##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C68Y0ACXX_l08n01_35008-2.341000000358b3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5937612 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.6884109301854 33.0 33.0 33.0 33.0 33.0 2 32.71902997366618 33.0 33.0 33.0 33.0 33.0 3 32.752815610046596 33.0 33.0 33.0 33.0 33.0 4 36.68176718182327 37.0 37.0 37.0 37.0 37.0 5 36.67386097306459 37.0 37.0 37.0 37.0 37.0 6 36.547527692951306 37.0 37.0 37.0 37.0 37.0 7 36.465930579498966 37.0 37.0 37.0 37.0 37.0 8 36.40249783919865 37.0 37.0 37.0 37.0 37.0 9 36.44073425478121 37.0 37.0 37.0 37.0 37.0 10-11 36.37910072264742 37.0 37.0 37.0 37.0 37.0 12-13 36.353494553029066 37.0 37.0 37.0 37.0 37.0 14-15 38.193069284419394 40.0 37.0 40.0 37.0 40.0 16-17 38.187451200920506 40.0 37.0 40.0 37.0 40.0 18-19 38.15816771119434 40.0 37.0 40.0 37.0 40.0 20-21 38.12077111134914 40.0 37.0 40.0 37.0 40.0 22-23 37.97487769493864 40.0 37.0 40.0 33.0 40.0 24-25 37.969533964159325 40.0 37.0 40.0 33.0 40.0 26-27 37.89676801043922 40.0 37.0 40.0 33.0 40.0 28-29 37.723161348366986 40.0 37.0 40.0 33.0 40.0 30-31 37.694569635065406 40.0 37.0 40.0 33.0 40.0 32-33 37.564410153442154 40.0 37.0 40.0 33.0 40.0 34-35 37.420720737562505 40.0 37.0 40.0 33.0 40.0 36-37 37.22101326257088 40.0 37.0 40.0 33.0 40.0 38-39 37.27090571091543 40.0 37.0 40.0 33.0 40.0 40-41 37.110954707043845 40.0 37.0 40.0 33.0 40.0 42-43 36.95791548858362 38.5 37.0 40.0 33.0 40.0 44-45 36.906430649223964 37.0 37.0 40.0 33.0 40.0 46-47 36.87682910233946 37.0 37.0 40.0 33.0 40.0 48-49 36.63137503764139 37.0 37.0 40.0 33.0 40.0 50-51 36.53586719711561 37.0 37.0 40.0 33.0 40.0 52-53 36.43235437074703 37.0 37.0 40.0 33.0 40.0 54-55 36.19635663293593 37.0 37.0 40.0 33.0 40.0 56-57 36.06061965652185 37.0 37.0 40.0 30.0 40.0 58-59 35.81774415371028 37.0 37.0 40.0 27.0 40.0 60-61 35.599346589167496 37.0 35.0 40.0 27.0 40.0 62-63 35.37952993560374 37.0 33.0 40.0 27.0 40.0 64-65 35.16841071797887 37.0 33.0 40.0 27.0 40.0 66-67 34.743143624069745 37.0 33.0 40.0 27.0 40.0 68-69 34.530133578953965 37.0 33.0 37.0 27.0 40.0 70-71 34.15457645262102 37.0 33.0 37.0 27.0 40.0 72-73 33.61636117348186 37.0 33.0 37.0 24.5 40.0 74-75 33.08487023065838 37.0 33.0 37.0 22.0 40.0 76-77 30.592322890077696 33.0 30.0 35.0 22.0 37.0 78-79 32.69482226187902 37.0 33.0 37.0 22.0 37.0 80-81 32.95209176685846 37.0 33.0 37.0 22.0 37.0 82-83 32.7833515393057 37.0 33.0 37.0 22.0 37.0 84-85 32.54924067116544 37.0 33.0 37.0 22.0 37.0 86-87 32.39619833697453 37.0 33.0 37.0 22.0 37.0 88-89 32.208592191608346 37.0 33.0 37.0 22.0 37.0 90-91 32.027270138230655 33.0 33.0 37.0 22.0 37.0 92-93 31.875243616457254 33.0 33.0 37.0 22.0 37.0 94-95 31.724092951846636 33.0 33.0 37.0 18.5 37.0 96-97 31.54486854311127 33.0 33.0 37.0 15.0 37.0 98-99 31.36400627053435 33.0 33.0 37.0 15.0 37.0 100-101 29.68035836629271 33.0 30.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.11143498198929791 2306 2 0.09589360997618712 2306 3 0.11217793244809826 2306 4 0.17955130590019763 2306 5 0.1481374548556289 2306 6 0.21960306955922704 2306 7 0.2134333717325987 2306 8 0.2508522004357019 2306 9 0.2259409806510959 2306 10-11 0.24388019879761913 2306 12-13 0.20200825207447082 2306 14-15 0.42749591688289 2306 16-17 0.44092454794740377 2306 18-19 0.37156077011670874 2306 20-21 0.408120013880513 2306 22-23 0.4864616677466955 2306 24-25 0.3393201866952822 2306 26-27 0.4578282507228195 2306 28-29 0.4173595943499677 2306 30-31 0.4079108189917946 2306 32-33 0.5208375573418138 2306 34-35 0.5122371948321174 2306 36-37 0.5193371212516311 2306 38-39 0.5327746271215972 2306 40-41 0.5922672110712384 2306 42-43 0.6764364101231308 2306 44-45 0.641827205932394 2306 46-47 0.6062671835309743 2306 48-49 0.667457332703357 2306 50-51 0.6724859330920552 2306 52-53 0.6685295780356739 2306 54-55 0.6651066618785393 2306 56-57 0.63850010674971 2306 58-59 