##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_50330665-1.341000000356c2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2912488 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.675902183974664 33.0 33.0 33.0 33.0 33.0 2 32.68375904038059 33.0 33.0 33.0 33.0 33.0 3 32.73165863687679 33.0 33.0 33.0 33.0 33.0 4 36.63593532402537 37.0 37.0 37.0 37.0 37.0 5 36.64549725183417 37.0 37.0 37.0 37.0 37.0 6 36.53293438462236 37.0 37.0 37.0 37.0 37.0 7 36.33283982629285 37.0 37.0 37.0 37.0 37.0 8 36.27898552715067 37.0 37.0 37.0 37.0 37.0 9 36.37738970941683 37.0 37.0 37.0 37.0 37.0 10-11 36.27570654368361 37.0 37.0 37.0 35.0 37.0 12-13 36.27172798651874 37.0 37.0 37.0 35.0 37.0 14-15 38.04426833689959 40.0 37.0 40.0 35.0 40.0 16-17 38.05127042583523 40.0 37.0 40.0 35.0 40.0 18-19 38.00572465877971 40.0 37.0 40.0 35.0 40.0 20-21 38.034395849871316 40.0 37.0 40.0 33.0 40.0 22-23 37.69662123929781 40.0 37.0 40.0 33.0 40.0 24-25 37.89338599850025 40.0 37.0 40.0 33.0 40.0 26-27 37.81542876743183 40.0 37.0 40.0 33.0 40.0 28-29 37.53586023358723 40.0 37.0 40.0 33.0 40.0 30-31 37.462036581781625 40.0 37.0 40.0 33.0 40.0 32-33 37.42047091696172 40.0 37.0 40.0 33.0 40.0 34-35 37.23339117620399 40.0 37.0 40.0 33.0 40.0 36-37 37.20954300927592 40.0 37.0 40.0 33.0 40.0 38-39 37.19248216645013 40.0 37.0 40.0 33.0 40.0 40-41 37.12596360912045 40.0 37.0 40.0 33.0 40.0 42-43 36.41373526689209 37.0 37.0 40.0 33.0 40.0 44-45 36.20559003161558 37.0 37.0 40.0 33.0 40.0 46-47 36.647800780638406 37.0 37.0 40.0 33.0 40.0 48-49 36.299711277780375 37.0 37.0 40.0 33.0 40.0 50-51 36.295175636775156 37.0 37.0 40.0 33.0 40.0 52-53 36.285447013000564 37.0 37.0 40.0 33.0 40.0 54-55 35.895721287092 37.0 37.0 40.0 30.0 40.0 56-57 35.78374468839013 37.0 37.0 40.0 30.0 40.0 58-59 35.639394565745846 37.0 33.0 40.0 27.0 40.0 60-61 35.34457206347288 37.0 33.0 40.0 27.0 40.0 62-63 34.92404071707763 37.0 33.0 37.0 27.0 40.0 64-65 34.64165620596548 37.0 33.0 37.0 27.0 40.0 66-67 33.743899889029585 37.0 33.0 37.0 27.0 40.0 68-69 33.54598559719388 37.0 33.0 37.0 27.0 37.0 70-71 32.993732849714746 37.0 33.0 37.0 24.5 37.0 72-73 31.4692506887582 33.0 27.0 37.0 18.5 37.0 74-75 30.38457600512002 33.0 27.0 37.0 15.0 37.0 76-77 27.884025616586232 30.0 24.5 33.0 15.0 35.0 78-79 29.756149896583267 33.0 27.0 37.0 15.0 37.0 80-81 30.045830231746876 33.0 27.0 37.0 10.5 37.0 82-83 29.829970114898327 33.0 27.0 37.0 6.0 37.0 84-85 29.607957354674078 33.0 27.0 37.0 6.0 37.0 86-87 29.465139255509378 33.0 27.0 37.0 6.0 37.0 88-89 29.35009105616916 33.0 27.0 37.0 6.0 37.0 90-91 29.195957888925207 33.0 27.0 37.0 6.0 37.0 92-93 29.016395947382446 33.0 27.0 37.0 6.0 37.0 94-95 28.833603091240203 33.0 27.0 37.0 6.0 37.0 96-97 28.638005718821844 33.0 27.0 37.0 6.0 37.0 98-99 28.45423672131868 33.0 24.5 37.0 6.0 37.0 100-101 26.885971375676053 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.09223961135538872 2306 2 0.10366334768617236 2306 3 0.14020726143687057 2306 4 0.13342801733359266 2306 5 0.15377953694360258 2306 6 0.16499793475488644 2306 7 0.2549974749013302 2306 8 0.22138410014454735 2306 9 0.22819429745961628 2306 10-11 0.3086330843927101 2306 12-13 0.3604129804972658 2306 14-15 0.567675507734883 2306 16-17 0.5859639734382824 2306 18-19 0.5058161461195922 2306 20-21 