##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_35011-1.341000000358a7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6163373 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6987553730725 33.0 33.0 33.0 33.0 33.0 2 32.72935598737899 33.0 33.0 33.0 33.0 33.0 3 32.76505462187669 33.0 33.0 33.0 33.0 33.0 4 36.69217991512115 37.0 37.0 37.0 37.0 37.0 5 36.68888285683829 37.0 37.0 37.0 37.0 37.0 6 36.56070207011648 37.0 37.0 37.0 37.0 37.0 7 36.48118927736485 37.0 37.0 37.0 37.0 37.0 8 36.42336866517733 37.0 37.0 37.0 37.0 37.0 9 36.455948390597165 37.0 37.0 37.0 37.0 37.0 10-11 36.40116028999056 37.0 37.0 37.0 37.0 37.0 12-13 36.38191871561238 37.0 37.0 37.0 37.0 37.0 14-15 38.23496054838803 40.0 37.0 40.0 37.0 40.0 16-17 38.239873037052924 40.0 37.0 40.0 37.0 40.0 18-19 38.226256061413125 40.0 37.0 40.0 37.0 40.0 20-21 38.193463303291885 40.0 37.0 40.0 37.0 40.0 22-23 38.053133162636755 40.0 37.0 40.0 35.0 40.0 24-25 38.0577274002401 40.0 37.0 40.0 35.0 40.0 26-27 37.98916688962359 40.0 37.0 40.0 35.0 40.0 28-29 37.8332113763032 40.0 37.0 40.0 33.0 40.0 30-31 37.81553915364201 40.0 37.0 40.0 33.0 40.0 32-33 37.69206171685536 40.0 37.0 40.0 33.0 40.0 34-35 37.553650979747616 40.0 37.0 40.0 33.0 40.0 36-37 37.34300114563892 40.0 37.0 40.0 33.0 40.0 38-39 37.41020476936898 40.0 37.0 40.0 33.0 40.0 40-41 37.256421767756066 40.0 37.0 40.0 33.0 40.0 42-43 37.09375369623095 40.0 37.0 40.0 33.0 40.0 44-45 37.04686865455004 38.5 37.0 40.0 33.0 40.0 46-47 37.01629740728008 38.5 37.0 40.0 33.0 40.0 48-49 36.77224897795412 37.0 37.0 40.0 33.0 40.0 50-51 36.66203862722571 37.0 37.0 40.0 33.0 40.0 52-53 36.55011403658354 37.0 37.0 40.0 33.0 40.0 54-55 36.328860349681904 37.0 37.0 40.0 33.0 40.0 56-57 36.19128194902369 37.0 37.0 40.0 33.0 40.0 58-59 35.939782810483806 37.0 37.0 40.0 33.0 40.0 60-61 35.68064483522254 37.0 37.0 40.0 27.0 40.0 62-63 35.43524592783854 37.0 33.0 40.0 27.0 40.0 64-65 35.18945056546148 37.0 33.0 38.5 27.0 40.0 66-67 34.73460749495447 37.0 33.0 37.0 27.0 40.0 68-69 34.515610689146996 37.0 33.0 37.0 27.0 40.0 70-71 34.132496929846695 37.0 33.0 37.0 27.0 40.0 72-73 33.576058758734874 37.0 33.0 37.0 27.0 38.5 74-75 33.03595912497913 37.0 33.0 37.0 22.0 37.0 76-77 30.498273185802645 33.0 30.0 35.0 22.0 37.0 78-79 32.68124466911219 35.0 33.0 37.0 24.5 37.0 80-81 32.98121685966434 37.0 33.0 37.0 27.0 37.0 82-83 32.86992690528385 37.0 33.0 37.0 27.0 37.0 84-85 32.675509173304945 37.0 33.0 37.0 22.0 37.0 86-87 32.550503433752915 37.0 33.0 37.0 22.0 37.0 88-89 32.3986960549037 35.0 33.0 37.0 22.0 37.0 90-91 32.25055168655216 33.0 33.0 37.0 22.0 37.0 92-93 32.11685581904584 33.0 33.0 37.0 22.0 37.0 94-95 31.984166948844408 33.0 33.0 37.0 22.0 37.0 96-97 31.812099316397045 33.0 33.0 37.0 22.0 37.0 98-99 31.644451747444137 33.0 33.0 37.0 18.5 37.0 100-101 30.001851015669505 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.10115781792394074 2306 2 0.09248802367184794 2306 3 0.08813524386071236 2306 4 0.15388171746783996 2306 5 0.14652897547637878 2306 6 0.18908040640209123 2306 7 0.2320743864462429 2306 8 0.16210216118904697 2306 9 0.19175537438080426 2306 10-11 0.25380665772682676 2306 12-13 0.20893409091826953 2306 14-15 0.4578257722422663 2306 16-17 0.4474600180650441 2306 18-19 0.3618654742684342 2306 20-21 0.40210918524388717 