##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_53115004-1.3410000003588d.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4822136 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.669019082000176 33.0 33.0 33.0 33.0 33.0 2 32.68530170032533 33.0 33.0 33.0 33.0 33.0 3 32.72673956105759 33.0 33.0 33.0 33.0 33.0 4 36.642043484464146 37.0 37.0 37.0 37.0 37.0 5 36.643807018300606 37.0 37.0 37.0 37.0 37.0 6 36.515019692518 37.0 37.0 37.0 37.0 37.0 7 36.35658305779845 37.0 37.0 37.0 37.0 37.0 8 36.306488245043276 37.0 37.0 37.0 37.0 37.0 9 36.366035507915996 37.0 37.0 37.0 37.0 37.0 10-11 36.29479788624792 37.0 37.0 37.0 35.0 37.0 12-13 36.30489890786987 37.0 37.0 37.0 35.0 37.0 14-15 38.12282015687654 40.0 37.0 40.0 35.0 40.0 16-17 38.160929720771044 40.0 37.0 40.0 37.0 40.0 18-19 38.114102754464 40.0 37.0 40.0 37.0 40.0 20-21 38.12874025120818 40.0 37.0 40.0 37.0 40.0 22-23 37.834823198682074 40.0 37.0 40.0 33.0 40.0 24-25 38.00188319035382 40.0 37.0 40.0 35.0 40.0 26-27 37.975542788507 40.0 37.0 40.0 35.0 40.0 28-29 37.748005551896505 40.0 37.0 40.0 33.0 40.0 30-31 37.70262949862882 40.0 37.0 40.0 33.0 40.0 32-33 37.53963762117037 40.0 37.0 40.0 33.0 40.0 34-35 37.45672094275234 40.0 37.0 40.0 33.0 40.0 36-37 37.51032250023641 40.0 37.0 40.0 33.0 40.0 38-39 37.32998696013551 40.0 37.0 40.0 33.0 40.0 40-41 37.022121317192216 38.5 37.0 40.0 33.0 40.0 42-43 36.829008348167704 37.0 37.0 40.0 33.0 40.0 44-45 36.7333703362991 37.0 37.0 40.0 33.0 40.0 46-47 36.96113382119459 37.0 37.0 40.0 33.0 40.0 48-49 36.632110230818874 37.0 37.0 40.0 33.0 40.0 50-51 36.6116765474885 37.0 37.0 40.0 33.0 40.0 52-53 36.52787468043208 37.0 37.0 40.0 33.0 40.0 54-55 36.21568709385218 37.0 37.0 40.0 33.0 40.0 56-57 36.13590294840294 37.0 37.0 40.0 33.0 40.0 58-59 35.99783944293566 37.0 37.0 40.0 33.0 40.0 60-61 35.70851350936597 37.0 35.0 40.0 27.0 40.0 62-63 35.48917896550408 37.0 33.0 40.0 27.0 40.0 64-65 35.14700891886915 37.0 33.0 38.5 27.0 40.0 66-67 34.28794646189987 37.0 33.0 37.0 27.0 40.0 68-69 34.121243262321926 37.0 33.0 37.0 27.0 40.0 70-71 33.54264997917935 37.0 33.0 37.0 27.0 38.5 72-73 32.20828529099967 37.0 33.0 37.0 18.5 37.0 74-75 31.276941069268887 35.0 27.0 37.0 15.0 37.0 76-77 28.845085974348297 33.0 27.0 35.0 15.0 37.0 78-79 30.67177201140739 33.0 30.0 37.0 15.0 37.0 80-81 30.88101797626612 33.0 33.0 37.0 15.0 37.0 82-83 30.746481020029297 33.0 33.0 37.0 15.0 37.0 84-85 30.527949025079344 33.0 30.0 37.0 15.0 37.0 86-87 30.405854687632203 33.0 27.0 37.0 15.0 37.0 88-89 30.27622883303167 33.0 27.0 37.0 15.0 37.0 90-91 30.132968771515362 33.0 27.0 37.0 6.0 37.0 92-93 30.003110654697423 33.0 27.0 37.0 6.0 37.0 94-95 29.8761596520712 33.0 27.0 37.0 6.0 37.0 96-97 29.709813970406472 33.0 27.0 37.0 6.0 37.0 98-99 29.540438925820425 33.0 27.0 37.0 6.0 37.0 100-101 28.06452234030728 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.03983388735139215 2306 2 0.06320991763272588 2306 3 0.09723607372131227 2306 4 0.15325421128378025 2306 5 0.10813833028338138 2306 6 0.2077853059690824 2306 7 0.24447555307335733 2306 8 0.17539653105330189 2306 9 0.26377408359816457 2306 10-11 0.28707679922094087 2306 12-13 0.23089133978686505 2306 14-15 0.42118203758574424 2306 16-17 0.44201863612908454 2306 18-19 0.36014296261114254 