##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_50331580-1.341000000357a8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3141398 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69149849843923 33.0 33.0 33.0 33.0 33.0 2 32.7224719694862 33.0 33.0 33.0 33.0 33.0 3 32.75527328915343 33.0 33.0 33.0 33.0 33.0 4 36.680991074674395 37.0 37.0 37.0 37.0 37.0 5 36.67879969363958 37.0 37.0 37.0 37.0 37.0 6 36.54526137725942 37.0 37.0 37.0 37.0 37.0 7 36.47995351114376 37.0 37.0 37.0 37.0 37.0 8 36.413216663409095 37.0 37.0 37.0 37.0 37.0 9 36.44037368076251 37.0 37.0 37.0 37.0 37.0 10-11 36.3815503479661 37.0 37.0 37.0 37.0 37.0 12-13 36.35359766575264 37.0 37.0 37.0 37.0 37.0 14-15 38.16738805461772 40.0 37.0 40.0 37.0 40.0 16-17 38.14746571430936 40.0 37.0 40.0 37.0 40.0 18-19 38.14449331157657 40.0 37.0 40.0 37.0 40.0 20-21 38.097414590574004 40.0 37.0 40.0 37.0 40.0 22-23 37.985727373608825 40.0 37.0 40.0 33.0 40.0 24-25 37.9582340410225 40.0 37.0 40.0 33.0 40.0 26-27 37.856102283123626 40.0 37.0 40.0 33.0 40.0 28-29 37.711556287996615 40.0 37.0 40.0 33.0 40.0 30-31 37.66734699009804 40.0 37.0 40.0 33.0 40.0 32-33 37.54538218334639 40.0 37.0 40.0 33.0 40.0 34-35 37.45850669033341 40.0 37.0 40.0 33.0 40.0 36-37 37.38353147229355 40.0 37.0 40.0 33.0 40.0 38-39 37.27725299373082 40.0 37.0 40.0 33.0 40.0 40-41 37.18990303043422 40.0 37.0 40.0 33.0 40.0 42-43 37.03288806448594 40.0 37.0 40.0 33.0 40.0 44-45 36.92305925578357 37.0 37.0 40.0 33.0 40.0 46-47 36.87050128636996 37.0 37.0 40.0 33.0 40.0 48-49 36.63879696237153 37.0 37.0 40.0 33.0 40.0 50-51 36.515361791151584 37.0 37.0 40.0 33.0 40.0 52-53 36.39099184503205 37.0 37.0 40.0 33.0 40.0 54-55 36.18998961608813 37.0 37.0 40.0 33.0 40.0 56-57 36.029622321017584 37.0 37.0 40.0 30.0 40.0 58-59 35.76789649067071 37.0 37.0 40.0 27.0 40.0 60-61 35.539104882603226 37.0 35.0 40.0 27.0 40.0 62-63 35.29690093391541 37.0 33.0 40.0 27.0 40.0 64-65 35.064155831257295 37.0 33.0 40.0 27.0 40.0 66-67 34.68710013821872 37.0 33.0 37.0 27.0 40.0 68-69 34.419783325767696 37.0 33.0 37.0 27.0 40.0 70-71 34.06054438183254 37.0 33.0 37.0 27.0 40.0 72-73 33.59927124802397 37.0 33.0 37.0 27.0 40.0 74-75 33.13933621273077 37.0 33.0 37.0 22.0 38.5 76-77 30.629689233901594 33.0 30.0 35.0 22.0 37.0 78-79 32.81484867565332 35.0 33.0 37.0 24.5 37.0 80-81 33.120347692333155 37.0 33.0 37.0 27.0 37.0 82-83 32.98059621862623 37.0 33.0 37.0 27.0 37.0 84-85 32.75982476591632 37.0 33.0 37.0 22.0 37.0 86-87 32.61853496436937 37.0 33.0 37.0 22.0 37.0 88-89 32.44564712908075 35.0 33.0 37.0 22.0 37.0 90-91 32.26504616734333 33.0 33.0 37.0 22.0 37.0 92-93 32.11168753529479 33.0 33.0 37.0 22.0 37.0 94-95 31.961926505332976 33.0 33.0 37.0 22.0 37.0 96-97 31.78959702018019 33.0 33.0 37.0 22.0 37.0 98-99 31.60789161386109 33.0 33.0 37.0 22.0 37.0 100-101 29.892659255528905 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.16124361059910086 2306 2 0.10792583204305117 2306 3 0.11842548282687915 2306 4 0.16684901634729954 2306 5 0.16660008782440627 2306 6 0.1599821520907625 2306 7 0.25337956928512995 2306 8 0.31870007062917693 2306 9 0.26461461629487104 2306 10-11 0.3190022918671147 2306 12-13 0.2472515750168256 2306 14-15 0.4834362355461792 2306 16-17 0.48883569555361817 