##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_50220371-2.34100000035905.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3477869 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.681587201818125 33.0 33.0 33.0 33.0 33.0 2 32.72535308259167 33.0 33.0 33.0 33.0 33.0 3 32.75633383546074 33.0 33.0 33.0 33.0 33.0 4 36.686093409498746 37.0 37.0 37.0 37.0 37.0 5 36.657496875241705 37.0 37.0 37.0 37.0 37.0 6 36.52629469367593 37.0 37.0 37.0 37.0 37.0 7 36.47693314498045 37.0 37.0 37.0 37.0 37.0 8 36.40407933708831 37.0 37.0 37.0 37.0 37.0 9 36.403275684046754 37.0 37.0 37.0 37.0 37.0 10-11 36.35515368750232 37.0 37.0 37.0 37.0 37.0 12-13 36.34854403659253 37.0 37.0 37.0 37.0 37.0 14-15 38.211258388398186 40.0 37.0 40.0 37.0 40.0 16-17 38.04907171604221 40.0 37.0 40.0 37.0 40.0 18-19 37.82784400447515 40.0 37.0 40.0 33.0 40.0 20-21 37.71041620601581 40.0 37.0 40.0 33.0 40.0 22-23 37.548362517392114 40.0 37.0 40.0 33.0 40.0 24-25 37.47010755724267 40.0 37.0 40.0 33.0 40.0 26-27 37.32835926252541 40.0 37.0 40.0 33.0 40.0 28-29 37.15449359938514 37.0 37.0 40.0 33.0 40.0 30-31 37.07801832098909 37.0 37.0 40.0 33.0 40.0 32-33 36.947097202338554 37.0 37.0 40.0 33.0 40.0 34-35 36.817084973585835 37.0 37.0 40.0 33.0 40.0 36-37 36.69786024142945 37.0 37.0 40.0 33.0 40.0 38-39 36.59528751657984 37.0 37.0 40.0 33.0 40.0 40-41 36.462702447964546 37.0 37.0 40.0 33.0 40.0 42-43 36.3086195886044 37.0 37.0 40.0 33.0 40.0 44-45 36.13738786596045 37.0 37.0 40.0 33.0 40.0 46-47 36.0385176957499 37.0 37.0 40.0 33.0 40.0 48-49 35.80209116559594 37.0 37.0 40.0 27.0 40.0 50-51 35.611421821811 37.0 37.0 40.0 27.0 40.0 52-53 35.42499616863085 37.0 37.0 40.0 27.0 40.0 54-55 35.220287624404484 37.0 33.0 40.0 27.0 40.0 56-57 35.012817044000215 37.0 33.0 40.0 27.0 40.0 58-59 34.750024512136605 37.0 33.0 40.0 27.0 40.0 60-61 34.4852996188183 37.0 33.0 40.0 27.0 40.0 62-63 34.16519109834212 37.0 33.0 37.0 27.0 40.0 64-65 33.9116378448987 37.0 33.0 37.0 27.0 40.0 66-67 33.60698879112468 37.0 33.0 37.0 22.0 40.0 68-69 33.32089247754875 37.0 33.0 37.0 22.0 40.0 70-71 32.98625451389917 37.0 33.0 37.0 22.0 38.5 72-73 32.69592126672971 37.0 33.0 37.0 22.0 37.0 74-75 32.37602034464208 37.0 33.0 37.0 22.0 37.0 76-77 29.609454237638047 33.0 27.0 35.0 18.5 37.0 78-79 31.991739338083175 35.0 33.0 37.0 22.0 37.0 80-81 32.337711109877915 37.0 33.0 37.0 22.0 37.0 82-83 32.204013865962175 37.0 33.0 37.0 22.0 37.0 84-85 31.967903477675552 37.0 33.0 37.0 22.0 37.0 86-87 31.812030154097236 33.0 33.0 37.0 18.5 37.0 88-89 31.622059801562393 33.0 33.0 37.0 15.0 37.0 90-91 31.41609430947514 33.0 33.0 37.0 15.0 37.0 92-93 31.23881879967302 33.0 33.0 37.0 15.0 37.0 94-95 31.071274104918842 33.0 33.0 37.0 15.0 37.0 96-97 30.858422787057247 33.0 33.0 37.0 15.0 37.0 98-99 30.619810723175597 33.0 27.0 37.0 15.0 37.0 100-101 29.003531329098365 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10130764411228199 2306 2 0.09417847282671232 2306 3 0.12023522638197903 2306 4 0.1641505345519363 2306 5 0.19335641788332936 2306 6 0.22329711918112594 2306 7 0.19463952427960152 2306 8 0.247000850357189 2306 9 0.2258848346784319 2306 10-11 0.25624805239406356 2306 12-13 0.2509857996285305 2306 14-15 