##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_50110001-2.341000000358f7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6109255 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.693818477048346 33.0 33.0 33.0 33.0 33.0 2 32.72143330078708 33.0 33.0 33.0 33.0 33.0 3 32.752313007068786 33.0 33.0 33.0 33.0 33.0 4 36.67519263805489 37.0 37.0 37.0 37.0 37.0 5 36.65094500065884 37.0 37.0 37.0 37.0 37.0 6 36.520528280453185 37.0 37.0 37.0 37.0 37.0 7 36.45633616537532 37.0 37.0 37.0 37.0 37.0 8 36.37689472775322 37.0 37.0 37.0 37.0 37.0 9 36.37013891219142 37.0 37.0 37.0 37.0 37.0 10-11 36.31860922485639 37.0 37.0 37.0 37.0 37.0 12-13 36.33057926048266 37.0 37.0 37.0 37.0 37.0 14-15 38.15484834075514 40.0 37.0 40.0 37.0 40.0 16-17 38.13978701167328 40.0 37.0 40.0 37.0 40.0 18-19 38.12447376971497 40.0 37.0 40.0 37.0 40.0 20-21 38.04177170538797 40.0 37.0 40.0 35.0 40.0 22-23 37.93158011901615 40.0 37.0 40.0 33.0 40.0 24-25 37.90174661231198 40.0 37.0 40.0 33.0 40.0 26-27 37.80648565168748 40.0 37.0 40.0 33.0 40.0 28-29 37.67993364166335 40.0 37.0 40.0 33.0 40.0 30-31 37.631274762634725 40.0 37.0 40.0 33.0 40.0 32-33 37.509130573204104 40.0 37.0 40.0 33.0 40.0 34-35 37.390562597239764 40.0 37.0 40.0 33.0 40.0 36-37 37.28451194130872 40.0 37.0 40.0 33.0 40.0 38-39 37.232511083593664 40.0 37.0 40.0 33.0 40.0 40-41 37.1100491794826 40.0 37.0 40.0 33.0 40.0 42-43 36.95882010163269 40.0 37.0 40.0 33.0 40.0 44-45 36.81682054522196 37.0 37.0 40.0 33.0 40.0 46-47 36.73241008928257 37.0 37.0 40.0 33.0 40.0 48-49 36.51728083047769 37.0 37.0 40.0 33.0 40.0 50-51 36.34515755194373 37.0 37.0 40.0 33.0 40.0 52-53 36.19388215093329 37.0 37.0 40.0 33.0 40.0 54-55 36.01742994849617 37.0 37.0 40.0 33.0 40.0 56-57 35.847095349596636 37.0 37.0 40.0 27.0 40.0 58-59 35.586413482495004 37.0 37.0 40.0 27.0 40.0 60-61 35.31311731135793 37.0 33.0 40.0 27.0 40.0 62-63 34.98619037836856 37.0 33.0 38.5 27.0 40.0 64-65 34.719382887111436 37.0 33.0 37.0 27.0 40.0 66-67 34.41530988639368 37.0 33.0 37.0 27.0 40.0 68-69 34.17035072197838 37.0 33.0 37.0 27.0 40.0 70-71 33.86825144800798 37.0 33.0 37.0 27.0 38.5 72-73 33.57704081757923 37.0 33.0 37.0 27.0 37.0 74-75 33.270392298242584 37.0 33.0 37.0 27.0 37.0 76-77 30.57909049466752 33.0 30.0 35.0 24.5 37.0 78-79 32.9737472081293 35.0 33.0 37.0 27.0 37.0 80-81 33.375241989407876 37.0 33.0 37.0 27.0 37.0 82-83 33.30095543564641 37.0 33.0 37.0 27.0 37.0 84-85 33.14345832675179 37.0 33.0 37.0 27.0 37.0 86-87 33.04895834271118 37.0 33.0 37.0 27.0 37.0 88-89 32.92368128356076 37.0 33.0 37.0 27.0 37.0 90-91 32.7843343419124 35.0 33.0 37.0 27.0 37.0 92-93 32.66129830887727 33.0 33.0 37.0 27.0 37.0 94-95 32.551173588268945 33.0 33.0 37.0 27.0 37.0 96-97 32.39157458642666 33.0 33.0 37.0 22.0 37.0 98-99 32.209150624748844 33.0 33.0 37.0 22.0 37.0 100-101 30.469136416797138 33.