##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_40255-1.341000000357f8.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7809676 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.683211057667435 33.0 33.0 33.0 33.0 33.0 2 32.71327799002161 33.0 33.0 33.0 33.0 33.0 3 32.74424316706609 33.0 33.0 33.0 33.0 33.0 4 36.672938288348966 37.0 37.0 37.0 37.0 37.0 5 36.66471489982427 37.0 37.0 37.0 37.0 37.0 6 36.539156553997884 37.0 37.0 37.0 37.0 37.0 7 36.459257336668 37.0 37.0 37.0 37.0 37.0 8 36.3920773409806 37.0 37.0 37.0 37.0 37.0 9 36.42229293507182 37.0 37.0 37.0 37.0 37.0 10-11 36.37329660026869 37.0 37.0 37.0 37.0 37.0 12-13 36.353495842849306 37.0 37.0 37.0 37.0 37.0 14-15 38.19559185297828 40.0 37.0 40.0 37.0 40.0 16-17 38.171364151342516 40.0 37.0 40.0 37.0 40.0 18-19 38.15449027590901 40.0 37.0 40.0 37.0 40.0 20-21 38.09012518573114 40.0 37.0 40.0 37.0 40.0 22-23 37.94334476872024 40.0 37.0 40.0 33.0 40.0 24-25 37.94694350956429 40.0 37.0 40.0 33.0 40.0 26-27 37.84641942892381 40.0 37.0 40.0 33.0 40.0 28-29 37.682217674587264 40.0 37.0 40.0 33.0 40.0 30-31 37.64081819783561 40.0 37.0 40.0 33.0 40.0 32-33 37.50464033335058 40.0 37.0 40.0 33.0 40.0 34-35 37.370580418445016 40.0 37.0 40.0 33.0 40.0 36-37 37.21869812524873 40.0 37.0 40.0 33.0 40.0 38-39 37.17534504632458 40.0 37.0 40.0 33.0 40.0 40-41 37.11955291102986 40.0 37.0 40.0 33.0 40.0 42-43 36.90715818428319 37.0 37.0 40.0 33.0 40.0 44-45 36.781311094083804 37.0 37.0 40.0 33.0 40.0 46-47 36.75950545195473 37.0 37.0 40.0 33.0 40.0 48-49 36.50590722329582 37.0 37.0 40.0 33.0 40.0 50-51 36.362137494564436 37.0 37.0 40.0 33.0 40.0 52-53 36.22574406415836 37.0 37.0 40.0 33.0 40.0 54-55 36.00497210127539 37.0 37.0 40.0 33.0 40.0 56-57 35.8293461214012 37.0 37.0 40.0 30.0 40.0 58-59 35.597241101935595 37.0 33.0 40.0 27.0 40.0 60-61 35.296681450037106 37.0 33.0 40.0 27.0 40.0 62-63 35.03751640913144 37.0 33.0 38.5 27.0 40.0 64-65 34.78498704683779 37.0 33.0 37.0 27.0 40.0 66-67 34.34659498038074 37.0 33.0 37.0 27.0 40.0 68-69 34.091821222801045 37.0 33.0 37.0 27.0 40.0 70-71 33.72708938245326 37.0 33.0 37.0 27.0 38.5 72-73 33.22547702363069 37.0 33.0 37.0 27.0 37.0 74-75 32.727675770416084 37.0 33.0 37.0 22.0 37.0 76-77 30.156982440756824 33.0 30.0 35.0 22.0 37.0 78-79 32.42794963581076 33.0 33.0 37.0 24.5 37.0 80-81 32.813528051611875 37.0 33.0 37.0 27.0 37.0 82-83 32.70944498849889 37.0 33.0 37.0 24.5 37.0 84-85 32.52035359725551 37.0 33.0 37.0 22.0 37.0 86-87 32.396707878790366 33.0 33.0 37.0 22.0 37.0 88-89 32.263956212780144 33.0 33.0 37.0 22.0 37.0 90-91 32.12556244330751 33.0 33.0 37.0 22.0 37.0 92-93 31.98561771320603 33.0 33.0 37.0 22.0 37.0 94-95 31.858074842029296 33.0 33.0 37.0 22.0 37.0 96-97 31.68039608813477 33.0 33.0 37.0 22.0 37.0 98-99 31.497146040373508 33.0 33.0 37.0 15.0 37.0 100-101 29.798844151793237 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1340564455572988 2306 2 0.09880796937802216 2306 3 0.12680730623969794 2306 4 0.1320649862214225 2306 5 0.19210642300294012 2306 6 0.1793844638679971 2306 7 0.2796387360263495 2306 8 0.23781250447642321 2306 9 0.17503434024335007 2306 10-11 0.24803970382282614 2306 12-13 0.23323731667242953 2306 14-15 0.4304394506566851 2306 16-17 0.4364091813355415 2306 18-19 0.3934718814064695 2306 20-21 0.45975524480022756 2306 22-23 0.476030604512907 