##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_41442-1.34100000035721.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3199754 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69614820389317 33.0 33.0 33.0 33.0 33.0 2 32.71874025315696 33.0 33.0 33.0 33.0 33.0 3 32.75604343333894 33.0 33.0 33.0 33.0 33.0 4 36.676115101348415 37.0 37.0 37.0 37.0 37.0 5 36.67332301170652 37.0 37.0 37.0 37.0 37.0 6 36.55590304754678 37.0 37.0 37.0 37.0 37.0 7 36.42491079001698 37.0 37.0 37.0 37.0 37.0 8 36.37901882457214 37.0 37.0 37.0 37.0 37.0 9 36.420926733742654 37.0 37.0 37.0 37.0 37.0 10-11 36.360866022825505 37.0 37.0 37.0 37.0 37.0 12-13 36.34212958246165 37.0 37.0 37.0 37.0 37.0 14-15 38.167344583364844 40.0 37.0 40.0 37.0 40.0 16-17 38.2019195850681 40.0 37.0 40.0 37.0 40.0 18-19 38.166098393814025 40.0 37.0 40.0 37.0 40.0 20-21 38.14773651349448 40.0 37.0 40.0 37.0 40.0 22-23 37.91575539869628 40.0 37.0 40.0 33.0 40.0 24-25 37.99438738102992 40.0 37.0 40.0 35.0 40.0 26-27 37.92053811011722 40.0 37.0 40.0 33.0 40.0 28-29 37.69641900596109 40.0 37.0 40.0 33.0 40.0 30-31 37.67193759270244 40.0 37.0 40.0 33.0 40.0 32-33 37.552534038554214 40.0 37.0 40.0 33.0 40.0 34-35 37.536548434660915 40.0 37.0 40.0 33.0 40.0 36-37 37.4448185704276 40.0 37.0 40.0 33.0 40.0 38-39 37.18221619537002 40.0 37.0 40.0 33.0 40.0 40-41 37.02404169195506 38.5 37.0 40.0 33.0 40.0 42-43 36.79236325667536 37.0 37.0 40.0 33.0 40.0 44-45 36.572589174042754 37.0 37.0 40.0 33.0 40.0 46-47 36.68495109311529 37.0 37.0 40.0 33.0 40.0 48-49 36.22672336685883 37.0 37.0 40.0 33.0 40.0 50-51 36.15971009021318 37.0 37.0 40.0 33.0 40.0 52-53 36.15570603240124 37.0 37.0 40.0 33.0 40.0 54-55 35.70373847489526 37.0 37.0 40.0 27.0 40.0 56-57 35.58746156735799 37.0 37.0 40.0 27.0 40.0 58-59 35.424220736969154 37.0 33.0 40.0 27.0 40.0 60-61 35.01546040726881 37.0 33.0 40.0 27.0 40.0 62-63 34.771347734857116 37.0 33.0 37.0 27.0 40.0 64-65 34.533068167115346 37.0 33.0 37.0 27.0 40.0 66-67 33.79239669674606 37.0 33.0 37.0 27.0 40.0 68-69 33.79361929073298 37.0 33.0 37.0 27.0 40.0 70-71 33.455731440604495 37.0 33.0 37.0 27.0 38.5 72-73 32.592477109177764 37.0 33.0 37.0 22.0 37.0 74-75 31.6963969730173 35.0 33.0 37.0 18.5 37.0 76-77 29.23777515396496 33.0 27.0 35.0 15.0 37.0 78-79 31.20028070907951 33.0 33.0 37.0 15.0 37.0 80-81 31.47386767857779 35.0 33.0 37.0 15.0 37.0 82-83 31.361842347880494 35.0 33.0 37.0 15.0 37.0 84-85 31.164706880591446 33.0 33.0 37.0 15.0 37.0 86-87 31.06073779421793 33.0 33.0 37.0 15.0 37.0 88-89 30.93467700954511 33.0 33.0 37.0 15.0 37.0 90-91 30.778987540917207 33.0 33.0 37.0 15.0 37.0 92-93 30.64678519036151 33.0 33.0 37.0 15.0 37.0 94-95 30.525115524505946 33.0 33.0 37.0 15.0 37.0 96-97 30.360445678011494 33.0 27.0 37.0 15.0 37.0 98-99 30.19101749697008 33.0 27.0 37.0 15.0 37.0 100-101 28.623826550416062 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10171377962198846 2306 2 0.10064042927309202 2306 3 0.1369604900530561 2306 4 0.1529536370574789 2306 5 0.14594714029841072 2306 6 0.23474773636274904 2306 7 0.3185009552942475 2306 8 0.2695175190235446 2306 9 0.30786469206989864 2306 10-11 0.2978562479967053 2306 12-13 0.274469101574077 2306 14-15 0.45354600314040283 2306 16-17 0.4617094490078131 2306 18-19 0.37413067333921646 