##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_52260024-1.34100000035820.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6664533 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.67694015469651 33.0 33.0 33.0 33.0 33.0 2 32.7100527523834 33.0 33.0 33.0 33.0 33.0 3 32.74183457415546 33.0 33.0 33.0 33.0 33.0 4 36.67008926206832 37.0 37.0 37.0 37.0 37.0 5 36.666016508583574 37.0 37.0 37.0 37.0 37.0 6 36.53037429629353 37.0 37.0 37.0 37.0 37.0 7 36.45202176956735 37.0 37.0 37.0 37.0 37.0 8 36.381932687556656 37.0 37.0 37.0 37.0 37.0 9 36.4116142871526 37.0 37.0 37.0 37.0 37.0 10-11 36.36172557026876 37.0 37.0 37.0 37.0 37.0 12-13 36.338614498570266 37.0 37.0 37.0 37.0 37.0 14-15 38.16307384178306 40.0 37.0 40.0 37.0 40.0 16-17 38.15128276804992 40.0 37.0 40.0 37.0 40.0 18-19 38.1482764058637 40.0 37.0 40.0 37.0 40.0 20-21 38.09431861167167 40.0 37.0 40.0 37.0 40.0 22-23 37.9546858722134 40.0 37.0 40.0 33.0 40.0 24-25 37.968814619118845 40.0 37.0 40.0 33.0 40.0 26-27 37.882918953210975 40.0 37.0 40.0 33.0 40.0 28-29 37.739450010976014 40.0 37.0 40.0 33.0 40.0 30-31 37.7025568033049 40.0 37.0 40.0 33.0 40.0 32-33 37.57813600742918 40.0 37.0 40.0 33.0 40.0 34-35 37.45586052316044 40.0 37.0 40.0 33.0 40.0 36-37 37.3077538216106 40.0 37.0 40.0 33.0 40.0 38-39 37.27294673160145 40.0 37.0 40.0 33.0 40.0 40-41 37.22061058141658 40.0 37.0 40.0 33.0 40.0 42-43 37.0132847267768 37.0 37.0 40.0 33.0 40.0 44-45 36.88629270798119 37.0 37.0 40.0 33.0 40.0 46-47 36.86614073334171 37.0 37.0 40.0 33.0 40.0 48-49 36.61346331393362 37.0 37.0 40.0 33.0 40.0 50-51 36.46878205869788 37.0 37.0 40.0 33.0 40.0 52-53 36.33211464329159 37.0 37.0 40.0 33.0 40.0 54-55 36.122556899335635 37.0 37.0 40.0 33.0 40.0 56-57 35.95556575381951 37.0 37.0 40.0 33.0 40.0 58-59 35.709784091398454 37.0 35.0 40.0 27.0 40.0 60-61 35.39152668311493 37.0 33.0 40.0 27.0 40.0 62-63 35.120383078604306 37.0 33.0 37.0 27.0 40.0 64-65 34.85574398086108 37.0 33.0 37.0 27.0 40.0 66-67 34.41539062076818 37.0 33.0 37.0 27.0 40.0 68-69 34.150485863000455 37.0 33.0 37.0 27.0 40.0 70-71 33.76806852032993 37.0 33.0 37.0 27.0 37.0 72-73 33.248813832867214 37.0 33.0 37.0 27.0 37.0 74-75 32.757424113587554 37.0 33.0 37.0 22.0 37.0 76-77 30.19011001971181 33.0 30.0 35.0 22.0 37.0 78-79 32.49663757385551 33.0 33.0 37.0 27.0 37.0 80-81 32.892696532525235 37.0 33.0 37.0 27.0 37.0 82-83 32.81255130704582 37.0 33.0 37.0 27.0 37.0 84-85 32.633021098402544 37.0 33.0 37.0 27.0 37.0 86-87 32.52527288858799 33.0 33.0 37.0 22.0 37.0 88-89 32.408447973774 33.0 33.0 37.0 22.0 37.0 90-91 32.26990758392223 33.0 33.0 37.0 22.0 37.0 92-93 32.13068912705511 33.0 33.0 37.0 22.0 37.0 94-95 32.00179322392132 33.0 33.0 37.0 22.0 37.0 96-97 31.82612225042625 33.0 33.0 37.0 22.0 37.0 98-99 31.64865985358614 33.0 33.0 37.0 22.0 37.0 100-101 29.929734386490395 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1102200344418236 2306 2 0.11892532726695038 2306 3 0.11356154502686167 2306 4 0.15167810248669156 2306 5 0.17041133373167128 2306 6 0.1647980711808401 2306 7 0.18847330207879764 2306 8 0.19245464668645695 2306 9 0.22531196844720114 2306 10-11 0.2738471358167729 2306 12-13 0.23573475824314016 2306 14-15 0.450344439701567 