FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_50220371-2.34100000035905.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_50220371-2.34100000035905.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477869
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2033485.846913727917872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1223743.5186489197839252No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT37350.10739334920320459TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT200.002057571771.3967361
CGCGAGG804.152753E-947.5978241
AACGGTG601.4721481E-647.5978241
TGTACCG405.3870457E-447.5047382
ATCGCGG459.530779E-442.3091771
GTCGAGC1155.4569682E-1141.298377
CGGACGG704.252177E-640.7981381
CGGCACC1051.027729E-940.718353
CGACGGG1650.040.3860361
CTCGCGG500.001600012438.078261
CGGTGCG651.3127516E-436.6137121
CGTACCG651.3279585E-436.5421072
TCGCGGC651.3279585E-436.5421072
CGGTCAG1054.5785782E-836.265011
ACTGGCG3050.035.8232462
CGACGCC958.5676584E-735.0720831
CACGGGG2350.034.432471
CGGGTCG702.0295076E-433.9984471
CGCGGAG1404.474714E-1033.9984471
CGCAGCC1404.474714E-1033.9984471
Produced by FastQC (version 0.11.2)