313618 2201 4 0.27602331113399714 2201 5 0.14791107730398068 2201 6 0.28958916139231405 2201 7 0.32318058426573515 2201 8 0.2882342469588508 2201 9 0.3295205640975567 2201 10-11 0.29959098358994396 2201 12-13 0.26435793449246603 2201 14-15 0.3920020854624795 2201 16-17 0.32866786370684054 2201 18-19 0.32589494648833295 2201 20-21 0.35776166492128425 2201 22-23 0.35549816385228183 2201 24-25 0.4165937629555714 2201 26-27 0.28939179114292557 2201 28-29 0.19805510556007278 2201 30-31 0.29177549669713443 2201 32-33 0.34272871876058986 2201 34-35 0.09840192374240786 2201 36-37 0.2088424291890334 2201 38-39 0.22604023788240823 2201 40-41 0.3155934984852564 2201 42-43 0.24187040015668515 2201 44-45 0.18800309748902322 2201 46-47 0.3053583917340177 2201 48-49 0.2373130762778075 2201 50-51 0.1827767814815573 2201 52-53 0.276442644019653 2201 54-55 0.37704212904329637 2201 56-57 0.3530655064050876 2201 58-59 0.43548435944080666 2201 60-61 0.4971363055486222 2201 62-63 0.4300151993379586 2201 64-65 0.5198630152043222 2201 66-67 0.51262467866637 2201 68-69 0.32441590126106945 2201 70-71 0.41257484531617905 2201 72-73 0.3884765501740617 2201 74-75 0.33481391084482937 2201 76-77 0.3053643498452985 2201 78-79 0.39489539377083105 2201 80-81 0.43851997598535775 2201 82-83 0.3927437673752294 2201 84-85 0.3919109458368091 2201 86-87 0.4698211404514083 2201 88-89 0.43539385900865923 2201 90-91 0.26304752078349836 2201 92-93 0.43835413030694426 2201 94-95 0.39151772427671716 2201 96-97 0.32583976475926235 2201 98-99 0.3872644035855153 2201 100-101 0.2798166411900844 1101 1 0.14335241657611064 1101 2 0.07999318664882793 1101 3 0.13519819256584142 1101 4 0.23843750897457028 1101 5 0.16995126230006719 1101 6 0.14995794623497716 1101 7 0.2598109947208229 1101 8 0.14361877560436653 1101 9 0.14876781156598895 1101 10-11 0.13238832334570105 1101 12-13 0.17575127670619395 1101 14-15 0.285540634002885 1101 16-17 0.2661848418990118 1101 18-19 0.1971780393696534 1101 20-21 0.31342160333565516 1101 22-23 0.3440196245719349 1101 24-25 0.30267457829606315 1101 26-27 0.16394679877705443 1101 28-29 0.17087647248322213 1101 30-31 0.2798520362383101 1101 32-33 0.11234796304665196 1101 34-35 0.07239190283609531 1101 36-37 0.21892612277801504 1101 38-39 0.1742141105162105 1101 40-41 0.023841240848412326 1101 42-43 0.1703814618226147 1101 44-45 0.24995534612777703 1101 46-47 0.12499161722320196 1101 48-49 0.22319590525324173 1101 50-51 0.13082522193672474 1101 52-53 0.15630509859498432 1101 54-55 0.3859275965080968 1101 56-57 0.39153545593038075 1101 58-59 0.4079594709060146 1101 60-61 0.3893654332639258 1101 62-63 0.42724558279878977 1101 64-65 0.3807363762570759 1101 66-67 0.3761704142855251 1101 68-69 0.45168030090365363 1101 70-71 0.38814774718179734 1101 72-73 0.10298095211742009 1101 74-75 0.14509596097327915 1101 76-77 0.12511247177960882 1101 78-79 0.34037283050090394 1101 80-81 0.3791055577486233 1101 82-83 0.3177230803201141 1101 84-85 0.3537742956647314 1101 86-87 0.4505013103215987 1101 88-89 0.31371638799474155 1101 90-91 0.17513422816175606 1101 92-93 0.22431671763251515 1101 94-95 0.30039872117351507 1101 96-97 0.2235514603916009 1101 98-99 0.41029722552763204 1101 100-101 0.1634262575242289 2304 1 -0.04265356594842373 2304 2 -0.11698804643410199 2304 3 -0.12795306034466947 2304 4 -0.16682926240647333 2304 5 -0.15030981056037973 2304 6 -0.10887212614650821 2304 7 0.04264098068924227 2304 8 -0.14612030433878687 2304 9 -0.12106227609585574 2304 10-11 -0.09465675529809658 2304 