FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_41442-1.34100000035721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_41442-1.34100000035721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199754
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2467197.710561499415268TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.24351872050163856No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATATCGTAT56950.17798243239949071TruSeq Adapter, Index 6 (97% over 36bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC55600.17376335805815074No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC52950.16548147138811298No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG50300.1571995847180752No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG45290.14154213105132457No Hit
GTGTTTGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAA36980.1155713845501873No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34760.10863335118887264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG354300.078.996093
CGGAAGA353650.078.993544
GAAGAGC353800.078.769516
GATCGGA356900.078.634641
ATCGGAA358350.078.10332
GGAAGAG365800.076.32835
GTGCTCG200.002058642571.387241
GAGCACA437000.063.6965759
AGAGCAC437450.063.6853378
GGTGCCG301.3012755E-463.3363153
AAGAGCA443500.062.7523237
CGATCGC407.462915E-659.4893651
CCGCGTG250.004983985357.1097951
GTGCCGA250.004983985357.1097951
CGGTCGC558.0872996E-751.9179951
TCGGACG957.2759576E-1250.0023543
GCGTGCC804.2273314E-947.5022353
GCCGTCT296600.045.16843450-51
CGTCTTC297200.045.03176552-53
ATGCCGT296450.045.0216548-49
Produced by FastQC (version 0.11.2)