##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_50330665-2.34100000035758.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2579697 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70014966874017 33.0 33.0 33.0 33.0 33.0 2 32.7302504906584 33.0 33.0 33.0 33.0 33.0 3 32.76055366192231 33.0 33.0 33.0 33.0 33.0 4 36.696207345281245 37.0 37.0 37.0 37.0 37.0 5 36.69270615890161 37.0 37.0 37.0 37.0 37.0 6 36.56852529580024 37.0 37.0 37.0 37.0 37.0 7 36.5211321329598 37.0 37.0 37.0 37.0 37.0 8 36.43833209869221 37.0 37.0 37.0 37.0 37.0 9 36.45454911952838 37.0 37.0 37.0 37.0 37.0 10-11 36.41598199323409 37.0 37.0 37.0 37.0 37.0 12-13 36.392712206123434 37.0 37.0 37.0 37.0 37.0 14-15 38.232941310549265 40.0 37.0 40.0 37.0 40.0 16-17 38.228886764608404 40.0 37.0 40.0 37.0 40.0 18-19 38.26627216297108 40.0 37.0 40.0 37.0 40.0 20-21 38.216215702851926 40.0 37.0 40.0 37.0 40.0 22-23 38.11972064936309 40.0 37.0 40.0 37.0 40.0 24-25 38.102295540910426 40.0 37.0 40.0 37.0 40.0 26-27 38.028602777768086 40.0 37.0 40.0 37.0 40.0 28-29 37.911801269684 40.0 37.0 40.0 33.0 40.0 30-31 37.881178874883375 40.0 37.0 40.0 33.0 40.0 32-33 37.77736939648339 40.0 37.0 40.0 33.0 40.0 34-35 37.696167999575145 40.0 37.0 40.0 33.0 40.0 36-37 37.61651717236559 40.0 37.0 40.0 33.0 40.0 38-39 37.53790096278749 40.0 37.0 40.0 33.0 40.0 40-41 37.46032266580145 40.0 37.0 40.0 33.0 40.0 42-43 37.34109354703285 40.0 37.0 40.0 33.0 40.0 44-45 37.21105773274924 40.0 37.0 40.0 33.0 40.0 46-47 37.17363434542894 40.0 37.0 40.0 33.0 40.0 48-49 36.98426094227345 37.0 37.0 40.0 33.0 40.0 50-51 36.83362135165486 37.0 37.0 40.0 33.0 40.0 52-53 36.71628896727019 37.0 37.0 40.0 33.0 40.0 54-55 36.55255210204919 37.0 37.0 40.0 33.0 40.0 56-57 36.39882377659082 37.0 37.0 40.0 33.0 40.0 58-59 36.17022832526456 37.0 37.0 40.0 33.0 40.0 60-61 35.931296001041986 37.0 37.0 40.0 33.0 40.0 62-63 35.68733847424717 37.0 35.0 40.0 27.0 40.0 64-65 35.46402833356011 37.0 33.0 40.0 27.0 40.0 66-67 35.14913398744116 37.0 33.0 37.0 27.0 40.0 68-69 34.90022781745298 37.0 33.0 37.0 27.0 40.0 70-71 34.60896609175418 37.0 33.0 37.0 27.0 40.0 72-73 34.30272179252059 37.0 33.0 37.0 27.0 40.0 74-75 33.95135475212787 37.0 33.0 37.0 27.0 38.5 76-77 31.357877688736313 33.0 30.0 35.0 24.5 37.0 78-79 33.65410957178304 37.0 33.0 37.0 27.0 37.0 80-81 33.99675019973276 37.0 33.0 37.0 27.0 37.0 82-83 33.898423148144914 37.0 33.0 37.0 27.0 37.0 84-85 33.706494599947206 37.0 33.0 37.0 27.0 37.0 86-87 33.575274344235 37.0 33.0 37.0 27.0 37.0 88-89 33.44130163348642 37.0 33.0 37.0 27.0 37.0 90-91 33.29513485498491 37.0 33.0 37.0 27.0 37.0 92-93 33.15073630740355 37.0 33.0 37.0 27.0 37.0 94-95 33.03060126828848 35.0 33.0 37.0 27.0 37.0 96-97 32.868159710229534 33.0 33.0 37.0 27.0 37.0 98-99 32.69749528723722 33.0 33.0 37.0 27.0 37.0 100-101 31.08893195596227 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11223964874115211 2306 2 0.0631857571144181 2306 3 0.07203135175537767 2306 4 0.1282562579436899 2306 5 0.17580009718628986 2306 6 0.19082385448767525 2306 7 0.23747719797786715 2306 8 0.17696208257343216 2306 9 0.21785489027470106 2306 10-11 0.18068881080723287 2306 12-13 0.13840072179622354 2306 14-15 0.3947095255898958 2306 16-17 