##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_35010-1.341000000357eb.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7011968 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.700630978350155 33.0 33.0 33.0 33.0 33.0 2 32.73198052244391 33.0 33.0 33.0 33.0 33.0 3 32.76467918849601 33.0 33.0 33.0 33.0 33.0 4 36.694929155409724 37.0 37.0 37.0 37.0 37.0 5 36.68690501725051 37.0 37.0 37.0 37.0 37.0 6 36.565742028486156 37.0 37.0 37.0 37.0 37.0 7 36.510578200014606 37.0 37.0 37.0 37.0 37.0 8 36.44901531210639 37.0 37.0 37.0 37.0 37.0 9 36.463825277012106 37.0 37.0 37.0 37.0 37.0 10-11 36.42035909176995 37.0 37.0 37.0 37.0 37.0 12-13 36.40343081999234 37.0 37.0 37.0 37.0 37.0 14-15 38.28412016426772 40.0 37.0 40.0 37.0 40.0 16-17 38.27335663825049 40.0 37.0 40.0 37.0 40.0 18-19 38.27445105282854 40.0 37.0 40.0 37.0 40.0 20-21 38.21241062423559 40.0 37.0 40.0 37.0 40.0 22-23 38.101755455814974 40.0 37.0 40.0 37.0 40.0 24-25 38.0838240419808 40.0 37.0 40.0 37.0 40.0 26-27 38.01215835554298 40.0 37.0 40.0 35.0 40.0 28-29 37.88088016089064 40.0 37.0 40.0 33.0 40.0 30-31 37.8409007285829 40.0 37.0 40.0 33.0 40.0 32-33 37.72842117933225 40.0 37.0 40.0 33.0 40.0 34-35 37.63033823029426 40.0 37.0 40.0 33.0 40.0 36-37 37.527013386256186 40.0 37.0 40.0 33.0 40.0 38-39 37.47235490806575 40.0 37.0 40.0 33.0 40.0 40-41 37.41377171145105 40.0 37.0 40.0 33.0 40.0 42-43 37.27382205680345 40.0 37.0 40.0 33.0 40.0 44-45 37.159406246577284 40.0 37.0 40.0 33.0 40.0 46-47 37.111144118170536 40.0 37.0 40.0 33.0 40.0 48-49 36.923849837877185 37.0 37.0 40.0 33.0 40.0 50-51 36.7922044852458 37.0 37.0 40.0 33.0 40.0 52-53 36.66998009688578 37.0 37.0 40.0 33.0 40.0 54-55 36.502483824797835 37.0 37.0 40.0 33.0 40.0 56-57 36.34600935144028 37.0 37.0 40.0 33.0 40.0 58-59 36.13399027206057 37.0 37.0 40.0 33.0 40.0 60-61 35.89611440040799 37.0 37.0 40.0 30.0 40.0 62-63 35.65780084849218 37.0 35.0 40.0 27.0 40.0 64-65 35.44524768224841 37.0 33.0 40.0 27.0 40.0 66-67 35.13129523694347 37.0 33.0 38.5 27.0 40.0 68-69 34.87489425222705 37.0 33.0 37.0 27.0 40.0 70-71 34.558904361799705 37.0 33.0 37.0 27.0 40.0 72-73 34.21105109150527 37.0 33.0 37.0 27.0 40.0 74-75 33.83808917838758 37.0 33.0 37.0 27.0 38.5 76-77 31.244403639605885 33.0 30.0 35.0 24.5 37.0 78-79 33.50774639872857 37.0 33.0 37.0 27.0 37.0 80-81 33.825321364843646 37.0 33.0 37.0 27.0 37.0 82-83 33.7118050881008 37.0 33.0 37.0 27.0 37.0 84-85 33.52389685748709 37.0 33.0 37.0 27.0 37.0 86-87 33.39589370630328 37.0 33.0 37.0 27.0 37.0 88-89 33.24470883209963 37.0 33.0 37.0 27.0 37.0 90-91 33.08459629308064 37.0 33.0 37.0 27.0 37.0 92-93 32.95232543845037 35.0 33.0 37.0 27.0 37.0 94-95 32.831980051819976 33.0 33.0 37.0 27.0 37.0 96-97 32.667800109755206 33.0 33.0 37.0 27.0 37.0 98-99 32.50648427089228 33.0 33.0 37.0 27.0 37.0 100-101 30.802246459196617 33.