2306 20-21 0.4804262937797503 2306 22-23 0.5562804371167189 2306 24-25 0.42712294045036714 2306 26-27 0.42909214625442615 2306 28-29 0.46193026287659933 2306 30-31 0.4435487836161087 2306 32-33 0.5214319146625073 2306 34-35 0.5916959641995874 2306 36-37 0.5675998350414417 2306 38-39 0.5887770348139725 2306 40-41 0.677422881407665 2306 42-43 0.5676510261546 2306 44-45 0.6629519800563131 2306 46-47 0.49654051959387147 2306 48-49 0.5858734878020186 2306 50-51 0.578025419097564 2306 52-53 0.6476255168792662 2306 54-55 0.6266499457407875 2306 56-57 0.6219851568647954 2306 58-59 0.622553633610238 2306 60-61 0.6497070054090557 2306 62-63 0.7051987986398274 2306 64-65 0.5499051939159685 2306 66-67 0.6519934940908527 2306 68-69 0.7460676562822215 2306 70-71 0.5582633264254682 2306 72-73 0.20251830186974473 2306 74-75 0.5216245780854933 2306 76-77 0.6437470653231152 2306 78-79 0.7853108968007056 2306 80-81 0.8949137194372234 2306 82-83 0.7482252407161916 2306 84-85 0.9446097514310985 2306 86-87 0.8084168286808939 2306 88-89 0.7492891750321355 2306 90-91 0.6457518441463392 2306 92-93 0.8583189434733463 2306 94-95 0.8148126112619067 2306 96-97 0.9455008311096726 2306 98-99 1.1425459306014965 2306 100-101 0.8411817361745086 2307 1 0.06956416492941031 2307 2 0.08669058334954372 2307 3 0.13290507517341155 2307 4 0.11563431467877194 2307 5 0.1320891445809096 2307 6 0.1996128815921807 2307 7 0.1810917215669221 2307 8 0.20058137935853182 2307 9 0.19558519978027533 2307 10-11 0.2122626275913646 2307 12-13 0.2272220322528682 2307 14-15 0.43564944895746294 2307 16-17 0.425165943979529 2307 18-19 0.4294371997618569 2307 20-21 0.43789179430705616 2307 22-23 0.4704495788406433 2307 24-25 0.3995627062562548 2307 26-27 0.44664313485277773 2307 28-29 0.4664031767489476 2307 30-31 0.5768688420920753 2307 32-33 0.5648279291331804 2307 34-35 0.49531875633181244 2307 36-37 0.5566065191802778 2307 38-39 0.49357967465726205 2307 40-41 0.525956397611111 2307 42-43 0.4914839876128738 2307 44-45 0.5652039060022176 2307 46-47 0.47567417777484167 2307 48-49 0.43570950122099816 2307 50-51 0.5156145850046059 2307 52-53 0.6533201197073666 2307 54-55 0.5144043044629214 2307 56-57 0.5396004056991046 2307 58-59 0.5238355306093396 2307 60-61 0.4662280585758012 2307 62-63 0.4277209040573382 2307 64-65 0.37427218724276656 2307 66-67 0.5512306829661 2307 68-69 0.6743708929757872 2307 70-71 0.4381089831205074 2307 72-73 0.06540308229603653 2307 74-75 -0.00577964669283304 2307 76-77 0.20768194790706573 2307 78-79 0.22745863804728828 2307 80-81 0.2651728307687655 2307 82-83 0.28010888172245885 2307 84-85 0.27480153605347724 2307 86-87 0.2965979492516233 2307 88-89 0.27900140621744995 2307 90-91 0.342130286519307 2307 92-93 0.3152757524715497 2307 94-95 0.20483068498335655 2307 96-97 0.3207088475906055 2307 98-99 0.2747229880481079 2307 100-101 0.2628746687453649 2201 1 0.09028774393345174 2201 2 0.09432209361830246 2201 3 0.08874614829975513 2201 4 0.15962542586051143 2201 5 0.16374265913656672 2201 6 0.1155584370127869 2201 7 0.21623871877054768 2201 8 0.16046792826724499 2201 9 0.23069473968325838 2201 10-11 0.22362642171380998 2201 12-13 0.21170305349429697 2201 14-15 0.46427603817511454 2201 16-17 0.41242762408702305 2201 18-19 0.4458011506112385 2201 