0.6520020577463441 2306 22-23 0.6600804689375721 2306 24-25 0.5300440581960189 2306 26-27 0.612504534216491 2306 28-29 0.5918355424939321 2306 30-31 0.5851792897581305 2306 32-33 0.5278822036807327 2306 34-35 0.7216957471126051 2306 36-37 0.6938042209716713 2306 38-39 0.6196995301701165 2306 40-41 0.6272105223898166 2306 42-43 0.7389432118798211 2306 44-45 0.8070959379453342 2306 46-47 0.6276394069697702 2306 48-49 0.6319419069918482 2306 50-51 0.6634461449412541 2306 52-53 0.6616536937383302 2306 54-55 0.7424183177075747 2306 56-57 0.7746737385643669 2306 58-59 0.6709799564352323 2306 60-61 0.5722284041834342 2306 62-63 0.5622084013203121 2306 64-65 0.7562971745011353 2306 66-67 0.813658702658195 2306 68-69 0.8569289765259143 2306 70-71 0.7985946099086974 2306 72-73 0.17312853063177158 2306 74-75 0.2601564517943906 2306 76-77 0.51863726269168 2306 78-79 0.6080195549209577 2306 80-81 0.6674760608980819 2306 82-83 0.583755430819 2306 84-85 0.4629871231019109 2306 86-87 0.5021431877744185 2306 88-89 0.49715206820983937 2306 90-91 0.5902989253600452 2306 92-93 0.6047455758173221 2306 94-95 0.4483558810448649 2306 96-97 0.5316422982291265 2306 98-99 0.6539289191651143 2306 100-101 0.4634218956127505 2307 1 0.061190915642157506 2307 2 0.07444311105378887 2307 3 0.1230672129869177 2307 4 0.1833946641053501 2307 5 0.1452031931595883 2307 6 0.1454381973676746 2307 7 0.18556939499401892 2307 8 0.17948542374107745 2307 9 0.24165917124398106 2307 10-11 0.21068343021302383 2307 12-13 0.2214388555806437 2307 14-15 0.41112809348778256 2307 16-17 0.4540087720901127 2307 18-19 0.39635646841710326 2307 20-21 0.5106447569002341 2307 22-23 0.4436889371466819 2307 24-25 0.5192565709229058 2307 26-27 0.4908137010879585 2307 28-29 0.5218007849192361 2307 30-31 0.5359541379131727 2307 32-33 0.6209349017813608 2307 34-35 0.5195098818596406 2307 36-37 0.5027028622664815 2307 38-39 0.591115462480623 2307 40-41 0.557260025602254 2307 42-43 0.5906730156224071 2307 44-45 0.6296553593545937 2307 46-47 0.5553408429140134 2307 48-49 0.568875516513323 2307 50-51 0.5651517237075367 2307 52-53 0.545361168372331 2307 54-55 0.453257765799421 2307 56-57 0.5990946211961088 2307 58-59 0.5744669486762177 2307 60-61 0.5551375078593068 2307 62-63 0.5612106131659758 2307 64-65 0.5344687154202816 2307 66-67 0.5411221018787842 2307 68-69 0.5795305283287391 2307 70-71 0.5061254177589944 2307 72-73 -0.049473021171056786 2307 74-75 -0.006441167838371342 2307 76-77 0.16922252407566418 2307 78-79 0.0883811741324152 2307 80-81 0.09227681924516773 2307 82-83 0.19245044187822913 2307 84-85 0.3261714786824328 2307 86-87 0.28013356800122224 2307 88-89 0.16910937607769227 2307 90-91 0.21452249731524375 2307 92-93 0.3801091260051521 2307 94-95 0.026577276263061833 2307 96-97 0.1847539788807424 2307 98-99 0.3962046625910176 2307 100-101 0.22570044773155828 2201 1 0.13247359522672753 2201 2 0.11126911523828653 2201 3 0.11181808475665633 2201 4 0.12728190893599134 2201 5 0.14348755031186755 2201 6 0.09615106940130147 2201 7 0.20388259123174635 2201 8 0.1478877761056907 2201 9 0.28797945473407793 2201 10-11 0.2718962739730344 2201 12-13 0.24172564426366705 2201 14-15 0.429560887109254 2201 16-17 0.437940971965709 2201 18-19 0.40171365839693607 2201 20-21 0.4427146681210843 2201 