0.5831710872696547 2306 60-61 0.6835079066532614 2306 62-63 0.6422567869501279 2306 64-65 0.6987740296526752 2306 66-67 0.6371834623428114 2306 68-69 0.7228787694825201 2306 70-71 0.5929782320328698 2306 72-73 0.5269199695834317 2306 74-75 0.5422033961805113 2306 76-77 0.5714501313468006 2306 78-79 0.7409492206175301 2306 80-81 0.6738578953118477 2306 82-83 0.5731299594109487 2306 84-85 0.6325230841378513 2306 86-87 0.6939806253468248 2306 88-89 0.4950080545933133 2306 90-91 0.5042333006837225 2306 92-93 0.7458285899170392 2306 94-95 0.6761198159246185 2306 96-97 0.711671795222653 2306 98-99 0.8483481682122971 2306 100-101 0.5834568844208867 2307 1 0.11607989073012703 2307 2 0.08841717856281406 2307 3 0.11595494214989088 2307 4 0.14908062554570733 2307 5 0.1921797233001996 2307 6 0.2063230125808886 2307 7 0.17121134826634687 2307 8 0.20507155496288476 2307 9 0.17756278364454658 2307 10-11 0.20561831793084906 2307 12-13 0.18345800068170348 2307 14-15 0.4551133729735142 2307 16-17 0.407458416970762 2307 18-19 0.4132532582933166 2307 20-21 0.4346590847012308 2307 22-23 0.486091423783499 2307 24-25 0.47759161516481186 2307 26-27 0.37607794467826494 2307 28-29 0.421870342810692 2307 30-31 0.45659799617719443 2307 32-33 0.5547195851867954 2307 34-35 0.5363722176887507 2307 36-37 0.48718850458403296 2307 38-39 0.5334240012872016 2307 40-41 0.5164073918282028 2307 42-43 0.5113337761370715 2307 44-45 0.42487129445732563 2307 46-47 0.5296740370636215 2307 48-49 0.5235640123489134 2307 50-51 0.5657127045701884 2307 52-53 0.5653365908047618 2307 54-55 0.39156913239208535 2307 56-57 0.5243589822067207 2307 58-59 0.4597915596345601 2307 60-61 0.48065366727071535 2307 62-63 0.4866187903444654 2307 64-65 0.38524027531030214 2307 66-67 0.5680207651560139 2307 68-69 0.6551291175036198 2307 70-71 0.48982659031160125 2307 72-73 0.29507232188777976 2307 74-75 0.36751897463080496 2307 76-77 0.370892309912211 2307 78-79 0.40374739801308834 2307 80-81 0.3865711868369246 2307 82-83 0.4181683885120435 2307 84-85 0.46552634843030916 2307 86-87 0.46467401176629153 2307 88-89 0.37396832065316943 2307 90-91 0.3891620256342918 2307 92-93 0.5983651520228221 2307 94-95 0.4518247609792603 2307 96-97 0.5489278306519445 2307 98-99 0.5909122043680739 2307 100-101 0.36124520967808493 2201 1 0.08356606690108492 2201 2 0.09092247756520777 2201 3 0.08928768514298469 2201 4 0.14601947962372464 2201 5 0.12187188779121527 2201 6 0.13587565295090087 2201 7 0.20274247032556758 2201 8 0.17305575402234297 2201 9 0.14415537147291246 2201 10-11 0.20883808370825108 2201 12-13 0.21082479553604827 2201 14-15 0.41545536709581654 2201 16-17 0.4206283129723758 2201 18-19 0.3626994359817104 2201 20-21 0.4182900524505655 2201 22-23 0.4831143553320132 2201 24-25 0.38200764817859323 2201 26-27 0.41210384984418624 2201 28-29 0.4361172433454783 2201 30-31 0.43310272726681376 2201 32-33 0.37733595950040666 2201 34-35 0.39336899191560804 2201 36-37 0.40666470679983746 2201 38-39 0.39644809995064634 2201 40-41 0.47436037409111265 2201 42-43 0.42040193395137493 2201 44-45 0.4938603995100408 2201 46-47 0.5252127162277702 2201 48-49 0.4901046301263392 2201 50-51 0.4909353168646078 2201 52-53 0.5204055036547004 2201 54-55 0.47299311383277143 2201 56-57 0.6231039450996505 2201 58-59 0.614976918119531 2201 60-61 0.49866301543795544 2201 62-63 0.4802780351186513 2201 64-65 0.5517492764822265 2201 66-67 0.6343349335978701 2201 68-69 0.5802363884946331 2201 70-71 0.41240645010443444 2201 72-73 0.4106882510943919 2201 74-75 0.37779662098516553 2201 76-77 0.237382450531463 2201 78-79 0.4281607192792123 2201 80-81 0.39784994048824984 2201 82-83 0.4059997531867836 2201 84-85 0.4621869722897358 2201 86-87 0.47313030339410034 2201 88-89 0.4440518132369249 2201 90-91 0.5015787419021507 2201 92-93 0.556849440502468 2201 94-95 0.435399745250205 2201 96-97 0.46184680819218826 2201 98-99 0.4955043424880614 