##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_52150223-1.3410000003573e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3271564 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.694545483444614 33.0 33.0 33.0 33.0 33.0 2 32.72403474301588 33.0 33.0 33.0 33.0 33.0 3 32.75712747786685 33.0 33.0 33.0 33.0 33.0 4 36.68288775643698 37.0 37.0 37.0 37.0 37.0 5 36.67253399291592 37.0 37.0 37.0 37.0 37.0 6 36.54353208434865 37.0 37.0 37.0 37.0 37.0 7 36.460961485087864 37.0 37.0 37.0 37.0 37.0 8 36.39997200115908 37.0 37.0 37.0 37.0 37.0 9 36.426455664630126 37.0 37.0 37.0 37.0 37.0 10-11 36.36798301974224 37.0 37.0 37.0 37.0 37.0 12-13 36.33840099108561 37.0 37.0 37.0 37.0 37.0 14-15 38.152235291744255 40.0 37.0 40.0 37.0 40.0 16-17 38.15385775732952 40.0 37.0 40.0 37.0 40.0 18-19 38.132525605490216 40.0 37.0 40.0 37.0 40.0 20-21 38.08359411584184 40.0 37.0 40.0 37.0 40.0 22-23 37.92459279414983 40.0 37.0 40.0 33.0 40.0 24-25 37.93878508872209 40.0 37.0 40.0 33.0 40.0 26-27 37.86231310162357 40.0 37.0 40.0 33.0 40.0 28-29 37.67583669462068 40.0 37.0 40.0 33.0 40.0 30-31 37.62834824567088 40.0 37.0 40.0 33.0 40.0 32-33 37.50798425462561 40.0 37.0 40.0 33.0 40.0 34-35 37.442632025538856 40.0 37.0 40.0 33.0 40.0 36-37 37.35717687320194 40.0 37.0 40.0 33.0 40.0 38-39 37.177727380543374 40.0 37.0 40.0 33.0 40.0 40-41 37.0569313331483 40.0 37.0 40.0 33.0 40.0 42-43 36.88816281753925 37.0 37.0 40.0 33.0 40.0 44-45 36.71144474019154 37.0 37.0 40.0 33.0 40.0 46-47 36.72335830813641 37.0 37.0 40.0 33.0 40.0 48-49 36.42208665335601 37.0 37.0 40.0 33.0 40.0 50-51 36.339255475362854 37.0 37.0 40.0 33.0 40.0 52-53 36.264735307027465 37.0 37.0 40.0 33.0 40.0 54-55 35.9581985863642 37.0 37.0 40.0 30.0 40.0 56-57 35.81231988736886 37.0 37.0 40.0 27.0 40.0 58-59 35.639010882868256 37.0 37.0 40.0 27.0 40.0 60-61 35.36493585331053 37.0 33.0 40.0 27.0 40.0 62-63 35.138578368022145 37.0 33.0 40.0 27.0 40.0 64-65 34.94901994886849 37.0 33.0 40.0 27.0 40.0 66-67 34.47231859135264 37.0 33.0 38.5 27.0 40.0 68-69 34.330949050668124 37.0 33.0 37.0 27.0 40.0 70-71 34.020345467794606 37.0 33.0 37.0 27.0 40.0 72-73 33.486349036729834 37.0 33.0 37.0 24.5 40.0 74-75 32.91242690040605 37.0 33.0 37.0 22.0 40.0 76-77 30.41882735596797 33.0 30.0 35.0 22.0 37.0 78-79 32.51041000573426 35.0 33.0 37.0 22.0 37.0 80-81 32.75586737719329 37.0 33.0 37.0 22.0 37.0 82-83 32.58256356898413 37.0 33.0 37.0 22.0 37.0 84-85 32.35097892017396 37.0 33.0 37.0 22.0 37.0 86-87 32.199522002320606 37.0 33.0 37.0 22.0 37.0 88-89 32.01451385331298 35.0 33.0 37.0 22.0 37.0 90-91 31.83027460260597 33.0 33.0 37.0 18.5 37.0 92-93 31.679254478897555 33.0 33.0 37.0 15.0 37.0 94-95 31.53912165557513 33.0 33.0 37.0 15.0 37.0 96-97 31.358729341684892 33.0 33.0 37.0 15.0 37.0 98-99 31.171969431134467 33.0 33.0 37.0 15.0 37.0 100-101 29.49693617486927 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.08049478497184737 2306 2 0.08332971907321962 2306 3 0.12334997249036661 2306 4 0.13820268626208332 2306 5 0.11203113905070694 2306 6 0.24249056929710378 2306 7 0.24589651775522015 2306 8 0.24140904525253148 2306 9 0.18997241982533808 2306 10-11 0.2191723634994176 2306 12-13 0.24184576210893738 