2306 18-19 0.42822880333363145 2306 20-21 0.500502147370625 2306 22-23 0.493216458796347 2306 24-25 0.49139750323383424 2306 26-27 0.4935849078431289 2306 28-29 0.44600322498887834 2306 30-31 0.3383412291695933 2306 32-33 0.5197456335819837 2306 34-35 0.6386696427633822 2306 36-37 0.5796247145856981 2306 38-39 0.6320558602225788 2306 40-41 0.5614502351705752 2306 42-43 0.7282653963441632 2306 44-45 0.536336621629971 2306 46-47 0.6525846735402894 2306 48-49 0.644848772085922 2306 50-51 0.7628013205406248 2306 52-53 0.573135153921271 2306 54-55 0.5869181574586619 2306 56-57 0.6315087586128101 2306 58-59 0.5344206526881337 2306 60-61 0.6580280275536126 2306 62-63 0.6096528764671305 2306 64-65 0.631466231812361 2306 66-67 0.6378813041464966 2306 68-69 0.7552722853476794 2306 70-71 0.7406228102023178 2306 72-73 0.42720492071488536 2306 74-75 0.5735247191229718 2306 76-77 0.5719061407669841 2306 78-79 0.8042807173478295 2306 80-81 0.6669945182855272 2306 82-83 0.6047278024424898 2306 84-85 0.6367547349917473 2306 86-87 0.6382732275640493 2306 88-89 0.6906777310557217 2306 90-91 0.6564868515812137 2306 92-93 0.8393845172520003 2306 94-95 0.7104028271339402 2306 96-97 0.9031433048490349 2306 98-99 0.9361747883506943 2306 100-101 0.52279202369537 2307 1 0.13439300516562014 2307 2 0.12748959202649246 2307 3 0.13051871699224904 2307 4 0.20084463374401196 2307 5 0.17856881036775007 2307 6 0.20878710634989517 2307 7 0.17192530098041914 2307 8 0.22259596382084368 2307 9 0.12258064177241579 2307 10-11 0.20498926342376222 2307 12-13 0.18146287535549277 2307 14-15 0.46005609144541637 2307 16-17 0.46208636300279693 2307 18-19 0.4270317081245949 2307 20-21 0.4002238141593466 2307 22-23 0.5024956617024401 2307 24-25 0.4786295738603954 2307 26-27 0.3685110969762846 2307 28-29 0.5847140597809997 2307 30-31 0.44834673272048775 2307 32-33 0.5317324497563547 2307 34-35 0.4885621357244503 2307 36-37 0.5351165260600084 2307 38-39 0.5487334888284181 2307 40-41 0.528674094204213 2307 42-43 0.5210303792855626 2307 44-45 0.5429251043735306 2307 46-47 0.43848668220773845 2307 48-49 0.584577343610043 2307 50-51 0.5732482400870751 2307 52-53 0.57413544873598 2307 54-55 0.41393412667445517 2307 56-57 0.49322196650039984 2307 58-59 0.5694212812488715 2307 60-61 0.5802445367999738 2307 62-63 0.5536048959307394 2307 64-65 0.38232044842587953 2307 66-67 0.6339554149731583 2307 68-69 0.6524772223878514 2307 70-71 0.5537901200361119 2307 72-73 0.2830260362738386 2307 74-75 0.32666670816355037 2307 76-77 0.3248501288879524 2307 78-79 0.3425585226709913 2307 80-81 0.47818147591606674 2307 82-83 0.5954172143754661 2307 84-85 0.6598551567019797 2307 86-87 0.5889493891279969 2307 88-89 0.5168778575910906 2307 90-91 0.6302997078692414 2307 92-93 0.6804708142677853 2307 94-95 0.4399593822506489 2307 96-97 0.6493774714212357 2307 98-99 0.5408932643147288 2307 100-101 0.6257116840290351 2201 1 0.1284055511625226 2201 2 0.12271615089264998 2201 3 0.11549440979671033 2201 4 0.1634077812699246 2201 5 0.11347904345463178 2201 6 0.12262272254223916 2201 7 0.17557631897331305 2201 8 0.16764931034788333 2201 9 0.21796326235208596 2201 10-11 0.2341695712730072 2201 12-13 0.20135670310575193 2201 14-15 0.3785304579946924 2201 16-17 0.41763481486700016 