2201 100-101 0.44505027837620403 1101 1 0.10336402274429446 1101 2 0.0875378683237571 1101 3 0.08508842621563417 1101 4 0.12803093166349555 1101 5 0.1336387434019315 1101 6 0.13191934413362816 1101 7 0.10778717786828906 1101 8 0.16623730026886818 1101 9 0.14064942744784759 1101 10-11 0.17962513312077988 1101 12-13 0.20756271338533594 1101 14-15 0.360172527766494 1101 16-17 0.2799004202283939 1101 18-19 0.3495526602162897 1101 20-21 0.35555823930724983 1101 22-23 0.3220975567533202 1101 24-25 0.3469744854418124 1101 26-27 0.2847177569051098 1101 28-29 0.2997056192768355 1101 30-31 0.3264679682973153 1101 32-33 0.3741961706547272 1101 34-35 0.39097724720699034 1101 36-37 0.38185539060761187 1101 38-39 0.42873668858491243 1101 40-41 0.4623521113559903 1101 42-43 0.4041988308388298 1101 44-45 0.3813165898213313 1101 46-47 0.44951280973738506 1101 48-49 0.5125512845522948 1101 50-51 0.49758434611386804 1101 52-53 0.43827083251848364 1101 54-55 0.47199814522855377 1101 56-57 0.520435267190102 1101 58-59 0.3957412823694497 1101 60-61 0.4385253586711926 1101 62-63 0.5383110827861088 1101 64-65 0.46114298269971243 1101 66-67 0.5625028012762883 1101 68-69 0.5797463035102552 1101 70-71 0.5619367488961231 1101 72-73 0.3678032316291606 1101 74-75 0.4481881423816816 1101 76-77 0.22061940726431928 1101 78-79 0.49585521858666937 1101 80-81 0.527102344752187 1101 82-83 0.43150252455272664 1101 84-85 0.5912428329070778 1101 86-87 0.6454126041279054 1101 88-89 0.489878207615547 1101 90-91 0.6003483417362929 1101 92-93 0.7202493337185132 1101 94-95 0.6142071921854075 1101 96-97 0.703151908503326 1101 98-99 0.7866042560948898 1101 100-101 0.6272000894750107 2304 1 0.050833221343452806 2304 2 0.03618590131932109 2304 3 0.07650758585331374 2304 4 0.09340816162101362 2304 5 0.0900942885814402 2304 6 0.11696628126760089 2304 7 0.0857893507409031 2304 8 0.14198583650523489 2304 9 0.09833510651100852 2304 10-11 0.1294161288088418 2304 12-13 0.15351322375411058 2304 14-15 0.2063975725892675 2304 16-17 0.27344751800521294 2304 18-19 0.2622530095839224 2304 20-21 0.2832927317083076 2304 22-23 0.2786346036243543 2304 24-25 0.2775763957723356 2304 26-27 0.3277231877377673 2304 28-29 0.34258067269448134 2304 30-31 0.2339322923913727 2304 32-33 0.18181432576667333 2304 34-35 0.23077967631569152 2304 36-37 0.30728380877000916 2304 38-39 0.2669328999765668 2304 40-41 0.2805909253489389 2304 42-43 0.25949657146103533 2304 44-45 0.3280358126792109 2304 46-47 0.3051708059617084 2304 48-49 0.2975213272535413 2304 50-51 0.3468690871003517 2304 52-53 0.2847312774945365 2304 54-55 0.2608493924805373 2304 56-57 0.32447415548931957 2304 58-59 0.26982814517354115 2304 60-61 0.3300903192919833 2304 62-63 0.36326197311674235 2304 64-65 0.29916350283183846 2304 66-67 0.3426134505187193 2304 68-69 0.40072392841902627 2304 70-71 0.4271455546701972 2304 72-73 0.2585257762658273 2304 74-75 0.37119711738925787 2304 76-77 0.18199144134673872 2304 78-79 0.3721915870904837 2304 80-81 0.3964983605229193 2304 82-83 0.483785448685893 2304 84-85 0.5188713330643466 2304 86-87 0.42974854807261664 2304 88-89 0.368067280074456 2304 90-91 0.4809080448551626 2304 92-93 0.5777142985292443 2304 94-95 0.4470235542685721 2304 96-97 0.48183487657615487 2304 98-99 0.5676536817387472 2304 100-101 0.45924565252461136 2202 1 0.08802970241450936 2202 2 0.06384882361711419 2202 3 0.07067923850466684 2202 4 0.11100933892693376 2202 5 0.10889490626821186 2202 6 0.12358354613400735 2202 7 0.14089958105775224 2202 8 0.14111352317413406 2202 9 0.09000526678607201 2202 10-11 0.14875352825156085 2202 12-13 0.12045010737291051 2202 14-15 0.10592941760339158 2202 16-17 0.059816172426181424 2202 18-19 0.09718001284266364 2202 20-21 0.13893003273403082 2202 22-23 0.14545697176441763 2202 24-25 0.13176024873837378 2202 26-27 0.09197477965037848 2202 28-29 0.0801188244644635 2202 30-31 