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10621273041364532 2306 2 0.10371062161708977 2306 3 0.0762777540193369 2306 4 0.1794621802876577 2306 5 0.15417311209911588 2306 6 0.16656957921499327 2306 7 0.1674386601672282 2306 8 0.22629443746937739 2306 9 0.17151196100257948 2306 10-11 0.2642479777395863 2306 12-13 0.20122565103193324 2306 14-15 0.41977891689085567 2306 16-17 0.42364091126990644 2306 18-19 0.3280782502383417 2306 20-21 0.43562139210477113 2306 22-23 0.4431460318894054 2306 24-25 0.4078932399761257 2306 26-27 0.40012228575361064 2306 28-29 0.38228516602428186 2306 30-31 0.4140679931638189 2306 32-33 0.5418607503831439 2306 34-35 0.46521932778831143 2306 36-37 0.5151430974756579 2306 38-39 0.548917296559253 2306 40-41 0.5259665995654714 2306 42-43 0.4784533749428874 2306 44-45 0.5302681924820476 2306 46-47 0.5454148690343885 2306 48-49 0.49092149851725964 2306 50-51 0.5827253737905593 2306 52-53 0.5163134192514249 2306 54-55 0.4963965164035713 2306 56-57 0.5175966586297847 2306 58-59 0.5776319933990237 2306 60-61 0.4766053004501316 2306 62-63 0.4543124846350608 2306 64-65 0.4679455555268035 2306 66-67 0.45863253752069255 2306 68-69 0.5009420147979355 2306 70-71 0.5128227324311041 2306 72-73 0.2640999866957756 2306 74-75 0.41959831094030164 2306 76-77 0.4309998542891087 2306 78-79 0.5453987309574515 2306 80-81 0.5052658421247855 2306 82-83 0.3925193251656651 2306 84-85 0.49752819850318986 2306 86-87 0.4413662682495243 2306 88-89 0.4562044102899918 2306 90-91 0.5362113733801266 2306 92-93 0.6392094998730187 2306 94-95 0.4195205512865883 2306 96-97 0.5505974352810412 2306 98-99 0.6592604450926345 2306 100-101 0.43332813170878737 2307 1 0.08564792239669572 2307 2 0.07808239824992569 2307 3 0.10807010294828245 2307 4 0.1513945397533476 2307 5 0.14873529206224845 2307 6 0.16808742669568488 2307 7 0.1426791348075156 2307 8 0.19905380099920933 2307 9 0.19440069940419136 2307 10-11 0.18572077399953457 2307 12-13 0.1485888281282115 2307 14-15 0.4010125867630592 2307 16-17 0.41079633283771244 2307 18-19 0.36046047202004416 2307 20-21 0.40982048261297876 2307 22-23 0.44726344480346825 2307 24-25 0.4494737737812571 2307 26-27 0.35763786385719243 2307 28-29 0.37773916608086466 2307 30-31 0.4436922570406878 2307 32-33 0.4419344888174024 2307 34-35 0.48366202290063853 2307 36-37 0.46721452478941217 2307 38-39 0.48028493604502387 2307 40-41 0.44105413904117796 2307 42-43 0.4547984670563352 2307 44-45 0.5039452431662497 2307 46-47 0.4957957627428229 2307 48-49 0.4878841961381468 2307 50-51 0.4628795216900059 2307 52-53 0.5413334452035556 2307 54-55 0.43072281844656146 2307 56-57 0.4609799930413274 2307 58-59 0.39374790434194296 2307 60-61 0.4097067007137696 2307 62-63 0.4713818020275582 2307 64-65 0.32152540343442126 2307 66-67 0.5762564437100153 2307 68-69 0.6525743054760369 2307 70-71 0.5299957900918031 2307 72-73 0.3765443230557963 2307 74-75 0.3754742240277693 2307 76-77 0.4374731975656836 2307 78-79 0.42505284829123013 2307 80-81 0.38668727817300663 2307 82-83 0.3670803566906429 2307 84-85 0.4752218399147097 2307 86-87 0.4006550506894442 2307 88-89 0.39824592182848306 2307 90-91 0.4246995371472053 2307 92-93 0.5967464689973525 2307 94-95 0.409881831531024 2307 96-97 0.5585172814200021 2307 98-99 0.6861444079303283 2307 100-101 0.5508188695204517 2201 1 0.10303768283289116 2201 2 0.10138042348351917 2201 3 0.0664850346036232 2201 4 0.14877396589012193 2201 5 0.1344988911949372 2201 6 0.17401203461289327 2201 7 0.22861793218451965 2201 8 0.1875580051305903 2201 9 0.21334800455539238 2201 10-11 0.2224003868382951 2201 12-13 0.21215564229758854 2201 14-15 