##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_52370180-2.341000000358c0.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5395423 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70377670110388 33.0 33.0 33.0 33.0 33.0 2 32.72805728114366 33.0 33.0 33.0 33.0 33.0 3 32.76543470271005 33.0 33.0 33.0 33.0 33.0 4 36.68930517588704 37.0 37.0 37.0 37.0 37.0 5 36.69918632885688 37.0 37.0 37.0 37.0 37.0 6 36.578824866928876 37.0 37.0 37.0 37.0 37.0 7 36.45633030070117 37.0 37.0 37.0 37.0 37.0 8 36.39915109528947 37.0 37.0 37.0 37.0 37.0 9 36.480472059373284 37.0 37.0 37.0 37.0 37.0 10-11 36.40572741006591 37.0 37.0 37.0 37.0 37.0 12-13 36.39111409800492 37.0 37.0 37.0 37.0 37.0 14-15 38.27151253942462 40.0 37.0 40.0 37.0 40.0 16-17 38.30784666929729 40.0 37.0 40.0 37.0 40.0 18-19 38.27187951713888 40.0 37.0 40.0 37.0 40.0 20-21 38.29455827652438 40.0 37.0 40.0 37.0 40.0 22-23 38.0958839557158 40.0 37.0 40.0 37.0 40.0 24-25 38.16322584902055 40.0 37.0 40.0 37.0 40.0 26-27 38.12026313784851 40.0 37.0 40.0 37.0 40.0 28-29 37.93035745297449 40.0 37.0 40.0 33.0 40.0 30-31 37.9597835980608 40.0 37.0 40.0 35.0 40.0 32-33 37.83588997563305 40.0 37.0 40.0 33.0 40.0 34-35 37.65792079694215 40.0 37.0 40.0 33.0 40.0 36-37 37.32935740163468 40.0 37.0 40.0 33.0 40.0 38-39 37.57208841642259 40.0 37.0 40.0 33.0 40.0 40-41 37.348548853352185 40.0 37.0 40.0 33.0 40.0 42-43 37.14787793283307 40.0 37.0 40.0 33.0 40.0 44-45 37.21708214536655 40.0 37.0 40.0 33.0 40.0 46-47 37.268889575479065 40.0 37.0 40.0 33.0 40.0 48-49 36.95501844804383 37.0 37.0 40.0 33.0 40.0 50-51 36.90622014251709 37.0 37.0 40.0 33.0 40.0 52-53 36.85327878833597 37.0 37.0 40.0 33.0 40.0 54-55 36.57012026675203 37.0 37.0 40.0 33.0 40.0 56-57 36.49088885153212 37.0 37.0 40.0 33.0 40.0 58-59 36.21946851989176 37.0 37.0 40.0 33.0 40.0 60-61 35.95474757030172 37.0 37.0 40.0 33.0 40.0 62-63 35.75959716226142 37.0 37.0 40.0 33.0 40.0 64-65 35.5143573543724 37.0 35.0 40.0 30.0 40.0 66-67 34.89328519747201 37.0 33.0 37.0 27.0 40.0 68-69 34.763898771236285 37.0 33.0 37.0 27.0 40.0 70-71 34.298174397076934 37.0 33.0 37.0 27.0 40.0 72-73 33.40633987362993 37.0 33.0 37.0 24.5 40.0 74-75 32.588033690778275 37.0 33.0 37.0 22.0 38.5 76-77 30.16879038029085 33.0 30.0 35.0 18.5 37.0 78-79 32.118969170721186 35.0 33.0 37.0 22.0 37.0 80-81 32.338007233167815 37.0 33.0 37.0 15.0 37.0 82-83 32.20221074418076 37.0 33.0 37.0 15.0 37.0 84-85 31.99434798717357 37.0 33.0 37.0 15.0 37.0 86-87 31.86598743045726 37.0 33.0 37.0 15.0 37.0 88-89 31.72976270813243 37.0 33.0 37.0 15.0 37.0 90-91 31.57252628755892 35.0 33.0 37.0 15.0 37.0 92-93 31.436060990954743 33.0 33.0 37.0 15.0 37.0 94-95 31.319044679166026 33.0 33.0 37.0 15.0 37.0 96-97 31.168410799301554 33.0 33.0 37.0 15.0 37.0 98-99 31.00433969681339 33.0 33.0 37.0 15.0 37.0 100-101 29.528837498005252 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.14436502796444728 2306 2 0.10900550418349297 2306 3 0.11882791222626565 2306 4 0.16803097512872966 2306 5 0.16972951279277737 2306 6 0.17690372187114178 2306 7 0.18118518497528413 2306 8 0.20771935033320688 2306 9 0.1932948154589056 2306 10-11 0.24482215661223705 2306 12-13 0.21710949412442204 2306 14-15 0.5207149478221211 2306 16-17 0.44072017456851853 2306 18-19 0.3793265080963266 