2306 24-25 0.41228444526898045 2306 26-27 0.4698355103401255 2306 28-29 0.4564686886869822 2306 30-31 0.4597853518285433 2306 32-33 0.6390583773486682 2306 34-35 0.6733851250519294 2306 36-37 0.6120937625967144 2306 38-39 0.6045290270345376 2306 40-41 0.5794867093862521 2306 42-43 0.6013607417996312 2306 44-45 0.6264182710393342 2306 46-47 0.596649308245226 2306 48-49 0.6935228522285968 2306 50-51 0.6033568790359638 2306 52-53 0.6430430405625103 2306 54-55 0.631209647813769 2306 56-57 0.5953218455216316 2306 58-59 0.6161984593654708 2306 60-61 0.6226069855877512 2306 62-63 0.5861575916730359 2306 64-65 0.5748661177091563 2306 66-67 0.5835710055389711 2306 68-69 0.7076593435949192 2306 70-71 0.6943575535984365 2306 72-73 0.44525762777966094 2306 74-75 0.5609830836882779 2306 76-77 0.6259467643797869 2306 78-79 0.7497327530071942 2306 80-81 0.6626115319882473 2306 82-83 0.7218399580028532 2306 84-85 0.6536252252820347 2306 86-87 0.7099318515799737 2306 88-89 0.622359880661449 2306 90-91 0.6650644091763738 2306 92-93 0.8023396864926866 2306 94-95 0.7027272223896581 2306 96-97 0.7463043174059543 2306 98-99 0.9459001064803658 2306 100-101 0.7800982548253756 2307 1 0.1192793858999579 2307 2 0.11327449550728375 2307 3 0.08670298515866648 2307 4 0.14637673516653393 2307 5 0.1440048144742363 2307 6 0.17046624168363422 2307 7 0.20192121620728187 2307 8 0.2295131462387232 2307 9 0.21189921612471352 2307 10-11 0.22816003276735586 2307 12-13 0.22176326017505232 2307 14-15 0.43039575565180144 2307 16-17 0.4265903769497754 2307 18-19 0.40823512791700267 2307 20-21 0.46178140613093177 2307 22-23 0.49363047688642325 2307 24-25 0.495748251642091 2307 26-27 0.5473742985305918 2307 28-29 0.50418748071435 2307 30-31 0.49870399784921204 2307 32-33 0.5982109439397547 2307 34-35 0.528336557678081 2307 36-37 0.5336386906784796 2307 38-39 0.5587922642080869 2307 40-41 0.4857660994788233 2307 42-43 0.5647344661730145 2307 44-45 0.6011725071817509 2307 46-47 0.596048646179355 2307 48-49 0.5704074637745791 2307 50-51 0.5317118983103697 2307 52-53 0.4937595896845366 2307 54-55 0.5050852664067023 2307 56-57 0.532111801692345 2307 58-59 0.5171299935067069 2307 60-61 0.628723446991053 2307 62-63 0.6674107175531887 2307 64-65 0.5301327138903034 2307 66-67 0.5796195538192137 2307 68-69 0.6635638448833205 2307 70-71 0.6170503366943336 2307 72-73 0.48141315486032 2307 74-75 0.5346927661726468 2307 76-77 0.5874865224979473 2307 78-79 0.6200577284425179 2307 80-81 0.5890065706416294 2307 82-83 0.5839971497950032 2307 84-85 0.627884898314008 2307 86-87 0.5725077390851467 2307 88-89 0.4714222442566154 2307 90-91 0.4649192207640098 2307 92-93 0.622991274658375 2307 94-95 0.39527976113064156 2307 96-97 0.6254214132348501 2307 98-99 0.6783072630752258 2307 100-101 0.5303888204880778 2201 1 0.1286890602407169 2201 2 0.11914478771695514 2201 3 0.0896202458263815 2201 4 0.15212712518163585 2201 5 0.12115943487089709 2201 6 0.1114690978040116 2201 7 0.16132013233076492 2201 8 0.2005021744426898 2201 9 0.20806196535740185 2201 10-11 0.22610219272246468 2201 12-13 0.22053558664705974 2201 14-15 0.35617642406293726 2201 16-17 0.3953597716688435 2201 18-19 0.40238663754778514 2201 20-21 0.43528535194693063 2201 22-23 0.47540661178320676 2201 24-25 0.4181305918659106 2201 26-27 0.4707026483595982 2201 28-29 0.4549201573286865 