FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_35011-1.341000000358a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_35011-1.341000000358a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6163373
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2196063.563081449070176TruSeq Adapter, Index 7 (97% over 35bp)
GTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC122910.1994200253659806No Hit
TGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAG105000.1703612616013991No Hit
TTGTGTTTGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCA104310.16924174473944706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG287500.083.0775153
GATCGGA290850.082.480591
CGGAAGA289050.082.434814
ATCGGAA293650.081.33762
GAAGAGC308150.077.368726
GGAAGAG347300.068.81135
AGAGCAC353250.067.4774868
GAGCACA354050.067.459169
AAGAGCA372850.063.917597
GCCGTCT259150.045.62152550-51
ATGCCGT258750.045.52443348-49
TATGCCG257850.045.41623348-49
TCGTATG260250.045.41533344-45
CGTATGC258300.045.2988646-47
TGCCGTC259500.045.24881750-51
TCTCGTA260050.045.11237342-43
CGTCTTC262450.045.02329652-53
CTCGTAT259250.045.00425744-45
CGTATCT258550.044.97585738-39
AATTCGT257400.044.9277134-35
Produced by FastQC (version 0.11.2)