0.06471221630290813 2202 32-33 0.13998225230989902 2202 34-35 0.13622538472435508 2202 36-37 0.21418802705478157 2202 38-39 0.16865803960056525 2202 40-41 0.2322630133165049 2202 42-43 0.2887525906484143 2202 44-45 0.1659765068334096 2202 46-47 0.15653008895483822 2202 48-49 0.2582148136775899 2202 50-51 0.17981640440258673 2202 52-53 0.20009084945053246 2202 54-55 0.2051725252413945 2202 56-57 0.2813259142546869 2202 58-59 0.20835870712226523 2202 60-61 0.11392598346762384 2202 62-63 0.18712000631874304 2202 64-65 0.15723790610861954 2202 66-67 0.22385920671091952 2202 68-69 0.2503759275843862 2202 70-71 0.16165954809037686 2202 72-73 0.1542946287432514 2202 74-75 0.31902310352469954 2202 76-77 0.2500948387080406 2202 78-79 0.3429110228687122 2202 80-81 0.29965305261281827 2202 82-83 0.28284896680340665 2202 84-85 0.27842498629377843 2202 86-87 0.2321852415581276 2202 88-89 0.2951643447786836 2202 90-91 0.22147856848842196 2202 92-93 0.31020259121638816 2202 94-95 0.07027370342979466 2202 96-97 0.19178817262854153 2202 98-99 0.34614678013350897 2202 100-101 0.43507603300434994 1102 1 0.07198380820280192 1102 2 0.04786919062390638 1102 3 0.05310581173456086 1102 4 0.08397620683737728 1102 5 0.10319806276233834 1102 6 0.085120530079827 1102 7 0.09101662022172974 1102 8 0.11431657003414131 1102 9 0.05274757049267009 1102 10-11 0.11534495671443068 1102 12-13 0.050539041206640434 1102 14-15 0.11873697098755542 1102 16-17 0.02454141358705897 1102 18-19 0.05134314528397255 1102 20-21 0.09455773195349337 1102 22-23 0.10632559853661405 1102 24-25 0.07075208218417828 1102 26-27 0.12770596549992064 1102 28-29 0.12652125619479193 1102 30-31 0.11028766925649336 1102 32-33 0.15431117031989317 1102 34-35 0.12562186274321618 1102 36-37 0.09858875812749801 1102 38-39 0.09288981654951556 1102 40-41 0.03580556715215977 1102 42-43 0.08778139855904499 1102 44-45 0.13295829493750233 1102 46-47 0.16255919017709886 1102 48-49 0.13626430122108246 1102 50-51 0.08400645370164028 1102 52-53 0.15536072046742788 1102 54-55 0.13025833175709067 1102 56-57 0.15329593500079142 1102 58-59 0.17680365219136007 1102 60-61 -0.0851374670768692 1102 62-63 0.17149680933760436 1102 64-65 0.13471723348958164 1102 66-67 0.08045955012730843 1102 68-69 0.07698267225742228 1102 70-71 -0.0678391956603619 1102 72-73 -0.03139690258663563 1102 74-75 -0.07972885585125766 1102 76-77 -0.06797358403599674 1102 78-79 0.11713919069597267 1102 80-81 0.1516845440323067 1102 82-83 0.167159980891185 1102 84-85 0.1701910395013968 1102 86-87 0.057044558065193485 1102 88-89 -0.013898614445928104 1102 90-91 0.007933851999382568 1102 92-93 -0.03410849900129165 1102 94-95 -0.18017747202659606 1102 96-97 -0.01043392905465268 1102 98-99 -0.005570428687384776 1102 100-101 0.16263372837432044 2305 1 0.050724500714835585 2305 2 0.06858929423498239 2305 3 0.060894873693811746 2305 4 0.08936095301621805 2305 5 0.07072287963836033 2305 6 -0.0045941287811217535 2305 7 -0.019859088071086717 2305 8 0.05419684105749667 2305 9 0.05719585854435394 2305 10-11 0.056398023357317584 2305 12-13 -3.6186234527946226E-4 2305 14-15 0.012998828451195266 2305 16-17 -0.0262255365993056 2305 18-19 -0.055191360885501695 2305 20-21 -0.06647071771988777 2305 22-23 0.023687086982405958 2305 24-25 0.11271841140008831 2305 26-27 0.05597237098097452 2305 28-29 -0.007802720063097013 2305 30-31 0.042087046776359216 2305 32-33 -0.042989183191615155 2305 34-35 0.08906580377148998 2305 36-37 -0.003976659924070702 2305 38-39 0.028240781403965798 2305 40-41 0.03892663579041056 2305 42-43 -0.018567946188937867 2305 44-45 0.022224792151668282 2305 46-47 0.06878428582197671 2305 48-49 0.1322896157204312 2305 50-51 0.09538925928387698 2305 52-53 0.04522479426434245 2305 54-55 0.14034318424141645 2305 56-57 0.10806258938946911 2305 58-59 0.014508530793399643 2305 60-61 -0.007300646328857852 2305 62-63 0.021395954095901004 2305 