##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_44065-1.341000000357de.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3955270 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.687056003762066 33.0 33.0 33.0 33.0 33.0 2 32.72181014191193 33.0 33.0 33.0 33.0 33.0 3 32.74722104938475 33.0 33.0 33.0 33.0 33.0 4 36.68039830403487 37.0 37.0 37.0 37.0 37.0 5 36.67381089028056 37.0 37.0 37.0 37.0 37.0 6 36.542163240436174 37.0 37.0 37.0 37.0 37.0 7 36.49420292419987 37.0 37.0 37.0 37.0 37.0 8 36.43167571366809 37.0 37.0 37.0 37.0 37.0 9 36.44039673650598 37.0 37.0 37.0 37.0 37.0 10-11 36.404542547032186 37.0 37.0 37.0 37.0 37.0 12-13 36.39518667499311 37.0 37.0 37.0 37.0 37.0 14-15 38.27565261031485 40.0 37.0 40.0 37.0 40.0 16-17 38.24946021384128 40.0 37.0 40.0 37.0 40.0 18-19 38.241534711916 40.0 37.0 40.0 37.0 40.0 20-21 38.1686802670867 40.0 37.0 40.0 37.0 40.0 22-23 38.0774667721799 40.0 37.0 40.0 37.0 40.0 24-25 38.0472587206436 40.0 37.0 40.0 35.0 40.0 26-27 37.96286182738473 40.0 37.0 40.0 35.0 40.0 28-29 37.844620215560504 40.0 37.0 40.0 33.0 40.0 30-31 37.80687411984517 40.0 37.0 40.0 33.0 40.0 32-33 37.70427278036645 40.0 37.0 40.0 33.0 40.0 34-35 37.61605402412478 40.0 37.0 40.0 33.0 40.0 36-37 37.53656956415112 40.0 37.0 40.0 33.0 40.0 38-39 37.463739517150536 40.0 37.0 40.0 33.0 40.0 40-41 37.376297951846524 40.0 37.0 40.0 33.0 40.0 42-43 37.25116578640649 40.0 37.0 40.0 33.0 40.0 44-45 37.121914559562306 40.0 37.0 40.0 33.0 40.0 46-47 37.04939738627199 40.0 37.0 40.0 33.0 40.0 48-49 36.85611399474625 37.0 37.0 40.0 33.0 40.0 50-51 36.69626637372417 37.0 37.0 40.0 33.0 40.0 52-53 36.553288523918724 37.0 37.0 40.0 33.0 40.0 54-55 36.386959044515294 37.0 37.0 40.0 33.0 40.0 56-57 36.21469027398888 37.0 37.0 40.0 33.0 40.0 58-59 35.961729667001244 37.0 37.0 40.0 33.0 40.0 60-61 35.699088810624815 37.0 37.0 40.0 27.0 40.0 62-63 35.4127440098906 37.0 33.0 40.0 27.0 40.0 64-65 35.162422666467776 37.0 33.0 37.0 27.0 40.0 66-67 34.86486346064871 37.0 33.0 37.0 27.0 40.0 68-69 34.58966846763938 37.0 33.0 37.0 27.0 40.0 70-71 34.255933476096445 37.0 33.0 37.0 27.0 40.0 72-73 33.95293469219548 37.0 33.0 37.0 27.0 37.0 74-75 33.60876071671466 37.0 33.0 37.0 27.0 37.0 76-77 30.976074578979436 33.0 30.0 35.0 24.5 37.0 78-79 33.329619090479284 35.0 33.0 37.0 27.0 37.0 80-81 33.718181312527335 37.0 33.0 37.0 27.0 37.0 82-83 33.63772726008591 37.0 33.0 37.0 27.0 37.0 84-85 33.46478369365429 37.0 33.0 37.0 27.0 37.0 86-87 33.36047362126985 37.0 33.0 37.0 27.0 37.0 88-89 33.22571935670637 37.0 33.0 37.0 27.0 37.0 90-91 33.086775365524986 35.0 33.0 37.0 27.0 37.0 92-93 32.95982031568009 33.0 33.0 37.0 27.0 37.0 94-95 32.83806149769801 33.0 33.0 37.0 27.0 37.0 96-97 32.671449610266805 33.0 33.0 37.0 27.0 37.0 98-99 32.50318145158232 33.0 33.0 37.0 27.0 37.0 100-101 30.76913649384239 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11280601103000976 2306 2 0.10198568912806394 2306 3 0.09884775913356236 2306 4 0.14760314117915385 2306 5 0.1675187189073526 2306 6 0.25045037295249273 2306 7 0.17964152040735115 2306 8 0.2768879335912615 2306 9 