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1243310085129039 2306 2 0.09384742283420167 2306 3 0.10801632962664343 2306 4 0.12165841700374358 2306 5 0.15590111003754004 2306 6 0.17021844883213788 2306 7 0.18963930390019357 2306 8 0.20042837680104952 2306 9 0.20767318984982808 2306 10-11 0.21111200490252457 2306 12-13 0.21102383846945116 2306 14-15 0.4513298242510686 2306 16-17 0.5043030414669474 2306 18-19 0.37539937588501715 2306 20-21 0.47195678976097355 2306 22-23 0.5224600183655994 2306 24-25 0.4343672295605572 2306 26-27 0.49130696130553275 2306 28-29 0.47334992376938345 2306 30-31 0.4821864329944958 2306 32-33 0.5270615101587097 2306 34-35 0.6198723820398584 2306 36-37 0.5548141932302286 2306 38-39 0.594543562133758 2306 40-41 0.5919690720757558 2306 42-43 0.5963488355598585 2306 44-45 0.6481757359209226 2306 46-47 0.5876928705714022 2306 48-49 0.5931722853505406 2306 50-51 0.6096577270759553 2306 52-53 0.609949852807901 2306 54-55 0.619955664011421 2306 56-57 0.7015314045404466 2306 58-59 0.6765405129372795 2306 60-61 0.6646891042325436 2306 62-63 0.5845849424537803 2306 64-65 0.6125355964367643 2306 66-67 0.6028305944977248 2306 68-69 0.6466976742847521 2306 70-71 0.6979350042053838 2306 72-73 0.47241803931525084 2306 74-75 0.5539374260701777 2306 76-77 0.5009725453677483 2306 78-79 0.5862503822248755 2306 80-81 0.5498754272816129 2306 82-83 0.505976958493477 2306 84-85 0.6274198974854315 2306 86-87 0.6557770774454497 2306 88-89 0.5676100545897569 2306 90-91 0.5767289781621585 2306 92-93 0.7649574274801125 2306 94-95 0.65995136007448 2306 96-97 0.7108590462845683 2306 98-99 0.8017328802163703 2306 100-101 0.5942979429959756 2307 1 0.10515570003811092 2307 2 0.08522698415951169 2307 3 0.10833854847437863 2307 4 0.15558316465898514 2307 5 0.14082770786848187 2307 6 0.16911682261754635 2307 7 0.14734272472843202 2307 8 0.1767143665332469 2307 9 0.21055916163079047 2307 10-11 0.19587197019680502 2307 12-13 0.16338211595412133 2307 14-15 0.39599664168903104 2307 16-17 0.38827990539341783 2307 18-19 0.40306688395804 2307 20-21 0.43189707127157106 2307 22-23 0.4564294831097797 2307 24-25 0.539237098985673 2307 26-27 0.3641154203351533 2307 28-29 0.375089923435759 2307 30-31 0.494974675195202 2307 32-33 0.5263613046765485 2307 34-35 0.46437953903253515 2307 36-37 0.457536837397889 2307 38-39 0.4962698770024332 2307 40-41 0.5148877719895637 2307 42-43 0.5337046616321643 2307 44-45 0.4693067987614725 2307 46-47 0.4449173190080913 2307 48-49 0.5209176491892791 2307 50-51 0.49490886480365504 2307 52-53 0.4666024466427885 2307 54-55 0.39641970369483204 2307 56-57 0.48526893656051584 2307 58-59 0.5088989907745471 2307 60-61 0.4726680478904868 2307 62-63 0.512537121151837 2307 64-65 0.36909346553308353 2307 66-67 0.48232235542216273 2307 68-69 0.5532112757438199 2307 70-71 0.43557589892461834 2307 72-73 0.29520681862190656 2307 74-75 0.308272409971984 2307 76-77 0.3230261469314648 2307 78-79 0.3718593550515621 2307 80-81 0.4136477383653272 2307 82-83 0.36172179408114147 2307 84-85 0.410630408268716 2307 86-87 0.33561526499864414 2307 88-89 0.30814642442167894 2307 90-91 0.3739206787885152 2307 92-93 0.5162499812194454 2307 94-95 0.2996498669870178 2307 96-97 0.4530141577421034 2307 98-99 0.5055505899456207 2307 100-101 0.35164083201508234 2201 1 0.09256284667770842 2201 2 0.0983657235869444 2201 3 0.07893472271548063 2201 4 0.1589556070809195 2201 5 0.1148025232082901 2201 6 0.1449637188434849 2201 7 0.20157929489270288 2201 8 0.18261824389411885 2201 9 0.15132573347111844 2201 10-11 0.18182605039931587 2201 