64-65 -0.004952051387519418 2305 66-67 0.002036443517191344 2305 68-69 0.07611162354585588 2305 70-71 -0.06957729495294274 2305 72-73 -0.12759307479564796 2305 74-75 -0.04792884502751349 2305 76-77 -0.0037354607546369323 2305 78-79 0.08365535919984524 2305 80-81 0.08291842596884891 2305 82-83 0.06182820834271752 2305 84-85 0.09356923463140987 2305 86-87 0.04980663040436184 2305 88-89 -0.02130871760025599 2305 90-91 0.060994065892330696 2305 92-93 0.10960411128154135 2305 94-95 0.021850526913546275 2305 96-97 0.19578993196751426 2305 98-99 0.22628519133438374 2305 100-101 0.1773037329149183 2203 1 -5.329687889741308E-4 2203 2 0.02679537235088958 2203 3 0.005456296135996297 2203 4 0.01590509339875723 2203 5 0.03759257692038176 2203 6 0.027959308011013206 2203 7 0.019567285927067246 2203 8 0.06830720691932868 2203 9 -0.025442192953342158 2203 10-11 0.0072576258033905106 2203 12-13 -0.01849607746790838 2203 14-15 -0.027851887313183 2203 16-17 -0.15162209220421374 2203 18-19 -0.05445515632936093 2203 20-21 -0.08704255968370234 2203 22-23 -0.06644796839441369 2203 24-25 -0.03278525812580568 2203 26-27 -0.05515560671022968 2203 28-29 -0.04663492504629119 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-0.7194734292920941 1216 18-19 -0.6759863963974979 1216 20-21 -0.7092174763543184 1216 22-23 -0.822874775253517 1216 24-25 -0.8269891896899182 1216 26-27 -0.9100096014102093 1216 28-29 -0.9286138649926983 1216 30-31 -0.9672450063053759 1216 32-33 -0.9277550963198138 1216 34-35 -0.965463994119311 1216 36-37 -1.1005630825316146 1216 38-39 -1.1361960077144673 1216 40-41 -1.117039056843275 1216 42-43 -1.0261497226619127 1216 44-45 -1.0306263265419418 1216 46-47 -1.0944814051128589 1216 48-49 -1.087996080724487 1216 50-51 -1.137318645002047 1216 52-53 -1.1006434202241877 1216 54-55 -1.2533009673317537 1216 56-57 -1.3837400079842652 1216 58-59 -1.3109093223126607 1216 60-61 -1.3264407910203033 1216 62-63 -1.2124417500574936 1216 64-65 -1.1051263264102076 1216 66-67 -1.2967474229807792 1216 68-69 -1.4310368223462433 1216 70-71 -1.48959021072519 1216 72-73 -1.253871825289643 1216 74-75 -1.1758671114802013 1216 76-77 -1.1352393762266964 1216 78-79 -1.0575915315846345 1216 80-81 -1.0574660279922554 1216 82-83 -1.122567953343733 1216 84-85 -1.084654075600227 1216 86-87 -1.0868474139544233 1216 88-89 -1.0560803626204702 1216 90-91 -1.0178535514510259 1216 92-93 -0.8804823685538352 1216 94-95 -1.2288568732755856 1216 96-97 -1.3364147158431336 1216 98-99 -1.214085425925937 1216 100-101 -1.2501815022823557 2308 1 -0.1600547235799965 2308 2 -0.21167720220302044 2308 3 -0.1765149053379318 2308 4 -0.2224620822304999 2308 5 -0.22654950099781246 2308 6 -0.37318541322665766 2308 7 -0.4463082289254956 2308 8 -0.43943604901744493 2308 9 -0.49526425373257155 2308 10-11 -0.48556850522815154 2308 12-13 -0.539482458735705 2308 14-15 -0.969343132365978 2308 16-17 -0.9365495468751277 2308 18-19 -0.9168415515552866 2308 20-21 -0.9154373193817023 2308 22-23 -1.022456436881356 2308 24-25 -0.9847717070032687 2308 26-27 -0.956628761146959 2308 28-29 -1.2085925043488075 2308 30-31 -1.0799566576003983 2308 32-33 -1.253459691638973 2308 34-35 -1.0914591253420483 2308 36-37 -1.1547566525919422 2308 38-39 -1.0582529762537973 2308 40-41 -1.1734485916001276 2308 42-43 -1.1749094815802152 2308 44-45 -1.078106600977769 2308 46-47 -1.0458263167552388 2308 48-49 -1.123879857898551 2308 50-51 -1.1690450023347765 2308 52-53 -1.220571153842613 2308 54-55 -1.2352701311693295 2308 56-57 -1.2165221950661476 2308 58-59 -1.19100909833886 2308 60-61 -1.3534131378923888 2308 62-63 -1.2159437989060606 2308 64-65 -1.1648012840880924 2308 66-67 -1.1945639474390646 2308 68-69 -1.3760924241488937 2308 70-71 -1.3492269938114987 2308 72-73 -1.2734620069718332 2308 74-75 -1.0765482264745287 2308 76-77 -0.9065267037683746 2308 78-79 -1.0005668983676372 2308 80-81 -0.9516468487210119 