##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_40825-1.341000000356f9.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1886966 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.660547672825054 33.0 33.0 33.0 33.0 33.0 2 32.682017588022255 33.0 33.0 33.0 33.0 33.0 3 32.71887092825202 33.0 33.0 33.0 33.0 33.0 4 36.64686168166252 37.0 37.0 37.0 37.0 37.0 5 36.64813091491845 37.0 37.0 37.0 37.0 37.0 6 36.521205999472166 37.0 37.0 37.0 37.0 37.0 7 36.42259902934128 37.0 37.0 37.0 37.0 37.0 8 36.33756093114555 37.0 37.0 37.0 37.0 37.0 9 36.38122626480816 37.0 37.0 37.0 37.0 37.0 10-11 36.31315800072709 37.0 37.0 37.0 37.0 37.0 12-13 36.29722236648673 37.0 37.0 37.0 35.0 37.0 14-15 38.088375731200244 40.0 37.0 40.0 35.0 40.0 16-17 38.02291509226981 40.0 37.0 40.0 35.0 40.0 18-19 37.922666598126305 40.0 37.0 40.0 33.0 40.0 20-21 37.859538009693864 40.0 37.0 40.0 33.0 40.0 22-23 37.64794914163795 40.0 37.0 40.0 33.0 40.0 24-25 37.61534627544958 40.0 37.0 40.0 33.0 40.0 26-27 37.475810639937336 40.0 37.0 40.0 33.0 40.0 28-29 37.25348098481902 40.0 37.0 40.0 33.0 40.0 30-31 37.14453864033587 40.0 37.0 40.0 33.0 40.0 32-33 36.98797991060782 37.0 37.0 40.0 33.0 40.0 34-35 36.7886162230798 37.0 37.0 40.0 33.0 40.0 36-37 36.66233519840846 37.0 37.0 40.0 33.0 40.0 38-39 36.53740263470566 37.0 37.0 40.0 33.0 40.0 40-41 36.40361193577415 37.0 37.0 40.0 33.0 40.0 42-43 36.03789098478722 37.0 37.0 40.0 33.0 40.0 44-45 35.82292155767513 37.0 37.0 40.0 30.0 40.0 46-47 35.81293542119995 37.0 37.0 40.0 30.0 40.0 48-49 35.501433253169374 37.0 35.0 40.0 27.0 40.0 50-51 35.308746951455404 37.0 33.0 40.0 27.0 40.0 52-53 35.100140384087474 37.0 33.0 40.0 27.0 40.0 54-55 34.78477540135859 37.0 33.0 40.0 27.0 40.0 56-57 34.55143494901339 37.0 33.0 37.0 27.0 40.0 58-59 34.27513479310173 37.0 33.0 37.0 27.0 40.0 60-61 33.99116624252901 37.0 33.0 37.0 27.0 40.0 62-63 33.652810649476464 37.0 33.0 37.0 27.0 40.0 64-65 33.38108794753059 37.0 33.0 37.0 27.0 38.5 66-67 32.897789361334546 37.0 33.0 37.0 22.0 37.0 68-69 32.623478907410096 37.0 33.0 37.0 22.0 37.0 70-71 32.24552694643147 33.0 33.0 37.0 22.0 37.0 72-73 31.640681920076993 33.0 33.0 37.0 22.0 37.0 74-75 31.14505931744398 33.0 30.0 37.0 15.0 37.0 76-77 28.441795718629802 30.0 24.5 33.0 15.0 35.0 78-79 30.896455739001127 33.0 30.0 37.0 15.0 37.0 80-81 31.369072627699705 33.0 33.0 37.0 15.0 37.0 82-83 31.265367261519287 33.0 33.0 37.0 15.0 37.0 84-85 31.11444456338906 33.0 33.0 37.0 15.0 37.0 86-87 31.009869812174678 33.0 33.0 37.0 15.0 37.0 88-89 30.846165484698716 33.0 33.0 37.0 15.0 37.0 90-91 30.700900016216508 33.0 30.0 37.0 15.0 37.0 92-93 30.563410257524513 33.0 27.0 37.0 15.0 37.0 94-95 30.410692084542063 33.0 27.0 37.0 15.0 37.0 96-97 30.200501757848315 33.0 27.0 37.0 15.0 37.0 98-99 30.00339645759383 33.0 27.0 37.0 15.0 37.0 100-101 28.160625840635177 33.0 24.5 35.0 10.