2201 30-31 0.5043239573627929 2201 32-33 0.48395843867044874 2201 34-35 0.44996534701633095 2201 36-37 0.45162831680783455 2201 38-39 0.5178564467651228 2201 40-41 0.45444592430936126 2201 42-43 0.43109343355539664 2201 44-45 0.47862157134370165 2201 46-47 0.4539747726646013 2201 48-49 0.4733677255996227 2201 50-51 0.5473981718874725 2201 52-53 0.51794696373927 2201 54-55 0.6126413086321847 2201 56-57 0.6144227714112489 2201 58-59 0.4711399862018979 2201 60-61 0.48662807289763776 2201 62-63 0.5486669538705584 2201 64-65 0.5428112055581167 2201 66-67 0.6451050681214525 2201 68-69 0.6314369857611055 2201 70-71 0.4301241631171209 2201 72-73 0.4419476852494668 2201 74-75 0.45828705088755584 2201 76-77 0.3248829419539412 2201 78-79 0.49526955908835646 2201 80-81 0.5789435704578594 2201 82-83 0.5379223927903851 2201 84-85 0.5696052648957064 2201 86-87 0.6256712337029455 2201 88-89 0.52531098700711 2201 90-91 0.6486739321417261 2201 92-93 0.6035188538279925 2201 94-95 0.6335394541892647 2201 96-97 0.5915321091860655 2201 98-99 0.6008702018174432 2201 100-101 0.6551906576142166 1101 1 0.104555613393984 1101 2 0.0896582679124478 1101 3 0.08082938658131411 1101 4 0.13816569718696314 1101 5 0.12867780223545822 1101 6 0.15211658473690193 1101 7 0.1348184487006634 1101 8 0.1340453108523505 1101 9 0.18277779205612887 1101 10-11 0.2150376592056702 1101 12-13 0.1588942146126655 1101 14-15 0.33188314210328684 1101 16-17 0.2594274565497088 1101 18-19 0.3203897853135871 1101 20-21 0.34419329886814154 1101 22-23 0.30787285822002275 1101 24-25 0.32878456287969726 1101 26-27 0.3170721027972334 1101 28-29 0.33244918276628255 1101 30-31 0.39167460220860306 1101 32-33 0.39850724811115157 1101 34-35 0.4087201156224367 1101 36-37 0.4610693113118387 1101 38-39 0.47572935158298435 1101 40-41 0.48527812478764076 1101 42-43 0.4611586989601264 1101 44-45 0.4222745485155528 1101 46-47 0.4777409801681358 1101 48-49 0.4980557376782926 1101 50-51 0.4802392140325651 1101 52-53 0.5471162601409318 1101 54-55 0.5925434644991228 1101 56-57 0.526681498473188 1101 58-59 0.5007614410972323 1101 60-61 0.4769187914503803 1101 62-63 0.5524188690492835 1101 64-65 0.4989457146138747 1101 66-67 0.5678553403305102 1101 68-69 0.5381244408277226 1101 70-71 0.433973447457376 1101 72-73 0.38410378248045873 1101 74-75 0.4340237065267232 1101 76-77 0.21329356490787532 1101 78-79 0.5158823874933915 1101 80-81 0.4988557439059491 1101 82-83 0.41105318195557317 1101 84-85 0.5502969529660788 1101 86-87 0.5159646149748198 1101 88-89 0.41993495709254347 1101 90-91 0.5681179707813797 1101 92-93 0.6473572138232981 1101 94-95 0.5742175264094094 1101 96-97 0.633253463545767 1101 98-99 0.6521572666877589 1101 100-101 0.5080078504317846 2304 1 0.005882383256334833 2304 2 0.04330435128336774 2304 3 0.0744188080976258 2304 4 0.1099923005083383 2304 5 0.08831109562598272 2304 6 0.1291249858036494 2304 7 0.024571699976654315 2304 8 0.11083474356293266 2304 9 0.07606322889499495 2304 10-11 0.07547641718011988 2304 12-13 0.12393589824183948 2304 14-15 0.2320307338894665 2304 16-17 0.23279412248840003 2304 18-19 0.1894016304502344 2304 20-21 0.23222801994699438 2304 22-23 0.2755362642058472 2304 24-25 0.26126184380117223 2304 26-27 0.2874517024371883 2304 28-29 0.3085739162647769 2304 30-31 0.2842343205836997 2304 32-33 0.2632428023656459 2304 34-35 