2306 14-15 0.44386306327763236 2306 16-17 0.49187618049317905 2306 18-19 0.3874638067259255 2306 20-21 0.4651276684255663 2306 22-23 0.44572798859537244 2306 24-25 0.42374243953769053 2306 26-27 0.5480325836402713 2306 28-29 0.5348054867266896 2306 30-31 0.5456522247751252 2306 32-33 0.6884334771782861 2306 34-35 0.6342284572699199 2306 36-37 0.5879870007305215 2306 38-39 0.549008918706889 2306 40-41 0.5481506088744794 2306 42-43 0.5770819532149858 2306 44-45 0.6723060726974168 2306 46-47 0.5702740834991289 2306 48-49 0.4431797883870843 2306 50-51 0.5567951875262125 2306 52-53 0.5211786806837608 2306 54-55 0.3736775496329656 2306 56-57 0.5278437393295476 2306 58-59 0.5806292909670034 2306 60-61 0.34158703382161804 2306 62-63 0.35188644444062334 2306 64-65 0.41435128186380155 2306 66-67 0.440857525575737 2306 68-69 0.6763954326277215 2306 70-71 0.6945107493256586 2306 72-73 0.5709634572101407 2306 74-75 0.5520857009943612 2306 76-77 0.45028699182513066 2306 78-79 0.534597006323061 2306 80-81 0.5948000198580132 2306 82-83 0.5561480514792194 2306 84-85 0.5829790300410949 2306 86-87 0.5373574031579054 2306 88-89 0.5423596515014637 2306 90-91 0.5350528759303081 2306 92-93 0.5742902214823467 2306 94-95 0.4764475414534104 2306 96-97 0.4453457300120576 2306 98-99 0.5971618012766058 2306 100-101 0.45926949386371163 2307 1 0.06402527354087084 2307 2 0.10233449913659598 2307 3 0.08914946942002899 2307 4 0.17813411831178172 2307 5 0.1273604621435993 2307 6 0.14989026852907017 2307 7 0.08494802428081272 2307 8 0.18117196664108093 2307 9 0.18726170317388835 2307 10-11 0.243122248921118 2307 12-13 0.20900151250727816 2307 14-15 0.4337974045419344 2307 16-17 0.49576792637999745 2307 18-19 0.40617028039977043 2307 20-21 0.40425045070889354 2307 22-23 0.4733080143754975 2307 24-25 0.3978745984434653 2307 26-27 0.30203633428924803 2307 28-29 0.2974396462769704 2307 30-31 0.3741508910121212 2307 32-33 0.5256157014295013 2307 34-35 0.4596198585267004 2307 36-37 0.5348245725657463 2307 38-39 0.5407964296723904 2307 40-41 0.3496139438190724 2307 42-43 0.4447765796718315 2307 44-45 0.4641025350924579 2307 46-47 0.5263390126933203 2307 48-49 0.34942452942222246 2307 50-51 0.3828651071423792 2307 52-53 0.42679167457888667 2307 54-55 0.46054311706409123 2307 56-57 0.4544506328254414 2307 58-59 0.5038886854923064 2307 60-61 0.42799093127404575 2307 62-63 0.25251550615178076 2307 64-65 0.20462555366500368 2307 66-67 0.3971757802260072 2307 68-69 0.4899290989542351 2307 70-71 0.4237323553911523 2307 72-73 0.29359065702394815 2307 74-75 0.24101602754250706 2307 76-77 0.3281336583127725 2307 78-79 0.3600101147964807 2307 80-81 0.4254181608251244 2307 82-83 0.42863325924189866 2307 84-85 0.5066674218272667 2307 86-87 0.31905296056341825 2307 88-89 0.3721558274507615 2307 90-91 0.49247484184326495 2307 92-93 0.543487233495167 2307 94-95 0.22972633820225496 2307 96-97 0.4424989516315492 2307 98-99 0.3926020549601148 2307 100-101 0.48480870560082323 2201 1 0.10843945479740569 2201 2 0.1092481916202459 2201 3 0.0831809415062068 2201 4 0.13442560291055372 2201 5 0.143986071887781 2201 6 0.1910095095625337 2201 7 0.21295275560235893 2201 8 0.278189107906222 2201 9 