2306 22-23 0.43036926600678527 2306 24-25 0.4832042944908963 2306 26-27 0.4824232889210336 2306 28-29 0.46716332807253735 2306 30-31 0.4382287268449119 2306 32-33 0.5249844545380213 2306 34-35 0.5309000507520878 2306 36-37 0.4640337652088746 2306 38-39 0.5344655100149609 2306 40-41 0.541564831943532 2306 42-43 0.5716140569510202 2306 44-45 0.5938879365027745 2306 46-47 0.5674059978041583 2306 48-49 0.5493254372538559 2306 50-51 0.6512567189771659 2306 52-53 0.5040144478903557 2306 54-55 0.5673298315512483 2306 56-57 0.5976201440207305 2306 58-59 0.6113081549269452 2306 60-61 0.5965562054524227 2306 62-63 0.5562461635044897 2306 64-65 0.5195018902744053 2306 66-67 0.5415602179259551 2306 68-69 0.6217917464631384 2306 70-71 0.5457051722832418 2306 72-73 0.47216339333686363 2306 74-75 0.5322388620136564 2306 76-77 0.5758216841799886 2306 78-79 0.6494534877643261 2306 80-81 0.6530058713583244 2306 82-83 0.690812419455682 2306 84-85 0.671383865759438 2306 86-87 0.6213825383230116 2306 88-89 0.6359484056827895 2306 90-91 0.6001117322537723 2306 92-93 0.7415858925367189 2306 94-95 0.5903140690552604 2306 96-97 0.673131291012325 2306 98-99 0.8024001861228633 2306 100-101 0.6237115632443988 2307 1 0.11447410189504126 2307 2 0.1175922619600911 2307 3 0.10130130541065796 2307 4 0.15382876336762052 2307 5 0.16487483059637498 2307 6 0.16189679066082618 2307 7 0.17092484954842746 2307 8 0.1893546172606051 2307 9 0.1945103346703334 2307 10-11 0.20110420560824593 2307 12-13 0.1937939562532094 2307 14-15 0.4569723518858382 2307 16-17 0.4654991342369641 2307 18-19 0.3905261842491399 2307 20-21 0.44039780998782163 2307 22-23 0.4832506472429614 2307 24-25 0.49201615869910853 2307 26-27 0.3938983402470271 2307 28-29 0.45407045001811497 2307 30-31 0.4834551748352993 2307 32-33 0.5736137376537727 2307 34-35 0.5007346778841608 2307 36-37 0.4189948862155859 2307 38-39 0.5021027742426369 2307 40-41 0.49290078028039375 2307 42-43 0.5022766316592637 2307 44-45 0.5484551068744139 2307 46-47 0.5675625143310867 2307 48-49 0.5277657792109949 2307 50-51 0.3987114383441295 2307 52-53 0.4753125359036048 2307 54-55 0.5073578266188932 2307 56-57 0.6361059298860496 2307 58-59 0.5567460151948254 2307 60-61 0.5898896790676673 2307 62-63 0.46841414710247165 2307 64-65 0.33939331300150144 2307 66-67 0.48757144568163113 2307 68-69 0.6374286974255128 2307 70-71 0.47567065735812264 2307 72-73 0.36473248380559653 2307 74-75 0.3046381909272782 2307 76-77 0.43547680527347055 2307 78-79 0.3787648549936691 2307 80-81 0.4042403289815226 2307 82-83 0.3742094231268851 2307 84-85 0.4374623331451488 2307 86-87 0.43755574709714296 2307 88-89 0.3105130370446787 2307 90-91 0.3184960209023018 2307 92-93 0.4956740884267532 2307 94-95 0.34039770401793845 2307 96-97 0.43359304705019497 2307 98-99 0.5662017433264417 2307 100-101 0.4282039104014501 2201 1 0.11808070243616697 2201 2 0.10096107100780216 2201 3 0.05432786086959851 2201 4 0.13155407946134545 2201 5 0.1462038993358945 2201 6 0.17617613786625697 2201 7 0.1618458886729428 2201 8 0.1791494432302514 2201 9 0.18189868908262952 2201 10-11 0.2033702888706017 2201 12-13 0.2004826915197171 2201 14-15 0.34559945015363525 2201 16-17 0.32911549788109085 2201 18-19 0.30854660443538506 2201 20-21 0.38422488681872125 2201 22-23 0.3488494049392372 2201 24-25 