2306 20-21 0.47181956200186903 2306 22-23 0.4840002610864218 2306 24-25 0.3920226135802878 2306 26-27 0.5574613980778338 2306 28-29 0.5215287308605312 2306 30-31 0.596739385970281 2306 32-33 0.5486453116654104 2306 34-35 0.606599931532763 2306 36-37 0.5923311805394533 2306 38-39 0.6361166702674552 2306 40-41 0.5417575739563887 2306 42-43 0.5434717426329598 2306 44-45 0.5688228610613351 2306 46-47 0.38213680622465773 2306 48-49 0.29857489178949237 2306 50-51 0.20754943274943827 2306 52-53 0.37515639441621573 2306 54-55 0.2295761691371112 2306 56-57 0.3122802456978704 2306 58-59 0.4640951731371601 2306 60-61 0.12121721841774047 2306 62-63 0.1250385782465102 2306 64-65 0.22384361929983498 2306 66-67 0.3641937926044605 2306 68-69 0.6675400281375516 2306 70-71 0.6912049767250323 2306 72-73 0.33914908113600717 2306 74-75 0.419210760282116 2306 76-77 0.5164259207525994 2306 78-79 0.5317962989486027 2306 80-81 0.4180668318969474 2306 82-83 0.5712643001737909 2306 84-85 0.7066000719587358 2306 86-87 0.5326892951139364 2306 88-89 0.563582284643978 2306 90-91 0.4529341700338172 2306 92-93 0.6876502281988301 2306 94-95 0.46969026403818503 2306 96-97 0.7027960763762273 2306 98-99 0.7387747657427646 2306 100-101 0.5110100502671067 2307 1 0.12366288045116391 2307 2 0.12315174903126547 2307 3 0.12556042954427227 2307 4 0.1679589956745957 2307 5 0.1662542993083207 2307 6 0.17830206351016642 2307 7 0.13909753090715782 2307 8 0.18905662667278023 2307 9 0.2366187751310278 2307 10-11 0.2227011557720857 2307 12-13 0.2047256485735076 2307 14-15 0.3915578279931111 2307 16-17 0.46222582483916597 2307 18-19 0.43303125945399046 2307 20-21 0.4539491636181765 2307 22-23 0.5035909550401598 2307 24-25 0.5116548432265162 2307 26-27 0.45305297402405387 2307 28-29 0.4719766497172273 2307 30-31 0.46921465684965824 2307 32-33 0.596870712023339 2307 34-35 0.5390989956977208 2307 36-37 0.42489044581920155 2307 38-39 0.5774988345334293 2307 40-41 0.5347160946703937 2307 42-43 0.6819373833851117 2307 44-45 0.5286408219152321 2307 46-47 0.5090794203067972 2307 48-49 0.32874467534237795 2307 50-51 0.34270083291250586 2307 52-53 0.34166867821267743 2307 54-55 0.1436092848781101 2307 56-57 0.34077362819032686 2307 58-59 0.2941322475886139 2307 60-61 0.19705852130524448 2307 62-63 0.17646632921696437 2307 64-65 0.16824599247495797 2307 66-67 0.152098701507974 2307 68-69 0.5477899601933629 2307 70-71 0.4929282464557687 2307 72-73 0.25052893786351405 2307 74-75 0.2867796915841261 2307 76-77 0.3353272247682284 2307 78-79 0.4730667783525391 2307 80-81 0.39298470916666517 2307 82-83 0.21600254839314204 2307 84-85 0.33158425250054435 2307 86-87 0.363350225410894 2307 88-89 0.3072202985199759 2307 90-91 0.2936518657771785 2307 92-93 0.4372451782790847 2307 94-95 0.21240854435795598 2307 96-97 0.3620981741850926 2307 98-99 0.4407865290611106 2307 100-101 0.11528986751006087 2201 1 0.12090406302279888 2201 2 0.08207478439165783 2201 3 0.06242029340759814 2201 4 0.15105658673937228 2201 5 0.0873467932770069 2201 6 0.12478822219270569 2201 7 0.14238759497036568 2201 8 0.1493555757037086 2201 9 0.18713594713062776 2201 10-11 0.2553114243992525 2201 12-13 0.18890519179964116 2201 14-15 0.38070042766324974 2201 16-17 0.4061571066133709 2201 18-19 0.36573853915761134 2201 20-21 