0.24857642892342113 2201 10-11 0.28165985266316085 2201 12-13 0.24696969583620643 2201 14-15 0.3701194618847694 2201 16-17 0.39173231194359914 2201 18-19 0.38857262357698374 2201 20-21 0.4684455761768973 2201 22-23 0.438503108073256 2201 24-25 0.33023331864054484 2201 26-27 0.39339592938978996 2201 28-29 0.4025365088438093 2201 30-31 0.3700769690007206 2201 32-33 0.42394910807876585 2201 34-35 0.3016564040754375 2201 36-37 0.29805168333145815 2201 38-39 0.4603554098966427 2201 40-41 0.4148070824252912 2201 42-43 0.1903950362248068 2201 44-45 0.3439780446381917 2201 46-47 0.36012232256555876 2201 48-49 0.328809921955731 2201 50-51 0.2978949956044019 2201 52-53 0.3685971527454015 2201 54-55 0.32714966466567574 2201 56-57 0.3461089292564168 2201 58-59 0.2529477976852377 2201 60-61 0.1776848372536861 2201 62-63 0.22015935201236658 2201 64-65 0.15930408773736815 2201 66-67 0.25554192243937024 2201 68-69 0.4465040718273414 2201 70-71 0.24062061946536772 2201 72-73 0.1388531036255074 2201 74-75 0.2755768528844058 2201 76-77 -0.08773905789591652 2201 78-79 0.12087309220540021 2201 80-81 0.11060993086726256 2201 82-83 0.215160173520907 2201 84-85 0.2502076771622228 2201 86-87 0.1913833441523778 2201 88-89 0.15696745438019377 2201 90-91 0.30368661980351774 2201 92-93 0.19379528260705925 2201 94-95 0.21417022463098334 2201 96-97 0.3111638440526363 2201 98-99 0.2994941803401403 2201 100-101 0.30287185750166046 1101 1 0.040818311509731586 1101 2 0.04064773996162785 1101 3 0.04947308754282176 1101 4 0.08923281528733185 1101 5 0.12815358747119632 1101 6 0.13174838875307415 1101 7 0.10632332658800436 1101 8 0.0015963231750504292 1101 9 0.07660060447690853 1101 10-11 0.14412037990403803 1101 12-13 0.1496352196440185 1101 14-15 0.24525714596008896 1101 16-17 0.2037360197526894 1101 18-19 0.2507953045383644 1101 20-21 0.29027403066972823 1101 22-23 0.2429171760075306 1101 24-25 0.2715216875585682 1101 26-27 0.2767614991213776 1101 28-29 0.1927358958286831 1101 30-31 0.20299444531706712 1101 32-33 0.2921466172248728 1101 34-35 0.36145105846784276 1101 36-37 0.3893357889671165 1101 38-39 0.36355820700877217 1101 40-41 0.24416908097227719 1101 42-43 0.25927347383903054 1101 44-45 0.3690045093997938 1101 46-47 0.2964944537629606 1101 48-49 0.31489541597542114 1101 50-51 0.42037361312414845 1101 52-53 0.3286108052215724 1101 54-55 0.3286797093378908 1101 56-57 0.42502826944989636 1101 58-59 0.3406933789551161 1101 60-61 0.24806240451008676 1101 62-63 0.3081325576566485 1101 64-65 0.2969844233349761 1101 66-67 0.36595968920740063 1101 68-69 0.4730665648153618 1101 70-71 0.29533025004571556 1101 72-73 0.11601736764585269 1101 74-75 0.058830829330140944 1101 76-77 -0.0827796887220913 1101 78-79 0.22704552726763438 1101 80-81 0.12995426297047175 1101 82-83 0.19648001593403563 1101 84-85 0.2069884651663969 1101 86-87 0.3124720318941243 1101 88-89 0.21615727116875405 1101 90-91 0.32105488313175456 1101 92-93 0.4173705360230464 1101 94-95 0.24117924035127913 1101 96-97 0.3533740792212754 1101 98-99 0.3689787021319937 1101 100-101 0.20783755927034875 2304 1 0.06507890667806748 2304 2 0.04846786982866291 2304 3 0.06667877267957323 2304 4 0.07410170960719853 2304 5 