0.233708080725755 2306 10-11 0.2413506675688808 2306 12-13 0.26785296665396885 2306 14-15 0.5277113221750156 2306 16-17 0.4931456723493355 2306 18-19 0.43311539286359846 2306 20-21 0.5102420288291682 2306 22-23 0.5129048410882149 2306 24-25 0.4628325686324004 2306 26-27 0.5090049530502156 2306 28-29 0.5186046431058955 2306 30-31 0.4713803902500615 2306 32-33 0.5692630880700378 2306 34-35 0.597955631272086 2306 36-37 0.5976185114635939 2306 38-39 0.6670155353833565 2306 40-41 0.5892115709567989 2306 42-43 0.6359299059166119 2306 44-45 0.6255089642825951 2306 46-47 0.548182026218889 2306 48-49 0.5503997646623446 2306 50-51 0.5604575975424027 2306 52-53 0.5699683003796849 2306 54-55 0.5967421618863327 2306 56-57 0.7488312652617566 2306 58-59 0.6011307932041987 2306 60-61 0.5437409133616526 2306 62-63 0.4783526314127613 2306 64-65 0.5566357779084896 2306 66-67 0.6093566598049804 2306 68-69 0.6759376479654335 2306 70-71 0.6751250714382451 2306 72-73 0.5205251298036089 2306 74-75 0.5537317217624675 2306 76-77 0.5051332505006734 2306 78-79 0.5482635633165813 2306 80-81 0.5370796265329005 2306 82-83 0.5239331622799455 2306 84-85 0.6120773024654369 2306 86-87 0.5687928446251718 2306 88-89 0.5399878236528082 2306 90-91 0.5382688526509583 2306 92-93 0.5785277717676536 2306 94-95 0.6111492838909243 2306 96-97 0.6081691382579137 2306 98-99 0.6810495957145122 2306 100-101 0.49163081816605114 2307 1 0.10871812828243321 2307 2 0.10403590945716701 2307 3 0.12190046276121791 2307 4 0.150672701821982 2307 5 0.16177613344851238 2307 6 0.2042974492785632 2307 7 0.1960954379663704 2307 8 0.23087970872546748 2307 9 0.19637092345206497 2307 10-11 0.20858792722913932 2307 12-13 0.2040965109262558 2307 14-15 0.4532084061376622 2307 16-17 0.5163320441879407 2307 18-19 0.4232524551044534 2307 20-21 0.4620362827365483 2307 22-23 0.4864144942749249 2307 24-25 0.5200607209790888 2307 26-27 0.5201256617236609 2307 28-29 0.4620281445002732 2307 30-31 0.5062459018152197 2307 32-33 0.5906935585391508 2307 34-35 0.5707223313133838 2307 36-37 0.5091238768591921 2307 38-39 0.5001075531906949 2307 40-41 0.41338430445190966 2307 42-43 0.5225846934786276 2307 44-45 0.5652095542781268 2307 46-47 0.5023111464297756 2307 48-49 0.48838438970689424 2307 50-51 0.5055121565730616 2307 52-53 0.45485779642281443 2307 54-55 0.3016668427060978 2307 56-57 0.5156937108865733 2307 58-59 0.5305818088676446 2307 60-61 0.5753837369682628 2307 62-63 0.4375467190126727 2307 64-65 0.4539416535399212 2307 66-67 0.5848868355591605 2307 68-69 0.6550721703405742 2307 70-71 0.5852745784554045 2307 72-73 0.49694154333221263 2307 74-75 0.4045247368489342 2307 76-77 0.41956986982062716 2307 78-79 0.4751725637182105 2307 80-81 0.42687298667907214 2307 82-83 0.29128791194196424 2307 84-85 0.4994887324268049 2307 86-87 0.4927401579676598 2307 88-89 0.39227800189594575 2307 90-91 0.4470547515880625 2307 92-93 0.5226781612325837 2307 94-95 0.3768868914392485 2307 96-97 0.5649095685077796 2307 98-99 0.4569169117722396 2307 100-101 0.4638204199897764 2201 1 0.09818731856340435 2201 2 0.09911773207522856 2201 3 0.08433810090456717 