0.4053750194146488 2201 16-17 0.3701806157865022 2201 18-19 0.3682985641823535 2201 20-21 0.42724270230100814 2201 22-23 0.41249132373255293 2201 24-25 0.32969289234414845 2201 26-27 0.4198780479751534 2201 28-29 0.4189765345516605 2201 30-31 0.3956556513577638 2201 32-33 0.4142345804569132 2201 34-35 0.37224514160190125 2201 36-37 0.3851783055304878 2201 38-39 0.39150777003460746 2201 40-41 0.45047419980307524 2201 42-43 0.4566670304207463 2201 44-45 0.482073677207552 2201 46-47 0.5313880587414204 2201 48-49 0.39659182398968795 2201 50-51 0.5140619055501148 2201 52-53 0.6012887476788435 2201 54-55 0.617128493358841 2201 56-57 0.5591299155613214 2201 58-59 0.48088215553514857 2201 60-61 0.5611316073922836 2201 62-63 0.5938613865200821 2201 64-65 0.466572793488119 2201 66-67 0.5414878863471628 2201 68-69 0.49631015562560776 2201 70-71 0.4347031215881145 2201 72-73 0.3731342630120835 2201 74-75 0.34186813915740544 2201 76-77 0.09702138384710324 2201 78-79 0.36204567248129393 2201 80-81 0.4011167094442314 2201 82-83 0.362883670512403 2201 84-85 0.4618220932875374 2201 86-87 0.5278989269905452 2201 88-89 0.37939996671885723 2201 90-91 0.5588047545156911 2201 92-93 0.45929783519591183 2201 94-95 0.4736629448588445 2201 96-97 0.40267689504685933 2201 98-99 0.3711119336972999 2201 100-101 0.45313595164889975 1101 1 0.09005178362262001 1101 2 0.04510487731981527 1101 3 0.06900202829896784 1101 4 0.1086513820844317 1101 5 0.13828286126974376 1101 6 0.17742664132003227 1101 7 0.2175871107655567 1101 8 0.19544140904972807 1101 9 0.1669292004800127 1101 10-11 0.17239396422262843 1101 12-13 0.2252000796617608 1101 14-15 0.36550526838126274 1101 16-17 0.2702857493874049 1101 18-19 0.3144831526752867 1101 20-21 0.32688908063840216 1101 22-23 0.3529565309962308 1101 24-25 0.35919005610866606 1101 26-27 0.35689208579806575 1101 28-29 0.37994638309966433 1101 30-31 0.35989175678437135 1101 32-33 0.33848020646674115 1101 34-35 0.2966415311683832 1101 36-37 0.41028504478529726 1101 38-39 0.3703123617430606 1101 40-41 0.446007018739472 1101 42-43 0.39531157306544173 1101 44-45 0.4104200593649381 1101 46-47 0.5242861780064558 1101 48-49 0.5151243654522872 1101 50-51 0.4941781590914758 1101 52-53 0.5045512475371652 1101 54-55 0.5620513317184006 1101 56-57 0.6114976988815712 1101 58-59 0.4934153652855997 1101 60-61 0.49122878198710396 1101 62-63 0.5906862426304045 1101 64-65 0.47828900874565505 1101 66-67 0.4196665878621886 1101 68-69 0.4788738455148547 1101 70-71 0.4486786283345836 1101 72-73 0.26907397354101903 1101 74-75 0.2525850080105343 1101 76-77 0.15973245387586488 1101 78-79 0.46623985040118754 1101 80-81 0.4747154773094948 1101 82-83 0.4127870164432963 1101 84-85 0.5336358567430253 1101 86-87 0.5990267718839632 1101 88-89 0.38767223273099916 1101 90-91 0.5570869986699591 1101 92-93 0.6742863731357289 1101 94-95 0.5188651460109739 1101 96-97 0.4745813307235238 1101 98-99 0.7010072366663493 1101 100-101 0.47657081688101144 2304 1 0.046530899113299995 2304 2 0.04566016621121349 2304 3 0.0771260694844571 2304 4 0.11980210066590047 2304 5 0.11665624128499275 2304 6 0.10645589751526785 2304 7 0.12476186676785517 2304 8 0.08356469730113503 2304 9 0.07581041100979036 2304 10-11 0.09733302361971852 