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.040659261744984576 2306 2 0.10324956575797017 2306 3 0.13029310258438898 2306 4 0.1408415363758948 2306 5 0.1576120760420352 2306 6 0.2618640298191437 2306 7 0.16942199589613693 2306 8 0.12235977023088651 2306 9 0.1651058949236628 2306 10-11 0.25392771960865446 2306 12-13 0.28373704815484047 2306 14-15 0.4039671355675054 2306 16-17 0.43033310296552685 2306 18-19 0.4989974388708518 2306 20-21 0.583385762949149 2306 22-23 0.5420848104600324 2306 24-25 0.4807487225460676 2306 26-27 0.6213285606735113 2306 28-29 0.6373935886123974 2306 30-31 0.6429532278928249 2306 32-33 0.770604653504833 2306 34-35 0.7776876002293633 2306 36-37 0.7051163009758241 2306 38-39 0.8631277326464684 2306 40-41 0.8479023988527032 2306 42-43 0.85539283400297 2306 44-45 0.8047579927493231 2306 46-47 0.7381702728382038 2306 48-49 1.020267574836879 2306 50-51 0.7443435634690658 2306 52-53 0.6571748129206014 2306 54-55 0.8449132206201 2306 56-57 0.7409425940742693 2306 58-59 0.6648092440937674 2306 60-61 0.6060317718157648 2306 62-63 0.863140726454219 2306 64-65 0.7575913791800488 2306 66-67 0.7256220237339264 2306 68-69 0.9238196510396435 2306 70-71 0.6469946395391801 2306 72-73 0.7622094545658058 2306 74-75 0.6174496692404325 2306 76-77 0.5865969981680017 2306 78-79 0.7854497244767451 2306 80-81 0.8950201021485817 2306 82-83 0.876590940402135 2306 84-85 0.8424299501444636 2306 86-87 0.9452532298866458 2306 88-89 1.1272655455335467 2306 90-91 1.1279964859579685 2306 92-93 1.0437138181883512 2306 94-95 0.9266188554221522 2306 96-97 1.043478728217483 2306 98-99 1.214163014854467 2306 100-101 0.8674203234146667 2307 1 0.13448029815415197 2307 2 0.0712027539683362 2307 3 0.14307002417945824 2307 4 0.1804061674681705 2307 5 0.09491702691855863 2307 6 0.19662708125432005 2307 7 0.2272239608354525 2307 8 0.2273347268866388 2307 9 0.27249753429950374 2307 10-11 0.20408905653795273 2307 12-13 0.2478889456697715 2307 14-15 0.4905496822829818 2307 16-17 0.5398235672305987 2307 18-19 0.5398387989170814 2307 20-21 0.5364762079501162 2307 22-23 0.6244725970136784 2307 24-25 0.534664634701727 2307 26-27 0.49818321295630597 2307 28-29 0.4861028931779927 2307 30-31 0.7353853219016884 2307 32-33 0.729900550249198 2307 34-35 0.7570359916760978 2307 36-37 0.6137321746032569 2307 38-39 0.6417286765879027 2307 40-41 0.7053744658918149 2307 42-43 0.8090795032381806 2307 44-45 0.8802771605397268 2307 46-47 0.769164782566591 2307 48-49 0.6533503141934602 2307 50-51 0.6828549292945354 2307 52-53 0.7479434509479503 2307 54-55 0.7367977322748942 2307 56-57 0.7190787913387595 2307 58-59 0.6949614305703591 2307 60-61 0.6708242010201602 2307 62-63 0.8628878849978534 2307 64-65 0.766786620944643 2307 66-67 0.9349689703723456 2307 68-69 0.9518234075412835 2307 70-71 0.9360511797048545 2307 72-73 0.6228470966386226 2307 74-75 0.8204543889476241 2307 76-77 0.8068127562107676 2307 78-79 0.8678702600190284 2307 80-81 1.024292400357023 2307 82-83 0.9549751630952521 2307 84-85 1.0395437047196907 2307 86-87 1.0817250079640814 2307 88-89 0.82772162336056 2307 90-91 0.7925803811612084 2307 92-93 1.181444987519889 2307 94-95 1.1345970869767648 2307 96-97 1.1730631050234948 2307 98-99 1.2437363147966742 2307 100-101 1.1024896742141301 2201 1 0.05226614207429492 2201 2 