2306 20-21 0.4906082050375531 2306 22-23 0.48698221155726884 2306 24-25 0.4291630610570607 2306 26-27 0.4660551345752211 2306 28-29 0.5233552986765702 2306 30-31 0.43879928888733133 2306 32-33 0.5067021751470762 2306 34-35 0.5316447074725872 2306 36-37 0.507875535230859 2306 38-39 0.48011723199771694 2306 40-41 0.523354495306144 2306 42-43 0.5324517020370862 2306 44-45 0.5458675013118324 2306 46-47 0.5500559134435221 2306 48-49 0.5856397732486087 2306 50-51 0.5375166888242617 2306 52-53 0.5802603490601044 2306 54-55 0.599532279443558 2306 56-57 0.6493524535204713 2306 58-59 0.5826622679628386 2306 60-61 0.5870966228809564 2306 62-63 0.5533070255621197 2306 64-65 0.4114122868714887 2306 66-67 0.506115824605935 2306 68-69 0.6829503697768615 2306 70-71 0.5981890603356277 2306 72-73 0.06167247431496037 2306 74-75 0.09611539420271242 2306 76-77 0.36256293195715017 2306 78-79 0.3494009834680156 2306 80-81 0.34302851631785103 2306 82-83 0.42149307120737944 2306 84-85 0.5385580301150057 2306 86-87 0.4180502994683053 2306 88-89 0.34443942839262576 2306 90-91 0.4199153830238025 2306 92-93 0.44399794683895166 2306 94-95 0.3960984531336962 2306 96-97 0.37822812246341897 2306 98-99 0.520454623554766 2306 100-101 0.421400328122143 2307 1 0.11115437547097429 2307 2 0.10756706596480115 2307 3 0.11493870084782287 2307 4 0.13491789099661844 2307 5 0.13596359268837688 2307 6 0.17516859747590985 2307 7 0.1685767491793726 2307 8 0.1735469164537804 2307 9 0.17827857702508965 2307 10-11 0.22590226744079445 2307 12-13 0.21593864219212122 2307 14-15 0.4577078719673935 2307 16-17 0.41736057739946375 2307 18-19 0.4195783861293094 2307 20-21 0.45131295824710094 2307 22-23 0.5451584528776934 2307 24-25 0.45407629535850447 2307 26-27 0.4918143510254538 2307 28-29 0.4220716617628213 2307 30-31 0.4536681321120355 2307 32-33 0.5328201954546827 2307 34-35 0.5594597158372991 2307 36-37 0.5426002666998571 2307 38-39 0.5599093835835589 2307 40-41 0.5627199799422229 2307 42-43 0.5546119161111704 2307 44-45 0.5563920247782264 2307 46-47 0.5872669003487658 2307 48-49 0.573619022530842 2307 50-51 0.5896379096774496 2307 52-53 0.559748022902717 2307 54-55 0.5587395731588245 2307 56-57 0.624462979389925 2307 58-59 0.6312755144350533 2307 60-61 0.6765770473437698 2307 62-63 0.5235880439281004 2307 64-65 0.40924755204574126 2307 66-67 0.5320572234303924 2307 68-69 0.6228683226207608 2307 70-71 0.3653662736745389 2307 72-73 0.13682011281306927 2307 74-75 0.19922553106255947 2307 76-77 0.4156380352033082 2307 78-79 0.4675976282032863 2307 80-81 0.4387154323373963 2307 82-83 0.48703679807359634 2307 84-85 0.5817923151316506 2307 86-87 0.4973970110416843 2307 88-89 0.358595434894319 2307 90-91 0.40026739627489505 2307 92-93 0.5385090503585985 2307 94-95 0.3855849566615106 2307 96-97 0.5601972238777684 2307 98-99 0.5522449739174426 2307 100-101 0.4163730614302743 2201 1 0.10503254516464722 2201 2 0.09384932875408936 2201 3 0.10489880109156502 2201 4 0.1540874236963603 2201 5 0.11114125405441655 2201 6 0.12722801485612223 2201 7 0.13309105156282186 2201 8 0.17748786514136583 2201 9 0.1615485430412491 2201 10-11 0.22670590066373109 2201 12-13 0.20097173946074776 2201 14-15 0.3730430487135905 2201 16-17 0.3268983187447816 2201 18-19 0.3300018808184646 2201 20-21 