2304 12-13 0.10959299125789101 2304 14-15 0.16707507713599767 2304 16-17 0.1962602696272171 2304 18-19 0.1977146046145677 2304 20-21 0.1435321592917873 2304 22-23 0.2647430082999733 2304 24-25 0.23160490396892186 2304 26-27 0.2540576418869449 2304 28-29 0.28730619618377773 2304 30-31 0.2466034112629174 2304 32-33 0.21316450355570993 2304 34-35 0.24742852376139268 2304 36-37 0.27679271294842067 2304 38-39 0.2343320533224329 2304 40-41 0.24460067903442706 2304 42-43 0.33970074352031077 2304 44-45 0.3357466445264876 2304 46-47 0.31732941995241504 2304 48-49 0.37664719817856707 2304 50-51 0.36245063417405987 2304 52-53 0.2945232242715008 2304 54-55 0.15222554184348525 2304 56-57 0.17421404978650656 2304 58-59 0.26541980524381614 2304 60-61 0.2541983658027931 2304 62-63 0.240741771519545 2304 64-65 0.23308684594248774 2304 66-67 0.24557404990959952 2304 68-69 0.2678003615005906 2304 70-71 0.29700629113305155 2304 72-73 0.2208916034927384 2304 74-75 0.1928550995861329 2304 76-77 0.021823457725240303 2304 78-79 0.34939753832882303 2304 80-81 0.28642989646790085 2304 82-83 0.365928163350155 2304 84-85 0.3887016325945467 2304 86-87 0.28739117551310045 2304 88-89 0.2609584253315447 2304 90-91 0.32492883074682766 2304 92-93 0.524833603279447 2304 94-95 0.21538741403667672 2304 96-97 0.47598572927036287 2304 98-99 0.47951067428105887 2304 100-101 0.464969396659086 2202 1 0.06289815630870521 2202 2 0.06518337965054855 2202 3 0.07954863926830313 2202 4 0.08920625635853696 2202 5 0.06666573233880513 2202 6 0.06728305587639483 2202 7 0.14491986677132473 2202 8 0.06832985593194252 2202 9 0.14202168794427195 2202 10-11 0.15454382281853185 2202 12-13 0.061894278829299765 2202 14-15 0.17253100435697633 2202 16-17 0.0840557039962917 2202 18-19 0.15142018112953082 2202 20-21 0.1991336931676173 2202 22-23 0.17080031169481913 2202 24-25 0.20660885375199456 2202 26-27 0.15061575317881193 2202 28-29 0.17629909843807212 2202 30-31 0.16473670616905878 2202 32-33 0.22234975662829015 2202 34-35 0.25191892434173724 2202 36-37 0.1853678041117348 2202 38-39 0.1910301149772451 2202 40-41 0.26829105828883826 2202 42-43 0.20040623626515242 2202 44-45 0.18863133169498525 2202 46-47 0.277749591509 2202 48-49 0.3464903512802593 2202 50-51 0.17726160088418652 2202 52-53 0.25970664612048466 2202 54-55 0.23960544962979213 2202 56-57 0.3079277342078086 2202 58-59 0.2421165372830103 2202 60-61 0.1342398913334364 2202 62-63 0.29221728315389583 2202 64-65 0.1976651769406672 2202 66-67 0.22498860886785366 2202 68-69 0.32133849542630344 2202 70-71 0.17804761454476647 2202 72-73 0.24391818770298102 2202 74-75 0.17809119389017525 2202 76-77 0.2660524882835382 2202 78-79 0.3552062151775459 2202 80-81 0.23578913820794156 2202 82-83 0.19268720574911669 2202 84-85 0.292188339766021 2202 86-87 0.27998061205701674 2202 88-89 0.2565258970305493 2202 90-91 0.2763778585793517 2202 92-93 0.38696275409633785 2202 94-95 0.20331297784289148 2202 96-97 0.25899906145203744 2202 98-99 0.43309922219612673 2202 100-101 0.4146811710058067 1102 1 0.07808502745824342 1102 2 0.048990049145274384 1102 3 0.06261551027213841 1102 4 0.09053004411237708 1102 5 0.10383899853371759 1102 6 0.11477162803413421 1102 7 0.08395779991925423 1102 8 0.10011103335681781 