0.08105756340572157 2201 3 0.0449983363391695 2201 4 0.13720290068050645 2201 5 0.11689978553924618 2201 6 0.08137299776974771 2201 7 0.1012030896920777 2201 8 0.21778391018972343 2201 9 0.2432189499074937 2201 10-11 0.1982474049953069 2201 12-13 0.19348785674054625 2201 14-15 0.3999076677639195 2201 16-17 0.34699915706725903 2201 18-19 0.42470495136717545 2201 20-21 0.39154880323142294 2201 22-23 0.4383451015508868 2201 24-25 0.4752907690029957 2201 26-27 0.41016824017953724 2201 28-29 0.40750715815077143 2201 30-31 0.4987768827002341 2201 32-33 0.5538706046956605 2201 34-35 0.5586398936724706 2201 36-37 0.45633653032013655 2201 38-39 0.5172754098002414 2201 40-41 0.5780718343128655 2201 42-43 0.5170309551438095 2201 44-45 0.6067041115379084 2201 46-47 0.6815876500801963 2201 48-49 0.4693303504910986 2201 50-51 0.5180296116901815 2201 52-53 0.6040709464107508 2201 54-55 0.8328322150335126 2201 56-57 0.8733687187434853 2201 58-59 0.8922368389188122 2201 60-61 0.8531631302397571 2201 62-63 0.7505580301884152 2201 64-65 0.6994522289301202 2201 66-67 0.9254272280855886 2201 68-69 0.9325652405721314 2201 70-71 0.513208620721187 2201 72-73 0.5014405630632979 2201 74-75 0.5220696339566935 2201 76-77 0.3358123024902575 2201 78-79 0.4144650876052367 2201 80-81 0.5226204402844239 2201 82-83 0.4090042012077788 2201 84-85 0.4425291856634246 2201 86-87 0.6311011422653579 2201 88-89 0.5180256161010313 2201 90-91 0.5684900907800845 2201 92-93 0.6397480728193443 2201 94-95 0.4738053446900139 2201 96-97 0.6577318890525348 2201 98-99 0.5796437558130094 2201 100-101 0.41873059980396476 1101 1 0.12564272202480709 1101 2 0.11459932760019598 1101 3 0.12834365166050787 1101 4 0.11388818122621558 1101 5 0.15150906126986996 1101 6 0.14869560861706788 1101 7 0.20102670368257947 1101 8 0.181245255739114 1101 9 0.10848929699503174 1101 10-11 0.18599108519326535 1101 12-13 0.17819828274201654 1101 14-15 0.31912830910679446 1101 16-17 0.32970789444438253 1101 18-19 0.37208214992264743 1101 20-21 0.3253497127479079 1101 22-23 0.47073952073098013 1101 24-25 0.35383520824595394 1101 26-27 0.3213629001140035 1101 28-29 0.34861220396097536 1101 30-31 0.2810927223310813 1101 32-33 0.3913171037259531 1101 34-35 0.4280770467521151 1101 36-37 0.7018340893383908 1101 38-39 0.5392513510829318 1101 40-41 0.5755777695766895 1101 42-43 0.6350795908690827 1101 44-45 0.6335168748629982 1101 46-47 0.7129609530963137 1101 48-49 0.5758491092686029 1101 50-51 0.8186937760719104 1101 52-53 0.7855901231645745 1101 54-55 0.7164726216408752 1101 56-57 0.8099897145302819 1101 58-59 0.6281840568683918 1101 60-61 0.5809280436350619 1101 62-63 0.6431753143454415 1101 64-65 0.6363256824177128 1101 66-67 0.739829856320732 1101 68-69 0.8501917202083291 1101 70-71 0.8658899388513319 1101 72-73 0.2732672867976689 1101 74-75 0.33256585545071005 1101 76-77 0.35644821167236174 1101 78-79 0.628926650502315 1101 80-81 0.7344654713818706 1101 82-83 0.6327786946204732 1101 84-85 0.6963662083344602 1101 86-87 0.792865085955583 1101 88-89 0.6554988656161811 1101 90-91 0.7223358909539606 1101 92-93 