0.38526713603675944 2201 22-23 0.4099125378430202 2201 24-25 0.4394875128853144 2201 26-27 0.442578640130769 2201 28-29 0.47807431575439097 2201 30-31 0.4197510922278056 2201 32-33 0.48990630391698176 2201 34-35 0.46958373764774564 2201 36-37 0.36088233011292914 2201 38-39 0.509209197199489 2201 40-41 0.5343681103408002 2201 42-43 0.5122555017126089 2201 44-45 0.5526800727461776 2201 46-47 0.5839079422692492 2201 48-49 0.5127669915490927 2201 50-51 0.6400698778557015 2201 52-53 0.6331188781858188 2201 54-55 0.6001561003030034 2201 56-57 0.6316638108867991 2201 58-59 0.49793797295622255 2201 60-61 0.6160014057241483 2201 62-63 0.6367712240931098 2201 64-65 0.6147423278169555 2201 66-67 0.6994098586691635 2201 68-69 0.785342368985539 2201 70-71 0.5302297704228351 2201 72-73 0.22402943414133603 2201 74-75 0.3814997191151832 2201 76-77 0.18007258710117924 2201 78-79 0.4340754594476621 2201 80-81 0.5619419850000966 2201 82-83 0.46684607511419784 2201 84-85 0.5112629794548269 2201 86-87 0.5116391322085008 2201 88-89 0.5280335535019205 2201 90-91 0.6434307940704791 2201 92-93 0.6077269154479126 2201 94-95 0.5238472540474746 2201 96-97 0.4556160242376244 2201 98-99 0.5081865076598255 2201 100-101 0.5549129738518239 1101 1 0.11754771218162574 1101 2 0.10452939523441529 1101 3 0.06969428041217185 1101 4 0.08361332533338839 1101 5 0.1320255170008906 1101 6 0.17151570443110842 1101 7 0.16783663634114987 1101 8 0.1540298313569579 1101 9 0.1627188724601254 1101 10-11 0.17807091343559023 1101 12-13 0.1479997168663587 1101 14-15 0.32103289446204286 1101 16-17 0.3143286249553441 1101 18-19 0.294404311871169 1101 20-21 0.321857992537403 1101 22-23 0.32815745313440203 1101 24-25 0.2951448303138662 1101 26-27 0.2577375973280809 1101 28-29 0.307900251291791 1101 30-31 0.3589193368215646 1101 32-33 0.2980672656631427 1101 34-35 0.36618985735756837 1101 36-37 0.39143055916374436 1101 38-39 0.32076550221458433 1101 40-41 0.4503498296441606 1101 42-43 0.37748777397370503 1101 44-45 0.4294917147131514 1101 46-47 0.35520505460640095 1101 48-49 0.4202933634828767 1101 50-51 0.43842762257052215 1101 52-53 0.3932005434822159 1101 54-55 0.4089613621820902 1101 56-57 0.397714215167305 1101 58-59 0.46673025130073853 1101 60-61 0.42046389821767605 1101 62-63 0.48488835971145505 1101 64-65 0.3449216718644337 1101 66-67 0.4015473295850924 1101 68-69 0.5079971256117801 1101 70-71 0.4878060390424537 1101 72-73 0.19786186187474186 1101 74-75 0.1596565941668331 1101 76-77 0.12813067839726955 1101 78-79 0.37773968427952553 1101 80-81 0.39249997260153435 1101 82-83 0.38112921177067705 1101 84-85 0.4002880557682005 1101 86-87 0.39677957120287033 1101 88-89 0.2850104616951299 1101 90-91 0.47508700713764185 1101 92-93 0.521792293342827 1101 94-95 0.46939612241465056 1101 96-97 0.5584345733935514 1101 98-99 0.6639770794101452 1101 100-101 0.5026147521159032 2304 1 0.011333782964335626 2304 2 0.04665073895908023 2304 3 0.07883879825195095 2304 4 0.1123773452509127 2304 5 0.10471889790935762 2304 6 0.10915143295987662 2304 7 0.040398262906052196 2304 8 0.10870301412396799 2304 9 0.11398413527725637 2304 10-11 0.1210450978469666 2304 12-13 0.14213770636472844 2304 14-15 0.27315601247453714 2304 16-17 0.2520326106764159 2304 18-19 0.27377110566519036 2304 20-21 0.2655626486558731 