FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_52260024-1.34100000035820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_52260024-1.34100000035820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6664533
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT1768142.6530591115686573TruSeq Adapter, Index 7 (97% over 38bp)
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC67470.10123740103019972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA282850.066.895034
TCGGAAG284000.066.6743243
GAAGAGC286000.066.222916
GATCGGA290200.065.3262561
ATCGGAA294450.064.3403242
GGAAGAG316350.059.9746935
CGACGCG601.4726411E-647.596481
CGCGTTG405.3946127E-447.4918565
TTGTGTT619700.045.2078251
GCCGTCT211650.044.41400550-51
ATGCCGT210600.044.33957348-49
TGCCGTC211700.044.04451850-51
TCGTATG214300.043.96008344-45
CGTCTTC213750.043.958452-53
TATGCCG211650.043.9176348-49
CGTATGC212850.043.769346-47
TCTCGTA212950.043.4135442-43
CATCTCG212250.043.26289740-41
ATCTCGT211650.043.15284742-43
CTCGTAT213550.043.14744-45
Produced by FastQC (version 0.11.2)