0.4901626671064534 2306 16-17 0.533381258403935 2306 18-19 0.5363685278744086 2306 20-21 0.7247875442927025 2306 22-23 0.7486313644471778 2306 24-25 0.6375592805268298 2306 26-27 0.8281913408327668 2306 28-29 0.7480894896633217 2306 30-31 0.6379258285407943 2306 32-33 0.7215753697789822 2306 34-35 0.672673419427305 2306 36-37 0.6883562576085467 2306 38-39 0.688900987693799 2306 40-41 0.6564427511816717 2306 42-43 0.7158589036111636 2306 44-45 0.7319595582189962 2306 46-47 0.7745648865090828 2306 48-49 0.8570090598862095 2306 50-51 0.7161960215362342 2306 52-53 0.6888225460092343 2306 54-55 0.8040917663551852 2306 56-57 0.7657728889763575 2306 58-59 0.7188451932272386 2306 60-61 0.6670347397927827 2306 62-63 0.6338007451852334 2306 64-65 0.6720311555723981 2306 66-67 0.6771731945277608 2306 68-69 0.911240130008693 2306 70-71 0.8163535957656123 2306 72-73 0.6003256153129826 2306 74-75 0.5994959372871591 2306 76-77 0.6080594241099995 2306 78-79 0.6595026211303221 2306 80-81 0.813232657388788 2306 82-83 0.8448826761101316 2306 84-85 0.9526230886175711 2306 86-87 0.8977006511663852 2306 88-89 0.9112447620027204 2306 90-91 0.7287269495589115 2306 92-93 1.0643933911888688 2306 94-95 1.0543458210962449 2306 96-97 1.1595760008159601 2306 98-99 1.2432312101848133 2306 100-101 1.0998436073141775 2307 1 0.14110098067421717 2307 2 0.09990471048472216 2307 3 0.10302721596762154 2307 4 0.1453902892723491 2307 5 0.1710991877692365 2307 6 0.15974549991513243 2307 7 0.1524990391704648 2307 8 0.18546069450892588 2307 9 0.2303281621334463 2307 10-11 0.15260844550216746 2307 12-13 0.13044201089766716 2307 14-15 0.36495829098661403 2307 16-17 0.4200629067697079 2307 18-19 0.5217224550727408 2307 20-21 0.5906460369499982 2307 22-23 0.5957840591763244 2307 24-25 0.5558969014052408 2307 26-27 0.4918536373567477 2307 28-29 0.4173246535101427 2307 30-31 0.5101398361058855 2307 32-33 0.5257466016692831 2307 34-35 0.49262123468540864 2307 36-37 0.47647487482583983 2307 38-39 0.5611016702182638 2307 40-41 0.5347568917500922 2307 42-43 0.4676147203884966 2307 44-45 0.5053436808418397 2307 46-47 0.4539510839554808 2307 48-49 0.4757199936909302 2307 50-51 0.6038889610648965 2307 52-53 0.41066364657640264 2307 54-55 0.40944597946829475 2307 56-57 0.4852720474691594 2307 58-59 0.6060233979083307 2307 60-61 0.6549074526510381 2307 62-63 0.5287643034052465 2307 64-65 0.40791949632068736 2307 66-67 0.5258494443989221 2307 68-69 0.5654249693245887 2307 70-71 0.6209311972724123 2307 72-73 0.43303564266197014 2307 74-75 0.4720432473585561 2307 76-77 0.6606952759877878 2307 78-79 0.5726643903003819 2307 80-81 0.491280438337121 2307 82-83 0.6240623089531923 2307 84-85 0.5831415968342668 2307 86-87 0.6580047021199285 2307 88-89 0.5252110106455099 2307 90-91 0.698436749587998 2307 92-93 0.9405291082278318 2307 94-95 0.6717564603734552 2307 96-97 0.8478902402191153 2307 98-99 0.9901047673741203 2307 100-101 0.9988490651096242 2201 1 0.1012970407556324 2201 2 0.10709319020022434 2201 3 0.061221731900431564 2201 4 0.08824743212949215 2201 5 0.08928036787311555 2201 6 0.07509691070545443 2201 7 0.12488568462458716 2201 8 0.21470593338403177 2201 9 0.12362281376416462 2201 10-11 0.14041142617691804 