2304 22-23 0.2637796942814745 2304 24-25 0.26303296475898463 2304 26-27 0.2623258976860754 2304 28-29 0.2671016601042737 2304 30-31 0.24159091135332744 2304 32-33 0.11121888983505102 2304 34-35 0.18969801359309457 2304 36-37 0.23868376101483335 2304 38-39 0.23244720675235442 2304 40-41 0.24163482817024118 2304 42-43 0.21985285275587785 2304 44-45 0.20603582911699903 2304 46-47 0.22797554418809796 2304 48-49 0.2776741513983936 2304 50-51 0.24168345072684616 2304 52-53 0.21349607117917202 2304 54-55 0.2667207829220217 2304 56-57 0.24156535980250027 2304 58-59 0.2232308756943837 2304 60-61 0.22238002928824585 2304 62-63 0.23669101319982389 2304 64-65 0.1254135018577287 2304 66-67 0.19159718727283348 2304 68-69 0.2020273006795037 2304 70-71 0.1637363576299009 2304 72-73 0.03477958760895916 2304 74-75 0.018950155178487194 2304 76-77 -0.03359837707287383 2304 78-79 0.27480369695221185 2304 80-81 0.15901160197056896 2304 82-83 0.19420846236258882 2304 84-85 0.2453812018878807 2304 86-87 0.22124527405935623 2304 88-89 0.17649335119371656 2304 90-91 0.22802503519533346 2304 92-93 0.2990015411545528 2304 94-95 0.1757214735150754 2304 96-97 0.3125294560799965 2304 98-99 0.2470244028001467 2304 100-101 0.18131337884905108 2202 1 0.04326303140409493 2202 2 0.05120383375620463 2202 3 0.06212046694949436 2202 4 0.06283312621238935 2202 5 0.05475826434772557 2202 6 0.07818318336567387 2202 7 0.13107162125691474 2202 8 0.07023161292595148 2202 9 0.10976158123766311 2202 10-11 0.12204173247423711 2202 12-13 0.0904927614273916 2202 14-15 0.1325379502371149 2202 16-17 0.06836185508798565 2202 18-19 0.14269713887398439 2202 20-21 0.1551102413066161 2202 22-23 0.16674687091136065 2202 24-25 0.17219037649805102 2202 26-27 0.18301817939230602 2202 28-29 0.14877571331854256 2202 30-31 0.14047107350643273 2202 32-33 0.18249191805712428 2202 34-35 0.18139764583731477 2202 36-37 0.1960537304646479 2202 38-39 0.22215395882893318 2202 40-41 0.19224777734817167 2202 42-43 0.22486111055295055 2202 44-45 0.19430810937674892 2202 46-47 0.3117582194763173 2202 48-49 0.3508438180012874 2202 50-51 0.1300622034699117 2202 52-53 0.2057753513409537 2202 54-55 0.22328886717718177 2202 56-57 0.275780516613672 2202 58-59 0.18824054335924956 2202 60-61 0.11879859889498334 2202 62-63 0.20045899015855895 2202 64-65 0.14675828227996845 2202 66-67 0.1378158577674995 2202 68-69 0.14533576144260962 2202 70-71 0.11159908045080869 2202 72-73 0.0626173127414944 2202 74-75 0.13386914271031003 2202 76-77 0.10731390351644876 2202 78-79 0.2706740202422182 2202 80-81 0.303463337343878 2202 82-83 0.24317575709178385 2202 84-85 0.37876453670127574 2202 86-87 0.21717931604960938 2202 88-89 0.16655776567287717 2202 90-91 0.21921965554265554 2202 92-93 0.3199422662843503 2202 94-95 0.1496598613770317 2202 96-97 0.33338112608670656 2202 98-99 0.2537819952586382 2202 100-101 0.2629094773603562 1102 1 0.09751769593536608 1102 2 0.09546170803713494 1102 3 0.055737305994689734 1102 4 0.10811092755050566 1102 5 0.0928930006576465 1102 6 0.11010431000443077 1102 7 0.10995828715856248 1102 8 0.10187184489272738 1102 9 0.04155007702923541 1102 10-11 0.10420215592836257 1102 12-13 0.09115878448104553 1102 14-15 0.13315651306754717 1102 16-17 0.15845374872386486 1102 18-19 0.10816268562074782 