12-13 0.1918915209568297 2201 14-15 0.39028882766929485 2201 16-17 0.38626853541018136 2201 18-19 0.40417212010105885 2201 20-21 0.4137281458983253 2201 22-23 0.4367977102705112 2201 24-25 0.375113768180654 2201 26-27 0.43299489519613843 2201 28-29 0.46990516503199586 2201 30-31 0.4114610100110099 2201 32-33 0.44139437179952523 2201 34-35 0.47457924027241205 2201 36-37 0.4330918121741121 2201 38-39 0.5549521864458598 2201 40-41 0.4428879275200046 2201 42-43 0.4221147340471987 2201 44-45 0.527718841872705 2201 46-47 0.5694654998735658 2201 48-49 0.44082221210770456 2201 50-51 0.5854419679111516 2201 52-53 0.6172739042157502 2201 54-55 0.594440015390056 2201 56-57 0.6183241572712532 2201 58-59 0.5485281577545038 2201 60-61 0.5349015953545617 2201 62-63 0.6214390770839202 2201 64-65 0.5336477653780562 2201 66-67 0.5882531527507027 2201 68-69 0.6271855798940464 2201 70-71 0.4130288159494526 2201 72-73 0.4839595651589761 2201 74-75 0.5155292330648038 2201 76-77 0.33242625911946533 2201 78-79 0.4559109365755134 2201 80-81 0.5652459182406204 2201 82-83 0.5339308358413817 2201 84-85 0.6122863847671951 2201 86-87 0.6646062307090119 2201 88-89 0.5478826232288583 2201 90-91 0.6645011413965634 2201 92-93 0.7255563726348058 2201 94-95 0.6149329822906182 2201 96-97 0.6161042617075978 2201 98-99 0.7272941231559642 2201 100-101 0.6283536672850083 1101 1 0.048234417830997245 1101 2 0.06482076424816086 1101 3 0.0592260462664882 1101 4 0.10903899464948807 1101 5 0.08804029826923454 1101 6 0.10956744831555909 1101 7 0.13496409738522885 1101 8 0.10815798216895445 1101 9 0.14577505836200544 1101 10-11 0.14861902939195915 1101 12-13 0.12634307028858416 1101 14-15 0.32255453075915597 1101 16-17 0.26098599086994767 1101 18-19 0.25373883927959184 1101 20-21 0.24987316669886894 1101 22-23 0.2845775995292712 1101 24-25 0.30305368684373946 1101 26-27 0.24566522205281416 1101 28-29 0.23833247085467946 1101 30-31 0.3448335389772552 1101 32-33 0.43575336498514616 1101 34-35 0.40430374413955406 1101 36-37 0.3937193897956561 1101 38-39 0.3036897148777129 1101 40-41 0.3640619109461767 1101 42-43 0.3650994253756181 1101 44-45 0.31989498659806515 1101 46-47 0.3777675375251235 1101 48-49 0.3812226925637603 1101 50-51 0.38203049964405267 1101 52-53 0.32867453296786664 1101 54-55 0.34740847090689186 1101 56-57 0.3042854951565772 1101 58-59 0.3534957788163666 1101 60-61 0.3827517224923582 1101 62-63 0.41100432778390683 1101 64-65 0.40890130963935434 1101 66-67 0.34280616033668565 1101 68-69 0.40876398723493423 1101 70-71 0.3868497826370003 1101 72-73 0.23964800064425873 1101 74-75 0.3589525635683444 1101 76-77 0.09991422382268667 1101 78-79 0.3784999960338311 1101 80-81 0.4098441763991474 1101 82-83 0.34929699386994884 1101 84-85 0.364816867282606 1101 86-87 0.4594178889225162 1101 88-89 0.3111224634282763 1101 90-91 0.37538418214931113 1101 92-93 0.5476188797128998 1101 94-95 0.4498194054679061 1101 96-97 0.4713509081747205 1101 98-99 0.44956717732775786 1101 100-101 0.413519383785232 2304 1 0.07438900443125362 2304 2 0.059255834324289935 2304 3 0.08687578027002019 2304 4 0.11270737206412917 2304 5 0.11511499842308837 2304 6 0.13514957481033463 2304 7 0.11717299157207606 2304 8 0.13878672610060505 