0.7007040682717616 1101 94-95 0.807768011044594 1101 96-97 0.9598426694979274 1101 98-99 1.0300299659380663 1101 100-101 0.8868252129250784 2304 1 0.08226841768302506 2304 2 0.08453111758146292 2304 3 0.09029798524525035 2304 4 0.15689910691567377 2304 5 0.14410021790489935 2304 6 0.05209810423572492 2304 7 0.09640033620551947 2304 8 0.1499817418596976 2304 9 0.1312455907839265 2304 10-11 0.23518317593902793 2304 12-13 0.16201537535177124 2304 14-15 0.2990106309315692 2304 16-17 0.3466511108399004 2304 18-19 0.33796301352380453 2304 20-21 0.31365390384583947 2304 22-23 0.43390583592232446 2304 24-25 0.4048891240629757 2304 26-27 0.475684437637355 2304 28-29 0.5293202900385481 2304 30-31 0.3540390246982241 2304 32-33 0.3582816283944936 2304 34-35 0.39819464644383373 2304 36-37 0.4363184879938089 2304 38-39 0.44490475377475747 2304 40-41 0.49610948751009687 2304 42-43 0.5144979181814193 2304 44-45 0.6527959567315023 2304 46-47 0.6062528105106111 2304 48-49 0.6848199314540011 2304 50-51 0.5531026343487397 2304 52-53 0.3484422688645097 2304 54-55 0.3110440742058387 2304 56-57 0.635738074421127 2304 58-59 0.7262074104216865 2304 60-61 0.5937459705245303 2304 62-63 0.7998966814026147 2304 64-65 0.7428974599044054 2304 66-67 0.6564409305009846 2304 68-69 0.6644763861785847 2304 70-71 0.7679774432562141 2304 72-73 0.7234302578066476 2304 74-75 0.5352909206508869 2304 76-77 0.6686093100647845 2304 78-79 0.8337246614162304 2304 80-81 0.7454827903380625 2304 82-83 0.6155547034671329 2304 84-85 0.6014682643669786 2304 86-87 0.5719371440208256 2304 88-89 0.6026160878022111 2304 90-91 0.7114472849545237 2304 92-93 1.1126626399660537 2304 94-95 0.8905471199899075 2304 96-97 0.9737444536635671 2304 98-99 0.9347484338143737 2304 100-101 0.8567159952722534 2202 1 0.13196315909756606 2202 2 0.14042817093343984 2202 3 0.04988471772289671 2202 4 0.11671640997303001 2202 5 0.12405853924844479 2202 6 0.024403608421003753 2202 7 0.14316584152715706 2202 8 0.22889003231997407 2202 9 0.15114687431792362 2202 10-11 0.14305355055207514 2202 12-13 0.1375492342198612 2202 14-15 0.21855029621933397 2202 16-17 0.093622810042433 2202 18-19 0.24682641786725412 2202 20-21 0.20310979026875486 2202 22-23 0.20571684136034918 2202 24-25 0.1931144454046816 2202 26-27 0.1863014980274187 2202 28-29 0.285662905456725 2202 30-31 0.19073475401056328 2202 32-33 0.15480180881871775 2202 34-35 0.18489028411445219 2202 36-37 0.27779131713881355 2202 38-39 0.3448044977277718 2202 40-41 0.44335666173751065 2202 42-43 0.41495282770354436 2202 44-45 0.4705247815363478 2202 46-47 0.30882097820763477 2202 48-49 0.4030372066522716 2202 50-51 0.22483794372204358 2202 52-53 0.45643214428674383 2202 54-55 0.383796606724907 2202 56-57 0.5473311191807824 2202 58-59 0.4167653802275808 2202 60-61 0.3336810515266535 2202 62-63 0.5235345646253933 2202 64-65 0.4237230785318715 2202 66-67 0.5321643044560389 2202 68-69 0.6109669663256909 2202 70-71 0.5282142821299018 2202 72-73 0.4069809347640643 2202 74-75 0.4359225154185111 2202 76-77 0.48407971620258294 2202 78-79 0.5132595979909951 2202 80-81 