22-23 0.4539320902200288 2201 24-25 0.3877236698617068 2201 26-27 0.38898713143119323 2201 28-29 0.32829005037076797 2201 30-31 0.4746457293406152 2201 32-33 0.39629925440986113 2201 34-35 0.507866053989396 2201 36-37 0.44656070253940783 2201 38-39 0.5146647433883871 2201 40-41 0.5194021287820902 2201 42-43 0.38104547335334615 2201 44-45 0.4865888032691785 2201 46-47 0.5203660064297466 2201 48-49 0.41346950742806854 2201 50-51 0.5291173806920639 2201 52-53 0.49763785395089855 2201 54-55 0.6134289154045334 2201 56-57 0.580400013916595 2201 58-59 0.604082239046221 2201 60-61 0.5973226811765571 2201 62-63 0.4986641030722936 2201 64-65 0.5595085812148639 2201 66-67 0.7153146074863841 2201 68-69 0.728447460453225 2201 70-71 0.5424645503245458 2201 72-73 0.06634295530529855 2201 74-75 0.10098206930184617 2201 76-77 0.08754391966120778 2201 78-79 0.3111892151480049 2201 80-81 0.5583089003715891 2201 82-83 0.474977160442684 2201 84-85 0.5034181942310312 2201 86-87 0.5601884188107853 2201 88-89 0.37241295928693674 2201 90-91 0.46151776619730356 2201 92-93 0.266083905942196 2201 94-95 0.16287626029789593 2201 96-97 0.40542425200384713 2201 98-99 0.45115744565700666 2201 100-101 0.2589091258459 1101 1 0.10146305899756669 1101 2 0.04975887525661449 1101 3 0.05199376870654504 1101 4 0.09074271588868754 1101 5 0.09785695324945465 1101 6 0.16448207187733743 1101 7 0.16953449831608935 1101 8 0.1603828445395692 1101 9 0.2000402625352109 1101 10-11 0.14514707044747865 1101 12-13 0.20866877323101107 1101 14-15 0.2748180638636555 1101 16-17 0.27070590842451026 1101 18-19 0.22719083602805767 1101 20-21 0.3209645525209339 1101 22-23 0.32754541867010545 1101 24-25 0.323812163722522 1101 26-27 0.27343983011144957 1101 28-29 0.22869450949639258 1101 30-31 0.4652533053574359 1101 32-33 0.3138457666790302 1101 34-35 0.3779537545574314 1101 36-37 0.4037713238955334 1101 38-39 0.39612085280339926 1101 40-41 0.5651679462059747 1101 42-43 0.40937634856316407 1101 44-45 0.6282537848367795 1101 46-47 0.579094772414031 1101 48-49 0.545738529839646 1101 50-51 0.5212678828987194 1101 52-53 0.3978553024328235 1101 54-55 0.5857796215199613 1101 56-57 0.6120972574716248 1101 58-59 0.4739440420692205 1101 60-61 0.4683673530347434 1101 62-63 0.4446724687053205 1101 64-65 0.42350588599908434 1101 66-67 0.467520732102642 1101 68-69 0.5298343794652425 1101 70-71 0.44568495138283026 1101 72-73 0.06078725441485844 1101 74-75 0.20389466286019697 1101 76-77 -0.01565078069046777 1101 78-79 0.43344662805195 1101 80-81 0.5677386204283188 1101 82-83 0.4144981303809878 1101 84-85 0.3872693832510663 1101 86-87 0.497857311693636 1101 88-89 0.36580306985300126 1101 90-91 0.40644507597318835 1101 92-93 0.518967192917632 1101 94-95 0.4163838587984152 1101 96-97 0.5428813173295985 1101 98-99 0.4891425243994476 1101 100-101 0.3374094065386224 2304 1 -0.03398002452878046 2304 2 -0.004781553170872144 2304 3 0.05456904448875122 2304 4 0.11050089121157924 2304 5 0.11039244834884698 2304 6 0.10668361861309705 2304 7 0.07663777106239422 2304 8 0.15686637612202503 2304 9 0.12143939102420376 2304 10-11 0.1010680455976356 2304 12-13 0.08621297035476516 2304 14-15 0.22586557822526743 2304 16-17 0.22785187343321667 2304 18-19 0.30834670040733414 2304 20-21 0.2543291280846063 2304 22-23 