2201 4 0.12668023821876062 2201 5 0.12388006161265963 2201 6 0.13365709041246987 2201 7 0.13391943387458127 2201 8 0.15020884415358182 2201 9 0.14683372815030538 2201 10-11 0.17634232628761026 2201 12-13 0.14983982981134858 2201 14-15 0.33329906210166627 2201 16-17 0.336559701824676 2201 18-19 0.34280705614253293 2201 20-21 0.3803543719155016 2201 22-23 0.40754140479587875 2201 24-25 0.3391932002918381 2201 26-27 0.3783825307265758 2201 28-29 0.4217647881419637 2201 30-31 0.39243582005783395 2201 32-33 0.35143775162539015 2201 34-35 0.4887399040612692 2201 36-37 0.24535844459934708 2201 38-39 0.3279265126564681 2201 40-41 0.3881622704076477 2201 42-43 0.33853053997933813 2201 44-45 0.40628860554807034 2201 46-47 0.417480232589476 2201 48-49 0.4747848990921568 2201 50-51 0.5320795731390433 2201 52-53 0.4937889746110997 2201 54-55 0.47852457234047563 2201 56-57 0.45635340656018997 2201 58-59 0.4404212429072132 2201 60-61 0.23428688852864354 2201 62-63 0.4715338852178803 2201 64-65 0.4098392232607253 2201 66-67 0.5324663421965639 2201 68-69 0.5165468824413395 2201 70-71 0.44595671630779066 2201 72-73 0.35721512914875575 2201 74-75 0.397712804916587 2201 76-77 0.20469529811395049 2201 78-79 0.39959121995360647 2201 80-81 0.41406836672171465 2201 82-83 0.48093142054175075 2201 84-85 0.49428120751207416 2201 86-87 0.5212867137456101 2201 88-89 0.36112742270506004 2201 90-91 0.517839575456108 2201 92-93 0.45837166369992133 2201 94-95 0.3001961733678158 2201 96-97 0.38193363122061186 2201 98-99 0.27223161113837335 2201 100-101 0.35678610172314507 1101 1 0.07176981576749597 1101 2 0.02424572869563235 1101 3 0.06199178696213181 1101 4 0.05152833159417014 1101 5 0.10132555040764402 1101 6 0.1342636115236857 1101 7 0.1514688482837343 1101 8 0.12119204755586566 1101 9 0.1349094846556298 1101 10-11 0.1828870831721261 1101 12-13 0.1893130599784243 1101 14-15 0.36273580968748576 1101 16-17 0.2945875758432237 1101 18-19 0.3315201884780379 1101 20-21 0.3355940270539435 1101 22-23 0.3710186931755146 1101 24-25 0.3500392107334207 1101 26-27 0.35311357321717907 1101 28-29 0.3168276966846335 1101 30-31 0.35441727935147327 1101 32-33 0.4058777793273478 1101 34-35 0.4254911905156149 1101 36-37 0.4352247360111079 1101 38-39 0.505095414367851 1101 40-41 0.5101658182197255 1101 42-43 0.4274963253641033 1101 44-45 0.4334332608330911 1101 46-47 0.5701451527444803 1101 48-49 0.5787737125744172 1101 50-51 0.5960541896057592 1101 52-53 0.6202011427126095 1101 54-55 0.535088481258498 1101 56-57 0.5536382874114665 1101 58-59 0.5329270546001936 1101 60-61 0.5156393440980906 1101 62-63 0.48665322617646467 1101 64-65 0.5070108259615225 1101 66-67 0.6090575589826841 1101 68-69 0.6280490122339799 1101 70-71 0.5671081067646426 1101 72-73 0.4755263955907907 1101 74-75 0.4970857139927247 1101 76-77 0.21471635333572436 1101 78-79 0.4809174345938345 1101 80-81 0.4034768836814777 1101 82-83 0.36214618332326154 1101 84-85 0.5005370738736588 1101 86-87 0.5541970682913302 1101 88-89 0.44418914319687275 1101 90-91 0.5872945090631845 1101 92-93 0.713360828503049 1101 94-95 0.49339669684071197 1101 