0.33667744728813176 2306 18-19 0.3039124837833853 2306 20-21 0.4143776299936661 2306 22-23 0.389920244457052 2306 24-25 0.36734164310458794 2306 26-27 0.505576000751617 2306 28-29 0.42155977568378233 2306 30-31 0.3967694809889366 2306 32-33 0.4642807842781025 2306 34-35 0.5106196775222358 2306 36-37 0.46032124142241315 2306 38-39 0.5512872040828682 2306 40-41 0.566327585041769 2306 42-43 0.4448003902140414 2306 44-45 0.5158220811024066 2306 46-47 0.5128628392127368 2306 48-49 0.44805845361683794 2306 50-51 0.5454548772375034 2306 52-53 0.5049396809179143 2306 54-55 0.547719681779121 2306 56-57 0.5201867595503344 2306 58-59 0.6196074234900237 2306 60-61 0.6078529888904214 2306 62-63 0.43069353783669584 2306 64-65 0.3563886475328175 2306 66-67 0.5184933756442689 2306 68-69 0.6741709210823856 2306 70-71 0.5484595285284755 2306 72-73 0.31749495488473656 2306 74-75 0.4493667568878337 2306 76-77 0.47311510467931583 2306 78-79 0.4612034512465826 2306 80-81 0.45378693540895654 2306 82-83 0.3982671697385456 2306 84-85 0.5471448002995629 2306 86-87 0.44627772581775815 2306 88-89 0.5423312463022185 2306 90-91 0.40267879699442233 2306 92-93 0.584866456162267 2306 94-95 0.3108691084929589 2306 96-97 0.4560926671633183 2306 98-99 0.6477677699698035 2306 100-101 0.5383377201219197 2307 1 0.13429333496847562 2307 2 0.12436200998218538 2307 3 0.09436356940001644 2307 4 0.13559779591852106 2307 5 0.14974349872699833 2307 6 0.22007496759737677 2307 7 0.15948496247096244 2307 8 0.24303936300643159 2307 9 0.19485213403283552 2307 10-11 0.25302523299459523 2307 12-13 0.22797092374869266 2307 14-15 0.42154607453874604 2307 16-17 0.4815284767446286 2307 18-19 0.3301117866108072 2307 20-21 0.41981258759343376 2307 22-23 0.5322596457739337 2307 24-25 0.4285780725131261 2307 26-27 0.473472675911772 2307 28-29 0.4365121611912741 2307 30-31 0.44514329203335024 2307 32-33 0.5794986968179785 2307 34-35 0.5497872593412296 2307 36-37 0.4927166960147531 2307 38-39 0.4760602760030608 2307 40-41 0.4749867860661112 2307 42-43 0.5188406681702631 2307 44-45 0.5575086372880733 2307 46-47 0.5335199607859167 2307 48-49 0.569756519933577 2307 50-51 0.5198173263257999 2307 52-53 0.5160231056101736 2307 54-55 0.48117255541995263 2307 56-57 0.523295620319324 2307 58-59 0.5143217662324702 2307 60-61 0.6376294353859677 2307 62-63 0.5680681400853942 2307 64-65 0.4914920151995972 2307 66-67 0.6292575989558173 2307 68-69 0.7232865447567463 2307 70-71 0.512616817814461 2307 72-73 0.4451372130086497 2307 74-75 0.41167404737733904 2307 76-77 0.40245987436884434 2307 78-79 0.5152483932754706 2307 80-81 0.5050017043888744 2307 82-83 0.5045275306539949 2307 84-85 0.5152767110282142 2307 86-87 0.5007560952429557 2307 88-89 0.47113817838963 2307 90-91 0.529932486597879 2307 92-93 0.5448528078102086 2307 94-95 0.41195647513939804 2307 96-97 0.44670450159123476 2307 98-99 0.5301656159935106 2307 100-101 0.4825056129832923 2201 1 0.10764246703014635 2201 2 0.06268574075874511 2201 3 0.06392773319281275 2201 4 0.0861845134308794 2201 5 0.11051401443646824 2201 6 0.16854110252300103 2201 7 0.13331271321325033 2201 8 0.19298102326995092 2201 9 0.1949607934089741 2201 10-11 0.22994330813686048 2201 12-13 0.24130843098186716 2201 14-15 