2201 12-13 0.13390464718048634 2201 14-15 0.26127690031702144 2201 16-17 0.29081006413247934 2201 18-19 0.4284640675382363 2201 20-21 0.5002279470150341 2201 22-23 0.5326816072554976 2201 24-25 0.46003685742040545 2201 26-27 0.4133974350264893 2201 28-29 0.47289587151585266 2201 30-31 0.38291544323704585 2201 32-33 0.48462019204876583 2201 34-35 0.6062518556203216 2201 36-37 0.4306078119836698 2201 38-39 0.4688294321386266 2201 40-41 0.5046331258991756 2201 42-43 0.37414752184842825 2201 44-45 0.5998233495517766 2201 46-47 0.524065374349 2201 48-49 0.46137695873702 2201 50-51 0.5160169616264056 2201 52-53 0.5425304286642856 2201 54-55 0.5496704659218992 2201 56-57 0.6466700868683404 2201 58-59 0.5813322276308526 2201 60-61 0.581808978082698 2201 62-63 0.7153594557144416 2201 64-65 0.5897558633601321 2201 66-67 0.6229155620349687 2201 68-69 0.5543246464571538 2201 70-71 0.5270235654348738 2201 72-73 0.538916275294973 2201 74-75 0.6205926835103526 2201 76-77 0.6893041384649798 2201 78-79 0.797387730340624 2201 80-81 0.8239263135886361 2201 82-83 0.7141522673079734 2201 84-85 0.9369259307447066 2201 86-87 1.0048029333678805 2201 88-89 0.8682804739368883 2201 90-91 0.9045607026084497 2201 92-93 1.0175376712337787 2201 94-95 0.9709814615432641 2201 96-97 1.1023951768154348 2201 98-99 0.92895823702926 2201 100-101 0.8341825778137597 1101 1 0.07912472524238012 1101 2 0.040371115297617166 1101 3 0.07503191435549894 1101 4 0.06241632781157591 1101 5 0.08855780552154613 1101 6 0.052660880692748435 1101 7 0.10840468167913286 1101 8 0.11192032404592567 1101 9 0.11918164631622119 1101 10-11 0.11726905648301766 1101 12-13 0.08614310659574187 1101 14-15 0.2593402517669503 1101 16-17 0.2870767740596065 1101 18-19 0.3306806532581348 1101 20-21 0.35048062856093765 1101 22-23 0.49499349924172975 1101 24-25 0.4794444762322385 1101 26-27 0.5599354325953882 1101 28-29 0.4575995079312136 1101 30-31 0.47040515029868146 1101 32-33 0.5883437177655466 1101 34-35 0.5079370143372373 1101 36-37 0.5593880593832452 1101 38-39 0.5554444989245084 1101 40-41 0.6312253886849035 1101 42-43 0.5892614380548693 1101 44-45 0.5604972131383406 1101 46-47 0.5143499169119821 1101 48-49 0.5701189021821804 1101 50-51 0.5242243257961405 1101 52-53 0.6010992662215315 1101 54-55 0.6006244896438986 1101 56-57 0.54206600075441 1101 58-59 0.5847452929543948 1101 60-61 0.5600144739543751 1101 62-63 0.637372312372932 1101 64-65 0.5249512158341716 1101 66-67 0.6732907208979597 1101 68-69 0.8006669724805491 1101 70-71 0.679949220242591 1101 72-73 0.6264225125657461 1101 74-75 0.765659800003732 1101 76-77 0.6007175174780706 1101 78-79 0.8046264242541952 1101 80-81 0.7460364293846666 1101 82-83 0.7009277155649656 1101 84-85 0.861100400567075 1101 86-87 0.9834088447244476 1101 88-89 0.8340447193251457 1101 90-91 1.0414490560381608 1101 92-93 1.0657793567307827 1101 94-95 1.0572801478144989 1101 96-97 1.089194382442784 1101 98-99 1.1826898740812233 1101 100-101 0.8994862070019565 2304 1 0.027015453797993416 2304 2 0.00891953287163716 2304 3 0.027609803922302945 2304 4 0.13586647974854316 2304 5 0.0720745689771718 2304 6 0.10536054378599147 2304 7 0.0474879649733424 2304 8 0.12432260778944482 2304 9 