0.3173761617955506 2201 26-27 0.3820247526251208 2201 28-29 0.3336633770370483 2201 30-31 0.3276642968252901 2201 32-33 0.35014696359556297 2201 34-35 0.44173159400185824 2201 36-37 0.34506850715786896 2201 38-39 0.3931634637983592 2201 40-41 0.3801861314964441 2201 42-43 0.3118728868322407 2201 44-45 0.3613562151328864 2201 46-47 0.42088943213293106 2201 48-49 0.39055049269266817 2201 50-51 0.3756825152485632 2201 52-53 0.4457323532162931 2201 54-55 0.390558130877686 2201 56-57 0.3786880363266576 2201 58-59 0.32963438818315893 2201 60-61 0.457932254585117 2201 62-63 0.46862760980274487 2201 64-65 0.290946470974383 2201 66-67 0.3853733623928619 2201 68-69 0.46318183952784153 2201 70-71 0.27884160758299714 2201 72-73 0.13776810592768385 2201 74-75 0.22070294844257887 2201 76-77 0.07620904835480147 2201 78-79 0.3504652543333222 2201 80-81 0.2692940383457767 2201 82-83 0.26875289450158846 2201 84-85 0.35163874187923483 2201 86-87 0.4145609340206988 2201 88-89 0.3246589215187683 2201 90-91 0.39629961528278557 2201 92-93 0.42140738587660564 2201 94-95 0.39211412406333324 2201 96-97 0.29662572160060563 2201 98-99 0.2639624432251999 2201 100-101 0.3315400644456581 1101 1 0.07078977542043674 1101 2 0.04776688781428362 1101 3 0.10015107849985583 1101 4 0.13459566523263078 1101 5 0.1261873674444658 1101 6 0.18673142797070597 1101 7 0.16787490819065454 1101 8 0.1742556807935287 1101 9 0.15821727879131942 1101 10-11 0.1904081569824001 1101 12-13 0.17668928774440218 1101 14-15 0.29440921445321777 1101 16-17 0.2838518646148884 1101 18-19 0.2761020215531502 1101 20-21 0.3242928288603295 1101 22-23 0.3254074853043889 1101 24-25 0.34974411331275945 1101 26-27 0.2930644876309927 1101 28-29 0.3110345240479049 1101 30-31 0.41743881795486004 1101 32-33 0.3559323191622781 1101 34-35 0.44365657147211124 1101 36-37 0.351754931450877 1101 38-39 0.3687428158691759 1101 40-41 0.4175797376866228 1101 42-43 0.4739736265335779 1101 44-45 0.4666766213335549 1101 46-47 0.5214722619492065 1101 48-49 0.5014233967209947 1101 50-51 0.4475429578597172 1101 52-53 0.4594721896312066 1101 54-55 0.4801830182289706 1101 56-57 0.5062489371937815 1101 58-59 0.5737744173357413 1101 60-61 0.5245001331200712 1101 62-63 0.542667439564589 1101 64-65 0.4691165720406971 1101 66-67 0.5557896031617204 1101 68-69 0.537776354568841 1101 70-71 0.46379494531139187 1101 72-73 0.2779581420357573 1101 74-75 0.3110984033820898 1101 76-77 0.13046342981004955 1101 78-79 0.38871466815680833 1101 80-81 0.46232074541477886 1101 82-83 0.28642486145393775 1101 84-85 0.3853570666744446 1101 86-87 0.4654538376055015 1101 88-89 0.3971794099756849 1101 90-91 0.4308742517323836 1101 92-93 0.49615875227775774 1101 94-95 0.4944082868944051 1101 96-97 0.4601617318907074 1101 98-99 0.6056923846982691 1101 100-101 0.5319708815041508 2304 1 0.04971097613574216 2304 2 0.036030409357657334 2304 3 0.08168027723570503 2304 4 0.10233793207574848 2304 5 0.07403947866846039 2304 6 0.07991717744304339 2304 7 0.06377107713356622 2304 8 0.0806804898779987 2304 9 0.09604813344058982 2304 10-11 0.09584792814619192 2304 12-13 0.13318731391960625 2304 14-15 0.1721980433171666 2304 16-17 0.2182500636169209 2304 18-19 0.21339032743788522 2304 20-21 0.23940059257120083 2304 22-23 0.22720904561945332 2304 24-25 0.26160370745089523 2304 26-27 