0.405330818589853 2201 16-17 0.3574472305768097 2201 18-19 0.285488571621336 2201 20-21 0.34549302381770985 2201 22-23 0.3889633687373717 2201 24-25 0.25864127605024834 2201 26-27 0.4345842613296824 2201 28-29 0.34071339835588077 2201 30-31 0.4514645081214894 2201 32-33 0.4170046364326936 2201 34-35 0.4331897121110515 2201 36-37 0.3291903715561304 2201 38-39 0.5704645768446497 2201 40-41 0.4127378991779409 2201 42-43 0.446060876744518 2201 44-45 0.3992712648442591 2201 46-47 0.41695604621977367 2201 48-49 0.3888830710246083 2201 50-51 0.4377023146880461 2201 52-53 0.5523153372409197 2201 54-55 0.6046738537270215 2201 56-57 0.5026529259807333 2201 58-59 0.5513767694361889 2201 60-61 0.5037011488494656 2201 62-63 0.4957833524842883 2201 64-65 0.421702081369709 2201 66-67 0.44712421870922725 2201 68-69 0.38920035717444534 2201 70-71 0.4398008065966721 2201 72-73 0.42408489006953687 2201 74-75 0.3776468463135174 2201 76-77 0.3082845343555469 2201 78-79 0.4073828342445438 2201 80-81 0.5235962557248186 2201 82-83 0.36774995380982745 2201 84-85 0.6328534862076509 2201 86-87 0.6408150127713483 2201 88-89 0.5134390148081138 2201 90-91 0.5091979492151708 2201 92-93 0.6610191670398322 2201 94-95 0.48452239731686575 2201 96-97 0.499249933714907 2201 98-99 0.4468488833981965 2201 100-101 0.562118871841836 1101 1 0.059809516808293495 1101 2 0.082355057371295 1101 3 0.09417048755578605 1101 4 0.14779191308091555 1101 5 0.09664515931408602 1101 6 0.10001135045870058 1101 7 0.10428186450605637 1101 8 0.1359380291604282 1101 9 0.16655565686101426 1101 10-11 0.21074821547657763 1101 12-13 0.20365592736481375 1101 14-15 0.3450648579899678 1101 16-17 0.282335425693617 1101 18-19 0.42281811994497787 1101 20-21 0.3670866797550545 1101 22-23 0.3264997313957494 1101 24-25 0.35421825950290753 1101 26-27 0.3164596853320063 1101 28-29 0.3596044168615933 1101 30-31 0.44870698365834016 1101 32-33 0.4200805056786123 1101 34-35 0.5068535971029604 1101 36-37 0.4347205693031313 1101 38-39 0.4520919114645352 1101 40-41 0.47757924272464436 1101 42-43 0.5386190734640408 1101 44-45 0.6142022938004672 1101 46-47 0.4727245634575965 1101 48-49 0.5705646340316264 1101 50-51 0.6353560038649775 1101 52-53 0.5487454847113966 1101 54-55 0.639002398715725 1101 56-57 0.5234529154424266 1101 58-59 0.4745173614696654 1101 60-61 0.6266404152513658 1101 62-63 0.7772594184453325 1101 64-65 0.5357880695887545 1101 66-67 0.7236330083184583 1101 68-69 0.7253987935526922 1101 70-71 0.5719907332895531 1101 72-73 0.5005787504061914 1101 74-75 0.6561019300601529 1101 76-77 0.40946658146618375 1101 78-79 0.7081363234131359 1101 80-81 0.665302495941205 1101 82-83 0.4134960990935994 1101 84-85 0.6402006642458105 1101 86-87 0.5401060918723388 1101 88-89 0.3856104116391563 1101 90-91 0.4839532079416671 1101 92-93 0.6884099912454502 1101 94-95 0.5934138768736474 1101 96-97 0.5402351778853642 1101 98-99 0.7416493251290248 1101 100-101 0.5188986277482321 2304 1 0.0802763763408123 2304 2 0.06249554307202487 2304 3 0.08035675763951389 2304 4 0.13328850891088706 2304 5 0.12348172068733021 2304 6 0.18388977613887647 2304 7 0.18711768086915015 2304 8 0.15033548532866092 2304 9 0.14476748403215822 2304 10-11 0.13476055403240395 2304 12-13 0.1504086211096336 