0.45844056366409447 2202 82-83 0.48784224587434366 2202 84-85 0.314163575793053 2202 86-87 0.37097600561066457 2202 88-89 0.3244073902693927 2202 90-91 0.22808227413163706 2202 92-93 0.3619525082402397 2202 94-95 0.2548191272919169 2202 96-97 0.37328514533864876 2202 98-99 0.44652256262231305 2202 100-101 0.4625469749406186 1102 1 0.025166543164282018 1102 2 0.01675610294829255 1102 3 0.046688808155707306 1102 4 0.03596212421928868 1102 5 0.09088902346827155 1102 6 0.0543675478447625 1102 7 0.13197411629873557 1102 8 0.12346920763928182 1102 9 0.0831147736728397 1102 10-11 0.06389929103676195 1102 12-13 0.06642117170335382 1102 14-15 0.17375663901403726 1102 16-17 0.10554662193361253 1102 18-19 0.126983088901234 1102 20-21 0.038999126246636706 1102 22-23 0.10296249691538861 1102 24-25 0.12124083176898637 1102 26-27 0.17903535233164547 1102 28-29 0.0705064156750339 1102 30-31 -0.03576450458123048 1102 32-33 0.2772941832756146 1102 34-35 -0.028002623396822912 1102 36-37 -0.043962756290810034 1102 38-39 0.15254932211156103 1102 40-41 0.09321623436185433 1102 42-43 0.142485051772816 1102 44-45 0.1783861252545691 1102 46-47 0.22927832091085065 1102 48-49 0.13261913528029368 1102 50-51 0.02364047020645188 1102 52-53 0.17008182329755783 1102 54-55 -0.03236557437894305 1102 56-57 0.09676569832157611 1102 58-59 -0.024792837147437297 1102 60-61 -0.19537507281587096 1102 62-63 0.07764786975241833 1102 64-65 -0.12486289461172362 1102 66-67 0.22129883336405243 1102 68-69 0.20294715631261795 1102 70-71 0.14356497080232344 1102 72-73 0.1099683178779145 1102 74-75 0.02412713372706321 1102 76-77 -0.05934419149339121 1102 78-79 0.11292712949005335 1102 80-81 0.29058816172589985 1102 82-83 0.2644648423394038 1102 84-85 0.0847668697514834 1102 86-87 0.17921452673000715 1102 88-89 0.11077652697332496 1102 90-91 0.13454383224543776 1102 92-93 0.299719445404385 1102 94-95 -0.23944535951377688 1102 96-97 0.09943950670049517 1102 98-99 0.17310001863410918 1102 100-101 0.042945833569007164 2305 1 0.07110041784637389 2305 2 0.06531883642297487 2305 3 0.025477167715131088 2305 4 0.05361220907546027 2305 5 0.09413807755026227 2305 6 -0.08938602789358896 2305 7 -0.11324340878552164 2305 8 0.043046705608020375 2305 9 0.04621844244047679 2305 10-11 0.05719060493731121 2305 12-13 0.0018501881890031768 2305 14-15 0.0630157219903964 2305 16-17 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-0.29082908312610556 1213 72-73 -0.1873367745962966 1213 74-75 0.02220447867457409 1213 76-77 -0.09535974040721484 1213 78-79 -0.3656840316902006 1213 80-81 -0.5750405832712637 1213 82-83 -0.4127184400631343 1213 84-85 -0.47645336880120226 1213 86-87 -0.46046184496653453 1213 88-89 -0.3067937130464955 1213 90-91 -0.3093461255614116 1213 92-93 -0.22507747182689997 1213 94-95 -0.07227019995622896 1213 96-97 -0.3303854469215075 1213 98-99 -0.13701904811434318 1213 100-101 0.13491644321553764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 8.0 10 9.0 11 15.0 12 38.0 13 153.0 14 790.0 15 2328.0 16 4960.0 17 7549.0 18 7524.0 19 7313.0 20 7881.0 21 8884.0 22 10452.0 23 12525.0 24 