2201 18-19 0.37271810130313554 2201 20-21 0.41546362659795477 2201 22-23 0.41771230732741316 2201 24-25 0.48058259012564974 2201 26-27 0.4937286225810027 2201 28-29 0.5356524953206829 2201 30-31 0.4814878473495199 2201 32-33 0.5234049679889949 2201 34-35 0.5786837345578419 2201 36-37 0.5673498248526343 2201 38-39 0.5687126207373794 2201 40-41 0.5267138944308485 2201 42-43 0.43693843756535955 2201 44-45 0.48571474790990266 2201 46-47 0.58448250499778 2201 48-49 0.4858184742223841 2201 50-51 0.6237141803813486 2201 52-53 0.5598267985351129 2201 54-55 0.6435567590350857 2201 56-57 0.5763683789666558 2201 58-59 0.5165928647275209 2201 60-61 0.55968713248879 2201 62-63 0.5870980024567771 2201 64-65 0.43037991350595206 2201 66-67 0.5554369776016728 2201 68-69 0.5334231467347337 2201 70-71 0.47194212665930735 2201 72-73 0.5580646027329692 2201 74-75 0.48584516625944474 2201 76-77 0.29106698822229404 2201 78-79 0.43484505125865525 2201 80-81 0.44892753710878 2201 82-83 0.5179780990963181 2201 84-85 0.5546553743622766 2201 86-87 0.5064467985753893 2201 88-89 0.3910353667814874 2201 90-91 0.46685693977622833 2201 92-93 0.6190051271921746 2201 94-95 0.4926692708883209 2201 96-97 0.48032797525453574 2201 98-99 0.5388818820461374 2201 100-101 0.5672002146604136 1101 1 0.04940161366700124 1101 2 0.10375371830662061 1101 3 0.10465269674795508 1101 4 0.13703722639686333 1101 5 0.12408824418084663 1101 6 0.11601755920118251 1101 7 0.06451441844556172 1101 8 0.05930256363018316 1101 9 0.0677776674202093 1101 10-11 0.12148069919289384 1101 12-13 0.14948908975176067 1101 14-15 0.368029522259377 1101 16-17 0.29350412357851496 1101 18-19 0.28608490124818076 1101 20-21 0.2690774489868133 1101 22-23 0.34270080465237385 1101 24-25 0.3195005733632499 1101 26-27 0.3764231591714946 1101 28-29 0.2888886119684102 1101 30-31 0.4427064670223686 1101 32-33 0.4157898743893398 1101 34-35 0.35267507212915916 1101 36-37 0.3814060476538472 1101 38-39 0.47793400105223327 1101 40-41 0.5075307826014921 1101 42-43 0.43553214525633877 1101 44-45 0.4741572104074905 1101 46-47 0.3927477417776899 1101 48-49 0.38721865021253876 1101 50-51 0.5217155733038155 1101 52-53 0.4780984098101584 1101 54-55 0.5363287761693769 1101 56-57 0.4989765064172147 1101 58-59 0.5289280045776152 1101 60-61 0.4691654596663639 1101 62-63 0.4782520381053885 1101 64-65 0.5926191132086771 1101 66-67 0.5381907256910878 1101 68-69 0.6034248771304718 1101 70-71 0.5196836067654402 1101 72-73 0.292863552570239 1101 74-75 0.45748542466930786 1101 76-77 0.31391343696154905 1101 78-79 0.3929251387681134 1101 80-81 0.4857540005456471 1101 82-83 0.3878593318634458 1101 84-85 0.4955565970420821 1101 86-87 0.5537389290244832 1101 88-89 0.45614396525007805 1101 90-91 0.5449386654756196 1101 92-93 0.6463850283536914 1101 94-95 0.6367590627521409 1101 96-97 0.7261597346858828 1101 98-99 0.8320009311214527 1101 100-101 0.44582278891759586 2304 1 0.03270022858495736 2304 2 0.05586628820027073 2304 3 0.05535140485744705 2304 4 0.11272776882641722 2304 5 0.111314094325202 2304 6 0.11377028413996726 2304 7 0.09915574523721205 2304 8 0.13034740520456722 2304 9 0.08055924062124831 2304 10-11 0.14778518083780767 2304 12-13 0.13436887723851498 2304 14-15 0.1823670857575337 2304 