2304 14-15 0.21981744916092083 2304 16-17 0.2435331509832679 2304 18-19 0.27919176952972435 2304 20-21 0.24025916107750334 2304 22-23 0.32295148557748377 2304 24-25 0.33365118800690396 2304 26-27 0.28017485661348474 2304 28-29 0.32217541720643084 2304 30-31 0.2346073291028148 2304 32-33 0.28068498964098154 2304 34-35 0.21796005190062573 2304 36-37 0.2137498156754205 2304 38-39 0.30670898366911103 2304 40-41 0.3091165212376552 2304 42-43 0.3352336636510884 2304 44-45 0.40913217579328176 2304 46-47 0.3695247988376238 2304 48-49 0.4395120170147635 2304 50-51 0.33757092444783865 2304 52-53 0.3032808352406988 2304 54-55 0.37298754844994875 2304 56-57 0.34527098315331983 2304 58-59 0.267749713535558 2304 60-61 0.33280712881857966 2304 62-63 0.22802801383800642 2304 64-65 0.16315140172764586 2304 66-67 0.11127076602684838 2304 68-69 0.3077345623404071 2304 70-71 0.2845858587367118 2304 72-73 0.21686451038739563 2304 74-75 0.17207915866287493 2304 76-77 0.07219326463914655 2304 78-79 0.3902248352081159 2304 80-81 0.33628300083404383 2304 82-83 0.3415530194888632 2304 84-85 0.3440260619388411 2304 86-87 0.34805846790812467 2304 88-89 0.288793170592605 2304 90-91 0.26615886988385995 2304 92-93 0.402429678678601 2304 94-95 0.3278197818888984 2304 96-97 0.2718189520501113 2304 98-99 0.4286269660516808 2304 100-101 0.36338012025190025 2202 1 0.04130465391288851 2202 2 0.03655985843233367 2202 3 9.76620808209816E-4 2202 4 0.08412171686241976 2202 5 0.10354295593662499 2202 6 0.12163801681811748 2202 7 0.14329704657178866 2202 8 0.08951552503494042 2202 9 0.1430041877506767 2202 10-11 0.11529028909148309 2202 12-13 0.04345274449511294 2202 14-15 0.015823090730428646 2202 16-17 0.028611921336406 2202 18-19 0.09264627673030645 2202 20-21 0.12908709198418222 2202 22-23 0.16393647278854218 2202 24-25 0.12570730278569187 2202 26-27 0.10114646161064655 2202 28-29 0.14797789132579453 2202 30-31 0.08581093324860944 2202 32-33 0.12330850294918605 2202 34-35 0.12619616846532722 2202 36-37 0.10076185110526126 2202 38-39 0.18774550585924743 2202 40-41 0.26471698998210513 2202 42-43 0.19457546423164018 2202 44-45 0.16663092503335264 2202 46-47 0.21413240272408984 2202 48-49 0.2057777065596298 2202 50-51 0.08482017001635711 2202 52-53 0.25597703329746224 2202 54-55 0.31739295124356914 2202 56-57 0.2638397835124593 2202 58-59 0.27446589684008416 2202 60-61 0.32782518991437115 2202 62-63 0.3046115248465284 2202 64-65 0.12372249443311745 2202 66-67 0.2338718451666182 2202 68-69 0.34181639445601064 2202 70-71 0.21802065958759442 2202 72-73 0.20026151321185637 2202 74-75 0.23859512251866732 2202 76-77 0.1969432655565697 2202 78-79 0.3367204443985372 2202 80-81 0.13221310751255544 2202 82-83 0.17651876273384204 2202 84-85 0.20993939803668837 2202 86-87 0.24112827927272207 2202 88-89 0.29081915704158234 2202 90-91 0.23524430443272593 2202 92-93 0.306559082485073 2202 94-95 0.06826630012797352 2202 96-97 0.1277442514068099 2202 98-99 0.18828197649201428 2202 100-101 0.281257180030277 1102 1 0.07135100996453048 1102 2 0.09501352234405402 1102 3 0.06770817773794136 1102 4 0.10078646182203954 1102 5 0.13107448779087605 1102 6 0.10730357486504971 1102 7 0.18058096180920558 1102 8 0.08856306732089081 1102 9 