0.43762060306607253 2201 22-23 0.5183958654820273 2201 24-25 0.4998421624524738 2201 26-27 0.4816661407036378 2201 28-29 0.4419451565401147 2201 30-31 0.46120439464581864 2201 32-33 0.4742612746497983 2201 34-35 0.5473575949220049 2201 36-37 0.43853707990324864 2201 38-39 0.5287019796948726 2201 40-41 0.5518269782363134 2201 42-43 0.4781738825982558 2201 44-45 0.48648625030367043 2201 46-47 0.5576891486191045 2201 48-49 0.5420971589959649 2201 50-51 0.7024250834145604 2201 52-53 0.6995230803618497 2201 54-55 0.657927817488762 2201 56-57 0.8155422468546121 2201 58-59 0.6237886375674719 2201 60-61 0.5004653387184987 2201 62-63 0.6207817824107735 2201 64-65 0.5491009935045739 2201 66-67 0.46425162950441035 2201 68-69 0.7192982698957877 2201 70-71 0.5020667465341617 2201 72-73 0.44719419391795867 2201 74-75 0.31473418422659094 2201 76-77 0.17050342219852155 2201 78-79 0.5977534996426463 2201 80-81 0.7270778209079403 2201 82-83 0.5571925824178656 2201 84-85 0.5524763009928577 2201 86-87 0.6978078607588181 2201 88-89 0.7351496646324094 2201 90-91 0.6980411682987118 2201 92-93 0.6458225821606582 2201 94-95 0.6112807787865933 2201 96-97 0.570632976318393 2201 98-99 0.5074329496641106 2201 100-101 0.7170308715510849 1101 1 0.05251264177677939 1101 2 0.07017513185864743 1101 3 0.08179734811025696 1101 4 0.15305218257974929 1101 5 0.15434772276678643 1101 6 0.1747607741723911 1101 7 0.20065693490785463 1101 8 0.22357938935660115 1101 9 0.18230228837190054 1101 10-11 0.15884551296902316 1101 12-13 0.1885873562008058 1101 14-15 0.3225879781145835 1101 16-17 0.2612357701443031 1101 18-19 0.33397743674530034 1101 20-21 0.3574101578467008 1101 22-23 0.3285060942363245 1101 24-25 0.38111606250424757 1101 26-27 0.31954253727761994 1101 28-29 0.36894995370548855 1101 30-31 0.39320114455068733 1101 32-33 0.3610522267841887 1101 34-35 0.3726651375132306 1101 36-37 0.4408422457454364 1101 38-39 0.5179815003526187 1101 40-41 0.503981959740095 1101 42-43 0.5036437739914277 1101 44-45 0.6318681208862529 1101 46-47 0.6829530239052843 1101 48-49 0.5987704589342044 1101 50-51 0.6367818952022404 1101 52-53 0.5671936791653636 1101 54-55 0.6200352016300457 1101 56-57 0.7457525361193191 1101 58-59 0.7101920423696271 1101 60-61 0.6673591273562636 1101 62-63 0.7411312144238948 1101 64-65 0.705879718793561 1101 66-67 0.7037119016140991 1101 68-69 0.6605214534918531 1101 70-71 0.5897687857973111 1101 72-73 0.4145683229111867 1101 74-75 0.39021577221932446 1101 76-77 0.2648948247299785 1101 78-79 0.474603745739099 1101 80-81 0.6284942011378725 1101 82-83 0.4611243553728315 1101 84-85 0.54274223488056 1101 86-87 0.6885988939896386 1101 88-89 0.5572443852552311 1101 90-91 0.6320785849409418 1101 92-93 0.6465566607493329 1101 94-95 0.42615261524629844 1101 96-97 0.5134433104980367 1101 98-99 0.6245912374066975 1101 100-101 0.5703542653740321 2304 1 0.07791495428127604 2304 2 0.14168019010529775 2304 3 0.0893804348228926 2304 4 0.1598437225094571 2304 5 0.13876949955515983 2304 6 0.1405263758107722 2304 7 0.130812068939683 2304 8 0.14600266471551038 2304 9 0.12088174173631927 2304 10-11 0.14421203453781573 2304 12-13 0.06771570671097038 2304 14-15 0.21169204538578157 2304 16-17 0.22669083530127665 2304 18-19 0.20997643617533868 2304 20-21 0.25467026948564353 