14997.0 25 18323.0 26 23311.0 27 31304.0 28 46607.0 29 64738.0 30 59239.0 31 60003.0 32 79223.0 33 113055.0 34 171144.0 35 278603.0 36 430246.0 37 379659.0 38 46072.0 39 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.140346592477755 23.233057956548517 21.98228810499713 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28.99193732160516 22.780007694892223 20-21 25.273587335436886 25.065687458067607 29.200685120982573 20.460040085512933 22-23 23.68320361893113 29.641366087147304 25.88223635190035 20.79319394202121 24-25 23.720724167791047 24.49387005383245 28.568532766356153 23.216873012020354 26-27 22.653084369299712 24.75245976875047 26.681376347003606 25.91307951494621 28-29 24.79660470829893 27.087981447466465 26.043474021259527 22.071939822975082 30-31 22.42456938810768 25.27205047679714 28.5352518275369 23.76812830755827 32-33 22.868112091049866 27.18665837116302 25.98409298312741 23.961136554659703 34-35 23.158657866649428 26.83749998675122 28.406553165239863 21.597288981359494 36-37 22.651521013097213 27.653227456138584 28.69256785760846 21.002683673155744 38-39 22.616252757071404 26.09850945909995 27.40216304904275 23.883074734785893 40-41 25.33893032518869 25.423828516253078 24.929569478199394 24.30767168035884 42-43 23.190931898084013 27.33766798129908 27.390530618993665 22.08086950162324 44-45 22.71026610972323 25.19700937907731 27.276432113774174 24.816292397425286 46-47 25.093662334400037 25.091860498700424 25.471305898971604 24.343171267927936 48-49 22.869024025921888 27.163078902718453 27.27172273881041 22.696174332549244 50-51 24.986327243127054 24.88250968221969 26.43124087157996 23.699922203073296 52-53 22.708113343287813 24.93533783183994 24.64221503532869 27.714333789543556 54-55 25.147564894368607 24.91518695439944 27.343747101569488 22.59350104966247 56-57 22.86763505534508 24.98571586064192 27.067463539668037 25.079185544344966 58-59 22.514454443954758 25.09819375434455 27.314201728040466 25.073150073660223 60-61 24.506367700979194 25.085887979021738 27.392972250067636 23.01477206993143 62-63 22.65392003251844 24.965744613140025 27.457491125132126 24.922844229209414 64-65 25.1781145365577 25.544930443573765 27.071581042279757 22.20537397758877 66-67 22.99810350274245 29.519843785446685 24.795350030424522 22.68670268138634 68-69 22.617273100259 28.548884862607565 25.550545085221582 23.283296951911854 70-71 22.612277026194327 29.05199773663615 25.249148919535298 23.08657631763423 72-73 22.921649069244797 29.034023449094686 25.317866683628036 22.72646079803248 74-75 23.503365491264724 28.491294671904026 25.35338519629095 22.651954640540303 76-77 23.521532946725628 27.74204456854336 25.274120976303717 23.462301508427295 78-79 23.299154512754384 27.887458455693938 25.486805724488708 23.32658130706297 80-81 23.501140896399296 28.153735226709937 25.364468105910586 22.980655770980185 82-83 23.0188115293718 27.862728009604893 25.526370667655208 23.592089793368096 84-85 22.880005638850193 27.97887233065497 25.313429181420734 23.827692849074108 86-87 22.51043645476441 28.285681120458634 25.45864216900166 