2306 16-17 0.46224515253583576 2306 18-19 0.37330506832459776 2306 20-21 0.5166945761933803 2306 22-23 0.5071897881893292 2306 24-25 0.4358295424979701 2306 26-27 0.5219148332444448 2306 28-29 0.4764569948952442 2306 30-31 0.43919054449613526 2306 32-33 0.5621422463212014 2306 34-35 0.5027974625000056 2306 36-37 0.5019525650308339 2306 38-39 0.5280549489456874 2306 40-41 0.49453306604185343 2306 42-43 0.5891163424344654 2306 44-45 0.5928655259130622 2306 46-47 0.4238600302440574 2306 48-49 0.4065677422433467 2306 50-51 0.40912352688742004 2306 52-53 0.3924554572663652 2306 54-55 0.4719356023103387 2306 56-57 0.5622636955676441 2306 58-59 0.5996747955547406 2306 60-61 0.5545320769287798 2306 62-63 0.5321916159917208 2306 64-65 0.447359688047797 2306 66-67 0.5536187396344232 2306 68-69 0.5675308677795812 2306 70-71 0.665601217603232 2306 72-73 0.3678182738388003 2306 74-75 0.38590837826811253 2306 76-77 0.47732920879866825 2306 78-79 0.5775569897760207 2306 80-81 0.5169725153338618 2306 82-83 0.44072926317423367 2306 84-85 0.5382893822065355 2306 86-87 0.5280276521125273 2306 88-89 0.5303332722095675 2306 90-91 0.5027643059914197 2306 92-93 0.5817570724609951 2306 94-95 0.4379724044426965 2306 96-97 0.642145949636646 2306 98-99 0.652432012506889 2306 100-101 0.49078619796347667 2307 1 0.07959690018091692 2307 2 0.06339480380250961 2307 3 0.10062005010357922 2307 4 0.16082646726349026 2307 5 0.14847676649496577 2307 6 0.17192566107119944 2307 7 0.189214776282995 2307 8 0.2158816642816106 2307 9 0.1993005814874138 2307 10-11 0.17320735260940268 2307 12-13 0.1533782971767934 2307 14-15 0.3971449338190709 2307 16-17 0.4291157782919939 2307 18-19 0.3792102484455313 2307 20-21 0.42373448241074385 2307 22-23 0.4252893603318242 2307 24-25 0.4865218823600159 2307 26-27 0.434862134020257 2307 28-29 0.44779901287671464 2307 30-31 0.38616769033338727 2307 32-33 0.47429569138002137 2307 34-35 0.5114233408113478 2307 36-37 0.42800359847460356 2307 38-39 0.4500411206289314 2307 40-41 0.42837714797974513 2307 42-43 0.4738218949191051 2307 44-45 0.5322542372823449 2307 46-47 0.40862755879530255 2307 48-49 0.3790732659564 2307 50-51 0.35549752034001614 2307 52-53 0.370767799812306 2307 54-55 0.3216231673216896 2307 56-57 0.4030268760909621 2307 58-59 0.37072051777078485 2307 60-61 0.40052753040673394 2307 62-63 0.3995483389656229 2307 64-65 0.27855563287319285 2307 66-67 0.4052667663068803 2307 68-69 0.3999837273370943 2307 70-71 0.285285103024691 2307 72-73 0.18685357467033015 2307 74-75 0.1551067965799504 2307 76-77 0.2725142526326536 2307 78-79 0.31220794832348275 2307 80-81 0.23981031539567965 2307 82-83 0.20568771811206688 2307 84-85 0.2402873725440955 2307 86-87 0.22608668152778932 2307 88-89 0.14264062470175531 2307 90-91 0.10628968245888615 2307 92-93 0.268055465822151 2307 94-95 0.03309521855633335 2307 96-97 0.296741187299542 2307 98-99 0.36066990526103737 2307 100-101 0.290299883328224 2201 1 0.10257527606658812 2201 2 0.09956136219926748 2201 3 0.09268466800389774 2201 4 0.1621152024426351 2201 5 0.16169821681675955 2201 6 0.15658890130288228 2201 7 0.2065235322548915 2201 8 0.2569578787052862 2201 9 0.2123583840842258 2201 10-11 0.20561954511997982 2201 12-13 