0.05305582207041226 2304 10-11 0.1279417362424411 2304 12-13 0.08456501967184238 2304 14-15 0.07831760609011695 2304 16-17 0.2964567648496086 2304 18-19 0.3720099582672205 2304 20-21 0.3395407468912168 2304 22-23 0.3802748590741061 2304 24-25 0.4131142964133332 2304 26-27 0.36458662142693754 2304 28-29 0.3632069688691999 2304 30-31 0.34422174257151283 2304 32-33 0.3402101420049135 2304 34-35 0.39608831024550994 2304 36-37 0.4072419759417798 2304 38-39 0.40430381690877937 2304 40-41 0.3891375885726518 2304 42-43 0.39469645648186713 2304 44-45 0.5281032855781902 2304 46-47 0.4268203658363845 2304 48-49 0.37133929686837064 2304 50-51 0.4172706375279702 2304 52-53 0.35572498059122637 2304 54-55 0.45573505860620855 2304 56-57 0.2582627195877407 2304 58-59 0.306924241251032 2304 60-61 0.34660286963715237 2304 62-63 0.3582578718522953 2304 64-65 0.2726119743852635 2304 66-67 0.3688939967457969 2304 68-69 0.4768675964648921 2304 70-71 0.3435225593986644 2304 72-73 0.23948636248477584 2304 74-75 0.47030119096534406 2304 76-77 0.3429723012880679 2304 78-79 0.5323255624615513 2304 80-81 0.5454013174580012 2304 82-83 0.4847244181618109 2304 84-85 0.5740202917827304 2304 86-87 0.5491304368503158 2304 88-89 0.6650302030809847 2304 90-91 0.6925269617014678 2304 92-93 0.8569742909520919 2304 94-95 0.7746006123455125 2304 96-97 0.9167866538829692 2304 98-99 1.0267294798709266 2304 100-101 0.7505964456360772 2202 1 0.01590989269243437 2202 2 0.01796049191259641 2202 3 0.003900180212681903 2202 4 0.0036320475141096153 2202 5 0.07378952069212374 2202 6 0.006276294701741847 2202 7 0.0031489706343492685 2202 8 0.02748611095294251 2202 9 0.021470740485327156 2202 10-11 0.03078056408126173 2202 12-13 0.08891899902582168 2202 14-15 0.05743848521100148 2202 16-17 0.05968520307804681 2202 18-19 0.23866829992556404 2202 20-21 0.21144384379431358 2202 22-23 0.26176836556760463 2202 24-25 0.31135771965676184 2202 26-27 0.25009278193309825 2202 28-29 0.23128888695111982 2202 30-31 0.3720688954186713 2202 32-33 0.31654214333691755 2202 34-35 0.4225285366857321 2202 36-37 0.2979928916926937 2202 38-39 0.41057254817751243 2202 40-41 0.4344089838440439 2202 42-43 0.44203245381785905 2202 44-45 0.36450022197512766 2202 46-47 0.5476495366655598 2202 48-49 0.5629560134850919 2202 50-51 0.4537341739915064 2202 52-53 0.4849957098619555 2202 54-55 0.5317257345968827 2202 56-57 0.47060038192540077 2202 58-59 0.4193368286636243 2202 60-61 0.4067094297437137 2202 62-63 0.5522472158416463 2202 64-65 0.33916209093538185 2202 66-67 0.4863848392366421 2202 68-69 0.4035159481132382 2202 70-71 0.3069091727852751 2202 72-73 0.36015735815577443 2202 74-75 0.42795187361602416 2202 76-77 0.5573079656237319 2202 78-79 0.6185809737169663 2202 80-81 0.6688029158596009 2202 82-83 0.6617557201931206 2202 84-85 0.640736908499342 2202 86-87 0.7002293379492208 2202 88-89 0.7736216116723931 2202 90-91 0.6805389497134513 2202 92-93 0.9412150501928487 2202 94-95 0.5919998797447796 2202 96-97 0.772322784419103 2202 98-99 0.8278533559948009 2202 100-101 0.840706335745967 1102 1 0.014639291405238453 1102 2 0.052846034514701046 1102 3 0.09526255166134234 1102 4 0.10271805941127354 1102 5 0.05523994879040828 1102 