0.2808367836034975 2304 28-29 0.2920168627209918 2304 30-31 0.23115361995380113 2304 32-33 0.19929685629755056 2304 34-35 0.18802147975392813 2304 36-37 0.1969474965233644 2304 38-39 0.11214825410326767 2304 40-41 0.2573790561266094 2304 42-43 0.16716102554610046 2304 44-45 0.2870445812647233 2304 46-47 0.21137505154660374 2304 48-49 0.2555656920561802 2304 50-51 0.30364899330233897 2304 52-53 0.26373208968407624 2304 54-55 0.19754712798337692 2304 56-57 0.1679604476102483 2304 58-59 0.18385089601730442 2304 60-61 0.32632981059747124 2304 62-63 0.2351447115250238 2304 64-65 0.1964448417854996 2304 66-67 0.2023700688569008 2304 68-69 0.2337587869658151 2304 70-71 0.23464348378129074 2304 72-73 0.06619823261051039 2304 74-75 0.115323888028243 2304 76-77 0.0196804230731793 2304 78-79 0.2916925380755657 2304 80-81 0.25648167305619296 2304 82-83 0.3115871349701962 2304 84-85 0.3928251349504137 2304 86-87 0.30642920322232925 2304 88-89 0.14938094629742693 2304 90-91 0.22143644502100557 2304 92-93 0.36381314361617 2304 94-95 0.3291171243059736 2304 96-97 0.4076746376682152 2304 98-99 0.5156617557360939 2304 100-101 0.42254229722202297 2202 1 0.04850540860065422 2202 2 0.06077915408096857 2202 3 0.05921477510986506 2202 4 0.08484279336860823 2202 5 0.07982152838422962 2202 6 0.05315841808413779 2202 7 0.1067002022277066 2202 8 0.07342660808290447 2202 9 0.1575291131065839 2202 10-11 0.16821641814355104 2202 12-13 0.07696616211792673 2202 14-15 0.15416244687769876 2202 16-17 0.1476884081381442 2202 18-19 0.12400345153591275 2202 20-21 0.1578240858461939 2202 22-23 0.15756821594408876 2202 24-25 0.12790853609151753 2202 26-27 0.15847347135530043 2202 28-29 0.1719321393010489 2202 30-31 0.13310390777046877 2202 32-33 0.12789369592631772 2202 34-35 0.10904687816483971 2202 36-37 0.10774856395324406 2202 38-39 0.15106258972566167 2202 40-41 0.12996502537781396 2202 42-43 0.23550434988996471 2202 44-45 0.22794546963135076 2202 46-47 0.2248758084218423 2202 48-49 0.26250439302165773 2202 50-51 0.08616215554059181 2202 52-53 0.27191689768540783 2202 54-55 0.4079430003451705 2202 56-57 0.3108595679582393 2202 58-59 0.270469916820133 2202 60-61 0.21326831438102545 2202 62-63 0.34101041188075243 2202 64-65 0.2734507611041792 2202 66-67 0.24257477403131844 2202 68-69 0.29246201796402005 2202 70-71 0.15877597983145364 2202 72-73 0.19730870054390692 2202 74-75 0.24576921168651467 2202 76-77 0.16601209465468614 2202 78-79 0.26396100305978365 2202 80-81 0.17516563951816977 2202 82-83 0.26403174960087483 2202 84-85 0.344918372768646 2202 86-87 0.19338811464572814 2202 88-89 0.19954068434055472 2202 90-91 0.16304760198801915 2202 92-93 0.39794388709130857 2202 94-95 0.155492888799607 2202 96-97 0.2517577527455188 2202 98-99 0.33777646018520713 2202 100-101 0.41713944351694465 1102 1 0.05640317609325507 1102 2 0.004126904851986524 1102 3 0.0350168196832783 1102 4 0.10347978279276049 1102 5 0.07350419043493162 1102 6 0.062301799757669585 1102 7 2.9573938703464364E-4 1102 8 0.05891601337638974 1102 9 0.10295219477633566 1102 10-11 0.015610392745927015 1102 12-13 0.019975564572263238 1102 14-15 0.05062041239478532 1102 16-17 0.13616664481986618 1102 18-19 0.08750884880304 1102 20-21 0.04604927667368486 1102 22-23 0.05766219892324642 1102 24-25 0.08779347048727715 1102 