1102 9 0.0431435701722549 1102 10-11 0.13471537573543202 1102 12-13 0.11054406946638551 1102 14-15 0.20138637785398572 1102 16-17 0.15608731423642297 1102 18-19 0.08158369910763241 1102 20-21 0.12647254538980945 1102 22-23 0.2023632150928023 1102 24-25 0.1917243141982894 1102 26-27 0.1228373272897727 1102 28-29 0.18955599270927337 1102 30-31 0.1427645531634738 1102 32-33 0.18622058946569808 1102 34-35 0.16520403360328118 1102 36-37 0.14430682202726075 1102 38-39 0.18541970295667198 1102 40-41 0.2150124734566745 1102 42-43 0.22322711967656517 1102 44-45 0.16050371330499758 1102 46-47 0.17712403452301118 1102 48-49 0.18864697282952392 1102 50-51 0.09307211257935677 1102 52-53 0.1390702520579481 1102 54-55 0.10819580567520859 1102 56-57 0.22834920473254527 1102 58-59 0.19783165290786542 1102 60-61 0.09888886787715734 1102 62-63 0.15463243513533342 1102 64-65 0.07011883395028207 1102 66-67 0.012233421623257357 1102 68-69 0.13732011833824487 1102 70-71 0.1231023500836983 1102 72-73 0.02231390264730493 1102 74-75 0.06220022123334701 1102 76-77 0.04124325203851953 1102 78-79 0.2257371475917438 1102 80-81 0.22924226284747817 1102 82-83 0.22660467273474438 1102 84-85 0.18363364198161491 1102 86-87 0.17238180454234708 1102 88-89 0.15146205774350818 1102 90-91 0.11929772547993878 1102 92-93 0.07347891915606652 1102 94-95 -0.1662208641150258 1102 96-97 0.0429435440119903 1102 98-99 0.24026577723998344 1102 100-101 0.19633486740746164 2305 1 0.031682264239385916 2305 2 0.024722933690753734 2305 3 0.029210646449605804 2305 4 0.018657722513395925 2305 5 0.02515310233196999 2305 6 0.0021461802895075266 2305 7 0.02344140614241752 2305 8 0.03620410513762096 2305 9 0.035379151080476845 2305 10-11 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1213 88-89 -0.3748124789401075 1213 90-91 -0.2877474709150931 1213 92-93 -0.44500766166945027 1213 94-95 -0.2978322239824749 1213 96-97 -0.333487780370362 1213 98-99 -0.19608816312383226 1213 100-101 -0.13708737482712507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 53.0 9 205.0 10 336.0 11 262.0 12 266.0 13 631.0 14 1579.0 15 3283.0 16 5641.0 17 8598.0 18 11510.0 19 13933.0 20 17025.0 21 20434.0 22 25151.0 23 30572.0 24 36647.0 25 43614.0 26 53023.0 27 65053.0 28 81877.0 29 103887.0 30 121220.0 31 148455.0 32 195279.0 33 270306.0 34 406899.0 35 690828.0 36 1314880.0 37 1969574.0 38 468047.0 39 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.069195518905715 28.940206539933783 16.473044703525442 16.517553237635067 2 33.37004266477664 23.47025946698902 28.452519988116393 14.707177880117953 3 39.115898747064904 20.61601619182699 21.89725261099758 18.37083245011053 4 33.216469097724385 20.648097953110334 27.32735318961101 18.80807975955428 5 34.9598928183551 18.609372173857533 27.01435772446886 19.416377283318507 6 32.10083717245392 19.57335223361932 28.70927797251874 19.616532621408012 7 35.841375094017195 20.063772096597702 22.2946005691365 21.800252240248607 8 31.020803682282043 26.0170020730842 22.215622035747405 20.746572208886356 9 29.293293535791186 22.655495637356765 26.924330380709264 21.126880446142778 10-11 22.537698950199328 30.153201986166888 31.461127420610207 15.847971643023575 12-13 22.41188982944729 29.35405380852493 