1102 20-21 -9.163131352281084E-4 1102 22-23 0.14785959382680858 1102 24-25 0.04494340621899795 1102 26-27 0.10840711207570308 1102 28-29 0.1762826234272623 1102 30-31 0.14600346370555428 1102 32-33 0.14230011472663762 1102 34-35 0.08492398407840795 1102 36-37 0.03988138603355651 1102 38-39 0.13698630761729902 1102 40-41 0.07386956988525384 1102 42-43 0.059598064916293936 1102 44-45 0.20415913042638323 1102 46-47 0.1538528596933162 1102 48-49 0.17684561745679162 1102 50-51 0.05267782098083984 1102 52-53 0.09985629440864585 1102 54-55 0.08557052520414032 1102 56-57 0.1880824226727853 1102 58-59 0.13694491876358228 1102 60-61 0.03928804829818233 1102 62-63 0.152128163922427 1102 64-65 0.0457555889175012 1102 66-67 0.10316804463813156 1102 68-69 0.15010722893017459 1102 70-71 0.09418762297499939 1102 72-73 2.004253006049339E-4 1102 74-75 -0.03224475683617101 1102 76-77 0.05625903093353202 1102 78-79 0.21101090675178824 1102 80-81 0.1756030221564373 1102 82-83 0.06035575502956192 1102 84-85 0.12086219125571063 1102 86-87 0.10471667304528864 1102 88-89 0.06592714549270084 1102 90-91 0.12712780098921783 1102 92-93 0.0640508373137827 1102 94-95 -0.08816263115156886 1102 96-97 0.13954245259474973 1102 98-99 0.1569896868499221 1102 100-101 0.14464704124352679 2305 1 0.07704882074018116 2305 2 0.05132967981248271 2305 3 0.03718823594544318 2305 4 0.05369626514703185 2305 5 0.061695900966739714 2305 6 0.05526747139607835 2305 7 -0.0044274040822784855 2305 8 0.05048372253726541 2305 9 0.05519031767765625 2305 10-11 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0.21347836868801906 1213 100-101 0.18853394129286372 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 16.0 10 27.0 11 22.0 12 39.0 13 101.0 14 191.0 15 625.0 16 1421.0 17 2551.0 18 4165.0 19 6260.0 20 8809.0 21 12277.0 22 16874.0 23 22403.0 24 29766.0 25 40233.0 26 56088.0 27 87527.0 28 156470.0 29 219038.0 30 144160.0 31 103780.0 32 132781.0 33 190465.0 34 292819.0 35 512364.0 36 1029826.0 37 1723812.0 38 600141.0 39 365.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.83341785870342 24.368636339917153 22.984264910618833 12.813680890760601 2 26.898669483375077 29.69483578952012 32.07683623693638 11.329658490168427 3 34.46814086680507 18.190547803202826 32.58812145034782 14.753189879644282 4 25.423471464897585 18.52262185232893 33.6461260203026 22.407780662470884 5 36.60409943761592 17.488563917972698 31.863599943878356 14.043736700533028 6 30.353338375878963 18.08013940704927 37.339315193637276 14.227207023434493 7 29.982320199917595 26.448806701532018 28.361983851868516 15.206889246681863 8 25.360291491510488 33.93618998918157 26.223930913294474 14.479587606013467 9 36.2771927242776 23.292149660925567 24.540022163229832 15.890635451567004 10-11 26.226442301187507 30.89152602122206 28.129527564381885 14.752504113208548 12-13 22.405092242072584 29.225614006538503 27.54388302826303 20.825410723125877 14-15 21.31733693539876 30.061118470229303 28.144771966906024 20.47677262746591 16-17 24.557240461035214 26.63799483376929 28.804942633784226 19.99982207141127 18-19 19.090792325272734 26.95814582100421 33.33484696195275 20.6162148917703 20-21 23.519462700144178 27.05819358370975 33.01117447139919 16.41116924474689 22-23 18.929340665226803 35.94289085397012 