2304 9 0.07565676313889469 2304 10-11 0.13116018405676044 2304 12-13 0.10675133343293908 2304 14-15 0.18538138884903788 2304 16-17 0.18616229729755673 2304 18-19 0.2095339906226812 2304 20-21 0.1825035807925346 2304 22-23 0.2823192379787969 2304 24-25 0.20274413470888675 2304 26-27 0.26066604268125815 2304 28-29 0.1811100694602672 2304 30-31 0.17183541206909325 2304 32-33 0.09022186997831483 2304 34-35 0.1833804213249124 2304 36-37 0.18993195691346187 2304 38-39 0.26371580472475387 2304 40-41 0.20287509215934563 2304 42-43 0.17528480029194782 2304 44-45 0.2440715418869459 2304 46-47 0.2997713082583644 2304 48-49 0.30238247317780775 2304 50-51 0.2752778758603185 2304 52-53 0.2744245586558165 2304 54-55 0.22606291545626078 2304 56-57 0.25165153523531814 2304 58-59 0.19530069019344865 2304 60-61 0.17365425935282985 2304 62-63 0.22049370287287218 2304 64-65 0.14771537186496886 2304 66-67 0.19486699691838538 2304 68-69 0.2295702640823123 2304 70-71 0.24943451079120393 2304 72-73 0.24040280062770591 2304 74-75 0.23335404430277862 2304 76-77 0.11939375532996976 2304 78-79 0.3342290219488646 2304 80-81 0.38299358997153377 2304 82-83 0.43607008683932946 2304 84-85 0.36188319484856635 2304 86-87 0.2696518680082747 2304 88-89 0.2995978803104933 2304 90-91 0.2123244695019082 2304 92-93 0.477902930002287 2304 94-95 0.2775627148713582 2304 96-97 0.351988323644548 2304 98-99 0.40912400431295026 2304 100-101 0.4326585376212897 2202 1 0.09484628567386721 2202 2 0.09907089896498888 2202 3 0.07786720219746712 2202 4 0.09827419883347233 2202 5 0.10642792622847708 2202 6 0.10617969205457456 2202 7 0.08651089760500241 2202 8 0.10686862731795799 2202 9 0.07349339794981091 2202 10-11 0.13800265042252846 2202 12-13 0.11362386303305527 2202 14-15 0.17315153150554607 2202 16-17 0.08115615563858825 2202 18-19 0.1503195885133124 2202 20-21 0.13336699836735733 2202 22-23 0.16209174673802806 2202 24-25 0.08852762757926769 2202 26-27 0.08516419970849398 2202 28-29 0.17048046694610264 2202 30-31 0.10891019731145235 2202 32-33 0.2013749409518013 2202 34-35 0.23282161483264474 2202 36-37 0.25481117798084796 2202 38-39 0.19774360322512763 2202 40-41 0.2155261910885855 2202 42-43 0.20223539869283513 2202 44-45 0.23779589739132945 2202 46-47 0.1974116962139405 2202 48-49 0.24740562127440313 2202 50-51 0.15248782421657836 2202 52-53 0.23226751609895047 2202 54-55 0.23355308492709526 2202 56-57 0.26299025892883776 2202 58-59 0.21762131296945597 2202 60-61 0.09646277056475583 2202 62-63 0.20998494713773397 2202 64-65 0.022842732344763306 2202 66-67 0.1290404053952514 2202 68-69 0.18439426411009663 2202 70-71 0.057443911661088976 2202 72-73 0.18910804912802348 2202 74-75 0.19720414073227488 2202 76-77 0.2460163423174535 2202 78-79 0.3759298604918939 2202 80-81 0.37743720650172463 2202 82-83 0.32021524478354735 2202 84-85 0.3357350249988045 2202 86-87 0.21037765119709917 2202 88-89 0.310743041312989 2202 90-91 0.2646440432566166 2202 92-93 0.4105655755888762 2202 94-95 0.21835552793822188 2202 96-97 0.2470816207725406 2202 98-99 0.3316189968173333 2202 100-101 0.3179720196591056 1102 1 0.06148827856697636 1102 2 0.042671864258039705 1102 3 0.027665323463331504 1102 4 0.08794571136577645 