FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_50330665-1.341000000356c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_50330665-1.341000000356c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912488
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT38183313.110199939021209TruSeq Adapter, Index 27 (97% over 39bp)
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC210210.7217540467119522No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA194920.6692559763336363No Hit
GTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC114940.39464540283084426No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT109180.3748684973122636TruSeq Adapter, Index 27 (97% over 39bp)
TTGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACT87680.3010484506717281No Hit
GTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTTC77620.26650753582504033No Hit
TGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT75940.2607392717154543No Hit
TTGTGTTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT55490.19052439014341005No Hit
TGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGTT50710.17411230535542122No Hit
GAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGC49770.17088482424648616No Hit
GTGTTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATA40080.1376143009001239No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39140.13438681979118886No Hit
TTGTGTTTGAGGGGGGTGACGGGCGGTGTGTACGCGCTTCAGGGCCCTGT38780.13315076319627756No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG38620.13260140470965032TruSeq Adapter, Index 27 (97% over 38bp)
AGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCC37470.128652890587017No Hit
GTGTTTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAA36390.12494472080228312No Hit
GGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTACGATAGCCC35760.12278162176118837No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATGCC33660.11557129162420583TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG499350.083.0604863
GATCGGA502150.083.008611
CGGAAGA499600.082.885814
GAAGAGC500650.082.592026
ATCGGAA510800.081.25442
GGAAGAG519200.079.80585
AGAGCAC583900.070.669998
GAGCACA585100.070.630579
ATCGGAC1750.070.570162
AAGAGCA586100.070.380427
TGTCGCG250.00502240656.998972
GTACGGG1750.054.3930741
CGAGCAC2200.053.9661038
TTGTGTT239900.052.6529051
TCGGGAA1450.052.4128463
CGAGGTG3500.051.673421
CGTGGCA1400.047.5939371
ATCGGAT405.3900294E-447.4991462
CGACGGG1950.046.3735851
TTCCCTA5250.046.1333128
Produced by FastQC (version 0.11.2)