26-27 0.060250659277137686 1102 28-29 0.08679700459797601 1102 30-31 0.07333768703404075 1102 32-33 0.1199019294683481 1102 34-35 -0.008785556849581155 1102 36-37 0.06560449551175651 1102 38-39 0.07958948525955378 1102 40-41 0.004621001953523773 1102 42-43 0.11887100916034399 1102 44-45 0.06864969133590648 1102 46-47 0.05402722157159445 1102 48-49 0.02965017198852138 1102 50-51 1.118258088439461E-4 1102 52-53 -0.019834741061856676 1102 54-55 0.03050969005330728 1102 56-57 0.008641256320338186 1102 58-59 0.08443028186863444 1102 60-61 -0.12341318323351658 1102 62-63 0.04762028164422816 1102 64-65 -0.07941366553091456 1102 66-67 0.04980319855110338 1102 68-69 0.10506397007315371 1102 70-71 -0.0028964376109215095 1102 72-73 -0.11851957421698955 1102 74-75 -0.2383609973428804 1102 76-77 -0.2267956736244372 1102 78-79 -0.0437584061918912 1102 80-81 -0.08357786288328128 1102 82-83 -0.06342179924374136 1102 84-85 0.06677519443981339 1102 86-87 0.053540834189263364 1102 88-89 -0.018098365387459125 1102 90-91 -0.06735852150104193 1102 92-93 -0.006135252364153132 1102 94-95 -0.1835232841380261 1102 96-97 -0.060472487808702624 1102 98-99 -0.03910451026778361 1102 100-101 -0.03786552180255143 2305 1 0.05268251469436791 2305 2 0.051530914216925794 2305 3 0.04255866925893059 2305 4 0.06858411407751674 2305 5 0.058515750282751355 2305 6 0.0038023850664146153 2305 7 -0.013172946594821155 2305 8 0.005617066932146031 2305 9 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-0.213944100334313 1213 98-99 -0.0010411505955580935 1213 100-101 0.10301097805111681 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 20.0 10 48.0 11 39.0 12 66.0 13 186.0 14 541.0 15 1226.0 16 2333.0 17 3864.0 18 6113.0 19 8735.0 20 12245.0 21 15592.0 22 20589.0 23 26407.0 24 33957.0 25 43075.0 26 56786.0 27 79891.0 28 123811.0 29 166532.0 30 140812.0 31 139211.0 32 183782.0 33 260070.0 34 393150.0 35 666901.0 36 1255571.0 37 1946606.0 38 574734.0 39 473.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.21435365613176 25.250253240428925 22.052604104941597 15.48278899849771 2 28.94074397249688 25.832007895676607 31.591143356081158 13.636104775745359 3 35.80873654734185 19.466516143027526 28.26327402219531 16.461473287435304 4 27.995295489298 19.871948632741436 31.996921002585953 20.13583487537461 5 34.65279482517122 18.46675513554023 30.269821411100708 16.610628628187847 6 28.982815091671394 19.51958773223688 34.709111390792025 16.788485785299706 7 31.083239648160188 23.179823775066023 27.49515565583975 18.24178092093404 8 27.767944597868734 29.273500078609555 25.317565560286553 17.64098976323516 9 32.029166496981446 23.579215471787933 25.82894139296778 18.562676638262847 10-11 24.46709780504928 30.064787576542912 29.20257300669617 16.26554161171164 12-13 22.650706033855165 28.534075416172282 29.130274932248952 19.684943617723608 14-15 21.871643984551966 28.820412134719092 29.682642929447884 19.625300951281062 16-17 23.151462681229905 27.319975604267338 30.31209534130094 19.216466373201815 18-19 20.66347436703896 27.44685418195524 32.38396410536893 19.50570734563688 20-21 22.781089186067437 27.709551247344592 31.913474975472035 17.595884591115937 22-23 20.842232005104997 31.64501807695234 29.693302676959515 17.819447240983145 24-25 20.679926397445037 27.42800898144571 31.775936650272506 