0.07103632839932317 2304 6 0.13743930187174414 2304 7 0.042665522179383686 2304 8 0.06488997822228981 2304 9 0.06348169943801452 2304 10-11 0.021905988530711795 2304 12-13 0.05117398145282692 2304 14-15 0.11411752222195304 2304 16-17 0.14773480552448603 2304 18-19 0.09445977558983287 2304 20-21 0.13282171250025243 2304 22-23 0.17919989585490015 2304 24-25 0.21650230695191652 2304 26-27 0.3042486720122284 2304 28-29 0.2826274906778323 2304 30-31 0.2346845955979049 2304 32-33 0.1387922448123362 2304 34-35 0.2158409638783354 2304 36-37 0.24400431479045892 2304 38-39 0.11064121579361341 2304 40-41 0.3177524863613357 2304 42-43 0.2898355948021205 2304 44-45 0.3370813690328731 2304 46-47 0.21821990836905059 2304 48-49 0.29032329891882114 2304 50-51 0.21220426610380372 2304 52-53 0.18830283550164495 2304 54-55 0.30663095679510377 2304 56-57 0.26016039046062645 2304 58-59 0.2452976352448104 2304 60-61 0.2503224083451201 2304 62-63 0.2028604907413012 2304 64-65 0.21262265836286787 2304 66-67 0.2467411930400516 2304 68-69 0.2229571413564031 2304 70-71 0.3372468786002685 2304 72-73 0.057868699426116166 2304 74-75 0.13857574314912569 2304 76-77 0.0173819304711742 2304 78-79 0.2776019939409622 2304 80-81 0.31052545044873625 2304 82-83 0.2649115117869627 2304 84-85 0.24515626090450837 2304 86-87 0.2752177359440111 2304 88-89 0.18023407065614805 2304 90-91 0.2566084110036222 2304 92-93 0.2647574889355262 2304 94-95 0.021718434494395922 2304 96-97 0.32621696321275806 2304 98-99 0.3459605470680849 2304 100-101 0.20460212345830087 2202 1 0.07153436951013248 2202 2 0.09118514875171257 2202 3 0.030301433199063865 2202 4 0.08582021233966941 2202 5 0.04207081661488132 2202 6 -0.043789729841797964 2202 7 0.018858340739086543 2202 8 0.1124646464222323 2202 9 0.02646040937408145 2202 10-11 0.09712846336876879 2202 12-13 0.0591853834531193 2202 14-15 0.16163667061939435 2202 16-17 -0.008276301073536274 2202 18-19 0.021560626453883458 2202 20-21 0.07530227397557354 2202 22-23 0.011782437052964667 2202 24-25 -0.004396678657769826 2202 26-27 0.039432137924919175 2202 28-29 0.0766408470806752 2202 30-31 0.06257525191846014 2202 32-33 0.0959972909161877 2202 34-35 0.12583630865498918 2202 36-37 0.18619685904804584 2202 38-39 0.12118002162991814 2202 40-41 0.20531097337418203 2202 42-43 0.2556726514349563 2202 44-45 0.23675319556843277 2202 46-47 0.3457160575893994 2202 48-49 0.315968502324381 2202 50-51 0.228109238884592 2202 52-53 0.2936976195414829 2202 54-55 0.3118545657668932 2202 56-57 0.4354455723440296 2202 58-59 0.26871307898122865 2202 60-61 0.17512044022745954 2202 62-63 0.4252790177389585 2202 64-65 0.22598275373560028 2202 66-67 0.3405655051747587 2202 68-69 0.3969513229866024 2202 70-71 0.3590553471504734 2202 72-73 0.2431479073260192 2202 74-75 0.3098495203947067 2202 76-77 0.3696412303677832 2202 78-79 0.3960600204427749 2202 80-81 0.3240291588022899 2202 82-83 0.28154371485492646 2202 84-85 0.35558897248638033 2202 86-87 0.25762046419502127 2202 88-89 0.3150945722070091 2202 90-91 0.31806837080781136 2202 92-93 0.31649647201319553 2202 94-95 0.17309739604990426 2202 96-97 0.2885902303619119 2202 98-99 0.5214176952756233 2202 100-101 0.5934949319954264 