2304 22-23 0.27613093989612025 2304 24-25 0.23988606962017656 2304 26-27 0.3679722363870468 2304 28-29 0.3690193425468422 2304 30-31 0.3385494365572015 2304 32-33 0.28700005551311136 2304 34-35 0.26347779792138226 2304 36-37 0.31618719109672355 2304 38-39 0.31903955168552045 2304 40-41 0.30913723654732195 2304 42-43 0.3296079933355571 2304 44-45 0.45370413249560926 2304 46-47 0.4750182242876946 2304 48-49 0.6199097837322398 2304 50-51 0.6119335512254764 2304 52-53 0.655248118851901 2304 54-55 0.6012362510862985 2304 56-57 0.5062105481604178 2304 58-59 0.4515185799749517 2304 60-61 0.5430495891604181 2304 62-63 0.6212094496286582 2304 64-65 0.43147255078347513 2304 66-67 0.40553577160397225 2304 68-69 0.48906219759152236 2304 70-71 0.3758821083406403 2304 72-73 0.004723988067475204 2304 74-75 0.08265058954106763 2304 76-77 -0.04865321804781075 2304 78-79 0.16065764516354264 2304 80-81 0.25862540601371364 2304 82-83 0.21950084494851652 2304 84-85 0.24053013004001755 2304 86-87 0.2606948995910052 2304 88-89 0.1701014807925283 2304 90-91 0.2305153657098984 2304 92-93 0.27754123415358833 2304 94-95 0.15252785639393807 2304 96-97 0.202571164149834 2304 98-99 0.27276054275300865 2304 100-101 0.25614531529859264 2202 1 0.0812713426814966 2202 2 0.07764397047897376 2202 3 0.008484918931571883 2202 4 0.07724864166376477 2202 5 0.08639672961959377 2202 6 0.01727664714068311 2202 7 0.06465752246248968 2202 8 0.096274886170022 2202 9 0.1410730401420608 2202 10-11 0.1311082877857075 2202 12-13 0.0813691276180677 2202 14-15 0.1179885644701244 2202 16-17 0.1021441116214632 2202 18-19 0.12023795127528558 2202 20-21 0.18742325791684777 2202 22-23 0.13862252598105584 2202 24-25 0.13451848346915796 2202 26-27 0.10492171985722365 2202 28-29 0.07568974494372327 2202 30-31 0.1076128704624395 2202 32-33 0.1315162150810636 2202 34-35 0.1315736348722254 2202 36-37 0.17976639124314175 2202 38-39 0.13395983877209972 2202 40-41 0.18995948877309132 2202 42-43 0.14236662207258632 2202 44-45 0.15539278918768673 2202 46-47 0.26716594051449505 2202 48-49 0.4331834738688869 2202 50-51 0.4035826602223551 2202 52-53 0.2744280954777949 2202 54-55 0.5730158423044145 2202 56-57 0.4467202026281001 2202 58-59 0.36191828387855907 2202 60-61 0.34799845274761765 2202 62-63 0.4247412879881338 2202 64-65 0.33624040539312006 2202 66-67 0.41006348711228924 2202 68-69 0.3519218719746533 2202 70-71 0.18125943588454874 2202 72-73 0.05179005883535126 2202 74-75 0.06892905754904533 2202 76-77 -0.10863928641377285 2202 78-79 0.08606001188935508 2202 80-81 0.2406556827348254 2202 82-83 0.3371900475781402 2202 84-85 0.23685409808824787 2202 86-87 0.14678733714972125 2202 88-89 0.14510628593451713 2202 90-91 0.14382817874883713 2202 92-93 0.09069325521073068 2202 94-95 0.0828303535206878 2202 96-97 0.23388646458723983 2202 98-99 0.29386317927476213 2202 100-101 0.2867377466951915 1102 1 0.057138674346440155 1102 2 0.020660905226925763 1102 3 0.040962941189135904 1102 4 0.08771037301108464 1102 5 0.06479779661250973 1102 6 0.08680642911507874 1102 7 0.09092268521342817 1102 8 0.01042929680713911 1102 9 0.037154049431343594 1102 10-11 0.12891042333514235 1102 12-13 0.04882050331752197 1102 14-15 0.13276477824493327 1102 16-17 0.07190184077109762 1102 18-19 0.07989928758793496 