16-17 0.19409439374260984 2304 18-19 0.20145462382063073 2304 20-21 0.18690743953061428 2304 22-23 0.12169168078985138 2304 24-25 0.22658784106963736 2304 26-27 0.29620334820413063 2304 28-29 0.28399976800027815 2304 30-31 0.3301534186541346 2304 32-33 0.2691045694154681 2304 34-35 0.30143757190074894 2304 36-37 0.2379695983427368 2304 38-39 0.2372029043157653 2304 40-41 0.18633833065909045 2304 42-43 0.33461490527899684 2304 44-45 0.28539795406681634 2304 46-47 0.3345619902610437 2304 48-49 0.4966680264231158 2304 50-51 0.38507319810738494 2304 52-53 0.36336075654663347 2304 54-55 0.3120876419278886 2304 56-57 0.29819185880914034 2304 58-59 0.30794814582674945 2304 60-61 0.3151777241750082 2304 62-63 0.1951226469110665 2304 64-65 0.16921890124361738 2304 66-67 0.36115435929303885 2304 68-69 0.23747336413847364 2304 70-71 0.2468493963352998 2304 72-73 0.07368870880310396 2304 74-75 0.06754893995454836 2304 76-77 0.033096560613927295 2304 78-79 0.4383316011900007 2304 80-81 0.3896519421090687 2304 82-83 0.34827177293350076 2304 84-85 0.2794083368458189 2304 86-87 0.3149022129297592 2304 88-89 0.3003367963541237 2304 90-91 0.29066396058214394 2304 92-93 0.36288289956664244 2304 94-95 0.21667237520873783 2304 96-97 0.1930446191557813 2304 98-99 0.4241938844251827 2304 100-101 0.30537180926441465 2202 1 0.08016837077140337 2202 2 0.07765954775761941 2202 3 0.06271642833162616 2202 4 0.05744922254875462 2202 5 0.04375142665249854 2202 6 0.09315806918355918 2202 7 0.11670059768523089 2202 8 0.15187658025487139 2202 9 0.11723450051393769 2202 10-11 0.14168556229321183 2202 12-13 0.10017831721168591 2202 14-15 0.12534579132426416 2202 16-17 0.049400186834489546 2202 18-19 0.14039713304263302 2202 20-21 0.18116831729720673 2202 22-23 0.22855630604136223 2202 24-25 0.2123665759790967 2202 26-27 0.1635941513563779 2202 28-29 0.15271099368773378 2202 30-31 0.1741434002101343 2202 32-33 0.21572596445235348 2202 34-35 0.25137836247754564 2202 36-37 0.21704509297720875 2202 38-39 0.22971631813200588 2202 40-41 0.16493595809034645 2202 42-43 0.317058525332655 2202 44-45 0.2540478744222767 2202 46-47 0.25691223757827686 2202 48-49 0.3503060000985059 2202 50-51 0.24527293821469698 2202 52-53 0.39955552935011696 2202 54-55 0.34276179098891646 2202 56-57 0.3165824104523338 2202 58-59 0.26820761933445425 2202 60-61 0.21817330180746808 2202 62-63 0.2960160280311257 2202 64-65 0.17677255650853851 2202 66-67 0.3744649520160408 2202 68-69 0.4245835251042749 2202 70-71 0.312501643149524 2202 72-73 0.272770700754819 2202 74-75 0.3901978542738007 2202 76-77 0.4039917232564463 2202 78-79 0.5877735654757217 2202 80-81 0.5188207046845221 2202 82-83 0.3749631713238415 2202 84-85 0.4181926049544771 2202 86-87 0.48767321393579266 2202 88-89 0.4421218818672017 2202 90-91 0.353738239589525 2202 92-93 0.5795369907805963 2202 94-95 0.3077820541155134 2202 96-97 0.5639811759968332 2202 98-99 0.6349579479731311 2202 100-101 0.5817703044019034 1102 1 0.043671204411616316 1102 2 0.0483702295051458 1102 3 0.07284220847940759 1102 4 0.11613965034472784 1102 5 0.07608122889549662 1102 6 0.10050640367266794 1102 7 0.06932739411747946 1102 8 0.10832460627175067 1102 9 0.13113009820876442 1102 10-11 0.03526174775915081 1102 12-13 