0.2966475495301282 2304 36-37 0.36055033381455104 2304 38-39 0.38288178606935475 2304 40-41 0.35495477375575746 2304 42-43 0.3782480780402011 2304 44-45 0.3976973644585584 2304 46-47 0.4207393087428173 2304 48-49 0.5265955768024568 2304 50-51 0.4368747961027353 2304 52-53 0.3460807113920268 2304 54-55 0.3061524838402434 2304 56-57 0.35049738040080314 2304 58-59 0.41638701200844963 2304 60-61 0.35397457201751337 2304 62-63 0.3358563008423445 2304 64-65 0.32423106723414463 2304 66-67 0.43171511270228535 2304 68-69 0.40566493212293153 2304 70-71 0.4554000189744727 2304 72-73 0.2709628956193839 2304 74-75 0.37520439112193316 2304 76-77 0.29869761867334077 2304 78-79 0.533290162570097 2304 80-81 0.5625281429734201 2304 82-83 0.5320865676988475 2304 84-85 0.5678827379128393 2304 86-87 0.49882059842508397 2304 88-89 0.42737151165213305 2304 90-91 0.4885422625114657 2304 92-93 0.6157267744493709 2304 94-95 0.5197019121635442 2304 96-97 0.5181154712413054 2304 98-99 0.6689675474246997 2304 100-101 0.5637849258938701 2202 1 0.1140018107817582 2202 2 0.09127636435697895 2202 3 0.09109073465847217 2202 4 0.10295116259868564 2202 5 0.10902907363858816 2202 6 0.07998460474554747 2202 7 0.1528587058824371 2202 8 0.12041321792815296 2202 9 0.14886908620459138 2202 10-11 0.13137374042702987 2202 12-13 0.10052747792060757 2202 14-15 0.14136952646965995 2202 16-17 0.060110759219803356 2202 18-19 0.1462290202825116 2202 20-21 0.18464465569341115 2202 22-23 0.22827465854705764 2202 24-25 0.16737747039891815 2202 26-27 0.12681377370482494 2202 28-29 0.17471170718080486 2202 30-31 0.14495319608109725 2202 32-33 0.1377491009144336 2202 34-35 0.1960652980781461 2202 36-37 0.22257022302290608 2202 38-39 0.22028921920410482 2202 40-41 0.2738592428861324 2202 42-43 0.2592158694024036 2202 44-45 0.2517174143531875 2202 46-47 0.29951904807494856 2202 48-49 0.2973533461933684 2202 50-51 0.1556395116890812 2202 52-53 0.26208248633433584 2202 54-55 0.23621967751640938 2202 56-57 0.2638394207716672 2202 58-59 0.21997447586777952 2202 60-61 0.17730536785841622 2202 62-63 0.24504851515703763 2202 64-65 0.16341262379986432 2202 66-67 0.286907999288303 2202 68-69 0.3311693771877984 2202 70-71 0.22294045977753996 2202 72-73 0.2526763620863335 2202 74-75 0.3041784127506091 2202 76-77 0.23109380411962732 2202 78-79 0.35918074368471764 2202 80-81 0.34145550404922176 2202 82-83 0.30494943728576374 2202 84-85 0.3902610674825979 2202 86-87 0.3443612301893353 2202 88-89 0.35957782384802783 2202 90-91 0.32811722905875484 2202 92-93 0.46487429607996233 2202 94-95 0.20045998313367264 2202 96-97 0.33355781789171957 2202 98-99 0.43551691794416314 2202 100-101 0.49322080358803433 1102 1 0.06606691299559486 1102 2 0.037114235908894955 1102 3 0.05189740591364256 1102 4 0.07637453165681762 1102 5 0.07717504131620245 1102 6 0.07736042072815508 1102 7 0.07101184749722478 1102 8 0.09852002856870712 1102 9 0.10438561179086037 1102 10-11 0.04537580989690326 1102 12-13 0.08281703152869113 1102 14-15 0.14942199664612588 1102 16-17 0.09583538384354284 1102 18-19 0.06821772850540953 1102 20-21 0.0034382505491095117 1102 22-23 0.13065787212486413 1102 24-25 0.1190823671174357 1102 26-27 0.0997825683441178 1102 28-29 0.148897940057779 1102 30-31 0.13791705125365894 1102 32-33 0.21490818170520498 1102 34-35 0.13004907189825587 1102 36-37 