12-13 -0.11701568567935539 2304 14-15 -0.059988490627425506 2304 16-17 -0.04951675584707971 2304 18-19 -0.010888963429813714 2304 20-21 -0.027092796823261267 2304 22-23 -0.029389966541423007 2304 24-25 -0.005252609206081615 2304 26-27 -0.1586042205020135 2304 28-29 -0.0697890715160483 2304 30-31 -0.07287018630591291 2304 32-33 -0.1263390377400171 2304 34-35 -0.1606329340724031 2304 36-37 0.0049074196169272 2304 38-39 -0.04669748416854702 2304 40-41 -0.09658183017734245 2304 42-43 -0.029496980276149998 2304 44-45 0.002773782251388468 2304 46-47 -0.17262093185177463 2304 48-49 -0.06681914326717475 2304 50-51 -0.023801355035978133 2304 52-53 0.07093583287952754 2304 54-55 0.06759909405191422 2304 56-57 0.14577536074953912 2304 58-59 0.05420898549112252 2304 60-61 0.06455529174143493 2304 62-63 0.015927656986519878 2304 64-65 0.1163674366982761 2304 66-67 0.2487943841125997 2304 68-69 0.12236785389094251 2304 70-71 -0.018773123208603693 2304 72-73 0.10454165651042047 2304 74-75 0.06725688319044565 2304 76-77 0.06627913256246387 2304 78-79 0.17671081837250568 2304 80-81 0.08681492107924527 2304 82-83 0.03234944126014483 2304 84-85 0.14103466404787568 2304 86-87 0.22579504890414626 2304 88-89 0.2351571573661566 2304 90-91 0.06494201703565139 2304 92-93 -0.013409596359924336 2304 94-95 0.08801830975244584 2304 96-97 -0.09365090585123781 2304 98-99 0.21243262954299524 2304 100-101 0.29192341827519996 2202 1 -0.0716656710579393 2202 2 -0.17365415494294467 2202 3 -0.06605311087524157 2202 4 -0.12568710356068635 2202 5 -0.053571428022713974 2202 6 0.004790540059801174 2202 7 -0.09164462916239557 2202 8 -0.09009904099548294 2202 9 -0.017099207373789227 2202 10-11 -0.05468796274164589 2202 12-13 -0.08274899392029056 2202 14-15 -0.1153068702705653 2202 16-17 -0.14113935027330626 2202 18-19 -0.03611498030198845 2202 20-21 -0.06370276354618909 2202 22-23 -0.0012332506432599644 2202 24-25 -0.21733368829803368 2202 26-27 -0.06962805976836961 2202 28-29 -0.1761859306187361 2202 30-31 -0.0641720622595372 2202 32-33 -0.12674135592257585 2202 34-35 -0.1717228631764769 2202 36-37 -0.10175962738737354 2202 38-39 -0.18398627947132695 2202 40-41 -0.2324333690876017 2202 42-43 -0.18374500585458975 2202 44-45 -0.15201696536890807 2202 46-47 -0.10320919068092138 2202 48-49 -0.09895841401668548 2202 50-51 -0.41301295464220544 2202 52-53 -0.20919863870060595 2202 54-55 0.006611460952264281 2202 56-57 -2.965171354105678E-4 2202 58-59 -0.07945894616862148 2202 60-61 -0.12249142112883504 2202 62-63 -0.04435647498173978 2202 64-65 0.0029634741728941094 2202 66-67 0.12632764266266605 2202 68-69 -0.08100077675034356 2202 70-71 -0.03921503602040133 2202 72-73 -0.02303185855515011 2202 74-75 0.007505841337014374 2202 76-77 -0.043999516616572976 2202 78-79 0.006498244212842508 2202 80-81 -0.034329900129968394 2202 82-83 0.09018941000331182 2202 84-85 0.08645139801454249 2202 86-87 0.09577243116371648 2202 88-89 -0.08233424922613253 2202 90-91 -0.0944923072994861 2202 92-93 -0.13547710678339797 2202 94-95 -0.01358864143640659 2202 96-97 0.161273478582185 2202 98-99 -0.037581694861295034 2202 100-101 0.14705182668076233 1102 1 -0.03438959386703999 1102 2 0.06706080546211979 1102 3 0.017280222458090577 1102 4 -0.025277425042993684 1102 5 -0.00623735880571985 1102 6 -0.1484778268276301 1102 7 0.08571569023308001 1102 8 0.19597024190236567 1102 9 0.033594390438331345 1102 10-11 0.11929703950993797 1102 12-13 0.16653297033992942 1102 14-15 0.16401452417405693 1102 16-17 0.17116569547229687 