0.37050822896598135 2304 24-25 0.3577242143905508 2304 26-27 0.32048097825523314 2304 28-29 0.2571640327824838 2304 30-31 0.17243460189363446 2304 32-33 0.21551976136621676 2304 34-35 0.2688444361941933 2304 36-37 0.30742813699175287 2304 38-39 0.213206427071583 2304 40-41 0.27636869471092496 2304 42-43 0.36118888863827436 2304 44-45 0.4658977280969694 2304 46-47 0.32352155109472136 2304 48-49 0.41000494264275034 2304 50-51 0.3947579508137622 2304 52-53 0.3182152587264184 2304 54-55 0.2498755924172471 2304 56-57 0.22892978603128 2304 58-59 0.34518123439050186 2304 60-61 0.38018329540509654 2304 62-63 0.3296294165847726 2304 64-65 0.26669704264860883 2304 66-67 0.40904639980308133 2304 68-69 0.5587368775350896 2304 70-71 0.4922365288701016 2304 72-73 0.2655666613686307 2304 74-75 0.31262171372451064 2304 76-77 0.29540079025392885 2304 78-79 0.5255515038027454 2304 80-81 0.4965045359728819 2304 82-83 0.6347215041492937 2304 84-85 0.7006189756299293 2304 86-87 0.6377953506630014 2304 88-89 0.46127360074191515 2304 90-91 0.6217508245435717 2304 92-93 0.6317544251504508 2304 94-95 0.5513758110616003 2304 96-97 0.7004407920675568 2304 98-99 0.806635065521423 2304 100-101 0.6830691901003014 2202 1 0.0360779810833165 2202 2 0.07459504355401236 2202 3 0.08028835596003603 2202 4 0.11503663690827892 2202 5 0.12407297772858072 2202 6 0.12702537008941306 2202 7 0.10357687564002305 2202 8 0.03414053116663496 2202 9 0.14394221801251206 2202 10-11 0.1665882114476247 2202 12-13 0.1380557479231328 2202 14-15 0.1241966080866419 2202 16-17 0.031652078651625004 2202 18-19 0.14966679924722115 2202 20-21 0.2024230050373177 2202 22-23 0.15891234171045454 2202 24-25 0.13926816882368342 2202 26-27 0.1748277345479039 2202 28-29 0.16917820960537 2202 30-31 0.10367544035742782 2202 32-33 0.16548939741765167 2202 34-35 0.22373093784850795 2202 36-37 0.2339829805650666 2202 38-39 0.23516950869824882 2202 40-41 0.20909684313828336 2202 42-43 0.22010384864162091 2202 44-45 0.10648333349127626 2202 46-47 0.16207748358205265 2202 48-49 0.3258287479784272 2202 50-51 0.2100315404607045 2202 52-53 0.1274998212761531 2202 54-55 0.1973292088682257 2202 56-57 0.33736363450470463 2202 58-59 0.3351831190493755 2202 60-61 0.18762612741917195 2202 62-63 0.31730270049816767 2202 64-65 0.1353478362994096 2202 66-67 0.057396276253221856 2202 68-69 0.2799602305526392 2202 70-71 0.20152685009973936 2202 72-73 0.18846893133554232 2202 74-75 0.1206718247099765 2202 76-77 0.18305418054584166 2202 78-79 0.2144178814883837 2202 80-81 0.33156180866208373 2202 82-83 0.2013756064234471 2202 84-85 0.14206524191128622 2202 86-87 0.09877139455461403 2202 88-89 0.10075772772604452 2202 90-91 0.10616678969832094 2202 92-93 0.2162180302934793 2202 94-95 -0.19921471538645008 2202 96-97 0.016304908162542375 2202 98-99 0.16965648781065212 2202 100-101 0.3834220401233388 1102 1 0.07096411085252896 1102 2 0.024109726583745328 1102 3 0.06939707709782539 1102 4 0.05287169699164451 1102 5 0.10745751488488509 1102 6 0.13875387507717818 1102 7 0.05169433544666191 1102 8 0.10449854434567385 1102 9 0.13952483240134228 1102 10-11 0.13081770446191854 1102 12-13 0.06337028931957889 1102 14-15 0.21496439678771395 1102 16-17 0.10675933526683679 1102 18-19 0.1156989040857681 1102 20-21 0.13504956590459471 1102 