2308 82-83 -1.1361582716152796 2308 84-85 -0.966741861554425 2308 86-87 -1.0609870998290027 2308 88-89 -1.0034192153297496 2308 90-91 -0.8261850787750902 2308 92-93 -0.8312084123857275 2308 94-95 -0.9976216292282523 2308 96-97 -1.0104668899672156 2308 98-99 -0.966821201393806 2308 100-101 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-0.11089665646035485 1213 94-95 0.05495963416312577 1213 96-97 0.0058718348184889635 1213 98-99 0.11659266184198458 1213 100-101 0.23099754128501715 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 13.0 10 27.0 11 41.0 12 69.0 13 224.0 14 652.0 15 1511.0 16 3090.0 17 5220.0 18 7560.0 19 10653.0 20 14236.0 21 18342.0 22 23509.0 23 29592.0 24 37105.0 25 46759.0 26 60791.0 27 83870.0 28 126089.0 29 166833.0 30 142508.0 31 145342.0 32 189992.0 33 262985.0 34 388138.0 35 618960.0 36 1068788.0 37 1707842.0 38 772695.0 39 4174.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.09701261082607 27.174377819903484 19.246991906081796 16.48161766318865 2 29.519426328294944 27.511413679438807 28.170230725753047 14.798929266513206 3 33.59466735111691 21.761526350997674 27.609601300994406 17.034204996891006 4 29.201335964022096 22.336276012080685 27.993149697241687 20.469238326655535 5 32.82740603461459 20.626238292431367 29.31417546313232 17.232180209821728 6 30.346846510011094 21.30316026038751 30.847199177042896 17.502794052558503 7 30.839940366598555 25.010458750083366 25.006197777827182 19.143403105490894 8 28.49470460515103 30.495643703226143 22.770349426671867 18.23930226495096 9 30.798526410954434 25.370788795226098 24.6986667367285 19.132018057090967 10-11 26.052156995101733 29.88615625271574 27.85744504693132 16.204241705251203 12-13 24.06133139046472 29.55822138597133 27.20008481524222 19.18036240832173 14-15 23.25066036649077 29.827193154419657 27.75984688794081 19.162299591148766 16-17 24.685614351358762 27.923186964725886 27.705911063235522 19.68528762067983 18-19 22.0007302599092 28.297209383166162 29.894223468963617 19.80783688796102 20-21 23.633903663627734 28.077339846389428 30.127726432781394 18.161030057201447 22-23 21.585470724594334 32.46805281315115 27.612599139182553 18.33387732307197 24-25 21.19624017197486 28.393780193114672 30.10640472971289 20.30357490519758 26-27 21.334393355443233 28.18429361837722 27.91137245074282 22.569940575436725 28-29 23.024500422055198 30.699976690965997 27.764166806453506 18.511356080525303 30-31 20.876473572203775 28.45298749733058 30.0697991044211 20.600739826044546 32-33 20.961499336770405 30.664836301193137 27.679301038868825 20.69436332316763 34-35 22.64639387012826 30.578749504009355 28.209926819064634 18.56492980679775 36-37 20.8345796256138 30.254460884274685 29.969464154949836 18.94149533516168 38-39 20.69132338051055 28.71107947100619 29.875849078720535 20.72174806976273 40-41 22.543170554088075 28.35421883410368 30.09533967527686 19.007270936531388 42-43 20.61391852482109 30.371730251151476 29.836447716691488 19.177903507335948 44-45 20.312105270603737 28.335996019948762 30.315301841885255 21.036596867562245 46-47 22.527778224553163 28.15095031089532 27.957059149814416 21.364212314737102 48-49 20.34575025764614 30.555451226436052 29.886093373757266 19.212705142160544 50-51 22.307293869696213 28.197829446345366 30.055176245591763 19.439700438366653 52-53 20.460584543377593 28.390825698889504 27.784176890603803 23.364412867129097 54-55 22.13340517598938 28.361996705938985 30.24618208862535 19.25841602944628 56-57 20.401267120319748 28.14901743263191 29.939174822127562 21.51054062492078 58-59 20.178582332190594 28.64172066991114 29.972632367079164 21.207064630819097 60-61 22.094449374583466 28.20686923222156 30.213024111720227 19.485657281474747 62-63 20.210001779376363 28.465263887072506 29.83035629548494 21.49437803806619 64-65 21.961424596491693 28.432219758678656 30.184765205920172 19.42159043890948 66-67 