FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_50110001-2.341000000358f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_50110001-2.341000000358f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6109255
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT280170.45859928911135645TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC198930.3256207180744624No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180480.29542063639510874No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91730.15014924078304148No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG78360.12826441194548271No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA61970.1014362635051246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACGG1350.056.4169961
CGCGGAG3950.050.6146161
GTATGCG1200.047.6018451
CGAGTGG2950.046.7950331
GGACGGG8000.046.4117971
CGACGGG2950.045.1814081
GTTACGG2450.044.6874431
CGGGTGG8050.043.7582171
GTCGCAC1006.2391337E-1042.841661
CGAGGGG11450.041.1579251
ATTACGG2250.040.1971171
CGCGGGG5000.038.0814741
CACGGGG6600.037.5044861
CGAGCGG2000.035.701381
AGTCGCC801.0626554E-535.701381
TCAGCCG4150.035.4881362
GGTCCGG958.563402E-735.0750431
CGGATGG1502.3646862E-1134.908021
CGTTTGA6850.034.7458721
GTCCCGT2350.034.3570259
Produced by FastQC (version 0.11.2)