6 0.12028099229422651 1102 7 0.08886738431493058 1102 8 0.11183180177059171 1102 9 0.1420462651929455 1102 10-11 0.11194998377541054 1102 12-13 0.10752966980210488 1102 14-15 0.1403765229449192 1102 16-17 0.21809845428087726 1102 18-19 0.3051329089972086 1102 20-21 0.18938993520704628 1102 22-23 0.39344525986481926 1102 24-25 0.18378692503485894 1102 26-27 0.14355334559778044 1102 28-29 0.435848963449466 1102 30-31 0.3266068649168119 1102 32-33 0.4729301380173112 1102 34-35 0.3317215673523748 1102 36-37 0.21655923242133213 1102 38-39 0.36567195424770915 1102 40-41 0.2748514487803675 1102 42-43 0.3352535539921675 1102 44-45 0.3539999190918124 1102 46-47 0.38045388797306856 1102 48-49 0.3018385133284269 1102 50-51 0.1933865892828237 1102 52-53 0.3216869475986641 1102 54-55 0.3677543425637637 1102 56-57 0.4002394285740678 1102 58-59 0.3562170493390582 1102 60-61 0.06035366138329579 1102 62-63 0.219883379876741 1102 64-65 0.22454675273889535 1102 66-67 0.31834442462304224 1102 68-69 0.3394927409851576 1102 70-71 0.15441346073098572 1102 72-73 0.20387322575979283 1102 74-75 0.17570804210733826 1102 76-77 0.12464275496316901 1102 78-79 0.24786056402376033 1102 80-81 0.40213696481760763 1102 82-83 0.5326273394467549 1102 84-85 0.5699356718998985 1102 86-87 0.5044865311377116 1102 88-89 0.4675569826406196 1102 90-91 0.3972415136612888 1102 92-93 0.4798655715931197 1102 94-95 0.29586518138638596 1102 96-97 0.5779391809664602 1102 98-99 0.7087666594188917 1102 100-101 0.6035481238947611 2305 1 0.057495262055581975 2305 2 0.017507241459348677 2305 3 0.01055648686898536 2305 4 -0.02514288126081965 2305 5 0.059091255993863 2305 6 0.0027783523149125244 2305 7 -0.008216824064774642 2305 8 0.05960502084963082 2305 9 0.024249237708268367 2305 10-11 0.025581133881836138 2305 12-13 -0.0158804196624871 2305 14-15 -0.015021153915306229 2305 16-17 0.027843279569459867 2305 18-19 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1213 62-63 -0.3936975617197973 1213 64-65 -0.3387914957034752 1213 66-67 -0.2990224870506779 1213 68-69 -0.42076818071416966 1213 70-71 -0.29725983802428857 1213 72-73 -0.37464147095320044 1213 74-75 -0.3100622954749639 1213 76-77 -0.5005980878106975 1213 78-79 -0.5729005000930059 1213 80-81 -0.6783470629595953 1213 82-83 -0.8087162643482344 1213 84-85 -0.8430926481469534 1213 86-87 -0.7609203188148079 1213 88-89 -0.7522179032938219 1213 90-91 -0.7370540153188401 1213 92-93 -0.8599894829006622 1213 94-95 -0.8514183683552936 1213 96-97 -1.006045030643726 1213 98-99 -0.8220954960322082 1213 100-101 -0.4991303515491552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 30.0 9 96.0 10 139.0 11 151.0 12 189.0 13 519.0 14 1685.0 15 4099.0 16 7857.0 17 12197.0 18 15388.0 19 16861.0 20 19462.0 21 22214.0 22 25123.0 23 28844.0 24 33236.0 25 38013.0 26 43779.0 27 51123.0 28 59719.0 29 70378.0 30 83131.0 31 100869.0 32 126590.0 33 166940.0 34 241126.0 35 393407.0 36 742578.0 37 932047.0 38 240031.0 39 46.