22-23 0.1299232832561259 1102 24-25 0.10280632021842706 1102 26-27 0.07318396858680387 1102 28-29 0.07767518238070181 1102 30-31 0.029288951519838236 1102 32-33 0.06715867066493075 1102 34-35 -0.012922880088176214 1102 36-37 -0.017060159428943678 1102 38-39 0.006808674485363042 1102 40-41 0.025193671676973395 1102 42-43 0.074762445663346 1102 44-45 -0.06083054709750968 1102 46-47 0.10222710140203617 1102 48-49 -0.049296399740889285 1102 50-51 0.0066850177463422256 1102 52-53 0.033792186289012704 1102 54-55 0.0587596995746793 1102 56-57 0.1310317068877893 1102 58-59 0.10079947727913918 1102 60-61 -0.08577092243192652 1102 62-63 -0.05185274522435179 1102 64-65 -0.19993609358408548 1102 66-67 -0.10857777943020608 1102 68-69 0.0885480574564852 1102 70-71 -0.20035616091215047 1102 72-73 -0.26343439411112257 1102 74-75 -0.3011521511974067 1102 76-77 -0.24111239449584687 1102 78-79 -0.4030789674893711 1102 80-81 -0.26641386552928026 1102 82-83 -0.4170086662436283 1102 84-85 -0.5828725338810514 1102 86-87 -0.5749826603699688 1102 88-89 -0.498066666102023 1102 90-91 -0.4627184615789126 1102 92-93 -0.492287624929439 1102 94-95 -0.706358347995117 1102 96-97 -0.42262916699615616 1102 98-99 -0.2943511665908254 1102 100-101 -0.3229875725508755 2305 1 0.07110349682655936 2305 2 0.0648944552208448 2305 3 0.06837975580569378 2305 4 0.03745730968919503 2305 5 0.10553752217222012 2305 6 0.0913766479445286 2305 7 0.03164212525627619 2305 8 0.08351107390139134 2305 9 0.10765242985569046 2305 10-11 0.08082401282243268 2305 12-13 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0.19233930563391866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 13.0 10 17.0 11 18.0 12 45.0 13 94.0 14 317.0 15 790.0 16 1733.0 17 2842.0 18 3883.0 19 4903.0 20 6533.0 21 9158.0 22 12698.0 23 16962.0 24 22919.0 25 31030.0 26 42919.0 27 66096.0 28 120497.0 29 194432.0 30 139646.0 31 76405.0 32 91098.0 33 130586.0 34 200337.0 35 341650.0 36 589557.0 37 699268.0 38 106016.0 39 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.18036156795945 19.77145046843676 19.79948512546455 12.248702838139241 2 29.041870730454512 31.29372550204499 28.60753417696485 11.056869590535651 3 36.991603055531904 14.186084200175245 35.01755200364774 13.804760740645111 4 29.243145260445075 13.76271246979436 28.309123030193962 28.685019239566607 5 43.885777383460464 12.295363963731353 29.273013313702922 14.54584533910526 6 38.62264840232818 13.176363301754376 33.41548531702105 14.785502978896394 7 31.948526483199245 28.609525601478875 23.078378348683326 16.36356956663856 8 27.68251749020082 37.86322896437685 18.68756197450427 15.76669157091806 9 42.775729891419296 21.307212252891684 19.01793243439973 16.89912542128929 10-11 31.23870381611873 30.78146588071779 22.984094698415923 14.995735604747557 12-13 24.02068266032341 29.171536500751248 22.543989880816675 24.263790958108668 14-15 23.191923880888094 29.53663671747317 22.78617113615575 24.485268265482983 16-17 29.505666632789563 23.022515457574418 23.743514136367256 23.728303773268767 18-19 20.95179447949657 23.172919510741334 31.257313334853226 24.617972674908874 20-21 27.40521162662301 24.068717192997877 31.291854249699913 17.2342169306792 22-23 20.200804260824423 38.05622546771008 24.118914824713443 17.624055446752056 24-25 