20-21 0.5139396753894729 2201 22-23 0.535527185739987 2201 24-25 0.43873313269583747 2201 26-27 0.481870940288907 2201 28-29 0.5632739554355837 2201 30-31 0.4682972408902586 2201 32-33 0.4228436299413687 2201 34-35 0.4830820499409043 2201 36-37 0.5418999447429798 2201 38-39 0.5746429485947289 2201 40-41 0.633245457346419 2201 42-43 0.4805978395583921 2201 44-45 0.528560101094854 2201 46-47 0.6531343387923414 2201 48-49 0.5306356435442439 2201 50-51 0.652120557523169 2201 52-53 0.6391684197352845 2201 54-55 0.7675684204387565 2201 56-57 0.7513670884517438 2201 58-59 0.7273121443750696 2201 60-61 0.6845220033892332 2201 62-63 0.7026264073206221 2201 64-65 0.7087304328737716 2201 66-67 0.8472518445073902 2201 68-69 0.8668398189143289 2201 70-71 0.5897932388566076 2201 72-73 0.18475531372522624 2201 74-75 0.2000827526472193 2201 76-77 0.19201890260756826 2201 78-79 0.4543203073628881 2201 80-81 0.5615500112922653 2201 82-83 0.48149146648749763 2201 84-85 0.49267572335447696 2201 86-87 0.522413171601741 2201 88-89 0.4222791610076406 2201 90-91 0.5085673489553884 2201 92-93 0.5405580595898094 2201 94-95 0.43541110462910737 2201 96-97 0.43475135385596886 2201 98-99 0.4253614516666353 2201 100-101 0.4725553552983932 1101 1 0.08453494436680842 1101 2 0.09088494463126295 1101 3 0.07311513054569474 1101 4 0.12091847905930564 1101 5 0.16351138722902192 1101 6 0.12634383688619977 1101 7 0.18365584257359302 1101 8 0.12270849114139537 1101 9 0.13807109339443002 1101 10-11 0.20917786973028285 1101 12-13 0.23576155142168886 1101 14-15 0.3348517150732846 1101 16-17 0.3301933076935839 1101 18-19 0.3167107020617905 1101 20-21 0.38999845347357365 1101 22-23 0.39791231930605164 1101 24-25 0.4329331517585331 1101 26-27 0.4302539661827254 1101 28-29 0.3275755558704603 1101 30-31 0.3934026977901439 1101 32-33 0.4048720834374535 1101 34-35 0.40507868631721067 1101 36-37 0.4817066968209929 1101 38-39 0.5518850085167344 1101 40-41 0.6125833842675092 1101 42-43 0.5159044396339638 1101 44-45 0.5922987655823775 1101 46-47 0.4779246352284119 1101 48-49 0.5791480755382992 1101 50-51 0.4153634888149753 1101 52-53 0.43226998835145736 1101 54-55 0.5837758273672691 1101 56-57 0.6270063841396478 1101 58-59 0.5695450488927278 1101 60-61 0.5632677823203878 1101 62-63 0.5404634817682279 1101 64-65 0.549420336226838 1101 66-67 0.6562050859864001 1101 68-69 0.6789974717207627 1101 70-71 0.6335080662540022 1101 72-73 0.3217541285260239 1101 74-75 0.36687741512294636 1101 76-77 0.22929943495604377 1101 78-79 0.5830083871619145 1101 80-81 0.7456855111180793 1101 82-83 0.5903867037994956 1101 84-85 0.5885941426050465 1101 86-87 0.6955514505727045 1101 88-89 0.6070606613467042 1101 90-91 0.6287318427832673 1101 92-93 0.665323905696777 1101 94-95 0.5689078415876949 1101 96-97 0.7197239407269329 1101 98-99 0.7365883122942911 1101 100-101 0.5331235980605307 2304 1 0.05242962330500234 2304 2 0.08219643262847143 2304 3 0.0953899357518111 2304 4 0.12500402219588125 2304 5 0.12421088321166707 2304 6 0.13351817669565946 2304 7 0.15239162482617274 2304 8 0.1248417262612449 2304 9 0.09546634190203207 2304 10-11 0.16902773891268197 2304 12-13 0.09528812979936419 2304 14-15 0.17720705074678733 2304 16-17 0.21268649033742548 2304 18-19 0.2482648544175916 2304 20-21 