20.116127970836747 26-27 20.661397582135628 27.674992248562596 29.509012354111945 22.154597815189835 28-29 22.31793370286043 29.647118550183478 29.438547691337195 18.596400055618894 30-31 19.96170765585662 28.036425833711508 31.39570653926024 20.606159971171632 32-33 19.999373719552587 30.09713187892409 29.696685564868456 20.20680883665486 34-35 22.29100039864535 29.87669251885291 29.76469215801153 18.06761492449021 36-37 20.225078053851355 29.728567458111005 31.850863155613006 18.19549133242463 38-39 20.7938494068102 27.500169144395446 31.1890177667326 20.516963682061753 40-41 22.568389094737572 27.71693356868066 30.78427997137282 18.930397365208954 42-43 20.37105331772067 29.824845908238885 31.17176260466469 18.63233816937576 44-45 20.13232040312991 27.794318143652834 31.47627930355667 20.597082149660583 46-47 22.273131419986143 27.686200893342438 29.371236839586135 20.66943084708528 48-49 20.36864372025737 30.06912090036542 30.754318403312965 18.80791697606425 50-51 22.693626714099615 27.936759242148 30.3133173799321 19.05629666382028 52-53 20.57260768844794 27.77799546745749 28.381666526723304 23.26773031737127 54-55 22.627176055098197 27.98790812315476 30.190099816398835 19.194816005348205 56-57 20.487726829876213 28.19017393454785 30.29375187016799 21.028347365407946 58-59 20.466243008537422 28.193711789145866 30.60538909756989 20.734656104746826 60-61 22.230389007226144 28.463895887445045 30.478457860038393 18.827257245290415 62-63 20.135972401511154 28.30014135431452 30.439885800251687 21.12400044392264 64-65 22.116569772913582 28.033119554797164 30.420035555474783 19.430275116814467 66-67 20.330757310977337 31.394909371321916 28.59020505674546 19.684128260955287 68-69 20.178110311090546 31.769837835372805 28.55772908014477 19.49432277339188 70-71 19.90535201777512 32.44106125244874 28.31059202010225 19.342994709673892 72-73 19.941910180907342 32.24226750380354 28.12388156570375 19.691940749585367 74-75 20.181684519206662 31.536784400658547 28.381676242746938 19.89985483738785 76-77 20.08099690091194 31.22134868911074 28.52893277325379 20.16872163672353 78-79 19.888585014266603 31.143453791541287 28.70945863172045 20.258502562471655 80-81 19.778304381828864 31.221447369702005 28.479762070878994 20.52048617759014 82-83 19.495578163991937 30.954555679974156 28.61181372061077 20.938052435423135 84-85 19.664384905230563 30.678114576777304 28.84664528498978 20.810855233002354 86-87 19.96932536990532 30.574523342812558 29.10940934501153 20.346741942270583 88-89 20.217519852422857 30.662881713701907 28.734313113309074 20.38528532056616 90-91 20.272793356553624 30.497858464317652 28.61000309382066 20.61934508530807 92-93 19.853358864032636 31.023962090497886 28.673620993745065 20.449058051724414 94-95 19.557402340456917 31.1343279077085 28.73055048755327 20.577719264281313 96-97 19.787412119862243 31.261510274607556 28.552702523426248 20.398375082103954 98-99 20.271006189027403 30.694521523158517 28.196075697820568 20.83839658999351 100-101 20.432367250494412 30.623175115706164 27.667578598323338 21.276879035476085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 199.0 1 115.5 2 49.5 3 88.5 4 106.5 5 83.0 6 66.0 7 70.0 8 69.5 9 65.5 10 60.5 11 52.5 12 66.0 13 89.5 14 98.0 15 117.5 16 164.0 17 252.5 18 359.0 19 517.5 20 833.5 21 1356.5 22 