31.599859557343734 16.63419680468404 14-15 22.328278980006562 29.062168791448386 31.87307617704614 16.736476051498915 16-17 22.05678106413957 28.93685072893503 31.88933838905071 17.117029817874684 18-19 20.992649676597228 29.298285961217857 31.944426939127602 17.764637423057312 20-21 21.57902231941538 29.065753516590814 31.23129579629595 18.12392836769786 22-23 20.958177388241282 28.536711923139563 31.55065552182713 18.954455166792023 24-25 20.491655692879082 27.820814485563293 32.204245853217785 19.483283968339837 26-27 20.49532226106129 28.260352530709554 31.663697128373265 19.58062807985589 28-29 20.646551829969447 28.782396544259488 30.9923223044381 19.57872932133296 30-31 19.735786114673555 30.448933626113167 30.54072059522806 19.274559663985215 32-33 19.382559739280815 31.134811036697602 31.076702478452773 18.405926745568813 34-35 19.968523166900056 30.318344871837894 32.18635496472156 17.526776996540494 36-37 19.65676665976457 29.96916154261035 33.153076766316026 17.220995031309055 38-39 20.123893993621152 29.445136927497707 31.80905036702511 18.62191871185603 40-41 20.3165443249627 29.144920288971406 31.394400790276393 19.144134595789502 42-43 20.3632030419421 28.81666422501598 31.408109499439785 19.41202323360213 44-45 19.989573196731843 28.36756691282325 31.810752702252564 19.832107188192342 46-47 20.682622029669098 28.65987521876655 31.02694077769259 19.63056197387176 48-49 20.451211905510796 29.779925197696887 30.439710091763246 19.329152805029075 50-51 21.388106446118062 29.623789366633435 28.93971199904145 20.04839218820705 52-53 21.040328958627516 29.582901497791042 28.82589921439283 20.550870329188612 54-55 21.689546790207327 29.380746973553894 29.127532269814115 19.80217396642466 56-57 21.18570072471758 30.095297779059077 29.02480109495489 19.694200401268454 58-59 20.781481994742133 31.06137097000615 28.76805811568179 19.38908891956993 60-61 20.944380427540164 31.039929442540583 28.5182336048725 19.49745652504676 62-63 20.366515933021784 30.771966275669303 29.275507006286748 19.586010785022165 64-65 20.769997526455548 29.69783960201963 29.885368976383138 19.646793895141688 66-67 21.053583278891345 29.612658165885158 29.352539916327007 19.98121863889649 68-69 21.224014615080332 30.30679159822806 29.47518887744971 18.994004909241895 70-71 20.50928413577985 30.905962495411714 29.616535575134424 18.96821779367401 72-73 20.304741841134025 30.35274872501028 29.662069093666343 19.68044034018935 74-75 20.596902445940646 29.37600975520947 29.34238945001863 20.684698348831255 76-77 20.569424497679172 28.72128740163442 28.9508613114428 21.758426789243604 78-79 19.880813452402567 29.29735937848625 29.343603935747403 21.478223233363785 80-81 19.433632794771995 29.887612952808734 28.973047652058902 21.70570660036037 82-83 18.620062112706094 30.117836704769203 29.0232774511038 22.2388237314209 84-85 18.34434739679037 30.508291464668964 30.018725394733924 21.128635743806743 86-87 18.790197604490444 30.180728986412177 30.648423397686607 20.380650011410776 88-89 19.562924360976258 30.011007969154115 30.11617631217317 20.30989135769646 90-91 19.528461572338298 30.128805737549385 29.519034765728797 20.823697924383524 92-93 18.91674811602318 30.583791332478725 29.739427546090514 