1102 5 0.0439438257330238 1102 6 0.057699642361342285 1102 7 0.06726580396459525 1102 8 0.042289628208962426 1102 9 0.028742192127154453 1102 10-11 0.03580289708971662 1102 12-13 0.06724232307212219 1102 14-15 0.05196247872447657 1102 16-17 0.07894541021902057 1102 18-19 0.0450336910828355 1102 20-21 -0.06093171972516842 1102 22-23 0.01777152066348009 1102 24-25 0.02636762829884276 1102 26-27 -0.005850966461174778 1102 28-29 0.05232023009903486 1102 30-31 0.05062696549509127 1102 32-33 0.04903389102365452 1102 34-35 0.008375068384921747 1102 36-37 -0.013755038561406252 1102 38-39 -5.016179287764544E-4 1102 40-41 0.020734733857992182 1102 42-43 0.1370910676995365 1102 44-45 0.07296220197610381 1102 46-47 0.0502120196008633 1102 48-49 -0.04808118134317141 1102 50-51 -0.13870455132283865 1102 52-53 -0.07837057519859769 1102 54-55 -0.03660760588918066 1102 56-57 -0.010141932885318283 1102 58-59 -0.04655611121557257 1102 60-61 -0.1676758043316937 1102 62-63 -0.14267995268215827 1102 64-65 -0.13472621224558878 1102 66-67 -0.08188476322944638 1102 68-69 -0.007133707377676046 1102 70-71 -0.09303126130225081 1102 72-73 -0.18553865647346868 1102 74-75 -0.24274381418403124 1102 76-77 -0.22408334307870703 1102 78-79 0.006958841505607438 1102 80-81 0.0648489920293116 1102 82-83 6.841187917459024E-4 1102 84-85 0.03785121995371554 1102 86-87 0.01986857075459625 1102 88-89 -0.03807110961642479 1102 90-91 -0.07775432689866335 1102 92-93 -0.05162311422846244 1102 94-95 -0.23634412486527623 1102 96-97 -0.03353833821149976 1102 98-99 0.08983463589805041 1102 100-101 0.021053110836017908 2305 1 0.04085142734581382 2305 2 0.028034530666438684 2305 3 0.03397838187999014 2305 4 0.0393761795001808 2305 5 0.08818072133603039 2305 6 0.03361689843517013 2305 7 0.038781335728643285 2305 8 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88-89 -0.29305209220760275 1213 90-91 -0.24407985783265218 1213 92-93 -0.2343385070544599 1213 94-95 -0.19666733311968443 1213 96-97 -0.3117837003691122 1213 98-99 -0.09347934177215933 1213 100-101 0.0269792010171237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 12.0 10 3.0 11 18.0 12 52.0 13 172.0 14 539.0 15 1498.0 16 3272.0 17 5306.0 18 6778.0 19 8298.0 20 11202.0 21 14346.0 22 18748.0 23 23953.0 24 30089.0 25 38623.0 26 49685.0 27 65098.0 28 88864.0 29 119581.0 30 131824.0 31 154416.0 32 207202.0 33 296493.0 34 455292.0 35 767554.0 36 1428398.0 37 2279121.0 38 805149.0 39 381.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.00851508551308 27.679829241042565 20.142123853113347 16.16953182033101 2 30.700525159270548 24.626481467114512 30.165183298041292 14.50781007557365 3 35.650048602617694 20.998783793651082 25.463407705226267 17.887759898504957 4 29.622595268439493 21.377528268764458 30.069725767378593 18.930150695417456 5 30.448998055895295 19.733832213723737 31.541073775579125 18.276095954801846 6 27.561492009090742 20.120300035596284 33.70624338274219 18.611964572570784 7 32.00422477683868 21.220946815501726 26.30368535623665 20.47114305142294 8 29.214722599989045 27.656558044760043 23.486701593618225 19.642017761632683 9 27.93931746408426 24.8755556214746 26.655997289206113 20.529129625235026 10-11 22.720704087639877 