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.34137964511954 31.548511939829403 20.71510202388398 15.395006391167081 2 29.90377153366041 25.08645380260154 31.6964497512701 13.31332491246795 3 32.9179736212031 23.026054172828246 26.69525505417254 17.360717151796116 4 29.07542757516485 23.25306565360681 30.365824933110268 17.305681838118073 5 28.86767730469434 21.623356141361278 31.576807522077456 17.932159031866927 6 27.128106320278306 22.3478802680607 32.47045820299729 18.053555208663695 7 30.508222132576012 22.365793536214273 26.998026665179164 20.12795766603055 8 27.153265404763665 27.044836938941636 26.416147359201858 19.385750297092848 9 24.55716417150847 24.77865037469784 30.9736795721748 19.69050588161889 10-11 19.63455207772346 30.242614083509185 34.90741025610798 15.215423582659382 12-13 19.673886509238848 29.826123410628753 34.762091384120566 15.73789869601184 14-15 19.901065853831756 29.69276588623666 34.85883165812168 15.547336601809903 16-17 19.782142455624406 29.55700746635368 34.75812343708173 15.902726640940184 18-19 19.03373876359345 29.935285084055785 34.39936926894026 16.6316068834105 20-21 19.093272345795658 30.091760212934986 33.96832945691744 16.84663798435191 22-23 19.00620753685662 29.690839419196063 34.10477795454631 17.198175089401012 24-25 18.96723252083388 29.182352756817465 34.41104308414147 17.43937163820719 26-27 19.07731429792209 29.576142747182253 34.20762829192244 17.138914662973217 28-29 18.846195759529756 29.963477635299085 34.075291507529464 17.115035097641687 30-31 18.205286053039952 31.052779733796758 33.832384140978284 16.909550072185006 32-33 18.008168220252113 31.4187653416503 33.85952719898306 16.713539239114528 34-35 18.34942604221148 30.889461333937536 34.29477936057971 16.466333263271274 36-37 18.47205860830296 30.51873144158104 34.657501475760014 16.35170847435599 38-39 18.780063308882536 30.33755728004706 34.01496721124344 16.867412199826965 40-41 18.621503570145972 30.20652876804733 33.546778213900524 17.625189447906177 42-43 18.814466559838795 29.879043747766232 33.49953664154688 17.80695305084809 44-45 18.445102446354362 29.73418492760941 33.74044565795894 18.08026696807729 46-47 18.603997193105943 30.02457973144735 33.59232839797859 17.77909467746812 48-49 18.477255562915058 30.687525420599954 33.116976381647795 17.71824263483719 50-51 19.06487169570987 30.58960022887605 32.149853430193524 18.195674645220556 52-53 18.975581170331424 30.468846820387913 32.22413980004986 18.331432209230808 54-55 19.348562901948707 30.57524036944234 32.16482533558839 17.911371393020556 56-57 19.273094846786844 30.646283299471293 32.06024998957538 18.020371864166485 58-59 19.0516519710088 31.239677787360478 31.945175768426992 17.763494473203732 60-61 18.941246631074595 31.12835314648216 31.78436807111283 18.14603215133042 62-63 18.485846594887263 31.037168772652706 32.129458836587084 18.347525795872947 64-65 18.465422561061615 30.59118907982953 32.49504707631924 18.44834128278962 66-67 18.702876388913054 30.594558965059633 32.068248685122285 18.634315960905028 68-69 19.07842569362615 30.642030032997702 32.00080876433637 18.27873550903978 70-71 18.65311200598663 30.850595413050367 32.21674093392103 18.279551647041973 72-73 18.649172907248275 30.767347091371317 31.937053525209645 18.646426476170756 74-75 18.973142803485285 30.3814081690202 31.57143122143882 19.074017806055693 76-77 18.950065474960553 30.096338132913235 31.26056589863898 19.69303049348723 