0.24157049705637945 2201 14-15 0.4642702551945419 2201 16-17 0.4224830926380747 2201 18-19 0.4133892420508971 2201 20-21 0.42611494378444803 2201 22-23 0.4678329281744382 2201 24-25 0.40404139178166787 2201 26-27 0.4324527034855592 2201 28-29 0.5119667698117709 2201 30-31 0.4860857094767326 2201 32-33 0.481723627227872 2201 34-35 0.4870214764234291 2201 36-37 0.5621706792133665 2201 38-39 0.5315925982486434 2201 40-41 0.5032687089342005 2201 42-43 0.5014338413824575 2201 44-45 0.5972047596374352 2201 46-47 0.5587216039057807 2201 48-49 0.5398510441951956 2201 50-51 0.552186965738322 2201 52-53 0.5508767365667211 2201 54-55 0.5436804771437309 2201 56-57 0.6478658518505824 2201 58-59 0.5937024858324165 2201 60-61 0.5784081406071593 2201 62-63 0.5036728298045858 2201 64-65 0.5382200346747794 2201 66-67 0.6321990439217515 2201 68-69 0.6176187171793899 2201 70-71 0.4083789742828259 2201 72-73 0.47764614362824176 2201 74-75 0.5897141861382025 2201 76-77 0.3495594744648436 2201 78-79 0.5646655143899011 2201 80-81 0.560076183515271 2201 82-83 0.501595773658245 2201 84-85 0.5840602943927351 2201 86-87 0.6966512104834308 2201 88-89 0.5741065049921446 2201 90-91 0.6968924529498253 2201 92-93 0.7390211896229957 2201 94-95 0.6092330701966304 2201 96-97 0.6953552914233789 2201 98-99 0.714631131515528 2201 100-101 0.7660506210045455 1101 1 0.048140659405035535 1101 2 0.060806260473384555 1101 3 0.10141419185467981 1101 4 0.12429000307751892 1101 5 0.12511993586909398 1101 6 0.14200014058793897 1101 7 0.13871829720262951 1101 8 0.12625412598451646 1101 9 0.14790239886384882 1101 10-11 0.19989885094481252 1101 12-13 0.19317779925864187 1101 14-15 0.3671558164356199 1101 16-17 0.28379284490397083 1101 18-19 0.3056693758470743 1101 20-21 0.3146361704644747 1101 22-23 0.2802954161759885 1101 24-25 0.31434978219585474 1101 26-27 0.30004419820308925 1101 28-29 0.24027969795238846 1101 30-31 0.33214789615391993 1101 32-33 0.37388742485645565 1101 34-35 0.3394792781304119 1101 36-37 0.37661685284667357 1101 38-39 0.35125561069364153 1101 40-41 0.42681964530520133 1101 42-43 0.4536036460632644 1101 44-45 0.34441925263296014 1101 46-47 0.39324445744446734 1101 48-49 0.39042751898964667 1101 50-51 0.38838812246883236 1101 52-53 0.3666510757439241 1101 54-55 0.4965669468334184 1101 56-57 0.4309755614074149 1101 58-59 0.40516487628267583 1101 60-61 0.34055176314912217 1101 62-63 0.40705779826157595 1101 64-65 0.3191561569145307 1101 66-67 0.43572806050578095 1101 68-69 0.44132470228856135 1101 70-71 0.41784692484912256 1101 72-73 0.24424195130389137 1101 74-75 0.33688775617594047 1101 76-77 0.1499024369085511 1101 78-79 0.4665926024761191 1101 80-81 0.5636096550901897 1101 82-83 0.4310147234307564 1101 84-85 0.42713688441131836 1101 86-87 0.5641507825203149 1101 88-89 0.3402434520279485 1101 90-91 0.5561452106901328 1101 92-93 0.7225436256859226 1101 94-95 0.6219251749981325 1101 96-97 0.6525258233763189 1101 98-99 0.6732106484323275 1101 100-101 0.49531651344032923 2304 1 0.07849236098666523 2304 2 0.06616960343534828 2304 3 0.07296766815186828 2304 4 0.1094954601022593 2304 5 0.12823229383150192 2304 6 0.13763250684521466 2304 7 0.12281501855621713 2304 8 0.10052358536416506 