0.0600401623060236 1102 14-15 0.0859844962191687 1102 16-17 0.1283944836852129 1102 18-19 0.11964191412300096 1102 20-21 0.12543198931232524 1102 22-23 0.1350748741563379 1102 24-25 0.13659629213682223 1102 26-27 0.1429109690708117 1102 28-29 0.19425789820481754 1102 30-31 0.12575570562155747 1102 32-33 0.23503148347480618 1102 34-35 0.1361971649357585 1102 36-37 0.05159577568124263 1102 38-39 0.1323177074526427 1102 40-41 0.12422778716325666 1102 42-43 0.09024654991478087 1102 44-45 0.10824154237225514 1102 46-47 0.10608486246170656 1102 48-49 0.08325274679258854 1102 50-51 -0.028491080532766944 1102 52-53 -0.062448284705688195 1102 54-55 -0.0291364490455166 1102 56-57 -0.0019982586215334663 1102 58-59 0.0017181049988721497 1102 60-61 -0.09689132192815464 1102 62-63 0.08386485272803412 1102 64-65 -0.11687536668426901 1102 66-67 0.00208487358279541 1102 68-69 0.05173027658087648 1102 70-71 -0.0565835812700044 1102 72-73 -0.09677424293106895 1102 74-75 -0.1916805981043055 1102 76-77 -0.06900231055593764 1102 78-79 0.025782903336498464 1102 80-81 0.05654877087453514 1102 82-83 -0.0903507410126565 1102 84-85 0.007436130624626003 1102 86-87 0.06100979539397855 1102 88-89 0.0836897098879561 1102 90-91 -0.015859909577123688 1102 92-93 0.09028659024495056 1102 94-95 -0.28205510124448807 1102 96-97 -0.050555876539096545 1102 98-99 0.05858865411149594 1102 100-101 0.020550822826546522 2305 1 0.05028312157342896 2305 2 0.0687376843252352 2305 3 0.0315911301440579 2305 4 -0.0020697015082404846 2305 5 0.042685720279280304 2305 6 0.017279958207517154 2305 7 0.03136822708264475 2305 8 -0.016356961162351524 2305 9 0.024188097501443906 2305 10-11 0.0392576757004548 2305 12-13 0.047982582779134475 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-0.12426697772818329 1213 94-95 0.11901022592137878 1213 96-97 -0.10141406960960708 1213 98-99 0.08472326435108712 1213 100-101 0.22143951391069194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 13.0 10 13.0 11 14.0 12 47.0 13 132.0 14 401.0 15 899.0 16 1707.0 17 2791.0 18 3976.0 19 5511.0 20 7129.0 21 9178.0 22 11681.0 23 14645.0 24 18257.0 25 22971.0 26 29016.0 27 38999.0 28 55549.0 29 77108.0 30 75374.0 31 78943.0 32 103767.0 33 144871.0 34 214795.0 35 345367.0 36 606410.0 37 923871.0 38 346677.0 39 1285.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.44154936225694 25.262707020914505 22.005165476400062 16.290578140428497 2 29.08797293434324 24.97467687952943 31.555218409128678 14.38213177699865 3 36.02386580751627 20.206672315956144 27.000558350135833 16.768903526391753 4 28.665154855562815 20.977238773178662 31.750389415539175 18.60721695571935 5 32.43075216830214 19.146762046706595 31.797021580837576 16.62546420415369 6 27.925401365888693 20.3881201936208 35.07642138945781 16.610057051032694 7 31.987414520541492 22.69196707962506 27.113947357195745 18.206671042637705 8 28.48356050395397 30.182039970739144 24.075204733688633 17.259194791618253 9 31.605259823810925 25.177039012567015 24.58739707607887 18.63030408754319 10-11 26.707583693629395 28.990659572585198 27.749285509190496 16.552471224594907 12-13 25.54120808633608 27.995927290970453 27.237220498644234 19.22564412404923 14-15 24.56914087294892 28.452029956089614 27.90003049597663 19.07879867498483 