96-97 0.6429809422703059 1101 98-99 0.6810008394676217 1101 100-101 0.5125455291133889 2304 1 0.08428306943312691 2304 2 0.06133626608273346 2304 3 0.04219501357123079 2304 4 0.0577022421036304 2304 5 0.09039892583887621 2304 6 0.10023873386089832 2304 7 0.09961714587420545 2304 8 0.11729653463620338 2304 9 0.07718738908577194 2304 10-11 0.07173158769239052 2304 12-13 0.08398940154079781 2304 14-15 0.09403777358371457 2304 16-17 0.12415541107410633 2304 18-19 0.19050522108261703 2304 20-21 0.2306561539618599 2304 22-23 0.20295145309867735 2304 24-25 0.23413951940653277 2304 26-27 0.2317662610493585 2304 28-29 0.22036746216480907 2304 30-31 0.18178674335418066 2304 32-33 0.05606192962448375 2304 34-35 0.07794880680602745 2304 36-37 0.27725493979812654 2304 38-39 0.1910925943913 2304 40-41 0.26216995179426306 2304 42-43 0.2143314284680926 2304 44-45 0.25964424798179664 2304 46-47 0.26707702722190163 2304 48-49 0.3877315093164242 2304 50-51 0.2626372432951527 2304 52-53 0.2401886792232304 2304 54-55 0.2802125893680554 2304 56-57 0.26156403550103846 2304 58-59 0.19672039380660777 2304 60-61 0.2324231361819784 2304 62-63 0.32885917413018717 2304 64-65 0.2321142213657197 2304 66-67 0.23779643767213798 2304 68-69 0.2868493341327323 2304 70-71 0.22158879844347013 2304 72-73 0.25439136623658243 2304 74-75 0.17814345177973934 2304 76-77 0.04248908743524993 2304 78-79 0.3392799652201717 2304 80-81 0.3053970680335283 2304 82-83 0.2865634143214919 2304 84-85 0.30764462526873615 2304 86-87 0.2352209573931674 2304 88-89 0.160954105548619 2304 90-91 0.1881382603941546 2304 92-93 0.3679939513468753 2304 94-95 0.16324334660544793 2304 96-97 0.28534896041858104 2304 98-99 0.42670597931200405 2304 100-101 0.2726885902450107 2202 1 0.05628800737000006 2202 2 0.05253712549737344 2202 3 0.05975262454643371 2202 4 0.0839567019602967 2202 5 0.09643454717881639 2202 6 0.03839867453992696 2202 7 0.09514588922678513 2202 8 0.1049689464814989 2202 9 0.11429339656662307 2202 10-11 0.1411174061184468 2202 12-13 0.05631661996645221 2202 14-15 0.07768712255247578 2202 16-17 0.025739700054323578 2202 18-19 0.06443829601641937 2202 20-21 0.04439528546831184 2202 22-23 -0.0016362681995687467 2202 24-25 0.04176271620713834 2202 26-27 0.0267607459243564 2202 28-29 0.06885064873625169 2202 30-31 0.009674789397770667 2202 32-33 0.04217182542250697 2202 34-35 0.06737804785498014 2202 36-37 0.11556646938775827 2202 38-39 0.0694624179278378 2202 40-41 -0.04829440735232282 2202 42-43 0.04286732196969467 2202 44-45 -0.06858045690253078 2202 46-47 0.06755890741736437 2202 48-49 0.13010759359821833 2202 50-51 -0.03360261226403338 2202 52-53 0.01060891736982228 2202 54-55 0.07841016024820391 2202 56-57 0.04896572351955086 2202 58-59 0.03628325082622297 2202 60-61 -0.17485313152650406 2202 62-63 -0.0681540313590574 2202 64-65 -0.08193856361834406 2202 66-67 -0.07822392207422979 2202 68-69 0.0016210367144751103 2202 70-71 -0.1440023674970874 2202 72-73 -0.13773854011926545 2202 74-75 -0.04472408457181842 2202 76-77 0.043603204306602805 2202 78-79 0.14088253524788286 2202 80-81 0.10819530072866712 2202 