0.05787611064059206 1102 10-11 0.1307913751358214 1102 12-13 0.11750777770706122 1102 14-15 0.14074897440782053 1102 16-17 0.08581899441239216 1102 18-19 0.07538934748382786 1102 20-21 0.13018675618919673 1102 22-23 0.11920295758336152 1102 24-25 0.05330829595374098 1102 26-27 0.017320387665598957 1102 28-29 0.06729530098410663 1102 30-31 0.07950251008946907 1102 32-33 0.2038622642895973 1102 34-35 0.05218647261371956 1102 36-37 -0.009451219562436108 1102 38-39 0.08772102741612997 1102 40-41 0.10985044414044154 1102 42-43 0.10429273448223597 1102 44-45 0.17262963618361482 1102 46-47 0.26455846196714816 1102 48-49 0.2043972492381343 1102 50-51 0.1188297203406421 1102 52-53 0.1241843119774444 1102 54-55 0.14406253218830045 1102 56-57 0.06838921037732604 1102 58-59 0.11288466404448627 1102 60-61 -0.10140514962327529 1102 62-63 0.10928073189796095 1102 64-65 0.07040977000851711 1102 66-67 0.13338699661392184 1102 68-69 0.08797605883267323 1102 70-71 -0.1309860604167028 1102 72-73 0.03878233612039139 1102 74-75 -0.06221490498599991 1102 76-77 -0.15166148184343342 1102 78-79 0.19723977546537697 1102 80-81 0.18463438375738406 1102 82-83 -0.0057234328033999304 1102 84-85 0.10016393551165947 1102 86-87 0.17493041583781377 1102 88-89 -0.028771825338786527 1102 90-91 0.17521157570947565 1102 92-93 0.060765288740576295 1102 94-95 -0.16566543038974402 1102 96-97 0.005125908769052501 1102 98-99 0.18638834876062305 1102 100-101 0.17876722608034612 2305 1 -0.0027709632423409403 2305 2 0.018190810656491863 2305 3 -0.014979635036269201 2305 4 -0.024240628510362683 2305 5 -0.02860157889757886 2305 6 -0.03836246610612193 2305 7 -0.012055656413707538 2305 8 0.006973163067122812 2305 9 0.006609553770665855 2305 10-11 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-0.16512468740286224 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 9.0 10 19.0 11 8.0 12 20.0 13 45.0 14 170.0 15 365.0 16 765.0 17 1312.0 18 2068.0 19 2843.0 20 3894.0 21 5288.0 22 6854.0 23 9049.0 24 11139.0 25 13671.0 26 17755.0 27 23552.0 28 31337.0 29 38521.0 30 43181.0 31 54025.0 32 73964.0 33 105316.0 34 160153.0 35 279131.0 36 544901.0 37 871143.0 38 278837.0 39 356.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.06143408466775 26.013373252950377 23.569960242205244 13.355232420176625 2 31.415627494236727 22.889199778113476 33.69000312827436 12.00516959937543 3 38.43052110383506 18.931874557360807 27.08314193488615 15.554462403917979 4 30.649991178871165 18.98617118418323 34.62822334710464 15.735614289840969 5 30.51536672717765 17.49845039940737 36.58522686966725 15.400956003747726 6 25.493575408274694 18.33882041185457 40.71249452939628 15.455109650474455 7 34.15156896333174 19.065843779327572 29.731786329944953 17.050800927395738 8 30.223743331096635 28.73426607853558 24.78732967476413 16.254660915603655 9 29.741167276622022 26.67510951867603 25.803689348012576 17.78003385668937 10-11 24.1923179350133 28.969700705160335 30.922565712174727 15.915415647651642 12-13 24.20565283442203 27.75198017441583 30.04738540999195 17.99498158117019 14-15 22.849214462008522 28.481639510376606 31.00650967923752 17.662636348377347 16-17 21.73825065501879 29.172922246294817 32.023392669759275 17.06543442892712 18-19 