FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_50330665-2.34100000035758.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_50330665-2.34100000035758.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2579697
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT206440.8002490214936095TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC76000.29460824275098974No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG69040.2676283299937938No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA36140.14009397227658907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG200.002074494771.2489244
CGGCGCC301.2901833E-463.4455261
CGGAAGA40700.058.2362254
TCGGAAG41100.057.9005853
GATCGGA42150.056.333311
ATCGGAA42550.055.8158452
ACGACGG352.7643252E-454.3818781
CGGCGGC451.5006955E-552.8712731
GGACGGG2100.052.115971
GAAGAGC46950.050.375796
CGGCGAT502.798554E-547.5841451
CGACGGG554.9654802E-543.1811642
CCGGGCG1006.366463E-1042.749352
ATGCCGT26300.042.3520248-49
GGCGCGT459.6280203E-442.221593
TCGTATG26900.042.11041344-45
CGTATGC26300.041.89766346-47
TGCCGTC26600.041.87558450-51
GCCGTCT27000.041.87095650-51
TATGCCG26450.041.75267848-49
Produced by FastQC (version 0.11.2)