2261.5 23 3744.0 24 5730.5 25 8835.0 26 14054.5 27 20958.5 28 29019.0 29 38873.0 30 50085.0 31 62300.5 32 80505.5 33 109462.5 34 147893.5 35 197408.0 36 267924.0 37 341756.5 38 403489.0 39 435634.5 40 442263.5 41 431132.0 42 400715.0 43 371641.5 44 336091.0 45 302197.0 46 282327.0 47 254534.5 48 217153.5 49 187168.5 50 156545.0 51 128142.5 52 104531.0 53 81479.0 54 64890.5 55 51485.5 56 37718.5 57 26144.0 58 18068.5 59 13094.0 60 9587.0 61 6781.0 62 4675.5 63 3155.0 64 2148.0 65 1510.0 66 1011.0 67 717.0 68 513.0 69 364.0 70 256.0 71 168.5 72 121.5 73 83.0 74 61.0 75 53.5 76 33.5 77 26.0 78 18.5 79 13.5 80 13.0 81 6.5 82 3.5 83 4.5 84 5.0 85 4.0 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19638597242126998 2 0.0 3 0.0 4 0.02639788310718822 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.867464617182831E-5 48-49 0.0030259405036819936 50-51 9.734929234365663E-5 52-53 0.0026852179804791954 54-55 0.009053484187960066 56-57 0.013385527697252786 58-59 0.013904723923085623 60-61 0.0031963017652833926 62-63 0.0022228088418468264 64-65 0.009880953172881148 66-67 0.01671162851899439 68-69 0.02462125852191649 70-71 0.020711061946112948 72-73 0.016541267257392987 74-75 0.021384394551489907 76-77 0.014845767082407636 78-79 0.006133005417650368 80-81 0.0031314022370542882 82-83 6.408828412624062E-4 84-85 0.0024661820727059683 86-87 0.015437975277498213 88-89 0.014618618733605772 90-91 0.017677009001402316 92-93 0.01814753058106332 94-95 0.017393073565399986 96-97 0.01972134414061911 98-99 0.02827185698480361 100-101 0.016159982529047 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6163373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.35552202478271 #Duplication Level Percentage of deduplicated Percentage of total 1 91.66558362012836 50.74196232998577 2 4.038554162727488 4.471125478062787 3 1.4255929590635288 2.36743327331449 4 0.6399295366223569 1.4169453423523146 5 0.3401745129180732 0.9415268871053065 6 0.24266785794018983 0.805980357294901 7 0.1993551369569114 0.7724785372200317 8 0.14375598135536982 0.6366149913689142 9 0.12559123022245136 0.6256951299628616 >10 0.945490048216012 10.633605271367834 >50 0.12758518421807197 4.951914758161744 >100 0.08858779342646388 9.606544623348464 >500 0.01350166392515632 5.250467397536627 >1k 0.0035129888711529353 2.672728461372604 >5k 0.0 0.0 >10k+ 1.1732340845548566E-4 4.104977161545239 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 219606 3.563081449070176 TruSeq Adapter, Index 7 (97% over 35bp) GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC 12291 0.1994200253659806 No Hit TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG 10500 0.1703612616013991 No Hit TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA 10431 0.16924174473944706 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 6.003206361192159E-4 0.0 0.0 2 6.327704002337681E-4 0.0 0.0 3 6.652201643483203E-4 0.0 0.0 4 7.625694566919769E-4 0.0 0.0 5 0.0010383924516656708 0.0 0.0 6 0.0011519666260666034 0.0 0.0 7 0.0012817656825248123 0.0 1.6224882057276106E-5 8 0.0014926891492694017 0.0 1.6224882057276106E-5 9 0.0016062633236703344 0.0 1.6224882057276106E-5 10-11 0.0027501175087083 8.112441028638053E-6 1.6224882057276106E-5 12-13 0.0038858592527176274 1.6224882057276106E-5 1.6224882057276106E-5 14-15 