82-83 0.07006795274558186 2202 84-85 0.03305651370094154 2202 86-87 -0.014934842320499797 2202 88-89 0.042021307911603856 2202 90-91 0.03366032808332875 2202 92-93 0.032237044089299616 2202 94-95 -0.10977608676424211 2202 96-97 0.07775164465149231 2202 98-99 0.2110900620655798 2202 100-101 0.2716572515999047 1102 1 0.035842291997504105 1102 2 0.04578030142883449 1102 3 0.054941588302341415 1102 4 0.06518372110572557 1102 5 0.07848412242922365 1102 6 0.10128583967604499 1102 7 0.059848881492534645 1102 8 0.10686062175183508 1102 9 0.09636108480586358 1102 10-11 0.07892883632004555 1102 12-13 0.04794932477907565 1102 14-15 0.1106193663895283 1102 16-17 0.08096801427846145 1102 18-19 0.007018196938375354 1102 20-21 -0.0030891600025313437 1102 22-23 0.11937740257874907 1102 24-25 0.05942514443442093 1102 26-27 0.051887763258484654 1102 28-29 0.13725197370137465 1102 30-31 0.015751017911242116 1102 32-33 0.10252453059739253 1102 34-35 0.07704562922759095 1102 36-37 0.048230527784028254 1102 38-39 0.04450837963698007 1102 40-41 -0.003628682561071628 1102 42-43 0.05199683436803326 1102 44-45 0.14396890099202153 1102 46-47 0.19368757303347905 1102 48-49 0.1112233310685582 1102 50-51 0.10562185747822639 1102 52-53 0.10253833558061842 1102 54-55 0.08933557548846949 1102 56-57 0.13678383288578289 1102 58-59 0.06689182460948473 1102 60-61 -0.0715466054166285 1102 62-63 0.04910368477418103 1102 64-65 -0.010820743154290824 1102 66-67 0.07688801619670471 1102 68-69 -0.07241897487784144 1102 70-71 -0.26102461477561434 1102 72-73 -0.12804910995389207 1102 74-75 -0.13126432257649157 1102 76-77 -0.21531846154497813 1102 78-79 0.02345817961746377 1102 80-81 0.0807602357935977 1102 82-83 -0.017122654764065715 1102 84-85 -0.10006557690023499 1102 86-87 0.060354286263617496 1102 88-89 -0.06489830664400387 1102 90-91 -0.06872594498808837 1102 92-93 0.006220565797718791 1102 94-95 -0.23811114083151352 1102 96-97 -0.09332734609277082 1102 98-99 0.03524237418860565 1102 100-101 -0.017151638255008095 2305 1 0.07625044884756704 2305 2 0.027034006230259422 2305 3 0.04428635487739996 2305 4 0.005143484716946034 2305 5 0.05772937579273929 2305 6 -0.008936493315992777 2305 7 0.012641852698372702 2305 8 0.042772047808490754 2305 9 0.009280288700168171 2305 10-11 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96-97 -0.2725616612373116 1213 98-99 -0.23216426323050143 1213 100-101 -0.10597283368468169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 4.0 8 65.0 9 207.0 10 322.0 11 221.0 12 158.0 13 230.0 14 457.0 15 974.0 16 1766.0 17 2902.0 18 4257.0 19 5381.0 20 7076.0 21 8942.0 22 11204.0 23 14161.0 24 17735.0 25 22216.0 26 27984.0 27 35755.0 28 46582.0 29 60551.0 30 73615.0 31 90896.0 32 122529.0 33 173302.0 34 264813.0 35 453339.0 36 865446.0 37 1310845.0 38 330556.0 39 778.