FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_50331580-1.341000000357a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_50331580-1.341000000357a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3141398
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT756332.4076223388440434TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG107350.071.594053
CGGAAGA108100.071.097324
GATCGGA109650.070.251941
ATCGGAA109950.069.944252
GAAGAGC118050.065.1305166
GGAAGAG150550.051.4174465
TTGTGTT227800.050.1694561
AGAGCAC158200.048.6609088
GAGCACA160450.048.0673269
GCCGTCT85950.044.95889350-51
ATGCCGT85250.044.937348-49
TATGCCG84650.044.806948-49
TGCCGTC85650.044.78360750-51
TCGTATG85900.044.73087744-45
CGTATGC84950.044.7289846-47
AAGAGCA172700.044.6028067
TCTCGTA85850.044.31434242-43
CGTCTTC87400.044.21617552-53
CTCGTAT85550.044.1088744-45
TATCTCG86400.043.88992740-41
Produced by FastQC (version 0.11.2)