29.29621897875176 30.88635030850112 17.096726625107245 12-13 22.088912841587412 28.21096017551706 30.81381717657582 18.88630980631971 14-15 21.62437849117395 28.389932754969788 31.344388622423835 18.64130013143243 16-17 21.85863512212263 27.784218353534985 31.98569217657582 18.371454347766562 18-19 20.91880624669137 27.821618695350576 32.532407449663204 18.72716760829485 20-21 21.603371549898686 28.183756685712197 31.9344939965499 18.278377767839217 22-23 21.06867857925193 29.681766944743615 30.72046250068454 18.52909197531991 24-25 21.054445770431354 28.16725632518574 31.404557179952903 19.373740724430004 26-27 21.105430030485024 28.298054126887056 30.68493752395904 19.911578318668884 28-29 21.656566316332302 29.104596598273126 30.42556668826783 18.81327039712674 30-31 20.858880131797523 28.702983242365054 30.99318194264435 19.444954683193078 32-33 20.73708836092806 29.37917714399153 30.394277326992935 19.489457168087476 34-35 21.514380841441376 29.22245224165313 30.531934829137843 18.73123208776766 36-37 21.41381706248517 29.072273290465674 30.72896938491448 18.78494026213468 38-39 21.089257680582683 28.530456784742885 31.506311780087987 18.873973754586444 40-41 20.996173969989595 28.67982284003578 29.928744683375623 20.395258506599003 42-43 20.815875086708896 29.148143573958123 30.758076762472392 19.277904576860593 44-45 20.681583258794106 28.442043660210658 30.932150574560524 19.944222506434713 46-47 21.380287099408633 28.510446042808418 30.1043224495742 20.004944408208743 48-49 20.833117920202323 29.476909411891132 30.2916737767856 19.398298891120945 50-51 21.52725486169345 28.823702645194782 30.051790084370133 19.597252408741635 52-53 20.77420815973568 28.774462303349786 29.486841685856387 20.96448785105815 54-55 21.369675644798857 28.935239495699438 29.995749715280844 19.69933514422086 56-57 20.687750277243893 28.842350742942312 30.212630910821964 20.25726806899183 58-59 20.73809340253265 28.907272339697492 30.30799439534312 20.04663986242674 60-61 21.159272648754786 28.989574662577226 30.260661602713345 19.590491085954643 62-63 20.32059539654418 28.897540181387154 30.272109255864848 20.509755166203817 64-65 21.04060502292141 28.74757434082438 30.197960032310984 20.013860603943225 66-67 20.56195189025984 29.747733567450236 29.635833864957956 20.054480677331966 68-69 20.465802920075067 30.02172620981452 29.532267747416206 19.980203122694206 70-71 20.266686256371706 30.298274655747782 29.397231432632694 20.037807655247818 72-73 20.274355106316456 30.191913353857952 29.38552523774856 20.148206302077035 74-75 20.410995043898396 30.03435086437753 29.394128008482195 20.16052608324188 76-77 20.450763991655823 29.86824754399415 29.419380605131312 20.261607859218714 78-79 20.316898699112677 29.80500488947881 29.65994286373205 20.218153547676465 80-81 20.221389362579988 30.018851297378003 29.536601509558498 20.22315783048351 82-83 20.1063232551833 29.8831838961049 29.513556097379055 20.496936751332747 84-85 20.218265282816215 29.612573168742895 29.682541052115308 20.486620496325582 86-87 20.330447853034634 29.622054333219843 29.73840971328865 20.309088100456876 88-89 20.526200780556213 29.851069007137177 29.387433678253483 20.235296534053127 90-91 20.59828865376031 