20.760033005407582 94-95 19.06456631835946 30.05153842117632 29.818424087182372 21.065471173281846 96-97 19.365040800083236 30.689154388877103 29.389337566241863 20.556467244797798 98-99 20.1483114086039 30.451968215405124 28.768174209785464 20.63154616620551 100-101 19.87628177370386 30.93263438620158 28.025444338667388 21.165639501427172 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13684.0 1 7660.0 2 1841.5 3 2047.5 4 1654.0 5 1137.0 6 1034.5 7 1023.0 8 1002.5 9 983.0 10 976.0 11 929.0 12 855.5 13 847.0 14 851.0 15 888.0 16 941.0 17 1003.5 18 1074.5 19 1282.0 20 1651.0 21 2145.5 22 2879.0 23 3999.5 24 5762.0 25 8004.0 26 10687.5 27 14402.5 28 19419.5 29 25429.0 30 33114.5 31 42609.0 32 59911.0 33 95523.0 34 144965.0 35 189736.0 36 247535.5 37 334520.5 38 415982.5 39 459987.0 40 463470.5 41 451243.5 42 451212.5 43 445551.0 44 395827.5 45 327175.0 46 281665.5 47 246112.0 48 208649.0 49 167428.0 50 122491.5 51 92133.5 52 75358.0 53 57825.0 54 41419.0 55 30447.0 56 22855.5 57 17474.0 58 13336.5 59 9954.0 60 7470.5 61 5856.0 62 4776.5 63 3692.0 64 2678.0 65 2005.0 66 1539.5 67 1272.0 68 1073.5 69 899.0 70 776.5 71 617.0 72 455.5 73 329.5 74 249.0 75 185.5 76 149.0 77 134.0 78 98.5 79 75.5 80 46.5 81 26.0 82 21.0 83 15.0 84 14.0 85 12.5 86 8.5 87 5.5 88 3.0 89 1.5 90 0.5 91 1.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18902468467922848 2 0.0 3 0.0 4 0.02615703551415025 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.9105823868867806E-5 48-49 0.0035765408384492055 50-51 7.36587358033017E-5 52-53 0.002594424361071849 54-55 0.009903007813555008 56-57 0.014330716265731255 58-59 0.014510770953250436 60-61 0.0031345884236293952 62-63 0.002201577770120907 64-65 0.010017588069249033 66-67 0.01753896342516395 68-69 0.026042455258456228 70-71 0.021426507814782652 72-73 0.017489857601295084 74-75 0.02191756605347133 76-77 0.01608215731705421 78-79 0.007005764205291807 80-81 0.003805701349837255 82-83 6.220071023389923E-4 84-85 0.0028072162645036095 86-87 0.016254027700595242 88-89 0.015599283382343674 90-91 0.018692950286082345 92-93 0.019069428269076996 94-95 0.017899072800202316 96-97 0.020305258169776838 98-99 0.028939698866719425 100-101 0.016695980115415055 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6109255.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.4278696839169 #Duplication Level Percentage of deduplicated Percentage of total 1 80.68135971403693 29.390500575841607 2 9.029537139816465 6.578536044706438 3 3.296822971871025 3.6028871277078407 4 1.7246281735039344 2.5129812143045185 5 1.056561797046619 1.9244147727909653 6 0.6758639549851589 1.4772170445753627 7 0.48956236575839285 1.248359984339776 8 0.36476009987325325 1.0629946707260307 9 0.31443813159208023 1.0308880153161553 >10 1.8994523845737452 13.59227466270187 >50 0.22990523107901598 5.814465143917236 >100 0.1966252714137049 14.702868973885494 >500 0.025399222857338942 6.504334840993755 >1k 0.01481499023459609 9.103508677961932 >5k 1.3427797003928157E-4 0.378368464886755 >10k+ 1.3427338810159022E-4 1.0753997853443522 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 28017 0.45859928911135645 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 19893 0.3256207180744624 