2304 9 0.07436448535780471 2304 10-11 0.13110815794089348 2304 12-13 0.13562590026247534 2304 14-15 0.24437936232387614 2304 16-17 0.3053639057013413 2304 18-19 0.2855159020720066 2304 20-21 0.25530230556469036 2304 22-23 0.28659093009290615 2304 24-25 0.25061547396452255 2304 26-27 0.23640400045186283 2304 28-29 0.3054032427172615 2304 30-31 0.24455820057402633 2304 32-33 0.21115015888058508 2304 34-35 0.23594842502259183 2304 36-37 0.25227314241043075 2304 38-39 0.20167580342627645 2304 40-41 0.2550260097020356 2304 42-43 0.25675105724589997 2304 44-45 0.3139439166350755 2304 46-47 0.2985628996157743 2304 48-49 0.32383331608883026 2304 50-51 0.3678926748344864 2304 52-53 0.3416304551442195 2304 54-55 0.2421284446917582 2304 56-57 0.24918089974186586 2304 58-59 0.2560898704334278 2304 60-61 0.2834727321563122 2304 62-63 0.27003052975387476 2304 64-65 0.2087224917542727 2304 66-67 0.28248424983835463 2304 68-69 0.29498717707782873 2304 70-71 0.27609002190408205 2304 72-73 0.15794511311249693 2304 74-75 0.17184729461496318 2304 76-77 0.07872920763048441 2304 78-79 0.3717589032827533 2304 80-81 0.2407995840578252 2304 82-83 0.34664646948197486 2304 84-85 0.286452246392777 2304 86-87 0.31140805266051075 2304 88-89 0.22131107072863898 2304 90-91 0.2952285279889537 2304 92-93 0.384059958774877 2304 94-95 0.2568189127663132 2304 96-97 0.36677051394153537 2304 98-99 0.4776374301815416 2304 100-101 0.35836267144686573 2202 1 0.03948733706005214 2202 2 0.04982027278180823 2202 3 0.1041263777921273 2202 4 0.11381451829542755 2202 5 0.11226060901284285 2202 6 0.09541787899562593 2202 7 0.13323317554169734 2202 8 0.10825551172987957 2202 9 0.17808670372011903 2202 10-11 0.13117052278374075 2202 12-13 0.12816575948191655 2202 14-15 0.13946158449835622 2202 16-17 0.06949592764554069 2202 18-19 0.11576437069086865 2202 20-21 0.07815832625462349 2202 22-23 0.07335756182230568 2202 24-25 0.08251621096098205 2202 26-27 0.12188955032065252 2202 28-29 0.1692064822380246 2202 30-31 0.06100218408936797 2202 32-33 0.21090207079296874 2202 34-35 0.22907569364016922 2202 36-37 0.20373851052470116 2202 38-39 0.21963433557670697 2202 40-41 0.17048843686973214 2202 42-43 0.22981734430312173 2202 44-45 0.19652704442820834 2202 46-47 0.30374405248603864 2202 48-49 0.30761287182489383 2202 50-51 0.15184987466846422 2202 52-53 0.3196124231055464 2202 54-55 0.3517111441872558 2202 56-57 0.2793825533043943 2202 58-59 0.23072126364402123 2202 60-61 0.1467648827909187 2202 62-63 0.22518496544547162 2202 64-65 0.16112374675316943 2202 66-67 0.20164107259216024 2202 68-69 0.19450691636479434 2202 70-71 0.18347554485000472 2202 72-73 0.2728146257490991 2202 74-75 0.32326774747228626 2202 76-77 0.2375666058298016 2202 78-79 0.25790494054692914 2202 80-81 0.40623637107596977 2202 82-83 0.30495690047188617 2202 84-85 0.17180815280905648 2202 86-87 0.18679304057721424 2202 88-89 0.24784384029674555 2202 90-91 0.16756435218306365 2202 92-93 0.29001968942731793 2202 94-95 0.0865616347920124 2202 96-97 0.22778660492803837 2202 98-99 0.3528217107215106 2202 100-101 0.528711239780904 1102 1 0.029208084019103353 1102 2 0.04867730719198704 1102 3 0.03035722972953181 1102 4 0.09074469490784765 1102 5 