20.27569004418274 31.992676946353672 27.90872780682595 19.82290520263764 68-69 20.135215881978468 32.45699181134128 27.83278081351559 19.575011493164663 70-71 20.05166614951666 32.12211579376125 28.126988795532366 19.69922926118973 72-73 20.089488978281665 32.18599814001186 27.836764058601247 19.887748823105223 74-75 19.926486690452368 31.934761149125556 28.273633248769443 19.865118911652637 76-77 20.239386142214563 31.43352994419697 28.034612585526485 20.292471328061986 78-79 20.08883629406592 31.60153593950562 28.15103652168113 20.158591244747328 80-81 20.088252277655386 31.05597128579875 28.397531213312604 20.45824522323326 82-83 20.201766127290345 31.145835394713405 28.014485730056965 20.637912747939286 84-85 20.04003564150627 30.958299589243516 28.47967215607955 20.521992613170667 86-87 20.30607251714557 30.553096730234902 28.32055539600095 20.820275356618573 88-89 20.27874854980766 30.954261302025323 28.23345412128345 20.533536026883564 90-91 20.294568223013503 30.4471921636912 28.419429937019498 20.838809676275805 92-93 20.34488308761655 30.601482310505617 28.099459014022717 20.95417558785512 94-95 20.092866803322526 30.51916200052994 28.52154283780508 20.866428358342457 96-97 20.33172209967413 30.304500420706333 28.224316067949758 21.139461411669778 98-99 20.21730760305383 30.560733737665334 28.195346888504286 21.026611770776555 100-101 20.33407373362788 30.22408163117487 28.274582641550495 21.167261993646758 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24.0 1 16.5 2 18.5 3 47.0 4 47.0 5 31.0 6 34.5 7 35.5 8 40.5 9 47.0 10 56.5 11 64.5 12 86.0 13 124.5 14 146.0 15 205.0 16 330.0 17 559.5 18 941.0 19 1619.0 20 2668.0 21 4345.5 22 6813.0 23 10336.0 24 15041.5 25 21160.5 26 28860.5 27 38278.0 28 49455.5 29 62113.0 30 76440.0 31 92263.5 32 111334.0 33 133907.0 34 158760.5 35 187171.5 36 220127.0 37 255801.0 38 288296.5 39 313928.0 40 332183.0 41 336523.5 42 327342.0 43 312440.5 44 293985.5 45 275561.5 46 259425.0 47 241290.5 48 220031.5 49 198471.5 50 178037.0 51 158238.0 52 137194.0 53 115763.5 54 96004.0 55 78818.0 56 64767.0 57 52823.0 58 42342.0 59 33667.0 60 26174.5 61 19826.5 62 14868.0 63 11046.0 64 7973.5 65 5777.5 66 4254.0 67 3092.0 68 2209.0 69 1581.0 70 1158.0 71 813.0 72 570.5 73 432.0 74 330.0 75 247.5 76 186.5 77 154.5 78 121.0 79 79.5 80 58.0 81 37.5 82 33.0 83 29.5 84 22.5 85 17.0 86 12.5 87 10.5 88 6.5 89 5.0 90 4.0 91 4.0 92 3.0 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19256899911951134 2 0.0 3 0.0 4 0.025666884262562122 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 4.210446893464914E-5 48-49 0.0034525664526412303 50-51 4.210446893464914E-5 52-53 0.002644160649095966 54-55 0.009490347297869918 56-57 0.014206047818550623 58-59 0.01439130748186308 60-61 0.0035199336029366687 62-63 0.0023325875789795628 64-65 0.009945075562364129 66-67 0.017136518856402206 68-69 0.024841636671443 70-71 0.02071539871584738 72-73 0.017094414387467553 74-75 0.021422753793949485 76-77 0.015620757974754833 78-79 0.006719873241970004 80-81 0.0033936201961327216 82-83 7.494595470367549E-4 84-85 0.002568372605013598 86-87 0.015949172832445097 88-89 0.015149187922686762 90-91 0.01839965292444168 92-93 0.019140691577691504 94-95 0.017599668014683344 96-97 0.019654366098694222 98-99 0.027763686815507647 100-101 0.016142853389544482 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5937612.