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18048 0.29542063639510874 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9173 0.15014924078304148 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 7836 0.12826441194548271 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 6197 0.1014362635051246 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.05638494382703E-4 0.0 0.0 2 7.365873580330171E-4 0.0 0.0 3 8.347990057707527E-4 0.0 0.0 4 9.493792614647776E-4 0.0 0.0 5 0.0010803281251150919 0.0 0.0 6 0.0012931200285468522 0.0 0.0 7 0.0014404375001534557 0.0 0.0 8 0.0019151271308858445 0.0 0.0 9 0.00224249929001163 0.0 0.0 10-11 0.0031345884236293952 0.0 0.0 12-13 0.005000609730646371 0.0 0.0 14-15 0.008552597657161144 0.0 0.0 16-17 0.01627858061252968 0.0 0.0 18-19 0.026500776281232326 0.0 0.0 20-21 0.03391575568543137 0.0 0.0 22-23 0.04208369105561971 0.0 0.0 24-25 0.050799974792343744 0.0 0.0 26-27 0.06146412287586621 0.0 0.0 28-29 0.07266843502194621 0.0 0.0 30-31 0.08604158772223454 0.0 0.0 32-33 0.0993983718145666 0.0 0.0 34-35 0.11387640555190445 0.0 0.0 36-37 0.12950024184618256 0.0 0.0 38-39 0.14671183311222072 0.0 0.0 40-41 0.16566668112560368 0.0 0.0 42-43 0.1850471129458502 0.0 0.0 44-45 0.2048858657888728 0.0 0.0 46-47 0.22518293965467148 0.0 0.0 48-49 0.24703503127631765 0.0 0.0 50-51 0.2705485366055272 0.0 0.0 52-53 0.29547792652295574 0.0 0.0 54-55 0.3220769144519258 1.636860795628927E-5 1.636860795628927E-5 56-57 0.35023092013674334 1.636860795628927E-5 1.636860795628927E-5 58-59 0.3797598888898892 1.636860795628927E-5 1.636860795628927E-5 60-61 0.4116131999728281 1.636860795628927E-5 1.636860795628927E-5 62-63 0.4463801232719865 1.636860795628927E-5 1.636860795628927E-5 64-65 0.4847317717135723 1.636860795628927E-5 1.636860795628927E-5 66-67 0.5284359549568647 1.636860795628927E-5 1.636860795628927E-5 68-69 0.5774435671779947 1.636860795628927E-5 1.636860795628927E-5 70-71 0.6320410590161976 1.636860795628927E-5 1.636860795628927E-5 72-73 0.6940126087387087 1.636860795628927E-5 1.636860795628927E-5 74-75 0.7675895015022289 1.636860795628927E-5 1.636860795628927E-5 76-77 0.8562500664974698 1.636860795628927E-5 1.636860795628927E-5 78-79 0.96546960308581 1.636860795628927E-5 1.636860795628927E-5 80-81 1.1012390217792514 1.636860795628927E-5 1.636860795628927E-5 82-83 1.2684116148368336 1.636860795628927E-5 1.636860795628927E-5 84-85 1.4760719596742975 1.636860795628927E-5 1.636860795628927E-5 86-87 1.7232461241182435 1.636860795628927E-5 1.636860795628927E-5 88-89 2.0120456585950333 1.636860795628927E-5 1.636860795628927E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGACGG 135 0.0 56.416996 1 CGCGGAG 395 0.0 50.614616 1 GTATGCG 120 0.0 47.601845 1 CGAGTGG 295 0.0 46.795033 1 GGACGGG 800 0.0 46.411797 1 CGACGGG 295 0.0 45.181408 1 GTTACGG 245 0.0 44.687443 1 CGGGTGG 805 0.0 43.758217 1 GTCGCAC 100 6.2391337E-10 42.84166 1 CGAGGGG 1145 0.0 41.157925 1 ATTACGG 225 0.0 40.197117 1 CGCGGGG 500 0.0 38.081474 1 CACGGGG 660 0.0 37.504486 1 CGAGCGG 200 0.0 35.70138 1 AGTCGCC 80 1.0626554E-5 35.70138 1 TCAGCCG 415 0.0 35.488136 2 GGTCCGG 95 8.563402E-7 35.075043 1 CGGATGG 150 2.3646862E-11 34.90802 1 CGTTTGA 685 0.0 34.745872 1 GTCCCGT 235 0.0 34.357025 9 >>END_MODULE