FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_53115004-1.3410000003588d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_53115004-1.3410000003588d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4822136
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT54450711.291821715521918TruSeq Adapter, Index 13 (97% over 38bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC89190.18495952830861676No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT72260.1498506056237319TruSeq Adapter, Index 13 (97% over 38bp)
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG71790.14887593381854017No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA67730.1404564284375223No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC63680.13205766075448722No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG50470.10466316171920494No Hit
GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT49320.10227832645118262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA677850.086.8145751
TCGGAAG678150.086.300173
CGGAAGA679450.085.8763664
ATCGGAA683750.085.635042
GAAGAGC683500.085.2290346
GGAAGAG707400.082.544215
TCGGACG3000.072.838883
GAGCACA864200.067.512479
AGAGCAC863200.067.486158
AAGAGCA870000.066.909547
ATCGGAC3200.066.801962
GCGCGTC301.2877234E-463.4711341
CGAGCAC4000.052.241528
GATCGGC2550.046.669951
CGGACGA5100.046.5721744
TTGTGTT424750.046.521841
GCCGTCT634500.046.0108950-51
CGTCTTC634550.045.9757252-53
ATGCCGT634600.045.9500548-49
TCGTATG639250.045.8300144-45
Produced by FastQC (version 0.11.2)