23.7452402557753 88-89 22.481855664748128 28.368925894575487 25.69764193871023 23.451576501966155 90-91 22.947728016601097 27.897313850545086 25.239044906969994 23.915913225883823 92-93 22.648444593565294 27.673136992059316 24.879400279760606 24.799018134614784 94-95 22.89070127623306 28.111368004384502 24.512812684520572 24.485118034861866 96-97 22.733057529265533 28.833327591096175 24.263081037101035 24.170533842537257 98-99 22.855794132700325 27.73003201535592 24.805738432501244 24.608435419442507 100-101 23.490794102416373 27.86291303571211 24.368557104020784 24.27773575785073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3631.0 1 2144.0 2 791.0 3 2186.0 4 2679.0 5 1578.0 6 1415.5 7 1661.0 8 1752.0 9 1674.0 10 1483.5 11 1335.0 12 1333.5 13 1330.5 14 1269.0 15 1251.5 16 1249.5 17 1305.5 18 1362.5 19 1399.0 20 1455.0 21 1497.0 22 1604.5 23 1719.0 24 1820.0 25 2049.0 26 2331.0 27 2654.0 28 3042.5 29 3522.0 30 4300.5 31 5366.0 32 6676.0 33 8438.0 34 11432.5 35 16725.5 36 23603.5 37 32638.0 38 42110.5 39 48246.0 40 55826.5 41 60667.5 42 65478.0 43 76240.5 44 81544.0 45 84931.0 46 94635.0 47 100108.5 48 96897.5 49 93444.0 50 90460.5 51 88212.0 52 85678.0 53 82344.0 54 76997.5 55 67857.5 56 58710.0 57 51934.0 58 47521.0 59 41097.0 60 31979.0 61 23741.5 62 16733.0 63 11620.0 64 8794.0 65 7349.0 66 6790.5 67 6252.0 68 5337.5 69 4060.5 70 2815.5 71 2034.5 72 1688.0 73 1426.0 74 982.0 75 594.5 76 416.0 77 327.5 78 263.5 79 208.0 80 169.5 81 138.0 82 109.5 83 80.5 84 58.0 85 42.0 86 31.0 87 22.5 88 17.5 89 12.0 90 11.0 91 11.0 92 9.0 93 6.0 94 2.0 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1847410075221281 2 0.0 3 0.0 4 0.02405978698079351 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 7.949268826253361E-5 48-49 0.0035241758463056567 50-51 1.0599025101671148E-4 52-53 0.0023052879596134747 54-55 0.00895617621091212 56-57 0.013672742381155782 58-59 0.013911220445943383 60-61 0.0032062050932555224 62-63 0.0019343220810549846 64-65 0.009989581158325057 66-67 0.017488391417757394 68-69 0.02501369923994391 70-71 0.020853581887537984 72-73 0.01727641091572397 74-75 0.020747591636521272 76-77 0.015845542526998366 78-79 0.006624390688544468 80-81 0.0038421465993557914 82-83 6.09443943346091E-4 84-85 0.0029942245912220997 86-87 0.01642848890759028 88-89 0.01531559127191481 90-91 0.018203825612120198 92-93 0.018707279304449577 94-95 0.016958440162673838 96-97 0.01913124030851642 98-99 0.02795492870565765 100-101 0.015633562024964943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1886966.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.87058413340324 #Duplication Level Percentage of deduplicated Percentage of total 1 82.26188521607968 38.556626119926236 2 8.342921844050196 7.820752404199248 3 2.8984499590029724 4.07556127979724 4 1.5002933080281933 2.812784948748692 5 0.8211507500980115 1.9243907659338015 6 0.6579988824786069 1.850447518853932 7 0.551629510033907 1.8098638152358575 8 0.34858928418910606 1.3070866698070647 9 0.30523471793997287 1.2875876574876994 >10 2.0447960786010633 18.16221634325207 >50 0.1672188719357142 