FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_52370180-2.341000000358c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_52370180-2.341000000358c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5395423
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT4211747.80613494067101TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC84380.15639181580387673No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC75490.13991488711821112No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG68420.12681118792724871No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA66110.12252978126089464No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG60210.11159458674509858No Hit
TTGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGA55590.10303177341239046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA519150.088.281951
TCGGAAG518100.088.196973
CGGAAGA518000.087.9755554
ATCGGAA520850.087.749542
GAAGAGC531400.085.886016
GGAAGAG554600.082.4131245
TCGGACG1550.073.5557863
AGAGCAC716450.063.7756588
GAGCACA716900.063.70259
AAGAGCA732150.062.3107727
ATCGGAC2150.055.238122
GCCGTCT486700.046.40686450-51
ATGCCGT486300.046.28172748-49
TCGTATG489450.046.2199144-45
TCTCGTA486250.046.1724442-43
CGTCTTC489450.046.172152-53
TATGCCG485600.046.1674948-49
CGTATCT482150.046.14748438-39
TATCTCG483250.046.12597740-41
TGCCGTC487050.046.1199550-51
Produced by FastQC (version 0.11.2)