28.704190570414962 16.423577910388122 24-25 18.928840241070997 27.07816421437207 33.03579719328772 20.957198351269216 26-27 19.006702532869063 27.226400969859082 28.314239680558874 25.45265681671298 28-29 22.9049974394964 31.63397939327463 28.32589770996639 17.135125457262575 30-31 18.060752604568727 27.85644424913487 32.429394321816844 21.653408824479563 32-33 17.870267076371952 32.29282486285135 28.490027565957295 21.346880494819406 34-35 22.544423301009022 32.24945476934802 28.709380154252965 16.496741775389992 36-37 18.074273323889525 32.2786461784368 33.2317132502864 16.415367247387277 38-39 18.433559333531402 27.888462127992558 32.60175337503658 21.076225163439453 40-41 22.71027684020326 27.72289586933221 32.3787866122823 17.18804067818223 42-43 18.33458655604945 32.14678441338149 32.625959076795276 16.89266995377378 44-45 17.942087209844342 27.960745617164772 32.857136502550404 21.240030670440483 46-47 22.42700082412483 27.86208229738535 28.388834568130513 21.322082310359313 48-49 18.462442677475206 32.33364339062242 32.10053974434314 17.103374187559236 50-51 22.82272953938058 27.806744220722777 31.803884758173112 17.566641481723526 52-53 18.217992063061057 27.676214705486036 27.758444002617843 26.34734922883506 54-55 22.69581213127322 27.651690962380137 31.988570350307587 17.663926556039055 56-57 18.388888384276434 27.55338176747866 31.955701164054435 22.10202868419048 58-59 18.46432682629336 27.615201671279245 32.33182937973963 21.588642122687766 60-61 22.48887908600915 28.05682416352192 32.069064582854516 17.385232167614415 62-63 18.161997915224052 28.127404869123225 32.03264357300349 21.677953642649236 64-65 22.589869262115915 27.94140776293664 32.04235276104921 17.426370213898238 66-67 18.493928303794746 36.13471875740048 27.629255551606057 17.742097387198715 68-69 18.393672158688425 36.39892682089414 27.565378776348158 17.64202224406927 70-71 18.015126398750674 36.871134561474214 27.64293015673275 17.470808883042366 72-73 17.93176715761742 36.688077984628414 27.576054792113226 17.80410006564094 74-75 18.44687389523956 35.69526287991941 27.54890421667885 18.30895900816218 76-77 18.892304355623775 34.6618154989895 27.953977860175332 18.4919022852114 78-79 18.8928269597968 34.125487680339894 28.3406908418273 18.640994518036006 80-81 18.470980989598125 34.1002668931383 28.20240024962789 19.22635186763568 82-83 18.066981077084428 33.806215473414845 28.31776093273711 19.80904251676362 84-85 18.13526323993515 33.13950675839858 28.94030475116523 19.78492525050104 86-87 18.054802354405613 33.01265578751216 29.479443903519602 19.453097954562622 88-89 18.343192085365878 33.293481860473996 29.01403798176867 19.349288072391456 90-91 18.718142483834484 32.73309068178572 28.796353720073643 19.752413114306155 92-93 18.31964340152207 33.2322812790897 28.7135517672956 19.734523552092632 94-95 18.122397793291636 33.37984710239765 28.580729118391833 19.917025985918887 96-97 18.57719042974851 33.50301732871541 28.22543105821452 19.69436118332156 98-99 18.853952429812658 32.69837882713326 28.121489381484118 20.326179361569967 100-101 18.735375240081023 32.77836098138616 27.874748379516816 20.611515399016003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 463.0 1 336.0 2 302.0 3 653.0 4 763.0 5 536.0 6 460.5 7 421.5 8 390.5 