1102 18-19 0.16106271363630498 1102 20-21 0.1784949475261186 1102 22-23 0.07886480310919808 1102 24-25 0.060233804315672046 1102 26-27 0.027733055919135552 1102 28-29 0.15654057327485305 1102 30-31 0.11044448194291334 1102 32-33 0.045877593630955005 1102 34-35 0.08027747730700696 1102 36-37 0.21299058404188997 1102 38-39 0.14704810386914602 1102 40-41 0.07211469400068893 1102 42-43 -0.03767141199647028 1102 44-45 0.21681108497871548 1102 46-47 0.14927784897991359 1102 48-49 0.29349067821039654 1102 50-51 0.27365917623524894 1102 52-53 0.2626922528169615 1102 54-55 0.19930314766352808 1102 56-57 0.43075145808617776 1102 58-59 0.4433232432500276 1102 60-61 0.4330602444944063 1102 62-63 0.4776108880285648 1102 64-65 0.404758014355437 1102 66-67 0.4756376144354846 1102 68-69 0.4483714156338081 1102 70-71 0.3760896967000633 1102 72-73 0.32521204294627637 1102 74-75 0.45205096638231623 1102 76-77 0.3212198206487855 1102 78-79 0.4583012964825528 1102 80-81 0.4320418639039971 1102 82-83 0.5525501961906585 1102 84-85 0.4632169696423851 1102 86-87 0.4132382729816726 1102 88-89 0.38578935726282637 1102 90-91 0.20351311846962616 1102 92-93 0.35170465099006165 1102 94-95 0.44615467794808694 1102 96-97 0.21164718666701887 1102 98-99 0.3697807113684526 1102 100-101 0.318431031926405 2305 1 0.2788479865159701 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-0.024914561409339342 1213 60-61 -0.055091215462446996 1213 62-63 -0.0050314699336908575 1213 64-65 -0.04188112601458016 1213 66-67 -0.07367723823670502 1213 68-69 0.08092673721370858 1213 70-71 0.051725145549056606 1213 72-73 0.11509475197448893 1213 74-75 -0.0046332228408019205 1213 76-77 0.09925655141112344 1213 78-79 0.10478468079525527 1213 80-81 0.2123464079844979 1213 82-83 0.11365466802904933 1213 84-85 0.06212462781932615 1213 86-87 0.17979728095905045 1213 88-89 0.009369492217249586 1213 90-91 0.14770544133553543 1213 92-93 0.1502903578100252 1213 94-95 0.17885435567650987 1213 96-97 0.2045485018834654 1213 98-99 0.02255934307198615 1213 100-101 0.2319162914600561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1074.0 3 257.0 4 265.0 5 260.0 6 708.0 7 3645.0 8 15655.0 9 21950.0 10 20167.0 11 14317.0 12 8997.0 13 6188.0 14 5121.0 15 5237.0 16 5224.0 17 5218.0 18 5632.0 19 6042.0 20 6733.0 21 7554.0 22 8695.0 23 9666.0 24 10855.0 25 12810.0 26 15442.0 27 18690.0 28 23332.0 29 28286.0 30 35888.0 31 47379.0 32 66488.0 33 96831.0 34 150028.0 35 263232.0 36 500206.0 37 695609.0 38 152393.0 39 134.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.04603874251838 20.852712597597684 27.022512187179565 12.078736472704374 2 36.81659107008807 18.993684425695083 33.23134843855179 10.958376065665055 3 43.45702729317838 15.426908434936253 27.067863881152327 14.048200390733037 4 35.871504689770575 13.81240917697829 35.02558456999428 15.290501563256859 5 34.646943287210334 12.78226481235192 36.906559095272904 15.664232805164835 6 27.2542851521563 13.362614784594712 44.00422012057362 15.378879942675376 7 37.80242321364223 13.834502172902019 31.348880021577223 17.014194591878535 8 33.892251293973544 26.54159282379257 23.180825432805772 16.385330449428118 9 34.72453957963369 27.769681384311728 20.39785281592467 17.107926220129915 10-11 31.11673048385834 26.525059353066965 26.166324050592827 16.191886112481868 12-13 31.64966501500223 22.819518102871264 26.31300063809808 19.217816244028416 14-15 29.39977018128579 23.429256837370705 26.320357785152936 20.85061519619057 16-17 27.798613128844586 26.13203187599761 