1102 1 0.09641420585798244 1102 2 0.06670620130583416 1102 3 0.07895615953793111 1102 4 0.11163486744865025 1102 5 0.0845573874253418 1102 6 0.1834636513705803 1102 7 0.18543268326068585 1102 8 0.17729974333315113 1102 9 0.17668307637433145 1102 10-11 0.15389280132812644 1102 12-13 0.15767513311831038 1102 14-15 0.20264389662714422 1102 16-17 0.1827310721034081 1102 18-19 0.1669355399919752 1102 20-21 0.12629404721727155 1102 22-23 0.15033605815114015 1102 24-25 0.19761012055928973 1102 26-27 0.2629618440280268 1102 28-29 0.26146063839474465 1102 30-31 0.1966790650724377 1102 32-33 0.23814566690406025 1102 34-35 0.18794553763554944 1102 36-37 0.24186360911059523 1102 38-39 0.20664588573388443 1102 40-41 0.15011928549062503 1102 42-43 0.2217975392184428 1102 44-45 0.30203068309584324 1102 46-47 0.3076645778030809 1102 48-49 0.39687241555341757 1102 50-51 0.2431926102366404 1102 52-53 0.3614581458185455 1102 54-55 0.3022179265227578 1102 56-57 0.39559656794367015 1102 58-59 0.3171282138617997 1102 60-61 0.26083325424974646 1102 62-63 0.303051464721257 1102 64-65 0.17608474199150237 1102 66-67 0.30281572224932773 1102 68-69 0.2697538660703813 1102 70-71 0.24106420673633977 1102 72-73 0.09725996195522413 1102 74-75 -0.049153261103178636 1102 76-77 -0.07773753900336544 1102 78-79 0.06939973110812048 1102 80-81 0.2516013085028064 1102 82-83 0.23399422921128377 1102 84-85 0.16517321949516628 1102 86-87 0.25450724692912985 1102 88-89 0.14174249932882077 1102 90-91 0.2339385087053536 1102 92-93 0.1652068283098309 1102 94-95 -0.026508190492947392 1102 96-97 0.19333261067655982 1102 98-99 0.31296400206453967 1102 100-101 0.28843672657665564 2305 1 0.01861442841276073 2305 2 0.008495848671628892 2305 3 0.019458826358082604 2305 4 0.006760917868817273 2305 5 0.026482869947116683 2305 6 -0.0012706259019736876 2305 7 -0.03700467524436135 2305 8 0.09085563144181918 2305 9 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0.06717977012819532 1213 96-97 -0.07172391031371461 1213 98-99 0.15238401602481844 1213 100-101 0.2403562646184092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 14.0 10 14.0 11 17.0 12 45.0 13 134.0 14 391.0 15 913.0 16 1773.0 17 2948.0 18 4435.0 19 6339.0 20 8566.0 21 11526.0 22 14945.0 23 19073.0 24 24341.0 25 30081.0 26 38556.0 27 52733.0 28 74737.0 29 85748.0 30 74803.0 31 82036.0 32 107042.0 33 148299.0 34 217104.0 35 344701.0 36 589404.0 37 927844.0 38 402134.0 39 865.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.955329701761116 26.395831980782962 19.550406132596077 16.09843218485985 2 29.5897619609459 27.652553946675045 28.461616523473175 14.296067568905881 3 34.80830575223349 21.057054057325487 27.51136765167975 16.623272538761277 4 29.44570072107153 21.71757134890274 28.297226760852762 20.53950116917297 5 34.203335163243025 19.959413907232136 28.872031847764557 16.96521908176028 6 30.99092666382195 20.813837051636465 30.98906211218854 17.206174172353037 7 31.095280422452383 24.810396495376523 25.173831231790057 18.92049185038104 8 28.70969358997715 30.157410950847975 23.052002039391557 18.08089341978332 9 31.67216047126084 24.561402436265958 24.872843691885592 18.89359340058761 10-11 26.344143045956002 