9 378.5 10 369.5 11 394.0 12 410.0 13 405.5 14 434.0 15 496.0 16 604.0 17 820.0 18 1191.0 19 1785.0 20 2786.5 21 4366.0 22 6817.0 23 10449.0 24 15208.5 25 21369.5 26 29864.5 27 40549.5 28 52261.0 29 64494.0 30 77608.5 31 91463.0 32 107610.0 33 129559.5 34 158208.0 35 197714.5 36 255845.0 37 329277.5 38 395565.5 39 416858.5 40 414089.5 41 384808.0 42 331739.5 43 306017.5 44 278168.0 45 247071.5 46 239533.0 47 210734.0 48 148846.5 49 101258.0 50 74575.0 51 57490.5 52 45272.0 53 33868.5 54 25497.0 55 19504.5 56 14636.0 57 10987.0 58 8543.0 59 6570.0 60 4808.0 61 3505.5 62 2656.5 63 1996.5 64 1370.5 65 885.5 66 585.0 67 367.5 68 232.5 69 164.5 70 116.0 71 78.5 72 63.0 73 43.0 74 26.5 75 16.5 76 10.0 77 10.0 78 9.5 79 5.0 80 1.5 81 0.5 82 0.5 83 0.5 84 0.5 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19371975098152638 2 0.0 3 0.0 4 0.026596617169775197 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.633556998218676E-5 48-49 0.0034010308366925076 50-51 6.486979797506145E-5 52-53 0.002668928830973957 54-55 0.009396853592387474 56-57 0.013789465626698779 58-59 0.01395627367863465 60-61 0.0033917637226960704 62-63 0.0022055731311520894 64-65 0.010091887142120275 66-67 0.017153428007405536 68-69 0.025132413158338095 70-71 0.021221691051841532 72-73 0.017125626665416224 74-75 0.021388499103777405 76-77 0.01567068976797556 78-79 0.006811328787381453 80-81 0.00356783888862838 82-83 6.208966377613025E-4 84-85 0.0025206550070309593 86-87 0.015856032047904308 88-89 0.014984923332239197 90-91 0.018413755510921018 92-93 0.01869176893081414 94-95 0.01784846155713834 96-97 0.020054034688290426 98-99 0.02816275943517311 100-101 0.016495462913658485 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5395423.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.185783862994725 #Duplication Level Percentage of deduplicated Percentage of total 1 93.50695411389283 49.7324065118847 2 3.315648429229511 3.526907214453575 3 1.0126937967977918 1.6158274018764855 4 0.4696850932350716 0.9992227940988416 5 0.32956350801964346 0.8764046753331544 6 0.190803351329528 0.6088815482488394 7 0.09433243299116816 0.35120010746370917 8 0.11420439856678671 0.4859240366701138 9 0.08647431354972322 0.41392837351407735 >10 0.6775197844360118 7.138022601358158 >50 0.08634799377230218 3.2463042182583197 >100 0.09151192971608274 9.952637653866356 >500 0.016134777138136304 6.013631204685538 >1k 0.007879885338272939 6.393165657207516 >5k 2.1102204265544339E-4 0.7673033727714615 >10k+ 3.516994448327912E-5 7.878232628309105 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 421174 7.80613494067101 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 8438 0.15639181580387673 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 7549 0.13991488711821112 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6842 0.12681118792724871 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6611 0.12252978126089464 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 6021 0.11159458674509858 No Hit TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA 5559 0.10303177341239046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012603275035154796 0.0 0.0 2 0.0012788617315083544 0.0 0.0 3 0.0013529986434798532 0.0 0.0 4 0.0014642040114371015 0.0 0.0 5 