78-79 18.675359387052502 30.30193760248561 31.474290265835354 19.548412744626535 80-81 18.41060610003438 30.664488620393538 31.0640159563647 19.86088932320739 82-83 17.974696683534873 30.922774149333016 30.841358778404267 20.261170388727844 84-85 17.931801324729914 31.020866703059767 31.30650988400454 19.740822088205775 86-87 18.157470439800665 30.792330244270754 31.542200436923405 19.50799887900517 88-89 18.547314819248335 30.68616341105027 31.404795993211398 19.361725776489997 90-91 18.43220920864166 30.742689585050183 30.924877476126845 19.900223730181317 92-93 18.04033164901342 30.861462051035755 30.88537517578951 20.212831124161315 94-95 17.823138681450214 30.703563414115788 30.888755575967895 20.584542328466103 96-97 17.869239248817056 31.179358027844234 30.614207870918797 20.337194852419913 98-99 18.39855362062943 30.95438230256129 30.259379297659827 20.387684779149453 100-101 18.54857889586085 30.9216794482784 29.82146005146004 20.708281604400707 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 182196.0 1 99935.5 2 15260.5 3 13120.0 4 11844.5 5 9583.5 6 9060.0 7 9570.5 8 9698.5 9 9035.5 10 8111.0 11 7267.5 12 6557.5 13 6070.5 14 5733.5 15 5663.5 16 5671.0 17 5581.0 18 5678.5 19 5726.0 20 5806.5 21 6152.5 22 6678.5 23 7348.5 24 8387.0 25 9764.0 26 11582.0 27 14214.5 28 17524.0 29 21013.0 30 25605.5 31 31631.0 32 40560.0 33 56186.5 34 77074.5 35 99567.0 36 129278.0 37 168197.0 38 197459.5 39 200890.0 40 199554.5 41 198868.5 42 190226.0 43 184360.5 44 174177.0 45 161721.5 46 157278.5 47 145492.5 48 120689.5 49 97036.0 50 78066.0 51 63011.0 52 51342.0 53 40314.0 54 31937.5 55 26750.0 56 21282.5 57 15818.0 58 11516.0 59 8235.5 60 5896.5 61 4506.5 62 3472.0 63 2686.5 64 2148.0 65 1609.0 66 1098.0 67 775.0 68 609.5 69 482.0 70 372.0 71 285.5 72 229.0 73 170.5 74 139.5 75 106.5 76 85.0 77 74.5 78 56.0 79 48.0 80 41.0 81 26.0 82 22.0 83 14.5 84 11.0 85 12.0 86 7.5 87 6.0 88 4.0 89 3.0 90 2.5 91 2.5 92 2.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1918416133557647 2 0.0 3 0.0 4 0.025245344203591338 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.0063633794142333E-4 48-49 0.003694791264420828 50-51 1.1501295764734095E-4 52-53 0.002932830420007194 54-55 0.010279283089731098 56-57 0.015066697451801664 58-59 0.015469242803567356 60-61 0.0036804146447149103 62-63 0.00241527211059416 64-65 0.010854347877967803 66-67 0.018531462800927812 68-69 0.027603109835361826 70-71 0.02262879941711433 72-73 0.018085787590044363 74-75 0.02281569547329126 76-77 0.01683502167562953 78-79 0.007087673515017386 80-81 0.003867310700891839 82-83 7.044543655899632E-4 84-85 0.002975960279124947 86-87 0.01726632026680706 88-89 0.016403723084452003 90-91 0.019825358574460397 92-93 0.020098514348872828 94-95 0.01926467040592961 96-97 0.021277397164758075 98-99 0.03017652476272108 100-101 0.01748196956239582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3477869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.71052500888903 #Duplication Level Percentage of deduplicated Percentage of total 1 81.23098622298036 34.69418068573323 2 9.260076523094531 7.910054598477102 3 3.2944320981009128 4.221207735480773 4 1.5243584521862894 2.6042459917845555 5 