FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_40255-1.341000000357f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_40255-1.341000000357f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7809676
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2055002.631351159766423TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181400.23227596125626723No Hit
TGTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTA123660.1583420362125138No Hit
GTGTTTGAGGTGGCAAGAAATGGGCTACATTTTCTACCCCAGAAAACTAC103610.1326687560405835No Hit
TGTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAG101000.12932674799825242No Hit
GTGTTTGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGC96890.12406404567871958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA356400.063.8078424
TCGGAAG358600.063.4163863
GATCGGA365100.062.408461
GAAGAGC369000.061.587126
ATCGGAA372450.061.0454182
GGAAGAG419300.054.40295
GCCGTCT262600.043.04168350-51
ATGCCGT262800.042.79942348-49
TATGCCG261550.042.71340648-49
TGCCGTC263100.042.6078350-51
TCGTATG266350.042.5294244-45
CGTATGC264450.042.3052946-47
TCTCGTA263400.042.14922342-43
CGTCTTC268850.042.0159352-53
CTCGTAT263950.041.97142844-45
CATCTCG263700.041.93729840-41
ATCTCGT263600.041.6307642-43
GTATGCC272650.041.33780346-47
TGTGTTT887100.041.004011
GTCACGA278950.040.14687728-29