0.2052531509886748 2304 22-23 0.3356319244122332 2304 24-25 0.2637975750437178 2304 26-27 0.23273167189525878 2304 28-29 0.20817634558932951 2304 30-31 0.17435681106683631 2304 32-33 0.06659473436474883 2304 34-35 0.04156909732400038 2304 36-37 0.23375057859122705 2304 38-39 0.26695082128769343 2304 40-41 0.4284200424765885 2304 42-43 0.22671219821217647 2304 44-45 0.32389304994764956 2304 46-47 0.2492581682548476 2304 48-49 0.28530451774648924 2304 50-51 0.32728282455035895 2304 52-53 0.288331160908065 2304 54-55 0.28645844770742457 2304 56-57 0.29654496415842857 2304 58-59 0.30144066355209276 2304 60-61 0.2618893197796055 2304 62-63 0.38879277826406167 2304 64-65 0.3226274603711943 2304 66-67 0.3477495158382524 2304 68-69 0.37305038494795184 2304 70-71 0.39256391184682116 2304 72-73 0.05321569004009774 2304 74-75 0.06046132175023544 2304 76-77 -0.06273495924328643 2304 78-79 0.10283140091753395 2304 80-81 0.08820988399007135 2304 82-83 0.14261246529775207 2304 84-85 0.07250215943771465 2304 86-87 0.0645614097287357 2304 88-89 -0.02434946558590312 2304 90-91 0.006752236691507818 2304 92-93 0.04961221325050147 2304 94-95 -0.05009882277548172 2304 96-97 -0.06588338700696994 2304 98-99 0.04878049985338251 2304 100-101 0.0759850210786972 2202 1 0.058270724338505886 2202 2 0.08122845372103882 2202 3 0.0883557324566624 2202 4 0.12563766458872294 2202 5 0.10245563454051165 2202 6 0.06102665359450299 2202 7 0.13146719466644896 2202 8 0.042870487959163484 2202 9 0.1180719850284504 2202 10-11 0.13386258234118742 2202 12-13 0.1537871674871809 2202 14-15 0.15411928560865817 2202 16-17 0.0697711210063332 2202 18-19 0.10209324576955936 2202 20-21 0.18573261865693524 2202 22-23 0.1334807471252688 2202 24-25 0.14239133840559504 2202 26-27 0.07443617894724497 2202 28-29 0.11900644030598784 2202 30-31 0.0657051389721417 2202 32-33 0.2028684495904045 2202 34-35 0.1743795447476515 2202 36-37 0.2078404968255967 2202 38-39 0.13328994721149456 2202 40-41 0.16077525905349432 2202 42-43 0.148280696242729 2202 44-45 0.21270645411154732 2202 46-47 0.256866674363053 2202 48-49 0.17443599880058258 2202 50-51 0.20674166432537078 2202 52-53 0.3406953296156132 2202 54-55 0.34514674098391396 2202 56-57 0.27809515224203096 2202 58-59 0.2832495245515574 2202 60-61 0.2581894341958417 2202 62-63 0.3588759064792839 2202 64-65 0.31222906029247355 2202 66-67 0.27628502144882106 2202 68-69 0.18378133781216377 2202 70-71 0.21020707252720428 2202 72-73 0.07677359980323217 2202 74-75 0.045617797936099436 2202 76-77 0.020050161803016664 2202 78-79 0.10134552882469094 2202 80-81 0.0924300453576663 2202 82-83 0.08895853810583532 2202 84-85 0.07113474112349039 2202 86-87 -0.08003832790209842 2202 88-89 -0.03751071392323624 2202 90-91 -0.10438509731221757 2202 92-93 -0.10575303780067458 2202 94-95 -0.12377073021423612 2202 96-97 -0.056173525676772584 2202 98-99 0.026180555644824466 2202 100-101 0.23641339038196918 1102 1 0.07280978009968209 1102 2 0.037654429205559836 1102 3 -1.9445653548189057E-4 1102 4 0.037813769259010144 1102 5 0.0585778986341694 1102 6 0.056495578674741864 1102 7 0.0902364616074749 1102 8 0.10985853781188837 1102 9 0.06978763766411333 1102 10-11 0.05195492441426808 1102 12-13 0.034262382794416624 1102 14-15 0.10679828886233622 1102 16-17 