0.8957580095474968 1.9129147434345506 6 0.6722525700629784 1.7227356123578859 7 0.41568823546663525 1.2427983943759073 8 0.3013968686150526 1.0298254795667234 9 0.3101611736243263 1.1922431906581343 >10 1.7866792153728617 14.32005882798347 >50 0.16999665344250348 5.01843808823788 >100 0.12510541473587689 11.144806009011834 >500 0.01086368900160141 2.9888656378769736 >1k 0.0021185780924236424 1.2126508356710897 >5k 0.0 0.0 >10k+ 1.2629567600192518E-4 8.784974169350008 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203348 5.846913727917872 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122374 3.5186489197839252 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT 3735 0.10739334920320459 TruSeq Adapter, Index 19 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.300259152946819E-4 0.0 0.0 2 2.300259152946819E-4 0.0 0.0 3 2.300259152946819E-4 0.0 0.0 4 2.587791547065171E-4 0.0 0.0 5 3.4503887294202285E-4 0.0 0.0 6 4.312985911775285E-4 0.0 0.0 7 5.750647882367048E-4 0.0 0.0 8 7.475842247077161E-4 0.0 0.0 9 9.201036611787276E-4 0.0 0.0 10-11 0.0022139994347113133 0.0 0.0 12-13 0.008769738020609746 0.0 0.0 14-15 0.022542539698878823 0.0 0.0 16-17 0.054099219953368 0.0 0.0 18-19 0.09282983344111007 0.0 0.0 20-21 0.10463303821966842 0.0 0.0 22-23 0.11356091905704327 0.0 0.0 24-25 0.12238816355647668 0.0 0.0 26-27 0.13342940749062143 0.0 0.0 28-29 0.1453332486071212 0.0 0.0 30-31 0.15797029732862278 0.0 0.0 32-33 0.17184373534483327 0.0 0.0 34-35 0.18755738068340125 0.0 0.0 36-37 0.20401861024667692 0.0 0.0 38-39 0.22141432009083722 0.0 0.0 40-41 0.2415415876791219 0.0 0.0 42-43 0.26080625808505153 0.0 0.0 44-45 0.2822417980665747 0.0 0.0 46-47 0.3045543118501588 0.0 0.0 48-49 0.32915270816698383 0.0 0.0 50-51 0.3550306236376356 0.0 0.0 52-53 0.3833669410779992 0.0 0.0 54-55 0.41268086865836523 0.0 0.0 56-57 0.44520078243315087 0.0 0.0 58-59 0.4800065787411774 0.0 0.0 60-61 0.5148842581477335 0.0 0.0 62-63 0.5516308981160589 0.0 0.0 64-65 0.5911953555467442 0.0 0.0 66-67 0.6344258510024385 0.0 0.0 68-69 0.6820555920881436 0.0 0.0 70-71 0.7351196954226855 0.0 0.0 72-73 0.7944520049490076 0.0 0.0 74-75 0.8591755468650487 0.0 0.0 76-77 0.932769463139641 0.0 0.0 78-79 1.016369506729552 0.0 0.0 80-81 1.1117871317177273 0.0 0.0 82-83 1.218921701766225 0.0 0.0 84-85 1.3408641901118186 0.0 0.0 86-87 1.4817550632298113 0.0 1.4376619705917618E-5 88-89 1.6426869442178529 2.8753239411835237E-5 2.8753239411835237E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCGT 20 0.0020575717 71.396736 1 CGCGAGG 80 4.152753E-9 47.597824 1 AACGGTG 60 1.4721481E-6 47.597824 1 TGTACCG 40 5.3870457E-4 47.504738 2 ATCGCGG 45 9.530779E-4 42.309177 1 GTCGAGC 115 5.4569682E-11 41.29837 7 CGGACGG 70 4.252177E-6 40.798138 1 CGGCACC 105 1.027729E-9 40.71835 3 CGACGGG 165 0.0 40.386036 1 CTCGCGG 50 0.0016000124 38.07826 1 CGGTGCG 65 1.3127516E-4 36.613712 1 CGTACCG 65 1.3279585E-4 36.542107 2 TCGCGGC 65 1.3279585E-4 36.542107 2 CGGTCAG 105 4.5785782E-8 36.26501 1 ACTGGCG 305 0.0 35.823246 2 CGACGCC 95 8.5676584E-7 35.072083 1 CACGGGG 235 0.0 34.43247 1 CGGGTCG 70 2.0295076E-4 33.998447 1 CGCGGAG 140 4.474714E-10 33.998447 1 CGCAGCC 140 4.474714E-10 33.998447 1 >>END_MODULE