0.005200074699356991 1.6224882057276106E-5 3.244976411455221E-5 16-17 0.006717101171712307 1.6224882057276106E-5 4.056220514319026E-5 18-19 0.008688424341671355 1.6224882057276106E-5 4.867464617182831E-5 20-21 0.010602960424429934 1.6224882057276106E-5 5.678708720046637E-5 22-23 0.013101592261250456 1.6224882057276106E-5 6.489952822910442E-5 24-25 0.016005846149502876 1.6224882057276106E-5 6.489952822910442E-5 26-27 0.01870728901203935 1.6224882057276106E-5 6.489952822910442E-5 28-29 0.02195226542349457 1.6224882057276106E-5 6.489952822910442E-5 30-31 0.03085161323191051 1.6224882057276106E-5 7.301196925774248E-5 32-33 0.03426695090496713 1.6224882057276106E-5 8.112441028638053E-5 34-35 0.03793377424991154 1.6224882057276106E-5 8.112441028638053E-5 36-37 0.041746621533371414 1.6224882057276106E-5 8.112441028638053E-5 38-39 0.046127339688835964 1.6224882057276106E-5 8.112441028638053E-5 40-41 0.050678419105901915 1.6224882057276106E-5 8.112441028638053E-5 42-43 0.05573246986674342 1.6224882057276106E-5 8.112441028638053E-5 44-45 0.06077840818655629 1.6224882057276106E-5 8.112441028638053E-5 46-47 0.0666193657271757 1.6224882057276106E-5 8.112441028638053E-5 48-49 0.07308498122700022 1.6224882057276106E-5 8.112441028638053E-5 50-51 0.07929911105493696 1.6224882057276106E-5 8.112441028638053E-5 52-53 0.08608111175487837 1.6224882057276106E-5 8.923685131501859E-5 54-55 0.09358511970636857 1.6224882057276106E-5 9.734929234365663E-5 56-57 0.10130005112460336 1.6224882057276106E-5 9.734929234365663E-5 58-59 0.10876349687095038 1.6224882057276106E-5 9.734929234365663E-5 60-61 0.11677047616621614 1.6224882057276106E-5 9.734929234365663E-5 62-63 0.12544267562583022 1.6224882057276106E-5 1.2168661542957079E-4 64-65 0.13487744454213627 1.6224882057276106E-5 1.379114974868469E-4 66-67 0.14511534512027746 1.6224882057276106E-5 1.4602393851548495E-4 68-69 0.1563754132680271 1.6224882057276106E-5 1.4602393851548495E-4 70-71 0.1684629504006978 1.6224882057276106E-5 1.54136379544123E-4 72-73 0.1815969924260628 1.6224882057276106E-5 1.6224882057276106E-4 74-75 0.19748926440116477 1.6224882057276106E-5 1.6224882057276106E-4 76-77 0.21558000789502763 1.6224882057276106E-5 1.703612616013991E-4 78-79 0.23517155297918851 1.6224882057276106E-5 1.7847370263003714E-4 80-81 0.2573022921053131 1.6224882057276106E-5 1.7847370263003714E-4 82-83 0.28217503629911733 1.6224882057276106E-5 1.7847370263003714E-4 84-85 0.31027653202231964 1.6224882057276106E-5 1.865861436586752E-4 86-87 0.3431643679524182 1.6224882057276106E-5 1.9469858468731325E-4 88-89 0.379816376519805 1.6224882057276106E-5 2.1092346674458936E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28750 0.0 83.077515 3 GATCGGA 29085 0.0 82.48059 1 CGGAAGA 28905 0.0 82.43481 4 ATCGGAA 29365 0.0 81.3376 2 GAAGAGC 30815 0.0 77.36872 6 GGAAGAG 34730 0.0 68.8113 5 AGAGCAC 35325 0.0 67.477486 8 GAGCACA 35405 0.0 67.45916 9 AAGAGCA 37285 0.0 63.91759 7 GCCGTCT 25915 0.0 45.621525 50-51 ATGCCGT 25875 0.0 45.524433 48-49 TATGCCG 25785 0.0 45.416233 48-49 TCGTATG 26025 0.0 45.415333 44-45 CGTATGC 25830 0.0 45.29886 46-47 TGCCGTC 25950 0.0 45.248817 50-51 TCTCGTA 26005 0.0 45.112373 42-43 CGTCTTC 26245 0.0 45.023296 52-53 CTCGTAT 25925 0.0 45.004257 44-45 CGTATCT 25855 0.0 44.975857 38-39 AATTCGT 25740 0.0 44.92771 34-35 >>END_MODULE