0.08416208515746604 1102 6 0.055927782271062654 1102 7 0.06707240481714649 1102 8 0.08182578409633123 1102 9 0.06488068022974858 1102 10-11 0.09194210183060392 1102 12-13 0.06361462969242382 1102 14-15 0.05970000324879976 1102 16-17 0.07391835895050747 1102 18-19 0.06292406243142779 1102 20-21 0.042174505528841166 1102 22-23 0.1279924668911221 1102 24-25 0.0970929122912878 1102 26-27 0.06848158339317933 1102 28-29 0.0762732366187393 1102 30-31 0.09507346665827043 1102 32-33 0.189938681981495 1102 34-35 0.08362378839159135 1102 36-37 0.08159544978005329 1102 38-39 0.07485790300075479 1102 40-41 0.0885102070701933 1102 42-43 0.18396250267367265 1102 44-45 0.20739613607307206 1102 46-47 0.2513109214323066 1102 48-49 0.18704841065439126 1102 50-51 0.09718387549215635 1102 52-53 0.13350553933958764 1102 54-55 0.18866443118518816 1102 56-57 0.13373872963325084 1102 58-59 0.10247212721098009 1102 60-61 0.02663539627252476 1102 62-63 0.12974923757223422 1102 64-65 0.10360128304070315 1102 66-67 0.14944337588750756 1102 68-69 0.09599585203168459 1102 70-71 0.006108900857334731 1102 72-73 0.009835618254022904 1102 74-75 0.011861790611391143 1102 76-77 0.06989747287382642 1102 78-79 0.24321372639667516 1102 80-81 0.26263281139933525 1102 82-83 0.16898492662743791 1102 84-85 0.16825931032214925 1102 86-87 0.05722265512210356 1102 88-89 0.04866363815497721 1102 90-91 -0.06890182544546519 1102 92-93 0.05282260247310688 1102 94-95 -0.2162261906227272 1102 96-97 0.025162974334687505 1102 98-99 0.09008138149003742 1102 100-101 0.10309870959931544 2305 1 0.028583837142207358 2305 2 0.04445214762042582 2305 3 0.056448506380661456 2305 4 0.02756507579407952 2305 5 0.0745091477795441 2305 6 -0.0015575441165083248 2305 7 -0.019706309446917203 2305 8 -0.009162506626495315 2305 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1213 88-89 -0.1476738337587591 1213 90-91 -0.1577510718334736 1213 92-93 -0.23949764953428243 1213 94-95 -0.1436845487584435 1213 96-97 -0.24404897474146026 1213 98-99 -0.06307182853295501 1213 100-101 0.020237038636189197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 10.0 8 96.0 9 289.0 10 198.0 11 94.0 12 103.0 13 217.0 14 471.0 15 1246.0 16 2401.0 17 3878.0 18 6098.0 19 8567.0 20 12010.0 21 16057.0 22 21262.0 23 27771.0 24 35632.0 25 46477.0 26 60656.0 27 83465.0 28 124306.0 29 168028.0 30 158368.0 31 166658.0 32 220780.0 33 313410.0 34 479400.0 35 827708.0 36 1525507.0 37 1980222.0 38 372807.0 39 340.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.45664777513459 25.84167345588148 21.823816380141505 15.877862388842425 2 29.59902816896548 24.983806067131788 31.129097867772582 14.288067896130158 3 35.593176596169606 19.492243492529784 27.37644183020776 17.538138081092853 4 28.71500934284878 19.325258710611827 31.7610031743173 20.198728772222097 5 33.748336154986404 17.99245348473779 30.78169168042232 17.47751867985349 6 28.85165397185369 19.034912123625165 34.72591402878491 17.387519875736228 7 31.839860347304157 21.89164642143718 27.47107711823169 18.797416113026973 8 28.495199888724386 27.81878340162769 25.224122980559926 18.461893729087993 9 31.000536721777806 23.374105882587724 25.9825707217595 19.642786673874973 10-11 24.945791400537743 