0.09706599018897322 1102 38-39 0.10326023354396341 1102 40-41 0.13932255895841195 1102 42-43 0.17626208306166546 1102 44-45 0.12549967016818897 1102 46-47 0.11850316371207015 1102 48-49 0.10627763963440628 1102 50-51 0.01828140267139844 1102 52-53 0.013794936786688083 1102 54-55 0.019276557554256613 1102 56-57 0.14543127734018668 1102 58-59 0.11870016307820919 1102 60-61 -0.09719107450173681 1102 62-63 0.06897571615261455 1102 64-65 0.03359755102027151 1102 66-67 0.11236917794930434 1102 68-69 0.1144054790560105 1102 70-71 0.04622097253680835 1102 72-73 0.03907644454441339 1102 74-75 0.00667633547380575 1102 76-77 0.031530433145629644 1102 78-79 0.16032045020316588 1102 80-81 0.2687469228809718 1102 82-83 0.2110692804381884 1102 84-85 0.23028535969984176 1102 86-87 0.26344163483315697 1102 88-89 0.26483686838930964 1102 90-91 0.17457906434599835 1102 92-93 0.18732031114775793 1102 94-95 -0.03350337661521863 1102 96-97 0.23267898948850174 1102 98-99 0.25521639116617933 1102 100-101 0.22032118469289586 2305 1 0.031329336679206676 2305 2 0.015260811158363197 2305 3 0.03172223048599676 2305 4 0.024511268317546353 2305 5 0.04323302906310289 2305 6 0.009379527464702164 2305 7 -0.02085842119031156 2305 8 0.039095805993582644 2305 9 0.010187003530162997 2305 10-11 0.030691336927041846 2305 12-13 0.0315462833521849 2305 14-15 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-0.2579458640985095 1213 54-55 -0.41134946378697634 1213 56-57 -0.3678528181677052 1213 58-59 -0.27153722351769005 1213 60-61 -0.3640766945295084 1213 62-63 -0.3194390085783212 1213 64-65 -0.2801255611313209 1213 66-67 -0.22163578596116906 1213 68-69 -0.3252088573731484 1213 70-71 -0.34296420847431364 1213 72-73 -0.3018219003064857 1213 74-75 -0.2487067411261492 1213 76-77 -0.21308021492899698 1213 78-79 -0.4262135979196131 1213 80-81 -0.49707855135433476 1213 82-83 -0.44676719226543327 1213 84-85 -0.44420672314470266 1213 86-87 -0.5281952773993766 1213 88-89 -0.4461014080221233 1213 90-91 -0.3449387345259822 1213 92-93 -0.43319500833033686 1213 94-95 -0.35926986279157447 1213 96-97 -0.38696687724920054 1213 98-99 -0.25694839119528723 1213 100-101 -0.016122959788148705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 2.0 9 35.0 10 42.0 11 40.0 12 86.0 13 242.0 14 999.0 15 3067.0 16 6772.0 17 11497.0 18 13881.0 19 15937.0 20 19834.0 21 24251.0 22 30022.0 23 37306.0 24 46009.0 25 57443.0 26 73286.0 27 98107.0 28 142140.0 29 194027.0 30 184967.0 31 191546.0 32 254376.0 33 359594.0 34 552343.0 35 941760.0 36 1714587.0 37 2298885.0 38 536340.0 39 252.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.26840566828795 27.324397818815537 20.316732061831015 15.0904644510655 2 31.61423085925716 25.645378886396824 28.32076516362523 14.419625090720793 3 34.916659794849366 20.008282033723294 27.085169730472813 17.98988844095453 4 30.86622830189815 19.703475551487752 28.341589975886478 21.088706170727615 5 31.64432173626665 18.31260349340997 31.286816508136827 18.75625826218655 6 29.113602151997085 18.83907859941949 33.256514098664276 18.79080514991915 7 31.862102858044306 21.996853646681373 25.862263684178448 20.278779811095877 8 29.86396618758576 28.343262895925513 22.194659547976126 19.5981113685126 9 29.389375436317717 25.541392498229122 24.773857455802265 20.2953746096509 10-11 24.457282222719613 28.699282787147634 