20.426740039624807 29.40541079049206 32.35536964224868 17.812479527634448 20-21 21.04908057031504 29.408744515344242 31.873394433532308 17.668780480808405 22-23 20.31804510374668 30.182769526808766 31.67216925088489 17.827016118559662 24-25 20.347060139233406 29.288924241878018 31.80400643951596 18.560009179372617 26-27 20.26869822308589 29.339472814055295 31.082293773260965 19.30953518959785 28-29 20.317424875867204 29.986777516894424 30.786716424448297 18.90908118279007 30-31 19.411620822135312 30.206764592895986 30.94200597977204 19.439608605196657 32-33 19.274879956832137 30.863818502715628 31.114894501175915 18.74640703927632 34-35 19.77955162951308 30.11285821551911 31.705235149709445 18.402355005258368 36-37 20.136725359606185 30.358797951852484 31.410762581807088 18.09371410673424 38-39 20.62953517409215 30.588631145440726 30.655034292787096 18.126799387680027 40-41 20.376230231690002 30.273846114485536 30.597391864238322 18.75253178958614 42-43 19.78335052527487 30.671974266745284 31.304315971992057 18.240359235987793 44-45 19.51242723467136 30.248319860820867 31.551651221054257 18.687601683453522 46-47 20.29492616670217 29.906366892384433 30.878574281897116 18.920132659016282 48-49 19.97089088656808 30.80220243064392 30.611068479683613 18.61583820310439 50-51 20.443048328175102 30.15165352816766 30.25654960894617 19.148748534711064 52-53 19.902105793427445 29.974436172128353 29.9793981403861 20.1440598940581 54-55 20.640988463378456 30.031037768692247 30.257792588928282 19.070181179001008 56-57 20.11982643447056 30.3352751933266 30.14681423045042 19.39808414175242 58-59 20.133388774678426 30.33767850029223 30.645339195383247 18.883593529646095 60-61 20.373455460250504 30.724549026083963 30.323190139290357 18.57880537437517 62-63 19.870722120980588 30.660748004467298 30.189596443220502 19.27893343133162 64-65 20.3249652828123 30.282847189322847 30.35540242861363 19.036785099251226 66-67 20.37785644455998 30.485937004152593 29.889422574574965 19.24678397671246 68-69 20.384970844325156 30.730878105684738 30.01265412705426 18.871496922935844 70-71 19.666814457039045 31.415761329469717 30.079269792778767 18.83815442071247 72-73 19.559923861159632 31.470197273571955 29.9445901702268 19.025288695041613 74-75 20.094637719342632 30.698052051908835 29.787656423358783 19.419653805389753 76-77 20.094689545999625 30.29445141669875 30.071210043458013 19.53964899384361 78-79 19.8474205750009 30.44922502693342 30.37322219073475 19.33013220733093 80-81 19.301619594068377 30.732455495955374 30.241119058519605 19.724805851456644 82-83 18.770638456971707 30.644085575713735 30.14729924887985 20.437976718434705 84-85 18.804822638014702 30.50000862528301 30.592444523439543 20.10272421326274 86-87 18.940441333833732 30.432521269567538 31.168155200382124 19.458882196216607 88-89 19.121869896240725 30.817018265703222 30.741803695788082 19.31930814226797 90-91 19.418543833604023 30.573430412643937 30.51482690155114 19.493198852200898 92-93 18.79276559645406 31.536215442451386 30.442125702162244 19.22889325893231 94-95 18.671610036009053 31.695255819363467 30.320533883888977 19.3126002607385 96-97 19.097548019882595 31.745872655226158 30.04811681413184 19.1084625107594 98-99 19.32513392684902 