28.4437034072755 29.152852870561226 17.457652321625535 12-13 23.70844288714603 27.056959579913553 28.61701637609117 20.617581156849248 14-15 23.372845479195618 27.49230141106661 28.629455357187066 20.505397752550703 16-17 24.31660252863929 26.333142922392312 29.301355398795387 20.048899150173014 18-19 21.968080884287016 26.378374898886385 31.007311390010372 20.64623282681622 20-21 23.38733261580369 26.718503757127472 30.519595296474638 19.374568330594208 22-23 21.944140722238153 29.854845043156065 28.70014298076099 19.500871253844792 24-25 21.83562599209877 26.26541124486892 30.692578159639993 21.206384603392316 26-27 21.71200142605641 26.375914111311328 29.173454464101233 22.738629998531028 28-29 22.83142719827481 28.419433139576324 28.68281243411954 20.066327228029333 30-31 20.883713832612127 27.510487231438425 30.162375968428695 21.443422967520757 32-33 20.694203179727673 28.952921382488466 28.98257087180752 21.37030456597634 34-35 22.497495323378246 29.137705522652524 29.130503217554775 19.23429593641445 36-37 22.217835818353667 29.44200291303232 29.44459124142682 18.8955700271872 38-39 21.02914037637746 27.610719310715393 31.95737795881572 19.402762354091426 40-41 21.012725122675512 27.26623905981109 28.738960404277393 22.982075413236007 42-43 21.29613582827184 28.93448798287892 30.180734344026806 19.588641844822437 44-45 20.889940825561222 27.547218987436928 30.446761986173676 21.116078200828177 46-47 22.471819830574624 27.472632341349957 29.00443647388629 21.05111135418913 48-49 21.09004609551365 29.257607815477492 30.001742864926317 19.650603224082534 50-51 22.449745296945746 27.830638505321946 29.42124686896314 20.298369328769166 52-53 20.787789725171432 27.48822860488589 28.390739118094633 23.333242551848045 54-55 22.520900497179092 27.58262376974192 29.79060374061932 20.10587199245967 56-57 21.21470671633845 27.300553446379688 29.472057069379147 22.012682767902707 58-59 21.16425377196288 27.429856324827533 29.624369688754065 21.78152021445552 60-61 22.35007922058766 27.68244481080767 29.490540871265907 20.47693509733876 62-63 20.835322768693814 27.73346918013256 29.50113063950531 21.930077411668318 64-65 22.6744033365702 27.645696953817534 29.443419261150726 20.236480448461542 66-67 21.71552442617842 30.392105440284865 27.734277539747698 20.158092593789018 68-69 21.771599905295748 30.334793044565405 27.721368378910068 20.172238671228783 70-71 21.432952695205795 30.884921738485744 27.81912317508238 19.86300239122608 72-73 21.387666705585772 31.165829305606124 27.487733728487314 19.95877026032079 74-75 21.95998129394737 30.440513732079623 27.26439438554279 20.335110588430222 76-77 22.026579145028247 29.53346554623362 27.808184743891907 20.631770564846224 78-79 21.785759999831935 29.270641274641225 28.45844449509738 20.485154230429465 80-81 21.36362248371003 29.545231341233784 28.266327426261768 20.824818748794417 82-83 20.466755860781856 29.608414926842496 28.569738376849664 21.355090835525985 84-85 20.66227429262268 29.550683348354795 28.517537372529834 21.269504986492688 86-87 20.47755391833398 29.674476776844212 29.045501456676316 20.80246784814549 88-89 20.595402047352973 29.95405138807038 28.87436105572256 20.57618550885409 90-91 