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.90076714888322 32.22542078688744 15.82734742390574 19.0464646403236 2 30.788416467143836 24.68847891547227 29.947690043916094 14.575414573467803 3 32.568927026473546 23.036075918963814 24.256928098461042 20.138068956101606 4 28.424681004952944 22.839653382529782 28.709541590429456 20.026124022087824 5 31.328784128517146 21.100582261135145 27.985902353063132 19.584731257284584 6 28.440308752626244 21.588589400976417 30.14393454808395 19.827167298313388 7 31.21255944600495 22.359990594826648 24.616650696412634 21.81079926275577 8 27.64248711213141 25.2530674264968 25.28598553322529 21.818459928146495 9 26.690693682100086 22.668717938345548 28.375256303615178 22.265332075939188 10-11 22.004464929069318 29.236335319712687 31.109039332333822 17.650160418884173 12-13 22.053551337835344 28.64068698217821 30.15930644431354 19.146455235672914 14-15 22.077152760747055 28.481759778725603 30.396445754651392 19.044641705875957 16-17 22.342722494292424 27.826343081508973 30.65448123642634 19.176453187772264 18-19 21.607728423091217 27.855645758696625 31.030953639068887 19.505672179143268 20-21 21.96635122254612 28.12609505798593 30.572274459139326 19.335279260328626 22-23 21.578994607194957 28.915977923125347 29.989267483635757 19.51575998604394 24-25 21.333120115693745 28.19281616678507 30.536714307746376 19.937349409774807 26-27 21.28863516270697 28.31557643346725 30.509687075724283 19.886101328101493 28-29 21.320478753662837 29.157137186589033 30.081132261514387 19.44125179823375 30-31 20.702885011642692 29.442869892573704 30.333188379048714 19.52105671673489 32-33 20.54127783943953 29.822932442033036 29.996561549527595 19.63922816899984 34-35 21.02124507302915 29.464827938421394 30.276087346755087 19.237839641794366 36-37 20.615394145026762 28.981359047549223 31.128481747137364 19.274765060286654 38-39 20.94801366278408 29.044338313187218 30.450500218695563 19.557147805333138 40-41 20.7940039491615 29.106104008070243 30.35804129680148 19.741850745966776 42-43 21.09384441517267 29.13195559342346 30.017862244549676 19.756337746854197 44-45 20.641839874395426 28.846817031454236 30.38840584839973 20.122937245750606 46-47 21.022980243811606 29.040580572689528 30.046795829459803 19.88964335403906 48-49 20.675398752763805 29.748201393374956 29.922822045715137 19.653577808146096 50-51 21.156344039941672 29.342490741776633 29.38753194316187 20.113633275119824 52-53 20.697926311206537 29.093473510899898 29.360377065585485 20.84822311230808 54-55 21.257094136862055 29.15696413484053 29.531432663238633 20.054509065058788 56-57 20.949747722115543 28.866878596609762 29.74536293677047 20.438010744504226 58-59 21.011258144346893 29.341483390678064 29.61971933911155 20.027539125863488 60-61 21.01867910414836 29.5300812878629 29.323046975689742 20.128192632298997 62-63 20.598163803865177 29.515629789990992 29.2424189750802 20.643787431063636 64-65 20.859299490569626 29.24250440749879 29.345490381254173 20.552705720677412 66-67 20.97929267897963 29.506048591022193 29.090132097991223 20.42452663200696 68-69 21.0376818477181 29.666139772493494 29.166342652671283 20.129835727117122 70-71 20.965474792566415 30.143634077838975 29.01339083467433 19.877500294920278 72-73 20.658725107399185 30.12981715903778 28.926711135959565 20.284746597603473 74-75 20.81838393790088 29.56576537027128 28.938232149486197 20.677618542341644 76-77 