29.727346685866895 29.300134173870024 20.37423048650277 92-93 20.30909145501408 29.870588912415812 29.267998009027274 20.55232162354283 94-95 20.062404276297606 29.935131646278297 29.21632709825464 20.786136979169452 96-97 20.134811628738607 30.201158332164862 29.072524066067412 20.591505973029115 98-99 20.37734086070207 30.161576895780655 28.949233057179235 20.51184918633804 100-101 20.480793149800196 29.9906630197386 28.65555757148367 20.872986258977537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 205.0 1 117.5 2 33.5 3 40.0 4 41.0 5 39.0 6 50.0 7 61.0 8 65.5 9 76.0 10 89.5 11 106.0 12 122.5 13 143.5 14 166.0 15 193.0 16 244.5 17 295.5 18 349.5 19 502.0 20 771.0 21 1235.5 22 2122.5 23 3687.0 24 6099.0 25 10080.5 26 17174.5 27 27285.0 28 39243.0 29 53390.0 30 70417.5 31 89631.5 32 115246.5 33 150971.0 34 194373.5 35 242534.0 36 297723.5 37 359682.5 38 416533.0 39 450823.0 40 469439.5 41 464407.0 42 437344.5 43 414993.0 44 384591.0 45 352697.5 46 333513.5 47 304673.0 48 261752.5 49 221515.5 50 181736.0 51 145464.0 52 117012.0 53 93168.5 54 75788.0 55 59621.0 56 42431.5 57 28896.0 58 20425.0 59 15112.0 60 10891.5 61 7631.0 62 5300.5 63 3545.5 64 2388.0 65 1656.5 66 1144.0 67 796.0 68 585.5 69 414.0 70 301.0 71 228.0 72 166.0 73 118.0 74 89.5 75 62.5 76 50.5 77 42.5 78 34.0 79 26.5 80 14.5 81 8.0 82 5.5 83 6.0 84 4.5 85 2.5 86 1.5 87 2.0 88 2.0 89 1.0 90 1.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19345496157426845 2 0.0 3 0.0 4 0.027581415089173254 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.269865464303317E-5 48-49 0.0035296795421770324 50-51 1.1409065186834851E-4 52-53 0.0026312156587137876 54-55 0.00959787608842482 56-57 0.01416150216315876 58-59 0.014439598127087859 60-61 0.00360098619959475 62-63 0.002431557017944178 64-65 0.01040364131724503 66-67 0.01747726173308264 68-69 0.025263948723097424 70-71 0.021149554590095106 72-73 0.017113597780252277 74-75 0.021655831857760904 76-77 0.015587635311513115 78-79 0.006909615103776857 80-81 0.0035510715394023477 82-83 7.059359084354065E-4 84-85 0.00255990900129607 86-87 0.015958429930085248 88-89 0.015259624687391612 90-91 0.018375725616545883 92-93 0.018889133549953453 94-95 0.017548568390500355 96-97 0.019552285463938227 98-99 0.02842283364670232 100-101 0.016200872565305488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 7011968.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.62940474218624 #Duplication Level Percentage of deduplicated Percentage of total 1 91.75230929613105 60.216494428260404 2 4.467159700585902 5.863540640754713 3 1.3182540041583208 2.5954867677574227 4 0.5889636756823411 1.546133417992083 5 0.3278046592582518 1.0756812329417127 6 0.2487627936375323 0.9795692441060735 7 0.17910966268333603 0.8228402381836777 8 0.11991622153210257 0.6296024190467222 9 0.12716574631961103 0.7511231011096738 >10 0.7185161655966976 9.566257233789527 >50 0.08851869477745809 4.141068367421346 >100 0.05715131586259761 7.563536633903882 >500 0.005537284034254115 2.455110868013034 >1k 8.090611980753885E-4 0.7220867462559701 >5k 0.0 0.0 >10k+ 2.1718542643490317E-5 1.0714686604637507 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT 75171 1.0720385489494533 