16-17 24.79674654405459 27.65052374770723 28.15900755014169 19.39372215809649 18-19 22.550023906553708 28.085855405777938 29.755271379175767 19.608849308492587 20-21 23.58497713438412 27.999954160536166 29.80171566926572 18.61335303581399 22-23 22.10098815877517 30.999987903474825 28.168493772517838 18.730530165232167 24-25 21.945547810242445 28.03990452658339 29.795508241871932 20.219039421302234 26-27 22.00370662997812 27.966163472441252 28.25635274486073 21.7737771527199 28-29 22.890159094772457 29.85428780434698 28.088911370033344 19.16664173084722 30-31 21.26669400056917 28.48556598049658 29.63010417654815 20.617635842386097 32-33 21.20603629339549 30.043964502428537 28.199562742447785 20.550436461728186 34-35 22.43566399418348 28.645749440217383 28.60637206746805 20.312214498131087 36-37 21.163380125663796 28.559641280729153 29.793693763095284 20.483284830511767 38-39 21.221141033387045 30.073346325425813 28.199944737979717 20.505567903207425 40-41 21.121551614917944 28.331828695377027 30.94881641867729 19.59780327102774 42-43 21.081824079597684 29.775803639016768 29.537374761173208 19.60499752021234 44-45 20.69534010017196 28.340932922221253 30.058257501914753 20.905469475692033 46-47 22.279553421472492 28.174544056209406 28.380678526362164 21.165223995955937 48-49 20.854575259889124 30.035989974187366 29.39353129119026 19.715903474733242 50-51 22.179014276289454 28.288460788550317 29.517101863103925 20.015423072056308 52-53 20.75654291967808 28.525819835670756 28.04251912932655 22.675118115324615 54-55 21.960134360531548 28.437337736035868 29.733555208826456 19.86897269460613 56-57 20.8773021233324 28.286376867701872 29.438938871854102 21.397382137111627 58-59 20.694516432618432 28.6161853045641 29.654995880224693 21.03430238259277 60-61 21.88176573374815 28.420992146854363 29.742121785037927 19.95512033435956 62-63 20.59322804112855 28.746564145058056 29.258371039067477 21.401836774745913 64-65 21.82963500993526 28.55898064538517 29.569841381267718 20.04154296341185 66-67 20.838819478637504 30.8129778836509 27.97543289407557 20.37276974363603 68-69 20.823135653434534 31.127185055074236 27.932215392193495 20.117463899297736 70-71 20.624127990463375 31.067100875145748 28.121101627836044 20.187669506554837 72-73 20.52222631345773 31.234768306328792 27.87864981587052 20.364355564342958 74-75 20.560048524059845 30.828108930783166 28.250952810033404 20.360889735123585 76-77 20.889922211600684 30.231789212779486 28.186653952658357 20.691634622961466 78-79 20.7022936826868 30.32949583167924 28.44982782019443 20.518382665439532 80-81 20.46848550397647 30.12745549801555 28.56397486066643 20.840084137341556 82-83 20.42406264389596 30.275559225568543 28.255170386348272 21.04520774418722 84-85 20.32123787570627 29.964486233003544 28.73247112596405 20.981804765326135 86-87 20.52137434887107 29.730544612977656 28.654018875535638 21.09406216261564 88-89 20.529770857238187 30.20327776157589 28.476094932573982 20.79085644861194 90-91 20.738271348262646 29.647288362431063 28.472516841522616 21.14192344778368 92-93 20.536343389672393 29.982377129430194 28.196656589632273 21.284622891265137 94-95 20.192175592053747 29.99646112144721 28.57307736453362 21.23828592196542 96-97 20.508289502235055 