21.000159980694264 29.406680229507764 28.3989744427089 21.19418534708907 92-93 20.603884046639177 29.948084896595635 28.077295236994683 21.370735819770513 94-95 20.3779032903144 30.11859659075134 28.01364029627762 21.489859822656637 96-97 20.72885007275399 30.56020984485177 27.65906244716791 21.051877635226333 98-99 20.94027552235757 30.01344734496549 27.638740330710583 21.407536801966366 100-101 21.06216555766483 29.881309527856136 27.255497529836198 21.801027384642836 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 21.0 1 14.5 2 22.0 3 59.5 4 78.0 5 69.5 6 62.5 7 56.0 8 56.0 9 60.0 10 63.0 11 67.0 12 62.5 13 60.5 14 62.5 15 77.0 16 93.5 17 111.5 18 161.0 19 203.0 20 301.0 21 478.0 22 726.0 23 1064.0 24 1539.5 25 2287.0 26 3651.5 27 5857.5 28 8280.5 29 10647.0 30 13675.0 31 17872.5 32 29452.0 33 52771.5 34 90008.5 35 144115.0 36 220757.5 37 317442.0 38 407745.5 39 444086.0 40 453162.0 41 442311.5 42 418527.0 43 427328.0 44 425342.5 45 410665.0 46 409179.0 47 371051.0 48 297146.5 49 243672.0 50 202614.0 51 172117.0 52 150611.5 53 122413.0 54 89629.0 55 61461.5 56 43568.0 57 32427.5 58 26162.0 59 20890.0 60 16120.5 61 12731.5 62 9746.0 63 7418.0 64 5978.0 65 4718.5 66 3468.5 67 2593.5 68 1993.5 69 1497.0 70 1058.5 71 731.5 72 560.5 73 410.5 74 291.0 75 216.0 76 148.5 77 110.0 78 79.5 79 54.5 80 37.0 81 21.5 82 15.0 83 16.5 84 13.5 85 6.0 86 3.0 87 3.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19584267944955783 2 0.0 3 0.0 4 0.025253082248973782 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.002881372183167E-5 48-49 0.0032785492997033697 50-51 1.1253601715228959E-4 52-53 0.0026783572082244925 54-55 0.009550556655657643 56-57 0.013871939714305562 58-59 0.014404610195493068 60-61 0.0034511045260035476 62-63 0.002445782772776427 64-65 0.01026328476428881 66-67 0.017398068251743972 68-69 0.025290594254691216 70-71 0.02136683845664805 72-73 0.017090469804861046 74-75 0.021741958513822348 76-77 0.015574984773876878 78-79 0.006692141819989487 80-81 0.0034886165317209775 82-83 6.377040971963078E-4 84-85 0.002693362010511464 86-87 0.016145167260781815 88-89 0.015034811891545891 90-91 0.01844090201068852 92-93 0.018763505259858416 94-95 0.01782570511692267 96-97 0.019873860629094343 98-99 0.02820152589836377 100-101 0.0166328233351084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6664533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.67506952550304 #Duplication Level Percentage of deduplicated Percentage of total 1 84.67882694168276 26.822077307158416 2 6.447998287639689 4.084815881226234 3 2.456607530001111 2.3343964292897845 4 1.2455864161222427 1.5781614532277684 5 0.8987864249596859 1.4234561249588185 6 0.6401122684848494 1.2165360365031042 7 0.4505160360429801 0.9989088734810799 8 0.2712104720893573 0.6872488447579916 9 0.2814596155733741 0.8023727602717184 >10 2.0538777946360796 12.79394517947951 >50 0.25714389559126427 5.682800921607954 >100 0.249314126079695 17.442716537852874 >500 0.0469941824379959 10.317128469365246 >1k 0.021280536540444882 10.705669123315447 >5k 2.3790232436257368E-4 0.44557418790974435 >10k+ 4.75697942113607E-5 2.664191869594475 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 176814 2.6530591115686573 