31.014668312571143 29.8320444572577 19.82815330332214 100-101 19.231440269226592 31.00450132597199 29.51301546191901 20.2510429428824 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 106.0 1 85.5 2 87.5 3 208.0 4 254.0 5 178.0 6 144.0 7 137.0 8 155.5 9 158.0 10 158.0 11 167.0 12 166.5 13 182.0 14 193.5 15 213.0 16 242.5 17 290.5 18 425.0 19 656.0 20 1082.0 21 1723.0 22 2669.5 23 4117.5 24 6054.5 25 8798.0 26 12898.5 27 18426.5 28 24275.0 29 30217.5 30 37144.5 31 44180.0 32 52886.5 33 65914.5 34 82506.5 35 102662.5 36 131276.5 37 163111.5 38 188351.0 39 197956.5 40 191291.5 41 173654.0 42 154220.5 43 142930.5 44 128498.0 45 112295.0 46 105018.5 47 93805.5 48 72915.0 49 54370.5 50 41291.5 51 32720.0 52 26912.5 53 20706.5 54 14827.0 55 10413.5 56 7260.5 57 4994.0 58 3492.0 59 2451.0 60 1658.5 61 1138.5 62 762.5 63 535.0 64 375.5 65 238.0 66 142.5 67 94.5 68 68.5 69 52.0 70 39.0 71 30.0 72 19.5 73 12.5 74 12.0 75 13.0 76 9.5 77 7.0 78 6.5 79 2.5 80 1.0 81 1.0 82 1.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18823916142089556 2 0.0 3 0.0 4 0.025661928513309895 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.9382121233617748E-5 48-49 0.003585692428219283 50-51 3.8764242467235495E-5 52-53 0.00292670030627628 54-55 0.010039938799013994 56-57 0.014846704864951193 58-59 0.015292493653324402 60-61 0.003314342730948635 62-63 0.0023258545480341294 64-65 0.010970280618227643 66-67 0.017967226383563652 68-69 0.02699929487842952 70-71 0.023006577904304264 72-73 0.01788969789862918 74-75 0.023200399116640444 76-77 0.01651356729104232 78-79 0.007287677583840273 80-81 0.0038764242467235494 82-83 6.396100007093857E-4 84-85 0.0027716433364073376 86-87 0.016474803048575085 88-89 0.015873957290332936 90-91 0.01938212123361775 92-93 0.020196170325429693 94-95 0.018781275475375597 96-97 0.021591683054250167 98-99 0.029867848821004947 100-101 0.01771525880752662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2579697.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.5769252280989 #Duplication Level Percentage of deduplicated Percentage of total 1 92.2056223862168 54.9332747051396 2 3.710680797750548 4.421419048658535 3 1.2582662979186452 2.248909114444078 4 0.5739390238678062 1.367740892418414 5 0.4251498982734814 1.2664561850086529 6 0.2161638874449121 0.772702785559243 7 0.22438113298234247 0.9357556587599594 8 0.17967201383049763 0.8563444906849207 9 0.10377108387137736 0.5564125894179446 >10 0.8810454668473936 10.564764218753727 >50 0.1067389955570081 4.447035944211365 >100 0.10769653260606317 13.102831937663082 >500 0.005681010217243634 2.2553987231631667 >1k 9.94894595460929E-4 0.8988327228489447 >5k 1.3105201363578607E-4 0.5662126647790805 >10k+ 6.552600681596124E-5 0.805908318489278 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 20644 0.8002490214936095 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 7600 0.29460824275098974 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 6904 0.2676283299937938 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 3614 0.14009397227658907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.5505696986894198E-4 0.0 0.0 2 1.9382121233617748E-4 0.0 0.0 3 2.7134969727064844E-4 0.0 0.0 4 3.8764242467235495E-4 0.0 0.0 