FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_44065-1.341000000357de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_44065-1.341000000357de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955270
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT185770.4696771648964546TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCG255.2761672E-576.0022052
CGGGTCG504.1848398E-757.11721
GTCGACC250.004981514557.11721
CGACGGG1600.056.5222321
CGAGGCG1600.053.547381
CGGAGCG654.352114E-851.259031
ATCCGAG1001.2732926E-1147.5976681
CGAACGG751.3420868E-744.424491
CGAGCGG901.17634045E-842.309041
TCGCCGG704.3120126E-640.715472
GCGCACC1900.040.0011632
CGCGAGG608.194646E-539.6647221
CGGGACG500.001600065338.0781331
CGTACCG500.001600065338.0781331
CGAGCGC500.001600065338.0781331
TGCCGGC1251.3460522E-1038.0011062
GTGACGC1401.0913936E-1137.3981671
CGACTGC1152.4556357E-937.250351
CGAGCCC1800.037.020411
CGAGGGG4250.036.958191
Produced by FastQC (version 0.11.2)