TruSeq Adapter, Index 7 (97% over 38bp) GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 6747 0.10123740103019972 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001635523449279942 0.0 0.0 2 0.0018305858790105772 0.0 0.0 3 0.0020706627156021284 0.0 0.0 4 0.0022807299476197357 0.0 0.0 5 0.0025958307956461465 0.0 0.0 6 0.003075984468829249 0.0 0.0 7 0.0038112197808908743 0.0 0.0 8 0.0044414214769436956 0.0 0.0 9 0.004951584754700742 0.0 0.0 10-11 0.007442381934338085 0.0 0.0 12-13 0.01007572473570166 0.0 0.0 14-15 0.012919134769082845 0.0 0.0 16-17 0.015732535197890085 0.0 0.0 18-19 0.018966070090732538 7.502401143485972E-6 0.0 20-21 0.022829806679627813 1.5004802286971945E-5 0.0 22-23 0.02649097843764897 1.5004802286971945E-5 0.0 24-25 0.030707327880288085 1.5004802286971945E-5 0.0 26-27 0.0349236773229272 1.5004802286971945E-5 0.0 28-29 0.03965019004332336 1.5004802286971945E-5 0.0 30-31 0.06735655746621706 1.5004802286971945E-5 0.0 32-33 0.0742812737216546 1.5004802286971945E-5 0.0 34-35 0.07998309859070396 1.5004802286971945E-5 0.0 36-37 0.08640515396952794 1.5004802286971945E-5 0.0 38-39 0.0934874206489787 1.5004802286971945E-5 0.0 40-41 0.10127491303591715 1.5004802286971945E-5 0.0 42-43 0.1105028664424049 1.5004802286971945E-5 0.0 44-45 0.1207586488055502 1.5004802286971945E-5 0.0 46-47 0.13192222170705736 1.5004802286971945E-5 0.0 48-49 0.14376851311262168 1.5004802286971945E-5 0.0 50-51 0.15716780155488763 1.5004802286971945E-5 0.0 52-53 0.1717374645755374 1.5004802286971945E-5 0.0 54-55 0.1878901342374627 1.5004802286971945E-5 0.0 56-57 0.2060234378012683 1.5004802286971945E-5 0.0 58-59 0.22585228402350171 1.5004802286971945E-5 0.0 60-61 0.24766926654875893 1.5004802286971945E-5 0.0 62-63 0.2721270942765232 1.5004802286971945E-5 0.0 64-65 0.2996233944673993 1.5004802286971945E-5 0.0 66-67 0.32913784056587314 1.5004802286971945E-5 0.0 68-69 0.36328877057102127 1.5004802286971945E-5 0.0 70-71 0.4001630721912548 1.5004802286971945E-5 0.0 72-73 0.4408635983946662 1.5004802286971945E-5 0.0 74-75 0.4860580628830257 1.5004802286971945E-5 0.0 76-77 0.5363091457420948 1.5004802286971945E-5 0.0 78-79 0.5914292869432862 1.5004802286971945E-5 0.0 80-81 0.6547420501931642 1.5004802286971945E-5 0.0 82-83 0.7238541695269571 1.5004802286971945E-5 0.0 84-85 0.8028244439632904 1.5004802286971945E-5 1.5004802286971945E-5 86-87 0.8890795499099486 1.5004802286971945E-5 1.5004802286971945E-5 88-89 0.9862131375146616 1.5004802286971945E-5 1.5004802286971945E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 28285 0.0 66.89503 4 TCGGAAG 28400 0.0 66.674324 3 GAAGAGC 28600 0.0 66.22291 6 GATCGGA 29020 0.0 65.326256 1 ATCGGAA 29445 0.0 64.340324 2 GGAAGAG 31635 0.0 59.974693 5 CGACGCG 60 1.4726411E-6 47.59648 1 CGCGTTG 40 5.3946127E-4 47.491856 5 TTGTGTT 61970 0.0 45.207825 1 GCCGTCT 21165 0.0 44.414005 50-51 ATGCCGT 21060 0.0 44.339573 48-49 TGCCGTC 21170 0.0 44.044518 50-51 TCGTATG 21430 0.0 43.960083 44-45 CGTCTTC 21375 0.0 43.9584 52-53 TATGCCG 21165 0.0 43.91763 48-49 CGTATGC 21285 0.0 43.7693 46-47 TCTCGTA 21295 0.0 43.41354 42-43 CATCTCG 21225 0.0 43.262897 40-41 ATCTCGT 21165 0.0 43.152847 42-43 CTCGTAT 21355 0.0 43.147 44-45 >>END_MODULE