20.622505569121657 22.141172770497253 32.276356160094046 24.95996550028704 26-27 19.169624046519676 22.25507881920887 25.420654093682103 33.154643040589356 28-29 26.00239039611494 30.699714470926576 23.78571173512131 19.512183397837177 30-31 17.293118460917263 24.575225717668193 31.25243777828441 26.879218043130138 32-33 17.78714624746952 30.70467586475893 24.093644334328587 27.414533553442965 34-35 18.8923868527527 30.312399570401666 31.604044377178546 19.191169199667087 36-37 17.716914198444766 32.328510881418225 32.09731679581169 17.85725812432532 38-39 17.6642959558975 25.123279477889692 30.11893954584534 27.093485020367464 40-41 26.22263164689434 24.01070837030058 22.67101186339652 27.095648119408562 42-43 19.51074133180978 31.42162302471289 30.32007342176174 18.74756222171559 44-45 17.966425956089775 25.228224116288207 30.080364279612482 26.724985648009536 46-47 26.07006897025309 24.369409310769573 23.790814552852158 25.769707166125176 48-49 18.708179930486942 30.894922662647073 31.569949551959247 18.82694785490673 50-51 25.231953785209377 23.926437452521256 29.769930343048685 21.07167841922068 52-53 17.429655899029516 24.568146268964714 23.268366069333172 34.7338317626726 54-55 25.649798547813624 24.50900590080555 31.30898509200597 18.532210459374852 56-57 18.914529965892342 24.338016948235854 30.039310086097586 26.70814299977422 58-59 17.999960166025257 24.063807159607524 30.26654080046372 27.66969187390349 60-61 24.451516142057798 23.691539463094255 31.84291469005546 20.014029704792488 62-63 17.67714389456905 23.43483228337873 31.764773543719116 27.12325027833311 64-65 26.081465390841167 25.110860976180945 30.241833853786442 18.565839779191446 66-67 19.393947823029137 38.56031958862546 23.05441272447895 18.991319863866455 68-69 18.55137745971269 37.06164594561458 24.731718440963633 19.6552581537091 70-71 18.032517496866713 39.326761058196624 23.65107266666518 18.989648778271484 72-73 19.90617090370816 38.7241708566614 23.154852692989156 18.214805546641283 74-75 21.21016265739744 36.50195330710837 23.395177612633038 18.892706422861156 76-77 20.607817668435462 34.559963187540085 23.866905100603265 20.96531404342119 78-79 20.335509492956984 34.697802471365584 24.390184859683885 20.576503175993548 80-81 19.601554939377245 34.81617286657225 24.567671331839602 21.014600862210898 82-83 19.233482364885756 34.0023529852062 24.73824198391022 22.02592266599782 84-85 20.039187396306023 32.96318245745113 24.620177886675528 22.377452259567317 86-87 18.885702109627267 33.31870436818839 25.439272452800125 22.35632106938422 88-89 18.519463496789836 33.59709101348366 25.55534627402858 22.32809921569792 90-91 19.375804973566904 32.478220533868004 24.785158661245145 23.36081583131995 92-93 19.822319140442225 32.38805444781655 24.076014028339994 23.713612383401227 94-95 20.26147640192729 33.384352728201186 23.54570256608554 22.808468303785983 96-97 19.80198920846509 34.16372129377536 22.79342576286646 23.24086373489309 98-99 19.780738154985176 32.27873544417765 24.49727880327447 23.44324759756271 100-101 20.727558815004883 33.230666631868274 23.695609770838857 22.346164782287985 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1521.0 1 912.5 2 430.0 3 666.0 4 598.5 5 352.5 6 332.5 7 399.5 8 407.0 9 408.5 10 397.0 11 361.0 12 365.0 13 