1102 20-21 0.030749506589756948 1102 22-23 0.10818348350833418 1102 24-25 0.04567663721731208 1102 26-27 -0.014484676928418594 1102 28-29 0.04368573061018566 1102 30-31 0.06318848940000521 1102 32-33 0.07607845655520151 1102 34-35 -0.007683241941158769 1102 36-37 0.017814626029696967 1102 38-39 -0.0775614163518128 1102 40-41 -0.030674842318255457 1102 42-43 0.013793566337980678 1102 44-45 0.06682481150803454 1102 46-47 0.20157483844065638 1102 48-49 0.1866297135403343 1102 50-51 0.1265493243912843 1102 52-53 0.1807470023801656 1102 54-55 0.2553358158148171 1102 56-57 0.1849069593446515 1102 58-59 0.21484616984531613 1102 60-61 0.20258103651056558 1102 62-63 0.19877241326562967 1102 64-65 0.1445829208007794 1102 66-67 0.2028107689637082 1102 68-69 0.14469387055490301 1102 70-71 -0.0790775391270202 1102 72-73 -0.2777972367419679 1102 74-75 -0.35450140310703304 1102 76-77 -0.4242020334081644 1102 78-79 -0.343575911296103 1102 80-81 -0.13939878307291664 1102 82-83 -0.263614394745705 1102 84-85 -0.25662922509578934 1102 86-87 -0.3025833003626701 1102 88-89 -0.2453170581077977 1102 90-91 -0.3087430646661993 1102 92-93 -0.45088334974991184 1102 94-95 -0.7828154621295624 1102 96-97 -0.4932901019812874 1102 98-99 -0.21802941613451665 1102 100-101 -0.3543473261205321 2305 1 0.017312366697282755 2305 2 0.013080483437626356 2305 3 0.03293612327348683 2305 4 0.07863844448658597 2305 5 0.06919919052766232 2305 6 -0.018467683829875625 2305 7 0.00648855408462623 2305 8 -0.03209717945933477 2305 9 0.014002782588562468 2305 10-11 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90-91 0.22093036378420194 1213 92-93 0.007847702977308302 1213 94-95 0.2994471551917215 1213 96-97 0.24213259181609814 1213 98-99 0.3681929825352874 1213 100-101 0.4409480452330321 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 25.0 10 20.0 11 29.0 12 43.0 13 153.0 14 490.0 15 1251.0 16 2864.0 17 4754.0 18 6048.0 19 7273.0 20 9474.0 21 12554.0 22 17069.0 23 22651.0 24 28825.0 25 35889.0 26 47010.0 27 67126.0 28 102195.0 29 117243.0 30 79789.0 31 70555.0 32 92881.0 33 131011.0 34 198917.0 35 341631.0 36 655926.0 37 944081.0 38 201808.0 39 166.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.811450989969586 23.670597739046304 21.915783310398165 13.60216796058595 2 27.794105421854304 28.968289437250487 30.598946043977133 12.638659096918076 3 34.26541540380917 17.778929255186494 32.61544481231995 15.340210528684391 4 27.287439495734223 17.485654658969136 30.918601990404344 24.308303854892298 5 36.48592985585767 16.271563376434564 31.67596633991238 15.566540427795386 6 32.11896914575308 17.030027933397378 35.128981790475144 15.722021130374397 7 30.224542261686366 26.264206560879366 26.453096081761284 17.05815509567298 8 26.96491667796962 33.795629289001596 22.81109735310902 16.42835667991977 9 34.89952665111131 23.502056720610398 23.916807354565382 17.681609273712915 10-11 26.42675030642981 29.966991212449457 27.1031148019504 16.503143679170336 12-13 21.824584014896146 28.627263220860105 27.104130505032575 22.444022259211177 14-15 20.980753520426884 29.2277625092429 27.931912890803478 21.859571079526738 16-17 24.649098024410627 25.61273460397268 28.52666173712104 21.21150563449565 18-19 19.46757469480466 25.660363265426028 33.12728103472954 