27.629383790979112 18.439971204178693 18-19 27.131639060566425 26.371510073512418 27.063969899208796 19.43288096671236 20-21 25.9235612335542 27.70730986364343 27.3657751369141 19.00335376588827 22-23 27.491733491733495 25.246873246873246 27.312279312279315 19.94911394911395 24-25 27.685233812858247 25.11727043088308 27.68631642811203 19.51117932814665 26-27 23.858245214176446 26.025285141467684 29.46957214028493 20.64689750407093 28-29 23.390782447112574 27.140540596461488 27.284737512527258 22.183939443898684 30-31 22.916584037772203 27.400292263908316 27.809602755530406 21.873520942789078 32-33 22.580158833192527 24.923731987116486 29.99338103192864 22.502728147762348 34-35 22.46334306971909 26.595608905453457 28.49496461109234 22.446083413735117 36-37 21.013140114123292 29.712104170510045 28.43232225183782 20.842433463528838 38-39 20.490547397252666 28.12448820031005 27.782101784848724 23.602862617588563 40-41 22.546607605567672 27.60728704333364 26.423148012419144 23.422957338679545 42-43 25.021196688069846 27.10294734270911 26.758068519864924 21.11778744935612 44-45 21.752576706061433 29.150694847160956 26.49085928113422 22.60586916564339 46-47 22.311675416516206 29.28805457049019 27.183174539767613 21.21709547322599 48-49 22.24366184080521 27.158135324873783 28.604549039802446 21.99365379451856 50-51 20.718045629118297 27.26666868018502 27.216756940630827 24.798528750065863 52-53 22.534999224100837 28.35210480281581 26.652025978514647 22.460869994568704 54-55 23.234004586302632 27.96769574880956 27.514333297773764 21.28396636711404 56-57 24.787386794020776 27.46954685866771 25.096073262999376 22.646993084312143 58-59 22.983830262945023 26.132280536408025 26.036732691292958 24.84715650935399 60-61 20.81298197327669 26.054493348920282 28.538040562055627 24.59448411574741 62-63 22.24476719821301 26.058645629846506 28.49691820044234 23.19966897149815 64-65 23.033799226635335 28.221970819454516 27.16449997047217 21.57972998343797 66-67 22.242231074994777 27.749901045436232 26.87497961403753 23.13288826553146 68-69 21.329303910969568 24.75699401095736 30.6477324362477 23.265969641825368 70-71 21.141890613119717 28.89579580022874 28.39927372409844 21.5630398625531 72-73 23.53609510945179 29.776540288380172 26.5431194382652 20.14424516390284 74-75 24.746954264734327 28.04634108622082 26.928503041659745 20.278201607385107 76-77 23.30037306116802 27.2963838137188 26.663232876615755 22.740010248497423 78-79 23.684488828119115 28.722906420763522 26.40004882341303 21.192555927704333 80-81 22.688382386575714 29.651207627706842 26.26990733742846 21.39050264828898 82-83 22.449781096380402 29.30141480169745 26.901243778079227 21.347560323842917 84-85 22.87327349192511 28.414318744969997 26.842622326551446 21.869785436553446 86-87 20.554213580396823 29.192760253989213 27.959484960683596 22.293541204930367 88-89 20.04186927652852 29.441673924771944 28.45455691471168 22.061899883987856 90-91 21.55093781699572 28.57825704941183 27.04722350590682 22.823581627685627 92-93 21.972532956706246 28.755323860368886 25.991477416696597 23.280665766228275 94-95 22.63345009119275 30.006160302709695 25.714725106406227 21.645664499691325 96-97 21.271479094456343 31.33106755702288 24.6385654872598 22.758887861260977 98-99 21.552371035189502 29.169347063133944 26.464620419497198 22.81366148217936 100-101 23.10250792168976 29.840539217047468 26.218754559186447 20.83819830207632 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 701.0 1 548.5 2 297.0 3 235.0 4 267.5 5 275.0 6 