29.971704664802523 27.984306588530742 15.69984570071073 12-13 24.000982404745866 29.389444926035374 27.722780297130058 18.8867923720887 14-15 23.304480670407184 29.674706042736748 27.961916685719736 19.05889660113634 16-17 24.86245110901086 27.664016354257477 28.17753221395027 19.2960003227814 18-19 22.044441129685985 27.758833389779326 30.561437893313414 19.63528758722128 20-21 23.920898383769966 27.811101968355196 30.503682642308082 17.764317005566756 22-23 21.605262803967765 32.47708129811919 28.008362361243734 17.909293536669313 24-25 21.288518274439994 27.999559232220427 30.47433887889707 20.23758361444251 26-27 21.282099326193833 27.959868735565006 28.248431025650117 22.509600912591043 28-29 23.25685818770472 30.363612021650805 28.12170264741879 18.257827143225686 30-31 20.867144888499812 28.205882568704144 30.399909645661833 20.527062897134215 32-33 20.783240064996438 30.499143528905442 28.16593225747685 20.55168414862127 34-35 20.706686465555922 28.29267591891829 30.456977152212218 20.54366046331357 36-37 22.961021089607296 28.1536598397586 28.21416606858371 20.671153002050396 38-39 22.854833345763677 30.48511354202455 28.14016476523155 18.51988834698022 40-41 22.681842690529667 30.368777746667952 28.225506210485264 18.72387335231712 42-43 20.51653276536849 30.33923530152551 30.30351843949866 18.84071349360734 44-45 20.34653761931602 28.1504197992153 30.533332069921297 20.969710511547383 46-47 22.565394975642374 28.12874582099532 28.16586875537301 21.1399904479893 48-49 20.26677092189921 30.462070663589348 30.22659465189609 19.04456376261536 50-51 22.473892810479892 28.14416850918698 30.20891712074406 19.17302155958907 52-53 20.35647812899189 28.248249949487647 27.960276046568566 23.434995874951895 54-55 22.268539704695097 28.195271979049068 30.34985264101358 19.18633567524225 56-57 20.18869683778033 28.199332338770816 30.25517254029201 21.356798283156838 58-59 20.062064767834908 28.47894545533474 30.260579089779917 21.198410687050437 60-61 22.11097909406281 28.318186695065783 30.318316300688714 19.252517910182693 62-63 20.017386594348867 28.38541873631738 30.203094251358557 21.394100417975196 64-65 22.032644441903788 28.4270886383965 30.257507384036543 19.282759535663168 66-67 20.087688083188578 32.394682891545344 27.95743598491727 19.560193040348807 68-69 20.010804768773667 32.66528636306102 27.87748052603998 19.44642834212533 70-71 19.92440929956694 32.54071885303914 28.022061172672398 19.51281067472152 72-73 19.873000771772066 32.48428215644335 27.945975038240856 19.696742033543728 74-75 19.839316469786695 32.206809040260005 28.15713038133371 19.796744108619595 76-77 19.923780723144606 31.802456582877536 28.143903780041413 20.129858913936445 78-79 19.856759748324627 31.731327397162705 28.231281391789402 20.180631462723266 80-81 19.841162491385717 31.444908407004647 28.26659929606278 20.447329805546858 82-83 19.908416177893063 31.396268147234093 28.125803807596945 20.5695118672759 84-85 19.916921444165062 31.159517307446592 28.321216500656277 20.60234474773207 86-87 20.03605550236192 30.91809769775498 28.31787660788464 20.727970191998455 88-89 20.0749810308111 31.0436049835315 28.202705272303398 20.678708713354002 