0.16334116508785712 1102 18-19 0.10746172409959343 1102 20-21 0.014170151381385665 1102 22-23 0.04996907418169627 1102 24-25 0.023030564711952195 1102 26-27 0.04478123263744038 1102 28-29 0.11041960278787855 1102 30-31 0.023417236894808013 1102 32-33 0.07148847736498709 1102 34-35 0.011580024225871455 1102 36-37 -0.07604493743974672 1102 38-39 -0.04473272157387953 1102 40-41 0.025550958033392135 1102 42-43 0.04180959490658864 1102 44-45 0.001876706259913874 1102 46-47 0.08530745991524924 1102 48-49 -0.008171673364600451 1102 50-51 -0.053176535616820786 1102 52-53 -0.05832574043346028 1102 54-55 -0.01576705251802224 1102 56-57 0.06885329793618666 1102 58-59 0.02874973669870684 1102 60-61 -0.1861208992675074 1102 62-63 0.03282104154724408 1102 64-65 -0.07782225163858669 1102 66-67 -0.009360848900882957 1102 68-69 -0.04105525752721206 1102 70-71 -0.20499450435238487 1102 72-73 -0.1857912891169775 1102 74-75 -0.28072388650936375 1102 76-77 -0.2127899876786863 1102 78-79 -0.0689921917362959 1102 80-81 0.0737492838649807 1102 82-83 -0.037378638034500256 1102 84-85 -0.1188938295469022 1102 86-87 -0.09314308636109203 1102 88-89 -0.2548053713248599 1102 90-91 -0.24475868172580562 1102 92-93 -0.15908446128660714 1102 94-95 -0.33447544393258255 1102 96-97 -0.24151203233779484 1102 98-99 -0.16704075106173732 1102 100-101 -0.20547079011801372 2305 1 0.07417935299418588 2305 2 0.03926046381804582 2305 3 0.06816263865872685 2305 4 0.07844561402014705 2305 5 0.07807223434495825 2305 6 0.04416055594310819 2305 7 0.0012884502520407182 2305 8 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1213 96-97 -0.07065728932537141 1213 98-99 0.13395389046145922 1213 100-101 0.20772348218537573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 13.0 8 82.0 9 272.0 10 193.0 11 96.0 12 78.0 13 163.0 14 379.0 15 864.0 16 1728.0 17 2924.0 18 4487.0 19 6621.0 20 9476.0 21 13322.0 22 18045.0 23 25066.0 24 33478.0 25 44859.0 26 63125.0 27 94626.0 28 163731.0 29 263871.0 30 216126.0 31 119738.0 32 134176.0 33 189461.0 34 290010.0 35 499114.0 36 955685.0 37 1362703.0 38 307562.0 39 62.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.3580736541555 23.044339347831237 19.82791757030962 13.769669427703645 2 26.656382150980395 32.36673540522291 28.37076764321869 12.606114800578 3 32.675706367468685 17.27122586339332 34.50651329618244 15.546554472955554 4 25.924574570100024 17.205001423994084 28.757793852535613 28.112630153370283 5 40.62060879245214 16.081690769401774 27.52023169815202 15.77746873999406 6 35.65328725693344 17.13220033611661 31.43623489673456 15.778277510215391 7 28.638989028928258 29.86765615901335 24.371461111839235 17.121893700219157 8 25.17929813675931 35.81960359475552 22.28518648167534 16.71591178680983 9 37.982649182851745 21.831113846643895 22.659854471130636 17.52638249937372 10-11 27.137486375332426 31.639619869700898 25.792511866110786 15.430381888855893 12-13 20.634237607566437 30.32061932720272 25.719961029717954 23.325182035512892 14-15 20.218571189199146 30.78567257331606 26.081512425199122 22.914243812285676 16-17 25.98379639230416 24.865754512108325 26.60400909472483 22.546440000862688 18-19 18.875380122004024 25.006926391126257 33.094815243701134 23.022878243168588 20-21 25.21577574751106 25.166077854295278 