29.998556077140186 28.199781612637366 21.293372807987392 98-99 20.527201158531305 29.94869015655966 28.1016122129782 21.422496471930835 100-101 20.580646558064338 29.643733376463523 28.089088465072503 21.686531600399633 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 100.0 1 63.5 2 40.5 3 85.0 4 89.0 5 52.0 6 45.0 7 56.5 8 72.0 9 76.0 10 74.5 11 69.5 12 68.5 13 85.5 14 106.0 15 124.0 16 169.0 17 266.0 18 421.5 19 657.5 20 1070.5 21 1691.5 22 2577.5 23 3889.5 24 5638.0 25 8055.0 26 11143.5 27 14841.0 28 19375.5 29 24555.0 30 30368.5 31 37380.0 32 46356.5 33 57417.5 34 70526.5 35 87348.0 36 109014.0 37 134856.5 38 159793.5 39 176714.5 40 184867.5 41 181863.0 42 172798.0 43 168310.0 44 162998.5 45 157224.0 46 154404.5 47 145003.0 48 127183.0 49 110291.5 50 98010.0 51 87326.0 52 76247.5 53 64739.0 54 53445.5 55 42688.5 56 33601.5 57 26543.0 58 21078.5 59 16675.0 60 12914.5 61 9879.5 62 7458.0 63 5564.0 64 4054.5 65 2943.5 66 2182.0 67 1559.0 68 1140.5 69 843.0 70 600.5 71 407.0 72 286.5 73 233.0 74 185.5 75 138.5 76 105.5 77 84.0 78 59.5 79 39.5 80 29.5 81 26.5 82 21.5 83 13.5 84 7.5 85 7.0 86 9.0 87 8.0 88 5.5 89 2.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18991544528900828 2 0.0 3 0.0 4 0.025275371029076864 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 6.366592198759915E-5 48-49 0.003390210345839655 50-51 1.5916480496899787E-5 52-53 0.002626219281988465 54-55 0.009454389415158474 56-57 0.013974669876278014 58-59 0.01432483244720981 60-61 0.0034697927483241537 62-63 0.0024352215160256675 64-65 0.009947800310562367 66-67 0.016919218768204475 68-69 0.02462279532870397 70-71 0.021137086099882917 72-73 0.01711021653416727 74-75 0.021901077163734108 76-77 0.016091561782365683 78-79 0.006525757003728913 80-81 0.0033583773848458553 82-83 7.162416223604905E-4 84-85 0.0025784698404977655 86-87 0.016139311223856385 88-89 0.015088823511061 90-91 0.018288036090937855 92-93 0.018876945869323147 94-95 0.018224370168950257 96-97 0.020643675204479024 98-99 0.02769467606460563 100-101 0.01655313971677578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3141398.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.25348450589344 #Duplication Level Percentage of deduplicated Percentage of total 1 94.5744375666544 74.00779284775774 2 3.4659670897624535 5.424480039133254 3 0.6610664928133836 1.5519226965821227 4 0.3398856409562827 1.0638894295339252 5 0.24167926158923142 0.9456122176084343 6 0.14806018209291905 0.69517350992088 7 0.06353901424155503 0.3480504486529888 8 0.06334949950509004 0.3965855262382133 9 0.06322035226828877 0.44524915710152946 >10 0.3088610322863976 4.34976044266344 >50 0.03454524020860768 1.9545149889733575 >100 0.03320884677658852 5.187843222308594 >500 0.001972063295065997 1.0400876012547156 >1k 1.6708240993897647E-4 0.1840285086250308 >5k 0.0 0.0 >10k+ 4.063513990603083E-5 2.4050093636457754 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 75633 2.4076223388440434 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0011141536347829852 0.0 3.1832960993799575E-5 2 0.0011778195567705844 0.0 6.366592198759915E-5 3 0.0014006502837271813 0.0 9.549888298139873E-5 4 0.0015598150886961793 0.0 1.273318439751983E-4 5 0.001878144698634175 0.0 1.5916480496899787E-4 6 