345.0 14 311.5 15 324.0 16 334.5 17 336.0 18 355.0 19 373.5 20 379.0 21 432.5 22 481.5 23 501.0 24 563.5 25 670.0 26 863.0 27 1175.0 28 1516.5 29 1964.5 30 2572.5 31 3665.0 32 6125.0 33 11002.5 34 19318.0 35 33659.0 36 55079.0 37 84602.5 38 123050.5 39 155485.5 40 173986.0 41 176213.5 42 191542.5 43 222371.5 44 212674.0 45 183106.0 46 176796.0 47 170703.0 48 147730.5 49 122357.5 50 100080.0 51 86976.5 52 82761.5 53 83440.5 54 74181.5 55 48059.0 56 29179.5 57 23731.0 58 22735.5 59 20035.0 60 15463.5 61 11640.0 62 7964.5 63 5012.5 64 3363.0 65 2353.5 66 1636.5 67 1230.5 68 896.5 69 653.5 70 529.0 71 396.0 72 254.5 73 174.0 74 127.0 75 79.0 76 45.5 77 32.0 78 26.0 79 20.5 80 12.5 81 8.0 82 7.0 83 7.0 84 5.0 85 3.5 86 2.0 87 0.0 88 1.5 89 2.5 90 2.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18444711188509616 2 0.0 3 0.0 4 0.026781226223078 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.716745270710128E-5 48-49 0.0029356344129143194 50-51 1.716745270710128E-5 52-53 0.0024892806425296857 54-55 0.008875573049571362 56-57 0.01323610603717509 58-59 0.01376829707109523 60-61 0.0032961509197634463 62-63 0.002077261777559255 64-65 0.009682443326805124 66-67 0.01697861072732317 68-69 0.024549457371154833 70-71 0.020927124849956465 72-73 0.01687560601108056 74-75 0.02168249276906892 76-77 0.014781176780814205 78-79 0.005871268825828639 80-81 0.0031416438453995346 82-83 6.008608447485449E-4 84-85 0.002472113189822585 86-87 0.01569105177429057 88-89 0.014025808861701749 90-91 0.017630973930193018 92-93 0.018042992795163446 94-95 0.017974322984335045 96-97 0.01977690551858068 98-99 0.027794105932796975 100-101 0.01634341497716042 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2912488.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.501873435867804 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12642270673818 18.652399742858723 2 9.77049839961573 4.787910303854671 3 3.9626816561297358 2.9127937321537747 4 2.0583140672637987 2.0173020306945557 5 1.6288962158311775 1.9955504460229756 6 0.9966534648097698 1.4651926232512864 7 0.6445089152077661 1.10541731080964 8 0.5959786253277488 1.1682074278610386 9 0.39320018167371834 0.8670726977696707 >10 3.0486057937067037 14.913460464465741 >50 0.40121147718009076 6.850378910953298 >100 0.3275991381850698 16.403567278357333 >500 0.03250834926547369 5.240075169204032 >1k 0.01152156535948478 5.1790313582805725 >5k 6.997218527995755E-4 1.191336757243274 >10k+ 6.997218525861417E-4 15.250303746219341 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 381833 13.110199939021209 TruSeq Adapter, Index 27 (97% over 39bp) GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 21021 0.7217540467119522 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 19492 0.6692559763336363 No Hit GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 11494 0.39464540283084426 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 10918 0.3748684973122636 TruSeq Adapter, Index 27 (97% over 39bp) TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT 8768 0.3010484506717281 No Hit GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC 7762 0.26650753582504033 No Hit TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 7594 0.2607392717154543 No Hit TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 