21.744781005039762 20-21 24.187015626826312 25.999108056431837 32.5151871050087 17.29868921173315 22-23 19.57030134191566 34.62375545120031 28.304535286150124 17.501407920733904 24-25 19.942642465639544 25.27927146899418 32.72532825960996 22.05275780575632 26-27 19.769097874399094 25.462973091056373 28.114801950399936 26.653127084144597 28-29 23.724667583820505 30.218651183809754 27.880596445851776 18.17608478651796 30-31 18.809227209341717 26.436469803616152 32.09840506488936 22.655897922152764 32-33 18.513720117234012 30.961208267885592 28.345772831286403 22.17929878359399 34-35 18.904437653644624 26.481863918288717 32.84743139628859 21.76626703177807 36-37 23.411815408309515 27.067049529432573 28.27262658316858 21.24850847908933 38-39 24.00028252171886 31.24760528465626 27.369760300323087 17.3823518933018 40-41 23.740075018267028 30.945644571426428 27.219279982148624 18.095000428157913 42-43 19.048229957677997 30.970912138870677 32.167175976653205 17.81368192679812 44-45 18.70157830883249 26.629609651241935 32.55250247362766 22.116309566297907 46-47 23.51938157716886 26.256452647509455 27.980679755699857 22.24348601962183 48-49 19.418430322557054 31.292506588999863 31.440303958150434 17.848759130292645 50-51 23.783690702231095 26.700557840194776 31.07150664668803 18.444244810886097 52-53 19.003996197728732 26.63799492853396 26.90105359417927 27.45695527955804 54-55 23.809979243378805 26.620483887754844 31.04777648485167 18.521760384014684 56-57 19.328671416102292 26.693516059234323 30.946550486000575 23.03126203866281 58-59 19.212806860465353 26.58603798655119 31.65174498990644 22.54941016307702 60-61 23.12673622532718 26.847503140578365 31.673422354542268 18.352338279552182 62-63 18.909277347839755 26.89113869298425 31.2740218883978 22.925562070778195 64-65 23.317558712563404 26.73379397601395 31.18102119650202 18.76762611492063 66-67 19.474130947917438 35.01708307825802 26.573349085096876 18.93543688872767 68-69 19.516459483842493 35.55375739384159 26.419998962158157 18.509784160157754 70-71 18.88708836955159 36.38361940022075 26.330125845673614 18.399166384554043 72-73 18.79415321629055 36.0371045648811 26.31091741166387 18.857824807164477 74-75 19.534737094151964 34.81469475688739 26.21062414018061 19.439944008780046 76-77 19.899973837724502 33.60470833438826 26.6233504403983 19.87196738748893 78-79 19.67223157164041 33.25920822191777 27.217303207596206 19.851256998845617 80-81 19.078217022186273 33.27822289785463 27.156698449448623 20.486861630510468 82-83 18.548266370975327 32.75693628187102 27.348906924421506 21.345890422732147 84-85 18.754938991297998 32.03443233849528 27.817195948390243 21.393432721816477 86-87 18.829959946962703 31.986760680132704 28.386566510109535 20.79671286279506 88-89 19.18247441901753 32.36088638750877 27.842726871076973 20.613912322396725 90-91 19.66518342408823 31.763282173941516 27.477393066889388 21.094141335080863 92-93 19.075228887492692 32.27923180074832 27.520403729717398 21.125135582041594 94-95 18.839136862363688 32.62732095967461 27.502333794960816 21.031208383000877 96-97 19.35641978988281 32.97005629205161 26.8370093708597 20.83651454720588 98-99 19.84771638665839 32.07287405348323 26.536200378012737 21.543209181845636 100-101 19.73108579673397 32.09403745144153 