FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_40825-1.341000000356f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_40825-1.341000000356f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886966
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT725213.843259496991467TruSeq Adapter, Index 27 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156670.8302746313394094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44590.23630526464175827No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA40830.2163790974506165No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC39980.21187451178240627No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATATCGTAT22770.12066990078252603TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA107600.071.12352
GATCGGA107800.071.018231
CGGAAGA108450.070.5660554
TCGGAAG109550.069.857493
GAAGAGC111950.068.513276
GGAAGAG134000.057.3810275
GAGCACA147900.051.763449
AAGAGCA152300.050.330347
AGAGCAC155700.049.3227928
TATGCCG89100.042.56778348-49
GTATGCC90100.042.3314446-47
TACTCGA89600.041.95359436-37
GATCTCG87650.041.9116440-41
CTCGATC87700.041.75235738-39
CTCGTAT88350.041.63773744-45
CTGCTTG92250.041.6341158-59
GCCGTCT91850.041.6057850-51
TCGATCT88450.041.5594138-39
TCGTATG92300.041.52829744-45
CACATTA92450.041.19966530-31
Produced by FastQC (version 0.11.2)