0.002323806152547369 0.0 1.5916480496899787E-4 7 0.0029604653724233606 0.0 1.5916480496899787E-4 8 0.0036289575532931517 0.0 1.5916480496899787E-4 9 0.004074619007206346 0.0 1.5916480496899787E-4 10-11 0.005411603368945928 0.0 1.9099776596279746E-4 12-13 0.006764504211182409 0.0 2.2283072695659703E-4 14-15 0.008260653377890989 0.0 2.387472074534968E-4 16-17 0.009709053103108871 0.0 2.864966489441962E-4 18-19 0.011348450594289548 0.0 2.864966489441962E-4 20-21 0.01349717546137102 0.0 2.864966489441962E-4 22-23 0.015502652003980395 0.0 2.864966489441962E-4 24-25 0.01720571541714867 0.0 2.864966489441962E-4 26-27 0.018956528271807646 0.0 2.864966489441962E-4 28-29 0.021248501463361217 0.0 2.864966489441962E-4 30-31 0.0376902258166587 0.0 3.1832960993799573E-4 32-33 0.04063477470858516 0.0 3.1832960993799573E-4 34-35 0.04314957862709533 0.0 3.1832960993799573E-4 36-37 0.04609412751902178 0.0 3.1832960993799573E-4 38-39 0.04859301495703505 0.0 3.1832960993799573E-4 40-41 0.05166489569293671 0.0 3.5016257093179533E-4 42-43 0.055484851012192665 0.0 3.660790514286951E-4 44-45 0.059877799629337 0.0 3.819955319255949E-4 46-47 0.06449357897343794 0.0 3.819955319255949E-4 48-49 0.07020759547182497 0.0 3.819955319255949E-4 50-51 0.07732226225393918 0.0 3.819955319255949E-4 52-53 0.08510542121692316 0.0 3.819955319255949E-4 54-55 0.09215642207704977 3.1832960993799575E-5 3.819955319255949E-4 56-57 0.10041707545494076 3.1832960993799575E-5 4.1382849291939447E-4 58-59 0.11006246263606204 3.1832960993799575E-5 4.1382849291939447E-4 60-61 0.12149049563283608 3.1832960993799575E-5 4.1382849291939447E-4 62-63 0.13302994399308843 3.1832960993799575E-5 4.1382849291939447E-4 64-65 0.14724336107681993 3.1832960993799575E-5 4.1382849291939447E-4 66-67 0.16280967900278792 3.1832960993799575E-5 4.2974497341629426E-4 68-69 0.18291219387037239 3.1832960993799575E-5 4.7749441490699366E-4 70-71 0.20279187801100018 3.1832960993799575E-5 5.093273759007932E-4 72-73 0.2259662736144863 3.1832960993799575E-5 5.093273759007932E-4 74-75 0.25157589073399805 3.1832960993799575E-5 5.093273759007932E-4 76-77 0.28063938412133704 3.1832960993799575E-5 5.411603368945928E-4 78-79 0.31302942193252814 3.1832960993799575E-5 5.729932978883924E-4 80-81 0.34830034271365806 3.1832960993799575E-5 6.04826258882192E-4 82-83 0.38899878334423077 3.1832960993799575E-5 6.844086613666909E-4 84-85 0.4357454865636255 3.1832960993799575E-5 7.003251418635907E-4 86-87 0.4876332129835188 3.1832960993799575E-5 7.003251418635907E-4 88-89 0.5436114748911154 3.1832960993799575E-5 7.003251418635907E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 10735 0.0 71.59405 3 CGGAAGA 10810 0.0 71.09732 4 GATCGGA 10965 0.0 70.25194 1 ATCGGAA 10995 0.0 69.94425 2 GAAGAGC 11805 0.0 65.130516 6 GGAAGAG 15055 0.0 51.417446 5 TTGTGTT 22780 0.0 50.169456 1 AGAGCAC 15820 0.0 48.660908 8 GAGCACA 16045 0.0 48.067326 9 GCCGTCT 8595 0.0 44.958893 50-51 ATGCCGT 8525 0.0 44.9373 48-49 TATGCCG 8465 0.0 44.8069 48-49 TGCCGTC 8565 0.0 44.783607 50-51 TCGTATG 8590 0.0 44.730877 44-45 CGTATGC 8495 0.0 44.72898 46-47 AAGAGCA 17270 0.0 44.602806 7 TCTCGTA 8585 0.0 44.314342 42-43 CGTCTTC 8740 0.0 44.216175 52-53 CTCGTAT 8555 0.0 44.10887 44-45 TATCTCG 8640 0.0 43.889927 40-41 >>END_MODULE