26.18825244527131 21.98662430655319 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1029.0 1 682.0 2 338.5 3 473.0 4 545.5 5 441.0 6 426.5 7 498.5 8 515.5 9 509.5 10 527.5 11 533.0 12 514.5 13 489.0 14 514.5 15 535.5 16 565.5 17 597.5 18 632.0 19 692.0 20 752.5 21 845.0 22 998.5 23 1295.0 24 1677.0 25 2304.5 26 3723.0 27 6137.5 28 8518.0 29 10614.0 30 13151.0 31 15522.5 32 18989.0 33 28627.0 34 46727.0 35 78988.0 36 120451.0 37 165560.5 38 216432.5 39 249823.0 40 260601.5 41 246720.0 42 222608.5 43 216056.5 44 205106.5 45 185932.5 46 175509.0 47 154826.5 48 119734.0 49 92973.0 50 73516.0 51 59625.0 52 49638.5 53 38787.5 54 29103.0 55 20981.0 56 14346.5 57 9357.5 58 6409.5 59 4501.5 60 3276.0 61 2418.5 62 1694.5 63 1210.5 64 854.5 65 639.5 66 463.5 67 307.5 68 231.0 69 173.5 70 119.0 71 88.5 72 66.0 73 49.0 74 34.0 75 20.0 76 17.5 77 13.5 78 12.5 79 14.0 80 11.5 81 6.5 82 4.0 83 2.0 84 4.0 85 4.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1865455906922845 2 0.0 3 0.0 4 0.025189436437926163 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.687860379266656E-5 48-49 0.00321899746042977 50-51 7.813100632111093E-5 52-53 0.0027345852212388826 54-55 0.009156953940834201 56-57 0.013329149678381527 58-59 0.013672926106194413 60-61 0.003265876064222437 62-63 0.002125163371934217 64-65 0.0097194971863462 66-67 0.016735661553981963 68-69 0.025251941242983057 70-71 0.02115787651175684 72-73 0.016407511327433295 74-75 0.02128288612187062 76-77 0.01534492964146619 78-79 0.006516125927180652 80-81 0.003687783498356436 82-83 6.563004530973319E-4 84-85 0.0027345852212388826 86-87 0.015501191654108409 88-89 0.014172964546649524 90-91 0.01779824323994907 92-93 0.018251403076611515 94-95 0.017673233629835295 96-97 0.019829649404297954 98-99 0.02875221032616882 100-101 0.016188744509734187 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3199754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.65853996849875 #Duplication Level Percentage of deduplicated Percentage of total 1 86.09650915977349 32.422688313415485 2 6.305764216518045 4.74931747559348 3 2.092328892562657 2.363821536834466 4 1.1301128232838713 1.7023359569819454 5 0.6921664004461773 1.303298802802714 6 0.5777304058631341 1.3053890148128304 7 0.3766550128108071 0.9928994497989831 8 0.29741785218948597 0.8960257659218249 9 0.2269669161797807 0.769251841603486 >10 1.7678480634777145 12.955366100184209 >50 0.22491378818726626 5.881764457986327 >100 0.17503905584942386 14.573232665736956 >500 0.029132948249719785 7.451017181306575 >1k 0.006916559680690592 4.005576428874578 >5k 4.1492077543919803E-4 0.9178932427940151 >10k+ 8.298415240491877E-5 7.710121765352324 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT 246719 7.710561499415268 TruSeq Adapter, Index 6 (97% over 36bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7792 0.24351872050163856 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT 5695 0.17798243239949071 TruSeq Adapter, Index 6 (97% over 36bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 5560 0.17376335805815074 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 5295 0.16548147138811298 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 5030 0.1571995847180752 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 4529 