28.97298172164889 17.870453268483864 12-13 22.66181081007714 27.952292771172583 28.6138054894979 20.77209092925238 14-15 22.70102498490334 28.527976064564008 28.5434952743238 20.227503676208848 16-17 23.627401444054787 27.550041768698215 28.96859613638261 19.853960650864387 18-19 21.368933871264314 27.363484733553605 30.782979985341264 20.48460140984082 20-21 22.69124224871813 27.82380472634204 30.440232091574604 19.044720933365227 22-23 21.280485899799174 30.568726538719403 28.804773719165816 19.34601384231561 24-25 21.366424164075436 26.923760985731032 30.679236885115337 21.030577965078194 26-27 21.116887563581383 27.164827324462625 29.179667632818568 22.538617479137418 28-29 22.323506122405078 29.36533474628141 28.55222803097081 19.758931100342704 30-31 20.17455653730065 28.793268760445372 29.96457855613984 21.067596146114127 32-33 20.056978035964615 30.16587371870485 28.737261315322172 21.039886930008365 34-35 22.02821218191382 29.928392675957365 28.855684922140174 19.18771021998864 36-37 21.670758172298058 30.51522239847082 29.070456956216876 18.743562473014244 38-39 20.341746571816806 28.674499428657473 31.36296563391362 19.620788365612093 40-41 20.590329227486517 28.06389151099226 28.161578021930744 23.184201239590475 42-43 20.855154554427095 29.65827903744022 29.813202237839313 19.673364170293365 44-45 20.274067707802477 28.172442749225446 30.014126066177393 21.539363476794684 46-47 21.95121998065732 28.087859760581523 28.661340878164808 21.299579380596345 48-49 20.827846105137734 29.68148413022858 29.453740017267606 20.036929747366084 50-51 22.042233903888555 28.35595495765604 28.885330481485553 20.716480656969846 52-53 20.533413509433924 28.18526492686577 27.851580566993228 23.42974099670708 54-55 22.367913453069992 27.99362397661399 29.48487583305934 20.153586737256678 56-57 20.897881634335928 27.628385686251573 29.474544733777496 21.999187945635004 58-59 20.66549888884805 27.8465640861193 29.772206486648056 21.71573053838459 60-61 21.797580142408666 27.9706390731765 29.563233612609068 20.668547171805766 62-63 20.15790646682536 28.12843952231748 29.600115705282665 22.1135383055745 64-65 21.992214890186258 28.24082424608441 29.56543776467859 20.201523099050743 66-67 21.086192300040207 30.723875264485727 27.910905472737486 20.279026962736577 68-69 20.9432413596171 30.473135853931577 28.081613451672315 20.502009334779004 70-71 20.65757264770737 31.17016430222942 27.971568607486514 20.2006944425767 72-73 20.793760769741564 31.364529057731254 27.750640821634832 20.091069350892347 74-75 21.597172219844307 30.646332551311083 27.438914277502885 20.31758095134172 76-77 21.651330665762863 29.663903153476056 27.73620366630964 20.94856251445144 78-79 21.292277640894735 29.636616182926222 28.297058295869952 20.774047880309087 80-81 20.823286439755677 30.13295288683712 28.162288098073347 20.881472575333852 82-83 20.136536514362234 30.114336366019785 28.35952908155409 21.38959803806389 84-85 20.398558140818356 29.862409253959328 28.477378290711425 21.261654314510896 86-87 20.222244320835927 30.10420720109866 28.910523474394704 20.76302500367071 88-89 20.24452510132037 30.47643386102379 28.800073406963556 20.47896763069228 90-91 20.790159616789076 29.58674706471663 28.545685595299574 21.077407723194717 92-93 20.41684617871152 30.029171881267448 28.038285116526158 21.515696823494878 