20.88533607911034 29.20721251965077 28.994869115799283 20.9125822854396 78-79 20.56355043397879 28.866062272062525 29.7283688223003 20.84201847165838 80-81 20.54281092827647 29.252702645473217 29.418461108642465 20.786025317607855 82-83 20.11614448254293 29.530388248923078 29.28164265888898 21.071824609645013 84-85 20.137938479999214 29.55475480314495 29.339201434881794 20.968105281974044 86-87 20.29067266730709 29.337591400992597 29.827655221434608 20.544080710265714 88-89 20.42351665560183 29.790588234996633 29.52863546235442 20.257259647047118 90-91 20.719600342286316 29.578205950360392 28.9889910038578 20.713202703495487 92-93 20.377178236048568 29.68660862757474 29.025880397797387 20.910332738579307 94-95 19.973618008332846 29.817314885747365 28.990137160816737 21.218929945103053 96-97 20.041615970093755 30.215275465884805 28.87084218034101 20.87226638368043 98-99 20.31519909057975 29.98149420832577 28.756052949198533 20.947253751895943 100-101 20.39518934233138 29.98732373851854 28.357021986347164 21.26046493280292 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 246.0 1 149.0 2 86.5 3 153.5 4 162.0 5 112.5 6 91.0 7 87.5 8 76.5 9 75.0 10 84.5 11 88.0 12 91.0 13 98.0 14 109.0 15 120.5 16 127.5 17 180.5 18 241.5 19 290.5 20 464.0 21 751.5 22 1194.0 23 1873.0 24 2929.0 25 4657.0 26 7645.5 27 12219.5 28 17193.0 29 22626.5 30 28676.5 31 33192.5 32 40421.5 33 56666.0 34 79825.5 35 112466.5 36 156381.5 37 209666.0 38 262547.0 39 281723.0 40 287790.0 41 276517.0 42 242283.5 43 227069.5 44 213767.5 45 204060.5 46 208710.0 47 194466.0 48 159425.5 49 130500.0 50 105125.5 51 84879.5 52 70256.0 53 56388.0 54 45809.5 55 35370.5 56 24376.0 57 15751.0 58 10383.0 59 7320.5 60 5404.0 61 4032.5 62 2943.5 63 1989.0 64 1347.0 65 960.5 66 714.0 67 542.5 68 402.5 69 280.5 70 208.0 71 146.5 72 96.0 73 70.0 74 54.0 75 42.5 76 32.5 77 24.5 78 18.0 79 13.0 80 10.5 81 9.0 82 4.5 83 2.5 84 2.0 85 1.5 86 0.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1938173626579222 2 0.0 3 0.0 4 0.02667327388522149 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 7.584817218546395E-5 48-49 0.003160340507727665 50-51 6.320681015455328E-5 52-53 0.0024271415099348464 54-55 0.008381223026493767 56-57 0.012742492927157945 58-59 0.013475691924950763 60-61 0.0032488300419440392 62-63 0.002085824735100259 64-65 0.009164987472410228 66-67 0.01592811615894743 68-69 0.024410470081688482 70-71 0.020516930576168 72-73 0.015953398883009253 74-75 0.020681268282569838 76-77 0.0149420899205364 78-79 0.006017288326713473 80-81 0.0029707200772640047 82-83 6.067853774837116E-4 84-85 0.002553555130243953 86-87 0.014689262679918184 88-89 0.013892856871970815 90-91 0.017343948706409422 92-93 0.017546210498903994 94-95 0.017154328275945763 96-97 0.019682600682127897 98-99 0.027874203278158004 100-101 0.016029247055194717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3955270.