0.14154213105132457 No Hit GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA 3698 0.1155713845501873 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3476 0.10863335118887264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.004312831548925323 0.0 0.0 2 0.004937879599494211 0.0 0.0 3 0.005719189662705321 0.0 0.0 4 0.006281732908217319 0.0 0.0 5 0.007344314594184428 0.0 0.0 6 0.008313139072566204 0.0 0.0 7 0.009875759198988423 0.0 3.125240252844437E-5 8 0.011250864910239975 0.0 3.125240252844437E-5 9 0.012782232634133748 0.0 3.125240252844437E-5 10-11 0.021626662549683505 0.0 3.125240252844437E-5 12-13 0.03036170905638371 0.0 3.125240252844437E-5 14-15 0.041612573966623684 0.0 3.125240252844437E-5 16-17 0.05600430533097232 0.0 3.125240252844437E-5 18-19 0.0734587721431085 0.0 3.125240252844437E-5 20-21 0.08428772961921448 0.0 3.125240252844437E-5 22-23 0.09456977005107267 0.0 3.125240252844437E-5 24-25 0.1062112899929182 0.0 3.125240252844437E-5 26-27 0.11822783876510506 0.0 3.125240252844437E-5 28-29 0.13126009061946636 0.0 3.125240252844437E-5 30-31 0.22387658551251127 0.0 3.125240252844437E-5 32-33 0.24131542612338325 0.0 3.125240252844437E-5 34-35 0.25505085703463454 0.0 3.125240252844437E-5 36-37 0.2681143612915243 0.0 3.125240252844437E-5 38-39 0.28253734505840133 0.0 3.125240252844437E-5 40-41 0.2986323323605502 0.0 3.125240252844437E-5 42-43 0.31463356245511376 0.0 3.125240252844437E-5 44-45 0.3323380484874775 0.0 3.125240252844437E-5 46-47 0.3511051162058083 0.0 3.125240252844437E-5 48-49 0.3712941682391834 0.0 3.125240252844437E-5 50-51 0.3923582875433549 0.0 3.125240252844437E-5 52-53 0.4168757973269195 0.0 3.125240252844437E-5 54-55 0.44301843204196323 0.0 3.125240252844437E-5 56-57 0.4719581567833027 0.0 3.125240252844437E-5 58-59 0.5041325051863361 0.0 3.125240252844437E-5 60-61 0.5377757165082065 0.0 3.125240252844437E-5 62-63 0.573106557566613 0.0 3.125240252844437E-5 64-65 0.6115470126765995 0.0 4.687860379266656E-5 66-67 0.6516594713218579 0.0 6.250480505688874E-5 68-69 0.6965222951514398 0.0 6.250480505688874E-5 70-71 0.7457604553350039 0.0 6.250480505688874E-5 72-73 0.7994833352813997 0.0 6.250480505688874E-5 74-75 0.8568471201223594 0.0 6.250480505688874E-5 76-77 0.9188675129400572 0.0 7.813100632111093E-5 78-79 0.9869664980495376 0.0 9.375720758533312E-5 80-81 1.061472225677349 0.0 9.375720758533312E-5 82-83 1.1440879517612916 0.0 9.375720758533312E-5 84-85 1.2319853338725415 0.0 9.375720758533312E-5 86-87 1.3249143527908709 0.0 9.375720758533312E-5 88-89 1.4288442173992126 0.0 1.093834088495553E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 35430 0.0 78.99609 3 CGGAAGA 35365 0.0 78.99354 4 GAAGAGC 35380 0.0 78.76951 6 GATCGGA 35690 0.0 78.63464 1 ATCGGAA 35835 0.0 78.1033 2 GGAAGAG 36580 0.0 76.3283 5 GTGCTCG 20 0.0020586425 71.38724 1 GAGCACA 43700 0.0 63.696575 9 AGAGCAC 43745 0.0 63.685337 8 GGTGCCG 30 1.3012755E-4 63.336315 3 AAGAGCA 44350 0.0 62.752323 7 CGATCGC 40 7.462915E-6 59.489365 1 CCGCGTG 25 0.0049839853 57.109795 1 GTGCCGA 25 0.0049839853 57.109795 1 CGGTCGC 55 8.0872996E-7 51.917995 1 TCGGACG 95 7.2759576E-12 50.002354 3 GCGTGCC 80 4.2273314E-9 47.502235 3 GCCGTCT 29660 0.0 45.168434 50-51 CGTCTTC 29720 0.0 45.031765 52-53 ATGCCGT 29645 0.0 45.02165 48-49 >>END_MODULE