32.762524740073694 16.855621658119972 22-23 18.61570266786337 37.81240305126193 26.49407855771799 17.077815723156707 24-25 18.82689538412023 24.584416532424637 32.96301680417143 23.625671279283704 26-27 18.737920290925018 24.79634543695989 26.393459247105433 30.072275025009667 28-29 24.523541019996117 31.630598971078378 26.146348837942355 17.699511170983147 30-31 17.599710999440912 26.211257832628526 32.21729955355884 23.971731614371723 32-33 17.38866344706993 32.39351814216771 26.473828195637783 23.743990215124583 34-35 17.839480263518077 32.31933939648322 33.15374141251927 16.68743892747944 36-37 17.7574522991471 26.5232461299308 32.96033127228266 22.75897029863944 38-39 24.84992127969846 32.41749092103582 25.741414178281158 16.991173620984558 40-41 18.299069126212945 38.254178231389574 25.69464030048095 17.75211234191653 42-43 18.332342762626354 31.990408399928995 32.40876035018506 17.268488487259585 44-45 17.762097543495248 25.697564315896525 32.877919660499 23.662418480109228 46-47 24.364751676126524 25.6513200598076 26.46546235792067 23.51846590614521 48-49 18.432181794634285 32.34699687482404 31.944194575306746 17.276626755234922 50-51 24.777406318829843 25.783352450070268 31.489993938359568 17.94924729274032 52-53 18.190939267033514 25.757549677581515 25.44568699172998 30.605824063654996 54-55 24.828249162892902 25.819079094047385 31.701922468690796 17.650749274368916 56-57 18.413784664141144 25.776981796950913 31.699345320749277 24.109888218158666 58-59 18.327083790062634 25.80157418386579 32.123789697597324 23.747552328474253 60-61 24.358353269868235 26.122684762735876 31.885922926074993 17.633039041320888 62-63 17.909076562008437 26.239840083384262 31.970807627436926 23.880275727170375 64-65 24.550935336638023 26.1461106641671 31.748289428083805 17.554664571111072 66-67 18.809077821304633 38.08309354614502 25.24434855491965 17.86348007763069 68-69 18.874020252708736 38.221034473746116 25.2159354110427 17.689009862502452 70-71 18.33181759396417 38.970068840391455 25.305815844770862 17.392297720873508 72-73 18.197648516083643 38.810301213615595 25.163908210572426 17.82814205972834 74-75 18.948486757403074 37.49902252009979 25.069056232525927 18.483434489971213 76-77 19.291036653842838 35.87395972114527 25.67128572653773 19.163717898474157 78-79 18.98186611561404 35.014461398456426 26.512619141142668 19.491053344786867 80-81 18.5102515704945 34.88651989327595 26.390435773273925 20.21279276295563 82-83 17.990682066046343 34.41228601901371 26.653973715595637 20.94305819934431 84-85 18.339570545372307 33.70279279373629 26.948841450522753 21.008795210368643 86-87 18.440382452135147 33.542651800741346 27.52907036736407 20.48789537975943 88-89 18.746361934581245 33.78399974032522 27.219537498894258 20.250100826199276 90-91 19.11048176620159 33.05136916050003 26.853636585948138 20.984512487350244 92-93 18.66229336279198 33.582557864225905 26.57441687754856 21.180731895433553 94-95 18.417270857616 33.785947205234656 26.412575814033463 21.38420612311588 96-97 18.945593791214723 34.061099503159866 26.125481820434192 20.867824885191215 98-99 19.428533793703583 32.92159997676713 26.07226146513281 21.577604764396465 100-101 19.45641930701022 33.16626658659189 25.597519686750108 21.77979441964779 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 