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.71779548946078 #Duplication Level Percentage of deduplicated Percentage of total 1 87.00580198211651 36.29690253486458 2 5.863754866083706 4.892458526072211 3 1.935076184581852 2.4218133757473526 4 0.9555774717776242 1.5945834216781962 5 0.7234168368578938 1.5089677826835113 6 0.5292001333621333 1.324623776195811 7 0.36030070781388857 1.0521665870301435 8 0.2498742061098833 0.8339360822866786 9 0.23407282779817087 0.8788502123751476 >10 1.7063751269450784 14.328396785135201 >50 0.2271093613121476 6.695158213052621 >100 0.17317425873642786 13.934690112762793 >500 0.025953594041002163 7.691116697043913 >1k 0.01025154880953276 6.074415256821425 >5k 0.0 0.0 >10k+ 6.089365422506581E-5 0.4719206362503853 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT 18577 0.4696771648964546 TruSeq Adapter, Index 2 (97% over 37bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001036591686534674 0.0 0.0 2 0.0012388534790292445 0.0 0.0 3 0.0015422461677711003 0.0 0.0 4 0.0019467697527602414 0.0 0.0 5 0.0023512933377493827 0.0 0.0 6 0.003413167748345878 0.0 0.0 7 0.004196932194262338 0.0 0.0 8 0.005461068397353405 0.0 0.0 9 0.006168984671084401 0.0 0.0 10-11 0.00786292718322643 0.0 0.0 12-13 0.011035909052985005 0.0 0.0 14-15 0.01575113709051468 0.0 0.0 16-17 0.024094436030915715 0.0 0.0 18-19 0.03567392365122988 0.0 0.0 20-21 0.04597663370642206 0.0 0.0 22-23 0.057505555878612585 0.0 0.0 24-25 0.0717523708874489 0.0 0.0 26-27 0.08603710998237794 0.0 0.0 28-29 0.10250880470865453 0.0 0.0 30-31 0.12177424044376238 0.0 0.0 32-33 0.14077420757622108 0.0 0.0 34-35 0.16215075077049101 0.0 0.0 36-37 0.1853982155453357 0.0 0.0 38-39 0.2124254475674227 0.0 0.0 40-41 0.243371501819092 0.0 0.0 42-43 0.2769469593731907 0.0 0.0 44-45 0.3130506893334716 0.0 0.0 46-47 0.3516447676138418 0.0 0.0 48-49 0.3943346471922271 0.0 0.0 50-51 0.44340841459622227 0.0 0.0 52-53 0.49391065590971034 0.0 0.0 54-55 0.548862656658079 0.0 0.0 56-57 0.607012922000268 0.0 0.0 58-59 0.6696129467773375 0.0 0.0 60-61 0.7340333276868583 0.0 0.0 62-63 0.8034596879606196 0.0 0.0 64-65 0.8753511138304085 0.0 0.0 66-67 0.9510349483094707 0.0 0.0 68-69 1.0323694716163498 0.0 0.0 70-71 1.1200120345766535 0.0 0.0 72-73 1.2140005612764742 0.0 0.0 74-75 1.3144108998879975 0.0 0.0 76-77 1.4225324693383765 0.0 0.0 78-79 1.5379860287666833 0.0 0.0 80-81 1.6639951254908008 0.0 0.0 82-83 1.8003195736321413 0.0 0.0 84-85 1.943306019563772 0.0 1.2641362030910658E-5 86-87 2.099388916559426 0.0 5.056544812364263E-5 88-89 2.2674684661224136 0.0 5.056544812364263E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGACCG 25 5.2761672E-5 76.002205 2 CGGGTCG 50 4.1848398E-7 57.1172 1 GTCGACC 25 0.0049815145 57.1172 1 CGACGGG 160 0.0 56.522232 1 CGAGGCG 160 0.0 53.54738 1 CGGAGCG 65 4.352114E-8 51.25903 1 ATCCGAG 100 1.2732926E-11 47.597668 1 CGAACGG 75 1.3420868E-7 44.42449 1 CGAGCGG 90 1.17634045E-8 42.30904 1 TCGCCGG 70 4.3120126E-6 40.71547 2 GCGCACC 190 0.0 40.001163 2 CGCGAGG 60 8.194646E-5 39.664722 1 CGGGACG 50 0.0016000653 38.078133 1 CGTACCG 50 0.0016000653 38.078133 1 CGAGCGC 50 0.0016000653 38.078133 1 TGCCGGC 125 1.3460522E-10 38.001106 2 GTGACGC 140 1.0913936E-11 37.398167 1 CGACTGC 115 2.4556357E-9 37.25035 1 CGAGCCC 180 0.0 37.02041 1 CGAGGGG 425 0.0 36.95819 1 >>END_MODULE