FastQCFastQC Report
Thu 15 Oct 2015
C68Y0ACXX_l08n01_35008-2.341000000358b3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC68Y0ACXX_l08n01_35008-2.341000000358b3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5937612
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT2082503.507302262256274TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG288650.078.667193
CGGAAGA290450.077.967044
ATCGGAA293600.077.389432
GATCGGA294400.077.387081
GAAGAGC314350.072.00416
AGAGCAC329700.068.637368
GAGCACA330200.068.490279
AAGAGCA361250.062.5639887
GGAAGAG386300.058.9495475
TATGCCG249000.044.83637648-49
GCCGTCT249100.044.76494650-51
TGCCGTC249100.044.75541350-51
CGTATGC249850.044.7102946-47
TCTCGTA249800.044.55724742-43
CTCGTAT249750.044.5566644-45
ATGCCGT250600.044.45534548-49
CGTCTTC251650.044.37927252-53
TCGTATC248650.044.32101438-39
ATTCGTA247700.044.25133536-37
TATCTCG249150.044.2511340-41
Produced by FastQC (version 0.11.2)