84.5 2 153.5 3 357.0 4 362.0 5 218.0 6 189.5 7 194.0 8 194.0 9 199.5 10 181.5 11 165.5 12 181.5 13 185.0 14 182.5 15 188.0 16 214.5 17 266.0 18 316.5 19 408.5 20 602.5 21 882.5 22 1285.5 23 1943.0 24 2819.5 25 3974.5 26 5614.0 27 7859.0 28 10674.5 29 14069.0 30 18018.0 31 23164.5 32 31624.5 33 49843.5 34 81155.0 35 133164.0 36 203084.5 37 285292.0 38 369509.5 39 410911.5 40 411171.0 41 376123.0 42 329697.5 43 316907.0 44 296934.5 45 265009.5 46 251895.0 47 223004.0 48 169007.0 49 123403.0 50 93521.5 51 75165.0 52 61180.5 53 47526.0 54 36164.0 55 25842.0 56 17317.0 57 11997.5 58 8714.5 59 6349.0 60 4652.5 61 3498.5 62 2510.5 63 1611.5 64 1037.5 65 654.5 66 427.0 67 306.0 68 212.0 69 147.0 70 91.5 71 67.0 72 51.5 73 38.5 74 29.0 75 20.0 76 17.0 77 12.0 78 10.5 79 10.5 80 5.5 81 3.0 82 3.5 83 2.0 84 0.5 85 0.5 86 0.5 87 0.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19099419842161233 2 0.0 3 0.0 4 0.02465712290155234 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.2442618789681585E-4 48-49 0.0034735644121194425 50-51 1.8663928184522378E-4 52-53 0.002944753113557975 54-55 0.009767455749900044 56-57 0.014329749306116624 58-59 0.014868929453669494 60-61 0.0034839332611108437 62-63 0.0025507368518847247 64-65 0.010431062085349729 66-67 0.017564830191433838 68-69 0.0259221224785033 70-71 0.02190937791883099 72-73 0.017419666305554218 74-75 0.022085648351684813 76-77 0.01601987169171504 78-79 0.006926391126256083 80-81 0.0034735644121194425 82-83 7.672948253636977E-4 84-85 0.0028306957746525607 86-87 0.016061347087680644 88-89 0.014941511396609304 90-91 0.01860171509057397 92-93 0.018964624805273014 94-95 0.01828028077184053 96-97 0.020063722798361557 98-99 0.028586916669293444 100-101 0.01678716651707874 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4822136.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.354973297584756 #Duplication Level Percentage of deduplicated Percentage of total 1 87.9621757721945 29.339760240790042 2 5.248365659059486 3.5011819282777994 3 1.8015113611176112 1.8026809003612045 4 1.0179322488396343 1.3581241191518563 5 0.711320424120922 1.1863036876290007 6 0.37889416341686133 0.7582802822028074 7 0.32122241015365216 0.7500055439282621 8 0.27109773361275646 0.7233966132551389 9 0.24736022010926864 0.7425624182966408 >10 1.548970075412686 10.368306195373258 >50 0.23125526427944487 5.45281569254661 >100 0.20631467945409881 14.212529056298445 >500 0.03551976728596426 8.372840503904499 >1k 0.017621569998729575 9.182434768281833 >5k 3.759866094814454E-4 0.867671248503789 >10k+ 6.266433485205888E-5 11.381106801198861 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT 544507 11.291821715521918 TruSeq Adapter, Index 13 (97% over 38bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8919 0.18495952830861676 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATATCGTAT 7226 0.1498506056237319 TruSeq Adapter, Index 13 (97% over 38bp) TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7179 0.14887593381854017 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6773 0.1404564284375223 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6368 0.13205766075448722 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 5047 0.10466316171920494 No Hit GTGTTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAAT 4932 0.10227832645118262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.00620057169685799 0.0 2.073769798280264E-5 2 0.00659458795853124 0.0 2.073769798280264E-5 3 0.007175243502049714 0.0 2.073769798280264E-5 4 0.0076314728576713725 0.0 2.073769798280264E-5 5 0.008502456172949082 0.0 2.073769798280264E-5 6 0.009269750998312782 0.0 2.073769798280264E-5 7 0.010410324387366927 0.0 2.073769798280264E-5 8 0.01153016007843827 0.0 2.073769798280264E-5 9 0.012753684259423624 0.0 4.147539596560528E-5 10-11 0.019244583728040852 0.0 6.221309394840793E-5 12-13 0.023568393757455203 0.0 6.221309394840793E-5 14-15 0.028825400196095672 0.0 8.295079193121056E-5 16-17 0.033024784037613206 0.0 8.295079193121056E-5 18-19 0.03759744644282119 0.0 8.295079193121056E-5 20-21 0.042916665975410065 0.0 1.1405733890541454E-4 22-23 0.04864027061866359 0.0 1.3479503688821717E-4 24-25 0.05484084231552158 0.0 1.451638858796185E-4 26-27 0.06154948761295824 0.0 1.451638858796185E-4 28-29 0.06891137039685317 0.0 1.451638858796185E-4 30-31 0.10610443172900971 0.0 1.451638858796185E-4 32-33 0.11594446942184958 0.0 1.6590158386242112E-4 34-35 0.12400106508816841 0.0 1.6590158386242112E-4 36-37 0.13242057046918626 0.0 1.6590158386242112E-4 38-39 0.14119261671591177 0.0 1.6590158386242112E-4 40-41 0.15188290002604654 0.0 1.6590158386242112E-4 42-43 0.1626768718260953 0.0 1.6590158386242112E-4 44-45 0.17331531089127308 0.0 1.6590158386242112E-4 46-47 0.18489731521466835 0.0 1.6590158386242112E-4 48-49 0.1978480076049286 0.0 1.6590158386242112E-4 50-51 0.21180447834735477 0.0 1.6590158386242112E-4 52-53 0.2260098014655746 0.0 1.7627043285382246E-4 54-55 0.2409409440131925 0.0 1.8663928184522378E-4 56-57 0.25670159448012253 0.0 1.8663928184522378E-4 58-59 0.2741108919366853 0.0 1.8663928184522378E-4 60-61 0.2925363365944055 0.0 2.0737697982802641E-4 62-63 0.31282817407057784 0.0 2.0737697982802641E-4 64-65 0.3338665686741311 0.0 2.1774582881942773E-4 66-67 0.3574971755255347 0.0 2.2811467781082905E-4 68-69 0.3824238885008635 0.0 2.3848352680223036E-4 70-71 0.4104301496266385 0.0 2.6959007377643434E-4 72-73 0.4404583363057367 0.0 2.7995892276783563E-4 74-75 0.47376307926611777 0.0 2.90327771759237E-4 76-77 0.5109146652023087 0.0 3.006966207506383E-4 78-79 0.5510317419500403 0.0 3.3180316772484224E-4 80-81 0.596519882475318 0.0 3.3180316772484224E-4 82-83 0.6481774881504794 0.0 3.5254086570764493E-4 84-85 0.7074561978343207 0.0 3.5254086570764493E-4 86-87 0.7718985943158799 0.0 3.5254086570764493E-4 88-89 0.8427800460210993 0.0 3.7327856369044756E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 67785 0.0 86.814575 1 TCGGAAG 67815 0.0 86.30017 3 CGGAAGA 67945 0.0 85.876366 4 ATCGGAA 68375 0.0 85.63504 2 GAAGAGC 68350 0.0 85.229034 6 GGAAGAG 70740 0.0 82.54421 5 TCGGACG 300 0.0 72.83888 3 GAGCACA 86420 0.0 67.51247 9 AGAGCAC 86320 0.0 67.48615 8 AAGAGCA 87000 0.0 66.90954 7 ATCGGAC 320 0.0 66.80196 2 GCGCGTC 30 1.2877234E-4 63.471134 1 CGAGCAC 400 0.0 52.24152 8 GATCGGC 255 0.0 46.66995 1 CGGACGA 510 0.0 46.572174 4 TTGTGTT 42475 0.0 46.52184 1 GCCGTCT 63450 0.0 46.01089 50-51 CGTCTTC 63455 0.0 45.97572 52-53 ATGCCGT 63460 0.0 45.95005 48-49 TCGTATG 63925 0.0 45.83001 44-45 >>END_MODULE