0.001668080519358723 0.0 0.0 6 0.0018348885712945954 0.0 0.0 7 0.002112901991187716 0.0 5.5602683978624105E-5 8 0.0025206550070309593 0.0 5.5602683978624105E-5 9 0.0027059972869597063 0.0 5.5602683978624105E-5 10-11 0.005347124775944351 0.0 5.5602683978624105E-5 12-13 0.0074414925391391926 0.0 5.5602683978624105E-5 14-15 0.009600730100309096 0.0 9.267113996437351E-5 16-17 0.010768386463860202 0.0 1.1120536795724821E-4 18-19 0.012677411947126295 0.0 1.297395959501229E-4 20-21 0.014438163606449391 0.0 1.297395959501229E-4 22-23 0.016134045467797425 0.0 1.3900670994656026E-4 24-25 0.01808940652104571 0.0 1.482738239429976E-4 26-27 0.019961363548326054 0.0 1.482738239429976E-4 28-29 0.022194738021467455 0.0 1.5754093793943495E-4 30-31 0.038439988857222135 0.0 1.8534227992874703E-4 32-33 0.04109038346020322 0.0 1.8534227992874703E-4 34-35 0.043045744513451493 0.0 2.0387650792162172E-4 36-37 0.04540885858254302 0.0 2.4094496390737112E-4 38-39 0.048031451843534786 0.0 2.502120779038085E-4 40-41 0.050922791410423246 0.0 2.594791919002458E-4 42-43 0.05392533634526894 0.0 2.594791919002458E-4 44-45 0.05746537389190801 0.0 2.872805338895579E-4 46-47 0.06142243156838676 0.0 3.150818758788699E-4 48-49 0.06570383823474082 1.85342279928747E-5 3.243489898753073E-4 50-51 0.07055053885487755 1.85342279928747E-5 3.521503318646193E-4 52-53 0.07596253342879697 1.85342279928747E-5 3.521503318646193E-4 54-55 0.08247731456829242 1.85342279928747E-5 3.521503318646193E-4 56-57 0.08938131449563824 1.85342279928747E-5 3.614174458610567E-4 58-59 0.09655406072888076 1.85342279928747E-5 3.7995167385393143E-4 60-61 0.1052002780875568 1.85342279928747E-5 3.8921878785036875E-4 62-63 0.11461566590793715 1.85342279928747E-5 4.0775301584324345E-4 64-65 0.12571766847566912 1.85342279928747E-5 4.2628724383611815E-4 66-67 0.13804293009093077 1.85342279928747E-5 4.2628724383611815E-4 68-69 0.15207334068153694 1.85342279928747E-5 4.2628724383611815E-4 70-71 0.16767916065153743 1.85342279928747E-5 4.2628724383611815E-4 72-73 0.18486039000093227 1.85342279928747E-5 4.2628724383611815E-4 74-75 0.20446960321739371 1.85342279928747E-5 4.4482147182899284E-4 76-77 0.2256078902432673 1.85342279928747E-5 4.8188992781474224E-4 78-79 0.2517133503712313 1.85342279928747E-5 5.28225497796929E-4 80-81 0.2803858010762085 1.85342279928747E-5 5.374926117933664E-4 82-83 0.31302457657166083 1.85342279928747E-5 5.374926117933664E-4 84-85 0.3496760124275705 1.85342279928747E-5 5.467597257898037E-4 86-87 0.3901269650220196 1.85342279928747E-5 5.652939537826784E-4 88-89 0.4355636249465519 1.85342279928747E-5 5.745610677791158E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGGA 51915 0.0 88.28195 1 TCGGAAG 51810 0.0 88.19697 3 CGGAAGA 51800 0.0 87.975555 4 ATCGGAA 52085 0.0 87.74954 2 GAAGAGC 53140 0.0 85.88601 6 GGAAGAG 55460 0.0 82.413124 5 TCGGACG 155 0.0 73.555786 3 AGAGCAC 71645 0.0 63.775658 8 GAGCACA 71690 0.0 63.7025 9 AAGAGCA 73215 0.0 62.310772 7 ATCGGAC 215 0.0 55.23812 2 GCCGTCT 48670 0.0 46.406864 50-51 ATGCCGT 48630 0.0 46.281727 48-49 TCGTATG 48945 0.0 46.21991 44-45 TCTCGTA 48625 0.0 46.17244 42-43 CGTCTTC 48945 0.0 46.1721 52-53 TATGCCG 48560 0.0 46.16749 48-49 CGTATCT 48215 0.0 46.147484 38-39 TATCTCG 48325 0.0 46.125977 40-41 TGCCGTC 48705 0.0 46.11995 50-51 >>END_MODULE