330.0 7 445.0 8 566.5 9 708.5 10 830.5 11 947.5 12 980.5 13 863.0 14 734.0 15 649.0 16 612.0 17 545.5 18 516.5 19 549.0 20 564.5 21 627.0 22 739.5 23 853.5 24 988.5 25 1174.0 26 1474.0 27 1928.0 28 2419.0 29 2968.0 30 3717.0 31 4832.5 32 6641.0 33 9500.5 34 14292.5 35 23808.0 36 36215.5 37 49590.0 38 66140.0 39 80522.5 40 90492.0 41 97653.5 42 133463.5 43 172119.5 44 162216.5 45 139936.5 46 137318.5 47 133291.5 48 118252.5 49 107472.0 50 97123.5 51 91537.5 52 89875.0 53 90897.5 54 78516.5 55 49869.0 56 32875.5 57 26638.0 58 22746.5 59 18874.0 60 14738.0 61 11584.0 62 8905.0 63 6727.5 64 5257.0 65 4070.5 66 3190.5 67 2557.5 68 1994.0 69 1432.5 70 1066.5 71 863.5 72 626.5 73 420.5 74 306.5 75 222.5 76 168.0 77 125.0 78 93.5 79 77.5 80 66.5 81 54.0 82 50.0 83 35.5 84 26.5 85 28.5 86 23.0 87 18.5 88 16.0 89 10.5 90 6.0 91 6.0 92 5.0 93 4.0 94 5.0 95 5.0 96 3.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2994014606749471 2 0.27519453406718547 3 0.24619894139727125 4 0.23319485741197643 5 0.24022409199862227 6 0.1860550529652826 7 0.15117247632905254 8 0.1477896571842292 9 0.1276245404637889 10-11 0.11097404103667151 12-13 0.13384101980135382 14-15 0.13671861271026198 16-17 0.1301067389271982 18-19 0.11626793333473918 20-21 0.12272604261122007 22-23 0.15301765040804705 24-25 0.5785719055552041 26-27 0.22976810555098656 28-29 0.20479235641031046 30-31 0.30867126378608634 32-33 0.33949006417691174 34-35 0.347617616667721 36-37 0.33812814997574914 38-39 0.3455088462917273 40-41 0.34862806913955136 42-43 0.35871062749977156 44-45 0.34089591109424094 46-47 0.3588863583644377 48-49 0.3550202793417825 50-51 0.36044596978834975 52-53 0.3458383416629763 54-55 0.3388969725086635 56-57 0.34649733240547437 58-59 0.3387651743601639 60-61 0.316996513499645 62-63 0.30388259772393383 64-65 0.3146461131847353 66-67 0.32905604408735933 68-69 0.31767747060022633 70-71 0.2997309560461961 72-73 0.2960406078882071 74-75 0.2881546853363137 76-77 0.2902634557123075 78-79 0.2989621335132817 80-81 0.3078365421789221 82-83 0.33017632834960603 84-85 0.3216753477713812 86-87 0.32385001722162476 88-89 0.32861671692569394 90-91 0.3228395647497944 92-93 0.3050248483442638 94-95 0.300455845862944 96-97 0.31756763880981004 98-99 0.326002720313785 100-101 0.32672761013053286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2276208.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.72830311024367 #Duplication Level Percentage of deduplicated Percentage of total 1 88.41481722337254 41.314743786505446 2 5.08543120230242 4.752671413349567 3 1.8496763440042858 2.5929671057543886 4 0.9645946717253711 1.8029548879563655 5 0.6868584666345664 1.6047865311368605 6 0.40264163501816463 1.128885621955974 7 0.2610111947697828 0.8537627157058479 8 0.2603281206427465 0.9731753063611475 9 0.21149841617313703 0.889466588844733 >10 1.5175548363629765 13.844340760005386 >50 0.18876874523880072 6.165559727077438 >100 0.14254555096137606 13.001672908956612 >500 0.009081863365391457 2.873743480096248 >1k 0.004439363705186883 3.387999243602903 >5k 2.8213714621282967E-4 0.7821942658540437 >10k+ 4.702285770060685E-4 4.031075656836889 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 33348 1.4650682187216635 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 22778 1.0006994088413712 No Hit TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 12776 0.5612843817436719 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 12023 0.5282030464702698 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 