94-95 20.226796702291498 30.402936219999038 27.68504241752383 21.685224660185632 96-97 20.500119907109614 30.76863178783902 27.398009317855017 21.333238987196353 98-99 20.66449057560531 29.99777327337587 27.72129272591749 21.616443425101327 100-101 20.902318469184088 30.15962327677234 27.309770202160884 21.628288051882688 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3602.0 1 2251.0 2 1070.0 3 1705.5 4 1794.5 5 1298.5 6 1264.5 7 1439.5 8 1507.0 9 1470.0 10 1405.0 11 1374.0 12 1358.0 13 1403.0 14 1477.5 15 1480.0 16 1628.5 17 1812.5 18 2037.0 19 2422.0 20 2972.0 21 3800.0 22 5017.0 23 6835.0 24 9320.5 25 12458.0 26 16695.0 27 21983.5 28 28280.0 29 35738.5 30 44118.0 31 54449.0 32 70260.0 33 93627.5 34 125742.5 35 177896.0 36 249581.5 37 337664.0 38 423249.5 39 465340.0 40 492434.0 41 488085.0 42 460527.0 43 470167.5 44 467178.5 45 451873.0 46 462637.5 47 430710.0 48 342152.5 49 269540.0 50 224548.0 51 197896.0 52 177984.5 53 152306.5 54 120104.5 55 88150.0 56 65996.5 57 54421.5 58 46626.0 59 36336.5 60 26286.0 61 19434.5 62 14029.5 63 9776.0 64 6927.0 65 4841.0 66 3449.0 67 2540.5 68 1831.5 69 1251.0 70 800.0 71 533.0 72 357.0 73 249.5 74 195.5 75 132.0 76 81.0 77 60.0 78 51.0 79 38.5 80 27.5 81 22.0 82 15.0 83 13.0 84 10.5 85 7.0 86 5.5 87 3.0 88 1.5 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19222308326235302 2 0.0 3 0.0 4 0.02590376348519452 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.841388554403537E-5 48-49 0.003245973328470989 50-51 3.201157128669615E-5 52-53 0.0025609257029356918 54-55 0.009392195015516648 56-57 0.013899424252683467 58-59 0.014251551536837123 60-61 0.0031947548144122756 62-63 0.0022984308183847833 64-65 0.010173277354912033 66-67 0.0174463063512494 68-69 0.02551322231549683 70-71 0.02160140830426256 72-73 0.01717100683818381 74-75 0.021947133274158877 76-77 0.015333542646327452 78-79 0.006338291114765837 80-81 0.0032715825855003456 82-83 5.57001340388513E-4 84-85 0.0022472123043260693 86-87 0.015909750929487983 88-89 0.014757334363166922 90-91 0.018483481260938354 92-93 0.01884841317360669 94-95 0.017996905377380572 96-97 0.01982796725497959 98-99 0.02882962110079855 100-101 0.016530775412449888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7809676.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.04578576953941 #Duplication Level Percentage of deduplicated Percentage of total 1 88.53283314271847 37.224325358891186 2 5.877665086810519 4.942620941302725 3 1.8200601102482572 2.2957757244954746 4 0.73399029348162 1.234447945465982 5 0.5006886450084622 1.0525923752633382 6 0.37488860871720686 0.9457491677738618 7 0.219221493817651 0.6452137975594753 8 0.21112658319462982 0.7101586469804996 9 0.12803183550782968 0.4844879214698804 >10 1.2383054625281757 10.567077010764123 >50 0.1581926265232506 4.686157289329715 >100 0.168680060188273 14.595317649184326 >500 0.021173955860814123 6.234424143990689 >1k 0.014776675686506752 10.52988765090673 >5k 2.1325713772377757E-4 0.5701232486427598 >10k+ 1.5216257056452652E-4 3.281641127979277 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 205500 2.631351159766423 TruSeq Adapter, Index 7 (97% over 38bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18140 0.23227596125626723 No Hit TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA 12366 0.1583420362125138 No Hit GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC 10361 0.1326687560405835 No Hit TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG 10100 0.12932674799825242 No Hit GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC 9689 0.12406404567871958 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0021127637049219456 0.0 0.0 2 0.002356051646700836 0.0 0.0 3 0.00274019050214119 0.0 0.0 4 0.002881041415802653 0.0 0.0 5 0.003329203413816399 0.0 0.0 6 0.003995044096579679 0.0 0.0 7 0.0047761264359750645 0.0 0.0 8 0.005416357861708987 0.0 0.0 9 0.0062102448296190525 0.0 1.2804628514678458E-5 10-11 0.01053820926758037 0.0 1.2804628514678458E-5 12-13 0.014379597821983909 0.0 1.2804628514678458E-5 14-15 0.019475839970825935 0.0 1.2804628514678458E-5 16-17 0.028106159589719217 0.0 1.2804628514678458E-5 18-19 0.038868449856306456 0.0 1.2804628514678458E-5 20-21 0.04543722428433651 0.0 1.9206942772017686E-5 22-23 0.05157704365712483 0.0 2.5609257029356916E-5 24-25 0.05833148519861771 0.0 3.841388554403537E-5 26-27 0.06482983416981702 0.0 4.48161998013746E-5 28-29 0.07274309459188832 0.0 5.121851405871383E-5 30-31 0.12063880755104309 0.0 6.40231425733923E-5 32-33 0.13191328295821747 0.0 6.40231425733923E-5 34-35 0.14053720026285343 0.0 6.40231425733923E-5 36-37 0.1501790855344063 0.0 6.40231425733923E-5 38-39 0.16106301977188298 0.0 7.042545683073152E-5 40-41 0.1725039553497482 0.0 7.682777108807074E-5 42-43 0.18534699774997065 0.0 8.96323996027492E-5 44-45 0.19910557108899268 0.0 8.96323996027492E-5 46-47 0.21284493748524266 0.0 8.96323996027492E-5 48-49 0.22809525004622472 0.0 8.96323996027492E-5 50-51 0.2451510152277764 0.0 8.96323996027492E-5 52-53 0.2633335877186198 0.0 8.96323996027492E-5 54-55 0.28321917580191547 0.0 1.2164397088944536E-4 56-57 0.3041803526804441 0.0 1.280462851467846E-4 58-59 0.3262107160399484 0.0 1.280462851467846E-4 60-61 0.3508596259307044 0.0 1.4085091366146304E-4 62-63 0.3781526916097416 0.0 1.5365554217614149E-4 64-65 0.40832679870458133 0.0 1.5365554217614149E-4 66-67 0.4410554291880995 0.0 1.5365554217614149E-4 68-69 0.47724771168483815 0.0 1.5365554217614149E-4 70-71 0.5155463555722415 0.0 1.7286248494815918E-4 72-73 0.5584226541536422 0.0 1.8566711346283763E-4 74-75 0.6059662398286434 0.0 1.9847174197751608E-4 76-77 0.6588301486514934 0.0 2.0487405623485533E-4 78-79 0.7172448639354565 0.0 2.0487405623485533E-4 80-81 0.7830990683864478 0.0 2.0487405623485533E-4 82-83 0.855746128264476 0.0 2.0487405623485533E-4 84-85 0.9374140489310951 0.0 2.0487405623485533E-4 86-87 1.0253050190558481 0.0 2.0487405623485533E-4 88-89 1.1229467137945286 0.0 2.1127637049219456E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 35640 0.0 63.807842 4 TCGGAAG 35860 0.0 63.416386 3 GATCGGA 36510 0.0 62.40846 1 GAAGAGC 36900 0.0 61.58712 6 ATCGGAA 37245 0.0 61.045418 2 GGAAGAG 41930 0.0 54.4029 5 GCCGTCT 26260 0.0 43.041683 50-51 ATGCCGT 26280 0.0 42.799423 48-49 TATGCCG 26155 0.0 42.713406 48-49 TGCCGTC 26310 0.0 42.60783 50-51 TCGTATG 26635 0.0 42.52942 44-45 CGTATGC 26445 0.0 42.30529 46-47 TCTCGTA 26340 0.0 42.149223 42-43 CGTCTTC 26885 0.0 42.01593 52-53 CTCGTAT 26395 0.0 41.971428 44-45 CATCTCG 26370 0.0 41.937298 40-41 ATCTCGT 26360 0.0 41.63076 42-43 GTATGCC 27265 0.0 41.337803 46-47 TGTGTTT 88710 0.0 41.00401 1 GTCACGA 27895 0.0 40.146877 28-29 >>END_MODULE