TruSeq Adapter, Index 7 (97% over 38bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 8.414185575290702E-4 0.0 0.0 2 9.555092093974188E-4 0.0 0.0 3 0.0010268158668151365 0.0 0.0 4 0.0010695998612657673 0.0 0.0 5 0.001212213176101203 0.0 1.4261331483543564E-5 6 0.0015259624687391615 0.0 1.4261331483543564E-5 7 0.0019538024132454684 0.0 7.130665741771782E-5 8 0.002324597031817601 0.0 7.130665741771782E-5 9 0.002709652981873277 0.0 8.556798890126139E-5 10-11 0.004870244701630127 0.0 8.556798890126139E-5 12-13 0.007266148390865446 0.0 8.556798890126139E-5 14-15 0.010966963910845 0.0 8.556798890126139E-5 16-17 0.016029736587502966 0.0 8.556798890126139E-5 18-19 0.02168435452072799 0.0 9.269865464303317E-5 20-21 0.024550882148920247 0.0 9.982932038480494E-5 22-23 0.026996700498347966 0.0 9.982932038480494E-5 24-25 0.029706353480221243 0.0 9.982932038480494E-5 26-27 0.03258714243989705 0.0 9.982932038480494E-5 28-29 0.03608116865336522 0.0 9.982932038480494E-5 30-31 0.05465655291068071 0.0 9.982932038480494E-5 32-33 0.059983160219784226 0.0 1.0695998612657672E-4 34-35 0.06419025300742959 0.0 1.1409065186834851E-4 36-37 0.0688608390682901 0.0 1.1409065186834851E-4 38-39 0.07413040105145945 0.0 1.1409065186834851E-4 40-41 0.07994902429674522 0.0 1.2835198335189207E-4 42-43 0.08605287417170188 0.0 1.2835198335189207E-4 44-45 0.09289831328380277 0.0 1.2835198335189207E-4 46-47 0.0999434110366733 0.0 1.4261331483543565E-4 48-49 0.10772296736094632 0.0 1.568746463189792E-4 50-51 0.11635820357423193 0.0 1.568746463189792E-4 52-53 0.12562093837279348 0.0 1.568746463189792E-4 54-55 0.13562526240849931 0.0 1.7826664354429457E-4 56-57 0.14660648765082784 0.0 1.8539730928606634E-4 58-59 0.15883557939796644 0.0 1.996586407696099E-4 60-61 0.17265480960552015 0.0 1.996586407696099E-4 62-63 0.1866665677881017 0.0 2.3531196947846881E-4 64-65 0.2032738883006882 0.0 2.424426352202406E-4 66-67 0.22087950201712273 0.0 2.424426352202406E-4 68-69 0.2404460488125445 0.0 2.424426352202406E-4 70-71 0.26254398194629525 0.0 2.4957330096201237E-4 72-73 0.28771523201474963 0.0 2.7809596392909953E-4 74-75 0.3170293988791735 0.0 2.994879611544148E-4 76-77 0.3501299492524781 0.0 3.137492926379584E-4 78-79 0.3865106058669977 0.0 3.28010624121502E-4 80-81 0.4293088616491119 0.0 3.28010624121502E-4 82-83 0.47627142622442087 0.0 3.565332870885891E-4 84-85 0.5301436059035067 0.0 3.565332870885891E-4 86-87 0.592622499132911 0.0 3.8505595005567625E-4 88-89 0.6625885913911758 0.0 3.92186615797448E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGAAGA 15365 0.0 54.970047 4 TCGGAAG 15460 0.0 54.755177 3 GATCGGA 16080 0.0 52.630905 1 ATCGGAA 16350 0.0 51.77462 2 GAAGAGC 18230 0.0 46.320137 6 CGACGGG 140 0.0 44.1988 1 GCCGTCT 10000 0.0 42.20323 50-51 TATGCCG 9975 0.0 42.04545 48-49 TGCCGTC 10055 0.0 41.736183 50-51 ATGCCGT 10130 0.0 41.495884 48-49 CGTATGC 10105 0.0 41.45591 46-47 TCGTATG 10355 0.0 40.684235 44-45 TCTCGTA 10265 0.0 40.531975 42-43 CTCGTAT 10250 0.0 40.498615 44-45 CCGTCTT 10485 0.0 40.09478 52-53 CATCTCG 10370 0.0 39.867115 40-41 CGGACGG 120 8.54925E-11 39.665585 1 ATCTCGT 10540 0.0 39.159016 42-43 CGTCTTC 10820 0.0 39.00706 52-53 GTATGCC 10835 0.0 38.903942 46-47 >>END_MODULE