FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_52150223-1.3410000003573e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_52150223-1.3410000003573e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271564
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT1210083.6987813779586762TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG193400.070.368033
CGGAAGA193200.070.3179244
ATCGGAA196400.069.317342
GATCGGA196750.069.2802351
GAAGAGC203350.066.681096
AGAGCAC204700.066.2645348
GAGCACA205300.065.793269
AAGAGCA221950.060.9646537
GGAAGAG236700.057.52685
GCCGTCT145650.044.4674150-51
ATGCCGT145350.044.46013348-49
TCGTATG149400.044.28614444-45
CGTCTTC147050.044.22340852-53
TCTCGTA146400.044.2041242-43
TGCCGTC145000.044.1262350-51
CGTATGC147400.043.85589646-47
AATCTCG148450.043.7829940-41
CTCGTAT146250.043.7298344-45
CCGTCTT146800.043.7162752-53
TATGCCG147300.043.64583248-49
Produced by FastQC (version 0.11.2)