FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_35010-1.341000000357eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_35010-1.341000000357eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011968
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT751711.0720385489494533TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA153650.054.9700474
TCGGAAG154600.054.7551773
GATCGGA160800.052.6309051
ATCGGAA163500.051.774622
GAAGAGC182300.046.3201376
CGACGGG1400.044.19881
GCCGTCT100000.042.2032350-51
TATGCCG99750.042.0454548-49
TGCCGTC100550.041.73618350-51
ATGCCGT101300.041.49588448-49
CGTATGC101050.041.4559146-47
TCGTATG103550.040.68423544-45
TCTCGTA102650.040.53197542-43
CTCGTAT102500.040.49861544-45
CCGTCTT104850.040.0947852-53
CATCTCG103700.039.86711540-41
CGGACGG1208.54925E-1139.6655851
ATCTCGT105400.039.15901642-43
CGTCTTC108200.039.0070652-53
GTATGCC108350.038.90394246-47
Produced by FastQC (version 0.11.2)