5 4.6517090960682594E-4 0.0 0.0 6 6.202278794757679E-4 0.0 0.0 7 6.589921219430034E-4 0.0 0.0 8 7.365206068774743E-4 0.0 0.0 9 8.140490918119454E-4 0.0 0.0 10-11 0.0016474803048575084 0.0 0.0 12-13 0.002558440002837542 0.0 0.0 14-15 0.003663220913153754 0.0 1.9382121233617748E-5 16-17 0.004806766065937201 0.0 3.8764242467235495E-5 18-19 0.006376717885860238 0.0 3.8764242467235495E-5 20-21 0.00850875122155819 0.0 3.8764242467235495E-5 22-23 0.010214377890116554 0.0 3.8764242467235495E-5 24-25 0.012598378801851535 0.0 1.1629272740170649E-4 26-27 0.015389404259492492 0.0 1.1629272740170649E-4 28-29 0.018529307899338567 0.0 1.1629272740170649E-4 30-31 0.026456595483888228 0.0 1.5505696986894198E-4 32-33 0.030158580639509214 0.0 1.9382121233617748E-4 34-35 0.03370550882526126 0.0 2.3258545480341297E-4 36-37 0.037291201253480545 0.0 2.3258545480341297E-4 38-39 0.0412839182276058 0.0 3.1011393973788396E-4 40-41 0.04574180611133788 0.0 3.1011393973788396E-4 42-43 0.05018031187383634 0.0 3.4887818220511943E-4 44-45 0.055239045515810575 0.0 3.4887818220511943E-4 46-47 0.060200868551616715 0.0 3.4887818220511943E-4 48-49 0.06556971613332883 0.0 3.4887818220511943E-4 50-51 0.07146188098834863 0.0 3.4887818220511943E-4 52-53 0.07758663129817184 0.0 3.4887818220511943E-4 54-55 0.0837695279716959 1.9382121233617748E-5 3.4887818220511943E-4 56-57 0.09117349828293789 3.8764242467235495E-5 3.4887818220511943E-4 58-59 0.09820920829074112 3.8764242467235495E-5 3.4887818220511943E-4 60-61 0.10648537405749589 3.8764242467235495E-5 3.4887818220511943E-4 62-63 0.11534300346125921 3.8764242467235495E-5 3.4887818220511943E-4 64-65 0.12476271438079743 3.8764242467235495E-5 3.4887818220511943E-4 66-67 0.1355585559079225 3.8764242467235495E-5 3.4887818220511943E-4 68-69 0.14736226773919572 3.8764242467235495E-5 4.070245459059727E-4 70-71 0.1609685168451954 3.8764242467235495E-5 4.264066671395904E-4 72-73 0.17505931898203547 3.8764242467235495E-5 4.264066671395904E-4 74-75 0.19178608960664761 3.8764242467235495E-5 4.264066671395904E-4 76-77 0.21252495932661858 3.8764242467235495E-5 4.264066671395904E-4 78-79 0.2356865942007918 3.8764242467235495E-5 4.264066671395904E-4 80-81 0.2606895305921587 3.8764242467235495E-5 4.264066671395904E-4 82-83 0.29003406213985594 3.8764242467235495E-5 4.264066671395904E-4 84-85 0.32362327823771553 3.8764242467235495E-5 4.264066671395904E-4 86-87 0.3629496022207259 3.8764242467235495E-5 4.264066671395904E-4 88-89 0.40793550560395275 3.8764242467235495E-5 4.264066671395904E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCG 20 0.0020744947 71.248924 4 CGGCGCC 30 1.2901833E-4 63.445526 1 CGGAAGA 4070 0.0 58.236225 4 TCGGAAG 4110 0.0 57.900585 3 GATCGGA 4215 0.0 56.33331 1 ATCGGAA 4255 0.0 55.815845 2 ACGACGG 35 2.7643252E-4 54.381878 1 CGGCGGC 45 1.5006955E-5 52.871273 1 GGACGGG 210 0.0 52.11597 1 GAAGAGC 4695 0.0 50.37579 6 CGGCGAT 50 2.798554E-5 47.584145 1 CGACGGG 55 4.9654802E-5 43.181164 2 CCGGGCG 100 6.366463E-10 42.74935 2 ATGCCGT 2630 0.0 42.35202 48-49 GGCGCGT 45 9.6280203E-4 42.22159 3 TCGTATG 2690 0.0 42.110413 44-45 CGTATGC 2630 0.0 41.897663 46-47 TGCCGTC 2660 0.0 41.875584 50-51 GCCGTCT 2700 0.0 41.870956 50-51 TATGCCG 2645 0.0 41.752678 48-49 >>END_MODULE