90-91 20.129474285668863 30.78544553847038 28.221303779856722 20.863776396004035 92-93 20.10565317394406 30.850641190552512 28.12530046372875 20.918405171774683 94-95 20.00826046701599 30.73376762438048 28.262613147685805 20.995358760917718 96-97 20.128455664345413 30.65980542560626 28.124489824618905 21.08724908542942 98-99 20.13712786676736 30.667462629981014 28.10755900167244 21.087850501579187 100-101 20.249412611350103 30.529520301689477 27.989430290712864 21.23163679624756 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 9.0 1 5.5 2 3.0 3 9.5 4 15.0 5 13.5 6 14.0 7 11.0 8 14.5 9 17.0 10 17.0 11 29.0 12 32.0 13 32.0 14 56.0 15 104.0 16 200.0 17 351.5 18 653.5 19 1166.5 20 1974.5 21 3223.5 22 4985.5 23 7433.0 24 10743.5 25 14998.0 26 20204.0 27 26316.0 28 33500.0 29 41586.5 30 50153.5 31 59214.0 32 69139.5 33 80344.5 34 92609.0 35 105628.5 36 119553.5 37 134213.0 38 147055.0 39 156866.5 40 163738.5 41 166514.5 42 166595.5 43 164137.0 44 158299.5 45 150779.5 46 141975.5 47 131985.5 48 120300.0 49 108004.0 50 97065.0 51 86880.5 52 76486.0 53 65899.5 54 55899.0 55 46793.0 56 38752.5 57 31799.5 58 25993.0 59 21094.0 60 16747.5 61 13088.0 62 10182.5 63 7731.0 64 5795.5 65 4341.0 66 3212.5 67 2347.0 68 1747.0 69 1321.5 70 931.0 71 627.5 72 471.5 73 382.5 74 281.5 75 194.5 76 151.5 77 122.0 78 91.0 79 71.5 80 55.5 81 40.0 82 34.5 83 28.5 84 22.0 85 18.0 86 13.0 87 10.0 88 7.5 89 4.5 90 3.5 91 4.0 92 2.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18731102310699102 2 0.0 3 0.0 4 0.026776184112552896 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.584963032971386E-5 48-49 0.0032247573331898745 50-51 1.0698247076933234E-4 52-53 0.002659278559123404 54-55 0.009200492486162582 56-57 0.013571490577595303 58-59 0.014213385402211298 60-61 0.003087208442200733 62-63 0.0023841807771451205 64-65 0.00972012162989934 66-67 0.016444734078257372 68-69 0.024025206292770065 70-71 0.02014327092485429 72-73 0.01590982172441071 74-75 0.020250253395623623 76-77 0.014534332814519295 78-79 0.0063272489855005125 80-81 0.0032858901736294937 82-83 7.794437156051357E-4 84-85 0.002506446458024358 86-87 0.015160944429025384 88-89 0.014366217503310344 90-91 0.01733116026463184 92-93 0.017682674097159647 94-95 0.017193611373642698 96-97 0.01925684473847982 98-99 0.027326379676509457 100-101 0.015848688883971094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3271564.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.81620516007634 #Duplication Level Percentage of deduplicated Percentage of total 1 97.73967259846313 87.78606486382255 2 1.5631525547567648 2.8079286110906208 3 0.28092268817605487 0.7569422938602208 4 0.11050279244910113 0.396997659094792 5 0.05096293721456994 0.22886488122119514 6 0.04699917267000408 0.2532772400929763 7 0.026890770434535957 0.16906588679825577 8 0.02692489954592706 0.19346338412251446 9 0.022465881192536973 0.18160201748617205 >10 0.11737519955474034 2.0496560871711944 >50 0.010361413402470843 0.6414728212200789 >100 0.0033828561309963267 0.5294911401469599 >500 2.464806157915753E-4 0.15545385215803223 >1k 1.058380123003442E-4 0.16341570174938477 >5k 0.0 0.0 >10k+ 3.391738107875088E-5 3.686303559965108 