10803 0.47460513274709515 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 6140 0.2697468772625349 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 6110 0.2684288957775388 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 5549 0.24378264200811173 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 3344 0.14691100286089848 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3160 0.13882738308625572 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3007 0.13210567751277563 No Hit AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 2763 0.1213860947681407 No Hit TGTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAA 2562 0.11255561881866682 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 2515 0.11049078115883962 No Hit AACTTTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAA 2409 0.10583391324518673 No Hit GTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCCT 2375 0.10434020089552448 No Hit GTGTTTGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTCAAC 2344 0.10297828669436186 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 2314 0.10166030520936575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0027677611184918076 0.0 0.0 2 0.003031357415491027 0.0 0.0 3 0.0033388864286567834 0.0 0.0 4 0.0034706845771563933 0.0 0.0 5 0.0038221463064886867 0.0 0.0 6 0.004129675319654443 0.0 0.0 7 0.004744733345985955 0.0 0.0 8 0.005579454953150152 0.0 0.0 9 0.006370243844147811 0.0 0.0 10-11 0.009467500333888643 0.0 0.0 12-13 0.012850319478711961 0.0 0.0 14-15 0.016584600352867577 0.0 0.0 16-17 0.02095590561143797 0.0 0.0 18-19 0.02563473988317412 0.0 0.0 20-21 0.029149357176497054 0.0 0.0 22-23 0.03266397446981998 0.0 0.0 24-25 0.03668381799905808 0.0 0.0 26-27 0.041208887764211354 0.0 0.0 28-29 0.04634901555569614 0.0 0.0 30-31 0.09772832711246073 0.0 0.0 32-33 0.10543851879968791 0.0 0.0 34-35 0.11150123363066997 0.0 0.0 36-37 0.11929929075023021 0.0 0.0 38-39 0.12795403583503792 0.0 0.0 40-41 0.13735563709467677 0.0 0.0 42-43 0.14781162354231248 0.0 0.0 44-45 0.16053014487252482 0.0 0.0 46-47 0.173314565276987 0.0 0.0 48-49 0.18989916562985457 0.0 0.0 50-51 0.2097787196952124 0.0 0.0 52-53 0.23040512993540135 0.0 0.0 54-55 0.25582020623774276 0.0 0.0 56-57 0.2847718661914904 0.0 0.0 58-59 0.31499757491406766 0.0 0.0 60-61 0.3488038000042175 0.0 0.0 62-63 0.3859928442391908 0.0 0.0 64-65 0.42950819960214537 0.0 0.0 66-67 0.47871284170866635 0.0 0.0 68-69 0.5319373273444254 0.0 0.0 70-71 0.5894232864483386 0.0 0.0 72-73 0.6522690369245693 0.0 0.0 74-75 0.7224954837167781 0.0 0.0 76-77 0.8012009447291284 0.0 0.0 78-79 0.8877483955772056 0.0 4.393271616653663E-5 80-81 0.9900896578871526 0.0 4.393271616653663E-5 82-83 1.1032383683740679 0.0 4.393271616653663E-5 84-85 1.2309507742701897 0.0 4.393271616653663E-5 86-87 1.3733147410078517 0.0 4.393271616653663E-5 88-89 1.526595987712898 0.0 4.393271616653663E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCGGA 345 0.0 68.797295 2 GGTTCGG 395 0.0 64.91304 1 GAGCGGC 745 0.0 61.07941 9 AAGCGGC 440 0.0 58.17293 9 GGATCGG 750 0.0 56.979225 1 CGGGAGA 1130 0.0 56.259792 4 TCGGAAA 605 0.0 54.892677 4 AGAGCGG 845 0.0 54.414417 8 TCGGAGA 490 0.0 54.220524 4 TCGGGAA 420 0.0 53.097946 3 ACCGGAA 190 0.0 52.443886 3 TTGTGTT 30200 0.0 51.963673 1 GGTCCGG 225 0.0 50.6482 1 TCGGGAG 1200 0.0 49.03087 3 ATCGGGA 1510 0.0 47.784508 2 CGGAGAG 780 0.0 46.833683 5 CGGAAAG 910 0.0 45.356552 5 AAGGGGC 1265 0.0 44.58988 9 ATCCGGA 285 0.0 43.306087 2 ATTCGGA 255 0.0 42.8162 2 >>END_MODULE