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.92620541132747 #Duplication Level Percentage of deduplicated Percentage of total 1 96.8484031995619 85.15512593483746 2 2.1618974083182545 3.8017487120401467 3 0.39251647608240203 1.0353745291005505 4 0.17470024329723718 0.6144291791024705 5 0.09598070266550768 0.4219609489045487 6 0.0754659535818696 0.39812609617207023 7 0.04119192125434318 0.2535294530647624 8 0.017175253497795254 0.12081238936310129 9 0.02749953921963086 0.21761371207279068 >10 0.14281138977170046 2.2942169220847815 >50 0.014644190151611827 0.856846778688195 >100 0.0073890009491572135 1.0841473621851714 >500 1.4242656468722495E-4 0.06901156264636209 >1k 1.6323449274206598E-4 0.18694168472442027 >5k 0.0 0.0 >10k+ 1.906059103930759E-5 3.49011473501307 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT 208250 3.507302262256274 TruSeq Adapter, Index 7 (97% over 35bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0012968176431871938 0.0 0.0 2 0.0015494444567950886 0.0 0.0 3 0.0018020712704029836 0.0 0.0 4 0.0019704891461415804 0.0 0.0 5 0.0024083756230619314 0.0 0.0 6 0.002930471037851581 0.0 0.0 7 0.0035873007532321076 0.0 3.3683575147719316E-5 8 0.00446307370707281 0.0 3.3683575147719316E-5 9 0.0051199034224533365 0.0 3.3683575147719316E-5 10-11 0.007048288099660268 0.0 3.3683575147719316E-5 12-13 0.009254562271835883 0.0 4.210446893464914E-5 14-15 0.011528203594306938 0.0 5.8946256508508804E-5 16-17 0.013759740447843342 0.0 1.0105072544315795E-4 18-19 0.01627758769013536 0.0 1.5157608816473693E-4 20-21 0.019073324427396066 0.0 1.5999698195166677E-4 22-23 0.02232378942915098 0.0 1.684178757385966E-4 24-25 0.025902669288596157 0.0 2.021014508863159E-4 26-27 0.029489970041828264 0.0 2.021014508863159E-4 28-29 0.03411304073085274 0.0 2.1052234467324574E-4 30-31 0.05001168820057626 0.0 2.694686011817546E-4 32-33 0.05526632592362048 0.0 3.0315217632947386E-4 34-35 0.05992308018779267 0.0 3.1157307011640367E-4 36-37 0.0652114014859846 0.0 3.368357514771932E-4 38-39 0.07167022702055978 0.0 3.620984328379827E-4 40-41 0.07796905557318329 0.0 3.705193266249125E-4 42-43 0.08558996445035479 0.0 3.7894022041184234E-4 44-45 0.09273088238167129 0.0 3.8736111419877215E-4 46-47 0.10090757024878015 0.0 3.8736111419877215E-4 48-49 0.10968214157476103 1.6841787573859658E-5 3.9578200798570196E-4 50-51 0.11900407099689235 1.6841787573859658E-5 4.126237955595617E-4 52-53 0.12902493460333886 1.6841787573859658E-5 4.547282644942108E-4 54-55 0.13963526077487043 1.6841787573859658E-5 4.6314915828114063E-4 56-57 0.15116346436917738 1.6841787573859658E-5 4.799909458550003E-4 58-59 0.16316323801555238 1.6841787573859658E-5 4.9683273342886E-4 60-61 0.1766619307560009 1.6841787573859658E-5 5.052536272157898E-4 62-63 0.1917942768911138 1.6841787573859658E-5 5.052536272157898E-4 64-65 0.207852921342789 1.6841787573859658E-5 5.052536272157898E-4 66-67 0.2265641473373471 2.526268136078949E-5 5.136745210027197E-4 68-69 0.24626903879876288 3.3683575147719316E-5 5.641998837242986E-4 70-71 0.2683149387329452 3.3683575147719316E-5 5.726207775112284E-4 72-73 0.2919439666990703 3.3683575147719316E-5 5.726207775112284E-4 74-75 0.3190845073743451 3.3683575147719316E-5 5.726207775112284E-4 76-77 0.3489028922738636 3.3683575147719316E-5 5.894625650850881E-4 78-79 0.3817696407242508 3.3683575147719316E-5 6.063043526589477E-4 80-81 0.4196047165089265 3.3683575147719316E-5 6.063043526589477E-4 82-83 0.46105235572819514 3.3683575147719316E-5 6.063043526589477E-4 84-85 0.5082598862977237 3.3683575147719316E-5 6.147252464458776E-4 86-87 0.5610841530231345 3.3683575147719316E-5 6.231461402328074E-4 88-89 0.6185651740127176 3.3683575147719316E-5 6.231461402328074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGAAG 28865 0.0 78.66719 3 CGGAAGA 29045 0.0 77.96704 4 ATCGGAA 29360 0.0 77.38943 2 GATCGGA 29440 0.0 77.38708 1 GAAGAGC 31435 0.0 72.0041 6 AGAGCAC 32970 0.0 68.63736 8 GAGCACA 33020 0.0 68.49027 9 AAGAGCA 36125 0.0 62.563988 7 GGAAGAG 38630 0.0 58.949547 5 TATGCCG 24900 0.0 44.836376 48-49 GCCGTCT 24910 0.0 44.764946 50-51 TGCCGTC 24910 0.0 44.755413 50-51 CGTATGC 24985 0.0 44.71029 46-47 TCTCGTA 24980 0.0 44.557247 42-43 CTCGTAT 24975 0.0 44.55666 44-45 ATGCCGT 25060 0.0 44.455345 48-49 CGTCTTC 25165 0.0 44.379272 52-53 TCGTATC 24865 0.0 44.321014 38-39 ATTCGTA 24770 0.0 44.251335 36-37 TATCTCG 24915 0.0 44.25113 40-41 >>END_MODULE