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 121008 3.6987813779586762 TruSeq Adapter, Index 6 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001650586691869699 0.0 0.0 2 0.00201738373450741 0.0 0.0 3 0.0024758800378045487 0.0 0.0 4 0.0027204113995630223 0.0 0.0 5 0.003331739803959207 0.0 0.0 6 0.00403476746901482 0.0 0.0 7 0.004890627235169478 0.0 0.0 8 0.005899319102423184 0.0 3.056642021980924E-5 9 0.006541213927039178 0.0 3.056642021980924E-5 10-11 0.009368607797371533 0.0 3.056642021980924E-5 12-13 0.012089019196934555 0.0 6.113284043961848E-5 14-15 0.014885846647047101 0.0 6.113284043961848E-5 16-17 0.018141170400456784 0.0 7.64160505495231E-5 18-19 0.021839707247053704 0.0 9.169926065942772E-5 20-21 0.026394103859805278 0.0 1.3754889098914158E-4 22-23 0.03082623479167762 0.0 1.528321010990462E-4 24-25 0.036404606481792806 0.0 1.528321010990462E-4 26-27 0.042196943113446655 0.0 1.8339852131885544E-4 28-29 0.04878400667081555 0.0 1.8339852131885544E-4 30-31 0.07974779035348231 0.0 1.8339852131885544E-4 32-33 0.08853563616667746 0.0 1.8339852131885544E-4 34-35 0.09775141186294994 0.0 1.8339852131885544E-4 36-37 0.10753266633328891 0.0 1.8339852131885544E-4 38-39 0.1187505425539589 0.0 2.139649415386647E-4 40-41 0.13047276470825575 0.0 2.139649415386647E-4 42-43 0.14240895180409124 0.0 2.2924815164856928E-4 44-45 0.15660705399619262 0.0 2.445313617584739E-4 46-47 0.17127893570170108 0.0 2.5981457186837853E-4 48-49 0.18735687273732074 0.0 2.903809920881878E-4 50-51 0.2044129352199743 0.0 3.056642021980924E-4 52-53 0.22370034637867392 0.0 3.056642021980924E-4 54-55 0.24431739681693526 0.0 3.056642021980924E-4 56-57 0.2652553946675046 0.0 3.056642021980924E-4 58-59 0.28935701701082417 0.0 3.056642021980924E-4 60-61 0.31561357197964035 0.0 3.3623062241790164E-4 62-63 0.3457367791062623 0.0 3.3623062241790164E-4 64-65 0.37654773068783004 0.0 3.667970426377109E-4 66-67 0.4100790936689608 0.0 3.667970426377109E-4 68-69 0.4456584068048187 0.0 3.9736346285752013E-4 70-71 0.4857004172927688 0.0 4.279298830773294E-4 72-73 0.5311832505798451 0.0 4.279298830773294E-4 74-75 0.5800742397214298 0.0 4.279298830773294E-4 76-77 0.6345130341329102 0.0 5.043459336268525E-4 78-79 0.6919473377259318 0.0 6.571780347258986E-4 80-81 0.7561826698178609 0.0 6.724612448358033E-4 82-83 0.8279984741243026 0.0 7.335940852754218E-4 84-85 0.9075322995362463 0.0 7.64160505495231E-4 86-87 0.998100602647541 0.0 7.64160505495231E-4 88-89 1.0965091925452168 0.0 8.100101358249449E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 19340 0.0 70.36803 3 CGGAAGA 19320 0.0 70.317924 4 ATCGGAA 19640 0.0 69.31734 2 GATCGGA 19675 0.0 69.280235 1 GAAGAGC 20335 0.0 66.68109 6 AGAGCAC 20470 0.0 66.264534 8 GAGCACA 20530 0.0 65.79326 9 AAGAGCA 22195 0.0 60.964653 7 GGAAGAG 23670 0.0 57.5268 5 GCCGTCT 14565 0.0 44.46741 50-51 ATGCCGT 14535 0.0 44.460133 48-49 TCGTATG 14940 0.0 44.286144 44-45 CGTCTTC 14705 0.0 44.223408 52-53 TCTCGTA 14640 0.0 44.20412 42-43 TGCCGTC 14500 0.0 44.12623 50-51 CGTATGC 14740 0.0 43.855896 46-47 AATCTCG 14845 0.0 43.78299 40-41 CTCGTAT 14625 0.0 43.72983 44-45 CCGTCTT 14680 0.0 43.71627 52-53 TATGCCG 14730 0.0 43.645832 48-49 >>END_MODULE