5549 0.19052439014341005 No Hit TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT 5071 0.17411230535542122 No Hit GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC 4977 0.17088482424648616 No Hit GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA 4008 0.1376143009001239 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3914 0.13438681979118886 No Hit TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT 3878 0.13315076319627756 No Hit ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG 3862 0.13260140470965032 TruSeq Adapter, Index 27 (97% over 38bp) AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC 3747 0.128652890587017 No Hit GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA 3639 0.12494472080228312 No Hit GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC 3576 0.12278162176118837 No Hit CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC 3366 0.11557129162420583 TruSeq Adapter, Index 27 (97% over 36bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.007107325420739931 0.0 0.0 2 0.008000032961509198 0.0 0.0 3 0.008583726353550642 0.0 0.0 4 0.008892740502278465 0.0 0.0 5 0.010506481056745984 0.0 0.0 6 0.01136485369210105 0.0 0.0 7 0.012978594246568569 0.0 0.0 8 0.01445499517937928 0.0 0.0 9 0.016240410260917812 0.0 0.0 10-11 0.025047313499660773 0.0 0.0 12-13 0.03291000683951316 0.0 0.0 14-15 0.041184719044335974 0.0 0.0 16-17 0.05141652085776834 0.0 0.0 18-19 0.061528150502250994 0.0 0.0 20-21 0.06779427074034297 0.0 0.0 22-23 0.07345953013368639 0.0 0.0 24-25 0.07998316216238488 0.0 0.0 26-27 0.08654112909649757 0.0 0.0 28-29 0.09423214790927895 0.0 0.0 30-31 0.17280757894968152 0.0 0.0 32-33 0.18432693971614647 0.0 0.0 34-35 0.19316817786030363 0.0 0.0 36-37 0.20279911882898743 0.0 0.0 38-39 0.21515968477810038 0.0 0.0 40-41 0.23050738749824892 0.0 0.0 42-43 0.24659329068480282 0.0 0.0 44-45 0.2635375665067118 0.0 0.0 46-47 0.28206124797767407 0.0 0.0 48-49 0.3029712053749234 0.0 0.0 50-51 0.32461936323857815 0.0 0.0 52-53 0.3482761130689637 0.0 0.0 54-55 0.3755380279678405 0.0 0.0 56-57 0.40597592161753115 0.0 0.0 58-59 0.4408601855183609 0.0 0.0 60-61 0.47588178904084755 0.0 0.0 62-63 0.5140107015033195 0.0 0.0 64-65 0.5554700997909691 0.0 0.0 66-67 0.5994016112684413 0.0 0.0 68-69 0.6460284128209284 0.0 0.0 70-71 0.7008612567674098 0.0 0.0 72-73 0.7591275912553117 0.0 0.0 74-75 0.8231278549473853 0.0 0.0 76-77 0.8954886681078171 0.0 0.0 78-79 0.9756606722499801 0.0 0.0 80-81 1.0661331480164038 0.0 0.0 82-83 1.1683481614344848 0.0 0.0 84-85 1.2859623799308357 0.0 0.0 86-87 1.4151817964571871 0.0 0.0 88-89 1.5579635006221486 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 49935 0.0 83.060486 3 GATCGGA 50215 0.0 83.00861 1 CGGAAGA 49960 0.0 82.88581 4 GAAGAGC 50065 0.0 82.59202 6 ATCGGAA 51080 0.0 81.2544 2 GGAAGAG 51920 0.0 79.8058 5 AGAGCAC 58390 0.0 70.66999 8 GAGCACA 58510 0.0 70.63057 9 ATCGGAC 175 0.0 70.57016 2 AAGAGCA 58610 0.0 70.38042 7 TGTCGCG 25 0.005022406 56.99897 2 GTACGGG 175 0.0 54.393074 1 CGAGCAC 220 0.0 53.966103 8 TTGTGTT 23990 0.0 52.652905 1 TCGGGAA 145 0.0 52.412846 3 CGAGGTG 350 0.0 51.67342 1 CGTGGCA 140 0.0 47.593937 1 ATCGGAT 40 5.3900294E-4 47.499146 2 CGACGGG 195 0.0 46.373585 1 TTCCCTA 525 0.0 46.133312 8 >>END_MODULE