5.365891749879638 >100 0.09448962364418348 7.896392086636691 >500 0.00376808010933057 1.1798522506822697 >1k 0.0013495216653832776 1.3138347528267083 >5k 0.0 0.0 >10k+ 2.2435214369398212E-4 4.636711636732919 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT 72521 3.843259496991467 TruSeq Adapter, Index 27 (97% over 39bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15667 0.8302746313394094 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4459 0.23630526464175827 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 4083 0.2163790974506165 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 3998 0.21187451178240627 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT 2277 0.12066990078252603 TruSeq Adapter, Index 27 (97% over 39bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003073717279484633 0.0 0.0 2 0.0036036685345681905 0.0 0.0 3 0.004027629538635036 0.0 0.0 4 0.0041336197896517475 0.0 0.0 5 0.005246517425327219 0.0 0.0 6 0.005617483303885709 0.0 0.0 7 0.007154341943628025 0.0 0.0 8 0.008426224955828563 0.0 0.0 9 0.010016078721079236 0.0 0.0 10-11 0.018654284178941223 0.0 0.0 12-13 0.0350827730865315 0.0 0.0 14-15 0.05689026723321989 0.0 0.0 16-17 0.09101912806060099 0.0 0.0 18-19 0.1310039502566554 0.0 0.0 20-21 0.14433222432200687 0.0 0.0 22-23 0.15302342490537718 0.0 0.0 24-25 0.16131716204743488 0.0 0.0 26-27 0.1724461384041896 0.0 0.0 28-29 0.18352211963543594 0.0 0.0 30-31 0.23990893317632644 0.0 0.0 32-33 0.25172684616468977 0.0 0.0 34-35 0.26065652481284773 0.0 0.0 36-37 0.2695862034610057 0.0 0.0 38-39 0.27931080899178895 0.0 0.0 40-41 0.2883199803282094 0.0 0.0 42-43 0.29764712241768 0.0 0.0 44-45 0.3062058351872795 0.0 0.0 46-47 0.31603643096907946 0.0 0.0 48-49 0.3270064219493091 0.0 0.0 50-51 0.33845336905911394 0.0 0.0 52-53 0.3513841796831527 0.0 0.0 54-55 0.364977429376046 0.0 0.0 56-57 0.3794450986398271 0.0 0.0 58-59 0.3959000851101716 0.0 0.0 60-61 0.4134149740906831 0.0 0.0 62-63 0.4335796193466125 0.0 0.0 64-65 0.4570299623840599 0.0 0.0 66-67 0.482070159186758 0.0 0.0 68-69 0.5076138096817855 0.0 0.0 70-71 0.5369466116506604 0.0 0.0 72-73 0.5715789261703709 0.0 0.0 74-75 0.6108218166093082 0.0 0.0 76-77 0.6550992439715395 0.0 0.0 78-79 0.7041462326295227 0.0 0.0 80-81 0.7569028800730908 0.0 0.0 82-83 0.8148265522537237 0.0 0.0 84-85 0.8798515712524762 0.0 0.0 86-87 0.953408805458074 0.0 0.0 88-89 1.036717142757209 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGAA 10760 0.0 71.1235 2 GATCGGA 10780 0.0 71.01823 1 CGGAAGA 10845 0.0 70.566055 4 TCGGAAG 10955 0.0 69.85749 3 GAAGAGC 11195 0.0 68.51327 6 GGAAGAG 13400 0.0 57.381027 5 GAGCACA 14790 0.0 51.76344 9 AAGAGCA 15230 0.0 50.33034 7 AGAGCAC 15570 0.0 49.322792 8 TATGCCG 8910 0.0 42.567783 48-49 GTATGCC 9010 0.0 42.33144 46-47 TACTCGA 8960 0.0 41.953594 36-37 GATCTCG 8765 0.0 41.91164 40-41 CTCGATC 8770 0.0 41.752357 38-39 CTCGTAT 8835 0.0 41.637737 44-45 CTGCTTG 9225 0.0 41.63411 58-59 GCCGTCT 9185 0.0 41.60578 50-51 TCGATCT 8845 0.0 41.55941 38-39 TCGTATG 9230 0.0 41.528297 44-45 CACATTA 9245 0.0 41.199665 30-31 >>END_MODULE